Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor

Xiarong Zhou; Shangquan Wu; Hong Liu; Xiaoping Wu; Qingchuan Zhang

doi:doi:10.11648/j.ijmea.20160402.17

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Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor

Xiarong Zhou, Shangquan Wu, Hong Liu, Xiaoping Wu, Qingchuan Zhang

Published in International Journal of Mechanical Engineering and Applications (Volume 4, Issue 2)

Received: 7 May 2016 Accepted: Published: 9 May 2016

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Abstract

As a highly sensitive nanomechanical sensor, microcantilever sensor is widely used in biochemical detection. Aflatoxin B1 (AFB1), a hepatocarcinogen widely present in food and food materials, is highly dangerous to human health, and new sensitive methods to detect AFB1 are needed. Here, we developed a microcantilever-array-based immunosensor used in stress mode to detect AFB1with the obvious advantages of a high sensitivity, rapidity, label-free, quantitative, and ability to be performed in real-time. The microcantilever was functionalized with a sulfhydrylated anti-AFB1 antibody, and an ELISA was used to validate the activity of the antibody on the microcantilever. Deflection of the microcantilever corresponding to different AFB1 concentrations was monitored in real-time. The detection limit of the microcantilever sensor in stress mode was lowered to 0.03 ng/mL for AFB1, which is a significant improvement in comparison with icELISA or a microcantilever sensor operated in dynamic mode. We also successfully detected AFB1 dissolved in a peanut solution. The microcantilever sensor in stress mode provides a new method for detecting extremely low concentrations of AFB1 and may have great potential for food quality control and public health protection.

Published in	International Journal of Mechanical Engineering and Applications (Volume 4, Issue 2)
DOI	10.11648/j.ijmea.20160402.17
Page(s)	81-87
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Microcantilever, Nanomechanical Sensor, Aflatoxin B1, Label-Free, Surface Stress

References

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APA Style

Xiarong Zhou, Shangquan Wu, Hong Liu, Xiaoping Wu, Qingchuan Zhang. (2016). Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor. International Journal of Mechanical Engineering and Applications, 4(2), 81-87. https://doi.org/10.11648/j.ijmea.20160402.17

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ACS Style

Xiarong Zhou; Shangquan Wu; Hong Liu; Xiaoping Wu; Qingchuan Zhang. Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor. Int. J. Mech. Eng. Appl. 2016, 4(2), 81-87. doi: 10.11648/j.ijmea.20160402.17

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AMA Style

Xiarong Zhou, Shangquan Wu, Hong Liu, Xiaoping Wu, Qingchuan Zhang. Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor. Int J Mech Eng Appl. 2016;4(2):81-87. doi: 10.11648/j.ijmea.20160402.17

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@article{10.11648/j.ijmea.20160402.17,
  author = {Xiarong Zhou and Shangquan Wu and Hong Liu and Xiaoping Wu and Qingchuan Zhang},
  title = {Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor},
  journal = {International Journal of Mechanical Engineering and Applications},
  volume = {4},
  number = {2},
  pages = {81-87},
  doi = {10.11648/j.ijmea.20160402.17},
  url = {https://doi.org/10.11648/j.ijmea.20160402.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmea.20160402.17},
  abstract = {As a highly sensitive nanomechanical sensor, microcantilever sensor is widely used in biochemical detection. Aflatoxin B1 (AFB1), a hepatocarcinogen widely present in food and food materials, is highly dangerous to human health, and new sensitive methods to detect AFB1 are needed. Here, we developed a microcantilever-array-based immunosensor used in stress mode to detect AFB1with the obvious advantages of a high sensitivity, rapidity, label-free, quantitative, and ability to be performed in real-time. The microcantilever was functionalized with a sulfhydrylated anti-AFB1 antibody, and an ELISA was used to validate the activity of the antibody on the microcantilever. Deflection of the microcantilever corresponding to different AFB1 concentrations was monitored in real-time. The detection limit of the microcantilever sensor in stress mode was lowered to 0.03 ng/mL for AFB1, which is a significant improvement in comparison with icELISA or a microcantilever sensor operated in dynamic mode. We also successfully detected AFB1 dissolved in a peanut solution. The microcantilever sensor in stress mode provides a new method for detecting extremely low concentrations of AFB1 and may have great potential for food quality control and public health protection.},
 year = {2016}
}

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TY  - JOUR
T1  - Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor
AU  - Xiarong Zhou
AU  - Shangquan Wu
AU  - Hong Liu
AU  - Xiaoping Wu
AU  - Qingchuan Zhang
Y1  - 2016/05/09
PY  - 2016
N1  - https://doi.org/10.11648/j.ijmea.20160402.17
DO  - 10.11648/j.ijmea.20160402.17
T2  - International Journal of Mechanical Engineering and Applications
JF  - International Journal of Mechanical Engineering and Applications
JO  - International Journal of Mechanical Engineering and Applications
SP  - 81
EP  - 87
PB  - Science Publishing Group
SN  - 2330-0248
UR  - https://doi.org/10.11648/j.ijmea.20160402.17
AB  - As a highly sensitive nanomechanical sensor, microcantilever sensor is widely used in biochemical detection. Aflatoxin B1 (AFB1), a hepatocarcinogen widely present in food and food materials, is highly dangerous to human health, and new sensitive methods to detect AFB1 are needed. Here, we developed a microcantilever-array-based immunosensor used in stress mode to detect AFB1with the obvious advantages of a high sensitivity, rapidity, label-free, quantitative, and ability to be performed in real-time. The microcantilever was functionalized with a sulfhydrylated anti-AFB1 antibody, and an ELISA was used to validate the activity of the antibody on the microcantilever. Deflection of the microcantilever corresponding to different AFB1 concentrations was monitored in real-time. The detection limit of the microcantilever sensor in stress mode was lowered to 0.03 ng/mL for AFB1, which is a significant improvement in comparison with icELISA or a microcantilever sensor operated in dynamic mode. We also successfully detected AFB1 dissolved in a peanut solution. The microcantilever sensor in stress mode provides a new method for detecting extremely low concentrations of AFB1 and may have great potential for food quality control and public health protection.
VL  - 4
IS  - 2
ER  -

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Author Information

Xiarong Zhou

Department of Modern Mechanics, University of Science and Technology of China, Hefei, People’s Republic of China
Shangquan Wu

Department of Modern Mechanics, University of Science and Technology of China, Hefei, People’s Republic of China
Hong Liu

Department of Chemical Physics, University of Science and Technology of China, Hefei, People’s Republic of China
Xiaoping Wu

Department of Modern Mechanics, University of Science and Technology of China, Hefei, People’s Republic of China
Qingchuan Zhang

Department of Modern Mechanics, University of Science and Technology of China, Hefei, People’s Republic of China

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APA Style

Xiarong Zhou, Shangquan Wu, Hong Liu, Xiaoping Wu, Qingchuan Zhang. (2016). Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor. International Journal of Mechanical Engineering and Applications, 4(2), 81-87. https://doi.org/10.11648/j.ijmea.20160402.17

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ACS Style

Xiarong Zhou; Shangquan Wu; Hong Liu; Xiaoping Wu; Qingchuan Zhang. Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor. Int. J. Mech. Eng. Appl. 2016, 4(2), 81-87. doi: 10.11648/j.ijmea.20160402.17

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AMA Style

Xiarong Zhou, Shangquan Wu, Hong Liu, Xiaoping Wu, Qingchuan Zhang. Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor. Int J Mech Eng Appl. 2016;4(2):81-87. doi: 10.11648/j.ijmea.20160402.17

Copy | Download

@article{10.11648/j.ijmea.20160402.17,
  author = {Xiarong Zhou and Shangquan Wu and Hong Liu and Xiaoping Wu and Qingchuan Zhang},
  title = {Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor},
  journal = {International Journal of Mechanical Engineering and Applications},
  volume = {4},
  number = {2},
  pages = {81-87},
  doi = {10.11648/j.ijmea.20160402.17},
  url = {https://doi.org/10.11648/j.ijmea.20160402.17},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmea.20160402.17},
  abstract = {As a highly sensitive nanomechanical sensor, microcantilever sensor is widely used in biochemical detection. Aflatoxin B1 (AFB1), a hepatocarcinogen widely present in food and food materials, is highly dangerous to human health, and new sensitive methods to detect AFB1 are needed. Here, we developed a microcantilever-array-based immunosensor used in stress mode to detect AFB1with the obvious advantages of a high sensitivity, rapidity, label-free, quantitative, and ability to be performed in real-time. The microcantilever was functionalized with a sulfhydrylated anti-AFB1 antibody, and an ELISA was used to validate the activity of the antibody on the microcantilever. Deflection of the microcantilever corresponding to different AFB1 concentrations was monitored in real-time. The detection limit of the microcantilever sensor in stress mode was lowered to 0.03 ng/mL for AFB1, which is a significant improvement in comparison with icELISA or a microcantilever sensor operated in dynamic mode. We also successfully detected AFB1 dissolved in a peanut solution. The microcantilever sensor in stress mode provides a new method for detecting extremely low concentrations of AFB1 and may have great potential for food quality control and public health protection.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Label-Free Detection of Aflatoxin B1 Using a Nanomechanical Sensor
AU  - Xiarong Zhou
AU  - Shangquan Wu
AU  - Hong Liu
AU  - Xiaoping Wu
AU  - Qingchuan Zhang
Y1  - 2016/05/09
PY  - 2016
N1  - https://doi.org/10.11648/j.ijmea.20160402.17
DO  - 10.11648/j.ijmea.20160402.17
T2  - International Journal of Mechanical Engineering and Applications
JF  - International Journal of Mechanical Engineering and Applications
JO  - International Journal of Mechanical Engineering and Applications
SP  - 81
EP  - 87
PB  - Science Publishing Group
SN  - 2330-0248
UR  - https://doi.org/10.11648/j.ijmea.20160402.17
AB  - As a highly sensitive nanomechanical sensor, microcantilever sensor is widely used in biochemical detection. Aflatoxin B1 (AFB1), a hepatocarcinogen widely present in food and food materials, is highly dangerous to human health, and new sensitive methods to detect AFB1 are needed. Here, we developed a microcantilever-array-based immunosensor used in stress mode to detect AFB1with the obvious advantages of a high sensitivity, rapidity, label-free, quantitative, and ability to be performed in real-time. The microcantilever was functionalized with a sulfhydrylated anti-AFB1 antibody, and an ELISA was used to validate the activity of the antibody on the microcantilever. Deflection of the microcantilever corresponding to different AFB1 concentrations was monitored in real-time. The detection limit of the microcantilever sensor in stress mode was lowered to 0.03 ng/mL for AFB1, which is a significant improvement in comparison with icELISA or a microcantilever sensor operated in dynamic mode. We also successfully detected AFB1 dissolved in a peanut solution. The microcantilever sensor in stress mode provides a new method for detecting extremely low concentrations of AFB1 and may have great potential for food quality control and public health protection.
VL  - 4
IS  - 2
ER  -

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