Micro-Technological Steps During the Fabrication of an AcHE Biosensor Designated to the Environment Monitoring
American Journal of Bioscience and Bioengineering
Volume 3, Issue 3-1, June 2015, Pages: 1-6
Received: Feb. 14, 2015; Accepted: Feb. 14, 2015; Published: Mar. 12, 2015
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Cristian Ravariu, Dept. of Electronics, Polytechnic University of Bucharest, BioNEC Group, Bucharest, Romania
Elena Manea, Institute of Microtechnology, Bucharest, Romania
Alina Popescu, Institute of Microtechnology, Bucharest, Romania
Cecilia Podaru, Institute of Microtechnology, Bucharest, Romania
Catalin Parvulescu, Institute of Microtechnology, Bucharest, Romania
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Unfortunately, the pesticides pollute a large palette from environment: plants, organisms, soil and water. Using monitoring tools, like chemical biosensors, the use of pesticides can be put under control. Microelectronics offers the convenient transducers. Borrowing micro-technological processes, the enzymatic biosensors can be easily integrated onto the Silicon wafers. This paper proposes a biosensor for paraoxon pesticide detection, as a small piece from a decontamination soil technology plan. The paper reveals the pesticide detection principle, by paraoxon hydrolysis assisted by the Acetyl-cholinesterase enzyme, as key receptor. The enzyme is entrapped on a porous thin layer by adsorption. An advantageous method of the porous intermediate material anchored onto the Si-substrate, converting p-type Si in Si-porous by anodization, is described. Then, some technological steps, with tests and microscopy analysis are presented. Finally, the preliminary tests of the developed biosensor with the AcHE enzyme immobilized onto the Si-porous layer, are discussed.
Biosensor, Design, Enzyme, Si-porous, Electrodes
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Cristian Ravariu, Elena Manea, Alina Popescu, Cecilia Podaru, Catalin Parvulescu, Micro-Technological Steps During the Fabrication of an AcHE Biosensor Designated to the Environment Monitoring, American Journal of Bioscience and Bioengineering. Special Issue:Bio-Electronics: Biosensors, Biomedical Signal Processing, and Organic Engineering. Vol. 3, No. 3-1, 2015, pp. 1-6. doi: 10.11648/j.bio.s.2015030301.11
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