Increased Cell Proliferation by Various Doses of Xenoestrogen in MFC-7 Cell Through Tyrosine Phosphatase Shp2
American Journal of Bioscience and Bioengineering
Volume 3, Issue 6, December 2015, Pages: 178-182
Received: Dec. 23, 2015;
Published: Dec. 23, 2015
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Wenjie Liu, School of Pharmaceutical Sciences, Xiamen University, Xiamen, China
Qinyuan Wang, School of Pharmaceutical Sciences, Xiamen University, Xiamen, China; College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, China
Zhaokai Wang, Third Institute of Oceanography, State Oceanic Administration, Xiamen, China
Defeng Wu, College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, China
Wenhua Liu, Third People's Hospital of Pingdu, Pingdu, China
Juan Wang, Third People's Hospital of Pingdu, Pingdu, China
Our previous study demonstrated that tyrosine phosphatase Shp2 mediates the estrogen biological action in breast cancer. However, consumer research suggests that the Xenoestrogens exposure in breast tumorigenesisis more important. The present study examined the consequences of different concentrations xenoestrogens exposure to Bisphenol A (BPA) and Nonyl Phenol (NP) on cell proliferation and shp2 expression in MCF-7 cell. Phps1 (2x10-8 M), BPA (10-12, 10-11, 10-10, 10-9, 10-8, 10-7, 10-6 M), N P (10-12, 10-11, 10-10, 10-9, 10-8, 10-7, 10-6 M) and was administered in MCF-7 cell, respectively. Results showed the exposure of various concentrations xenoestrogens significantly elevated cell proliferation (P<0.05). Proliferation of MCF-7 cells was down regulated by Phps1 (a Shp2 inhibitor) when cells were treated for 1 h. In addition, the tyrosine phosphatase Shp2 levels were higher in BPA- and NP-exposed indicating that Shp2 plays a crutial role for Xenoestrogens exposure facilitating breast tumorigenesis properties.
Increased Cell Proliferation by Various Doses of Xenoestrogen in MFC-7 Cell Through Tyrosine Phosphatase Shp2, American Journal of Bioscience and Bioengineering.
Vol. 3, No. 6,
2015, pp. 178-182.
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