American Journal of Agriculture and Forestry

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Spore Culture of Nephrolepis falcate (Cav). C. Chr.

Received: 28 March 2015    Accepted: 30 June 2015    Published: 10 November 2015
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Abstract

Spore culture of Nephrolepis falcate (Cav). C. Chr. was undertaken to propagate Nephrolepis falcata in vitro using the spores. Specifically, it aimed to find out the duration of the germination of Nephrolepis falcate spores and observe spore germination and prothallial development. Nephrolepis falcate is a fern plant under family Lomariopsidaceae which is found on shaded areas (Smith et al., 2006). Its rhizome is creeping, frond is simple pinnate, sori round and spores bilateral, monolete. This species was used as ornamental. Thus, there is a need to conduct an in vitro (spore) culture of this plant. In vitro culture of this species revealed a rapid spore germination of 3 days after inoculation. Germ filament formation of one to twelve cells was observed from 3rd to 16th day after inoculation, and prothallial plate formation was noticed 19th day after inoculation. Correspondingly, Nephrolepis falcate (Cav.) C. Chr. spore germination is of Vittaria type and its prothallial development follows Adiantum-type.

DOI 10.11648/j.ajaf.s.2015030601.13
Published in American Journal of Agriculture and Forestry (Volume 3, Issue 6-1, December 2015)

This article belongs to the Special Issue Agro-Ecosystems

Page(s) 10-14
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Monolete, In Vitro, Germination, Prothallial Development, Inoculation, Spore Culture

References
[1] Amoroso, V. B. 2006. Pteridophyte and Gymnosperm Diversity In Musuan, Bukidnon. Philippine Journal of Systematic Biology Vol. I, No.
[2] Herrero, A., C. Prada and S. Pajarón. 2002. Gametophyte Morphology and Gametangial Ontogeny of Asplenium Foreziense and Related Taxa (Aspleniaceae: Pteridophyta). Botanical Journal of the Linnean Society, 139, 87–98.
[3] Nayar, B. K. and S. Kaur. 1971. Gametophyte of Homosphorous ferns. Botanical Review. 37(3): 295 – 385.
[4] Kramer, K. U., P. S. Green and E. Götz. 1990. Pteridophytes and Gymnosperms. 79 – 82.
[5] Smith, A. R., K.M. Pryer, E. Schuettpelz, P. Korall, H. Schneider, and P.G. Wolf. 2006. A classification for extant ferns. TAXON 55(3): 705-731.
[6] Amorsolo, C.B. 2009. Spore tissue culture of platycerium grande. Central Mindanao University Pamphlet. CMU University Town. Musuan, Bukidnon, Philippines.
[7] Haddad, S. and R. Bayerly. 2014. In vitro propagation of ferns (Asplenium nidus) via spore culture. Jordan Journal of Agricultural Sciences. 10(1):144-153.
[8] Bharati, S. K., D.C. Manabendra and M.P. Behari. 2013. In Vitro propagation in Pteridophytes: A Review. International J. Res. Ayurveda Pharm. 4(2):297-303.
[9] Chang, H.C., D.C. Agrawal, C.L. Kuo, J.L. Wen, C. Chen and H.S. Tsay. In Vitro culture of Drynara fortune, a fern species source of chinese medicine “Gu-Sui-Bu”. In Vitro Cell. Dev. Biol. - Plant. 43(133-139.
[10] Khan, S.M. Rasiq and H.A. Kagani. 2008. In Vitro propagation of birds Nest Fern (Asplenium nidus) from spores. Pak. J. Bot. 40(1): 91-97.
Author Information
  • Science Laboratory, College of Education, Cotabato Foundation College of Science and Technology, Arakan, Cotabato Philippines

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  • APA Style

    Farida D. Silverio. (2015). Spore Culture of Nephrolepis falcate (Cav). C. Chr.. American Journal of Agriculture and Forestry, 3(6-1), 10-14. https://doi.org/10.11648/j.ajaf.s.2015030601.13

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    Farida D. Silverio. Spore Culture of Nephrolepis falcate (Cav). C. Chr.. Am. J. Agric. For. 2015, 3(6-1), 10-14. doi: 10.11648/j.ajaf.s.2015030601.13

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    AMA Style

    Farida D. Silverio. Spore Culture of Nephrolepis falcate (Cav). C. Chr.. Am J Agric For. 2015;3(6-1):10-14. doi: 10.11648/j.ajaf.s.2015030601.13

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  • @article{10.11648/j.ajaf.s.2015030601.13,
      author = {Farida D. Silverio},
      title = {Spore Culture of Nephrolepis falcate (Cav). C. Chr.},
      journal = {American Journal of Agriculture and Forestry},
      volume = {3},
      number = {6-1},
      pages = {10-14},
      doi = {10.11648/j.ajaf.s.2015030601.13},
      url = {https://doi.org/10.11648/j.ajaf.s.2015030601.13},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajaf.s.2015030601.13},
      abstract = {Spore culture of Nephrolepis falcate (Cav). C. Chr. was undertaken to propagate Nephrolepis falcata in vitro using the spores. Specifically, it aimed to find out the duration of the germination of Nephrolepis falcate spores and observe spore germination and prothallial development. Nephrolepis falcate is a fern plant under family Lomariopsidaceae which is found on shaded areas (Smith et al., 2006). Its rhizome is creeping, frond is simple pinnate, sori round and spores bilateral, monolete. This species was used as ornamental. Thus, there is a need to conduct an in vitro (spore) culture of this plant. In vitro culture of this species revealed a rapid spore germination of 3 days after inoculation. Germ filament formation of one to twelve cells was observed from 3rd to 16th day after inoculation, and prothallial plate formation was noticed 19th day after inoculation. Correspondingly, Nephrolepis falcate (Cav.) C. Chr. spore germination is of Vittaria type and its prothallial development follows Adiantum-type.},
     year = {2015}
    }
    

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    AU  - Farida D. Silverio
    Y1  - 2015/11/10
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    T2  - American Journal of Agriculture and Forestry
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    JO  - American Journal of Agriculture and Forestry
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    AB  - Spore culture of Nephrolepis falcate (Cav). C. Chr. was undertaken to propagate Nephrolepis falcata in vitro using the spores. Specifically, it aimed to find out the duration of the germination of Nephrolepis falcate spores and observe spore germination and prothallial development. Nephrolepis falcate is a fern plant under family Lomariopsidaceae which is found on shaded areas (Smith et al., 2006). Its rhizome is creeping, frond is simple pinnate, sori round and spores bilateral, monolete. This species was used as ornamental. Thus, there is a need to conduct an in vitro (spore) culture of this plant. In vitro culture of this species revealed a rapid spore germination of 3 days after inoculation. Germ filament formation of one to twelve cells was observed from 3rd to 16th day after inoculation, and prothallial plate formation was noticed 19th day after inoculation. Correspondingly, Nephrolepis falcate (Cav.) C. Chr. spore germination is of Vittaria type and its prothallial development follows Adiantum-type.
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