The Effect of Growth Differentiation Factor-5, 6, 7 in Chondrogenic Cell Differentiation of ATDC-5
American Journal of BioScience
Volume 2, Issue 5, September 2014, Pages: 182-186
Received: Jul. 31, 2014; Accepted: Aug. 25, 2014; Published: Sep. 10, 2014
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Authors
Yuji a Hatakeyam, Section of Functional Structure, Department of Morphological Biology, Fukuoka Dental College, Fukuoka, Japan
Yuko Matsuda, Section of Orthodontics, Department of Oral Growth and Development, Fukuoka Dental College, Fukuoka, Japan
Junko Hatakeyama, Section of Operative Dentistry, Department of Odontology, Fukuoka Dental College, Fukuoka, Japan
Kyoko Oka, Section of Pediatric Dentistry, Department of Oral Growth and Development, Fukuoka Dental College, Fukuoka, Japan
Hisashi Anan, Section of Operative Dentistry, Department of Odontology, Fukuoka Dental College, Fukuoka, Japan
Eichi Tsuruga, Department of Pathologic Aanalysis, Division of Medical Sciences, Hirosaki University Graduate School of Health Sciences, Hirosaki, Japan
Tetsuichiro Inai, Section of Functional Structure, Department of Morphological Biology, Fukuoka Dental College, Fukuoka, Japan
Hiroyuki Ishikawa, Section of Orthodontics, Department of Oral Growth and Development, Fukuoka Dental College, Fukuoka, Japan
Yoshihiko Sawa, Section of Functional Structure, Department of Morphological Biology, Fukuoka Dental College, Fukuoka, Japan
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Abstract
The proteins known as growth differentiation factors (GDFs) are members of the BMP family; GDF-5, -6, and -7 play important roles in skeletogenesis, especially with regard to chondrogenesis. The functional differences among these GDFs in chondrogenesis and chondrocyte cell differentiation remains unclear. Here, we attempt to assay cell proliferation, the production of chondrogenic matrices indicated by alcian blue intensity, and the profile of a chondrogenic cell differentiation marker gene in a cell culture of the chondrogenic cell line ATDC5 with or without recombinant mouse GDF-5, -6, and -7. After 24h culture, the ATDC5 cell number was significantly decreased in the exogenous presence of each GDF compared with a control culture absent all GDFs. On the other hand, alcian blue staining of cell cultures after 2w culture showed significantly increased intensity compared with that of the control. The expression levels of the chondrogenic cell differentiation marker genes Sox9 and aggrecan were increased after 24h and 48h culture by all GDFs, but were significantly increased in the presence of GDF-5 compared with the presence of GDF-6 or -7. These findings suggest that GDF-5, -6, and -7 could all promote chondrogenic cell differentiation of ATDC5, but GDF-5 may induce chondrogenic genes more potently than GDF-6 and -7 do.
Keywords
Growth Differentiation Factors (GDFs), Chondrogenesis, ATDC-5
To cite this article
Yuji a Hatakeyam, Yuko Matsuda, Junko Hatakeyama, Kyoko Oka, Hisashi Anan, Eichi Tsuruga, Tetsuichiro Inai, Hiroyuki Ishikawa, Yoshihiko Sawa, The Effect of Growth Differentiation Factor-5, 6, 7 in Chondrogenic Cell Differentiation of ATDC-5, American Journal of BioScience. Vol. 2, No. 5, 2014, pp. 182-186. doi: 10.11648/j.ajbio.20140205.13
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