Physicochemical Evaluation of Biofield Treated Peptone and Malmgren Modified Terrestrial Orchid Medium
American Journal of Bioscience and Bioengineering
Volume 3, Issue 6, December 2015, Pages: 169-177
Received: Oct. 29, 2015;
Accepted: Dec. 3, 2015;
Published: Dec. 21, 2015
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Mahendra Kumar Trivedi, Trivedi Global Inc., Henderson, USA
Alice Branton, Trivedi Global Inc., Henderson, USA
Dahryn Trivedi, Trivedi Global Inc., Henderson, USA
Gopal Nayak, Trivedi Global Inc., Henderson, USA
Rakesh Kumar Mishra, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Peptone and Malmgren modified terrestrial orchid (MMTO) has been used as a growth medium for tissue culture applications. This research study was conducted to explore the influence of Mr. Trivedi’s biofield energy treatment on physicochemical properties of peptone and MMTO. The study was performed in two groups i.e. control and treated. The control group was kept aside as untreated, and the treated group was received the biofield energy treatment. The control and treated samples were further subjected to characterization by X-ray diffraction (XRD), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), Fourier transform infrared (FT-IR) spectroscopy, particle size analyzer and surface area analyzer. The XRD analysis revealed the amorphous nature of the control and treated peptone samples. The DSC analysis showed an increase in thermal denaturation temperature of the treated peptone (196.22°C) as compared to the control sample (141.20°C). Additionally, the exothermic peak of treated sample (280°C) was increased as compared to the control (270°C). The DSC of control and treated MMTO showed the absence of the melting temperature in their respective DSC thermograms. The TGA analysis of the treated peptone showed an increase in onset of thermal degradation (172°C) with respect to the control (170°C). Nevertheless, the TGA thermogram of the treated MMTO (293.96°C) showed an increase in maximum thermal degradation temperature (Tmax) as compared with the control (281.41°C). It indicated the good thermal stability of the treated peptone and MMTO samples. The FT-IR result of the treated peptone showed an upward shift in C-H (2817→2833 cm-1), and amide I (1635→1641 cm-1), stretching in the treated sample with respect to the control sample. Whereas, the FT-IR spectrum of the treated MMTO showed an increase in the frequency of the C-H (2817→2833 cm-1) and amide I (1596→1606 cm-1) bands as compared to the control. Particle size analysis of the treated peptone showed an increase in d50 (average particle size) and d99 (size exhibited by 99% of particles) by 9.3 and 41.4%, respectively with respect to the control. Surface area analysis showed increase in surface area by 4.3% in the treated peptone. Altogether, the results corroborated that the biofield energy treatment had altered the physical, thermal and spectral properties of peptone and MMTO. It is assumed that biofield treated peptone and MMTO could be utilized as potential candidates for cell culture applications.
Mahendra Kumar Trivedi,
Rakesh Kumar Mishra,
Physicochemical Evaluation of Biofield Treated Peptone and Malmgren Modified Terrestrial Orchid Medium, American Journal of Bioscience and Bioengineering.
Vol. 3, No. 6,
2015, pp. 169-177.
Surachi U (1999) Basic techniques in animal cell culture. Drug Delivery System Workshop, Bangkok.
Neema R, Khare S (2012) An animal cell culture: Advance technology for modern research. Adv Biosci Biotechnol 3: 219-226.
Madigan M, Martinko J (2005) Brock Biology of Microorganisms (11thedn), Prentice Hall, NJ.
https://en.wikipedia.org/wiki/Growth_medium (Accessed on 26 October 2015).
http://himedialabs.com/TD/RM001.pdf (Accessed on 26 October 2015).
Wijayanti HB, Bansal N, Deeth HC (2014) Stability of whey proteins during thermal processing: A Review. Compr Rev Food Sci F 13: 1235-1251.
Marshall G, Valtchev P, Dehghani F, Gomes VG (2015) Thermal denaturation and protein stability analysis of Haliotis rubrahemocyanin. J Therm Anal Calorim 1-7 (In Press).
Bischof JC, He X (2005) Thermal stability of proteins. Ann N Y Acad Sci 1066: 12-33.
http://himedialabs.com/TD/PT068.pdf (Accessed on 26 October 2015).
Uchida S, Iha T, Yamaoka K, Nitta K, Sugano H (2012) Effect of biofield therapy in the human brain. J Altern and Complement Med 18: 875-879.
Wilson CA (2011) Healing power beyond medicine. John Hunt Publishing Ltd, UK.
Warber SL, Cornelio D, Straughn J, Kile G (2004) Biofield energy healing from the inside. J Altern Complement Med 10: 1107-1113.
Prakash S, Chowdhury AR, Gupta A (2015) Monitoring the human health by measuring the biofield "aura": An overview. IJAER 10: 27637-27641.
Barnes PM, Powell-Griner E, McFann K, Nahin RL (2004) Complementary and alternative medicine use among adults: United States, 2002. Adv Data 343: 1-19.
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Phenotypic and biotypic characterization of Klebsiella oxytoca: An impact of biofield treatment. J Microb Biochem Technol 7: 203-206.
Nayak G, Altekar N (2015) Effect of biofield treatment on plant growth and adaptation. J Environ Health Sci 1: 1-9.
Trivedi MK, Patil S, Tallapragada RMR (2015) Effect of biofield treatment on the physical and thermal characteristics of aluminium powders. Ind Eng Manag 4: 151.
Trivedi MK, Patil S, Shettigar H, Bairwa K, Jana S (2015) Effect of biofield treatment on spectral properties of paracetamol and piroxicam. Chem Sci J 6: 98.
Trivedi MK, Nayak G, Patil S, Tallapragada RM, Jana S, et al. (2015) Bio-field treatment: An effective strategy to improve the quality of beef extract and meat infusion powder. J Nutr Food Sci 5: 389.
Namazu T, Takemoto H, Inoue S (2010) Tensile and creep characteristics of sputtered gold tin eutectic solder film evaluated by XRD tensile testing. Sensor Mater 22: 13-24.
Tang CH, Chen Z, Li L, Yang XQ (2006) Effects of transglutaminase treatment on the thermal properties of soy protein isolates. Food Res Int 39: 704-711.
Bell LN, Touma DE (1996) Glass transition temperatures determined using a temperature cycling differential scanning calorimeter. J Food Sci 61: 807-810.
Gioia LD, Cuq B, Guilbert S (1999) Thermal properties of corn gluten meal and its proteic components. Int J Biol Macromol 24: 341-350.
Tang C, Yang XQ, Chen Z, Wu H, Peng ZY (2005) Physicochemical and structural characteristics of sodium caseinate biopolymers induced by microbial transglutaminase. J Food Biochem 29: 402-421.
Arntfield SD, Murray ED (1981) The influence of processing parameters on food protein functionality I. Differential scanning calorimetry as an indicator of protein denaturation. Can I Food Sc Tech J 14: 289-294.
Privalov PL (1982) Stability of proteins. Proteins which do not present a single cooperative system. Adv Protein Chem 35: 1-104.
Szabo L, Cik G, Lensy J (1996) Thermal stability increase of doped poly (hexadecylthiophene) by γ-radiation. Synt Met 78: 149-153.
Pavia DL, Lampman GM, Kriz GS (2001) Introduction to spectroscopy. (3rdedn), Thomson Learning, Singapore.
Agarry SE, Ogunleye OO (2015) Bioadsorption of 2, 6-dichlorophenol from aqueous solution onto plantain and pineapple peels mixture used as adsorbent: Optimization studies based on taguchi method, batch equilibrium, and kinetic modelling. Chem Mater Res 7: 1-14.
Midelfort KS, Wittrup KD (2006) Context-dependent mutations predominate in an engineered high-affinity single chain antibody fragment. Protein Sci 15: 324-334.
Hooper NM (1994) Families of zinc metalloproteases. FEBS Lett 354: 1-6.
Wiseman RL, Powers ET, Kelly JW (2005) Partitioning conformational intermediates between competing refolding and aggregation pathways: Insights into transthyretin amyloid disease. Biochemistry 44: 16612-16623.
Amin S, Barnett GV, Pathak JA, Roberts CJ, Sarangapani PS (2014) Protein aggregation, particle formation, characterization & rheology. Curr Opin Colloid Interface Sci 19: 438-449.
Trivedi MK, Patil S, Mishra RK, Jana S (2015) Thermal and physical properties of biofield treated bile salt and proteose peptone. J Anal Bioanal Tech 6: 256.