A new approach has been developed and validated for the mutual determination of the drugs of amoxicillin (AMX) and Metoclopramide hydrochloride (MCP. HCl) in pharmaceuticals. The method is based on the reaction of diazotized Metoclopramide with amoxicillin in an alkaline medium to form an intense orange water-soluble product which can be easily extracted from micelles of a non-ionic surfactant (Triton X-114) and both drugs measured sequentially at the same absorption maximum of 479 nm. The optimization of all experimental variables was individually performed to obtain high extraction efficiency for both target medicaments. Under the optimized conditions, Beer’s law was obeyed in the concentration range of 0.3-3.0 μg mL-1 (r=0.9995) for both AMX and MCP. The enrichment factors were found to 214 and 90.85 fold for AMX and MCP, led to obtaining the detection limits of 0.083 and 0.098 μg mL5, and a superb sensitivity in terms of the molar absorptivity of 2.35x105 and 2.25x10-1 L.mol5.cm-1, respectively. The mean recovery percentage of 97.77±1.72% (in AMX capsule) and 98.20±1.95 (in MCP ampoule); the precision (RSD %) ranged between 2.35-10.8% and 0.20-3.43% were obtained for AMX and MCP respectively. The proposed method was validated and applied for determination of AMX and MCP in various samples of the pharmaceutical preparations.
| DOI | 10.11648/j.sjac.20160405.12 |
| Published in | Science Journal of Analytical Chemistry (Volume 4, Issue 5, September 2016) |
| Page(s) | 66-76 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Amoxicillin, Metoclopramide Hydrochloride, Diazotization Coupling Reaction, Cloud Poin Extraction, Visible Spectrophotometry
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APA Style
Zuhair A-A. Khammas, Hawraa M. Abdulkareem. (2016). A New Visible Spectrophotometric Approach for Mutual Determination of Amoxicillin and Metoclopramide Hydrochloride in Pharmaceuticals After Cloud Point Extraction. Science Journal of Analytical Chemistry, 4(5), 66-76. https://doi.org/10.11648/j.sjac.20160405.12
ACS Style
Zuhair A-A. Khammas; Hawraa M. Abdulkareem. A New Visible Spectrophotometric Approach for Mutual Determination of Amoxicillin and Metoclopramide Hydrochloride in Pharmaceuticals After Cloud Point Extraction. Sci. J. Anal. Chem. 2016, 4(5), 66-76. doi: 10.11648/j.sjac.20160405.12
AMA Style
Zuhair A-A. Khammas, Hawraa M. Abdulkareem. A New Visible Spectrophotometric Approach for Mutual Determination of Amoxicillin and Metoclopramide Hydrochloride in Pharmaceuticals After Cloud Point Extraction. Sci J Anal Chem. 2016;4(5):66-76. doi: 10.11648/j.sjac.20160405.12
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Download@article{10.11648/j.sjac.20160405.12,
author = {Zuhair A-A. Khammas and Hawraa M. Abdulkareem},
title = {A New Visible Spectrophotometric Approach for Mutual Determination of Amoxicillin and Metoclopramide Hydrochloride in Pharmaceuticals After Cloud Point Extraction},
journal = {Science Journal of Analytical Chemistry},
volume = {4},
number = {5},
pages = {66-76},
doi = {10.11648/j.sjac.20160405.12},
url = {https://doi.org/10.11648/j.sjac.20160405.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20160405.12},
abstract = {A new approach has been developed and validated for the mutual determination of the drugs of amoxicillin (AMX) and Metoclopramide hydrochloride (MCP. HCl) in pharmaceuticals. The method is based on the reaction of diazotized Metoclopramide with amoxicillin in an alkaline medium to form an intense orange water-soluble product which can be easily extracted from micelles of a non-ionic surfactant (Triton X-114) and both drugs measured sequentially at the same absorption maximum of 479 nm. The optimization of all experimental variables was individually performed to obtain high extraction efficiency for both target medicaments. Under the optimized conditions, Beer’s law was obeyed in the concentration range of 0.3-3.0 μg mL-1 (r=0.9995) for both AMX and MCP. The enrichment factors were found to 214 and 90.85 fold for AMX and MCP, led to obtaining the detection limits of 0.083 and 0.098 μg mL5, and a superb sensitivity in terms of the molar absorptivity of 2.35x105 and 2.25x10-1 L.mol5.cm-1, respectively. The mean recovery percentage of 97.77±1.72% (in AMX capsule) and 98.20±1.95 (in MCP ampoule); the precision (RSD %) ranged between 2.35-10.8% and 0.20-3.43% were obtained for AMX and MCP respectively. The proposed method was validated and applied for determination of AMX and MCP in various samples of the pharmaceutical preparations.},
year = {2016}
}
TY - JOUR T1 - A New Visible Spectrophotometric Approach for Mutual Determination of Amoxicillin and Metoclopramide Hydrochloride in Pharmaceuticals After Cloud Point Extraction AU - Zuhair A-A. Khammas AU - Hawraa M. Abdulkareem Y1 - 2016/10/18 PY - 2016 N1 - https://doi.org/10.11648/j.sjac.20160405.12 DO - 10.11648/j.sjac.20160405.12 T2 - Science Journal of Analytical Chemistry JF - Science Journal of Analytical Chemistry JO - Science Journal of Analytical Chemistry SP - 66 EP - 76 PB - Science Publishing Group SN - 2376-8053 UR - https://doi.org/10.11648/j.sjac.20160405.12 AB - A new approach has been developed and validated for the mutual determination of the drugs of amoxicillin (AMX) and Metoclopramide hydrochloride (MCP. HCl) in pharmaceuticals. The method is based on the reaction of diazotized Metoclopramide with amoxicillin in an alkaline medium to form an intense orange water-soluble product which can be easily extracted from micelles of a non-ionic surfactant (Triton X-114) and both drugs measured sequentially at the same absorption maximum of 479 nm. The optimization of all experimental variables was individually performed to obtain high extraction efficiency for both target medicaments. Under the optimized conditions, Beer’s law was obeyed in the concentration range of 0.3-3.0 μg mL-1 (r=0.9995) for both AMX and MCP. The enrichment factors were found to 214 and 90.85 fold for AMX and MCP, led to obtaining the detection limits of 0.083 and 0.098 μg mL5, and a superb sensitivity in terms of the molar absorptivity of 2.35x105 and 2.25x10-1 L.mol5.cm-1, respectively. The mean recovery percentage of 97.77±1.72% (in AMX capsule) and 98.20±1.95 (in MCP ampoule); the precision (RSD %) ranged between 2.35-10.8% and 0.20-3.43% were obtained for AMX and MCP respectively. The proposed method was validated and applied for determination of AMX and MCP in various samples of the pharmaceutical preparations. VL - 4 IS - 5 ER -
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