Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb<sup>3+</sup> Ions in the Presence of Hexamine

Sharifah Ghaythan Dbsan Alharthi; Ayman Abdelaziz Osman; Salma Ali Altamimi; Fatma Elzahraa Ali

doi:doi:10.11648/j.sjac.20170501.11

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Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb³⁺ Ions in the Presence of Hexamine

Sharifah Ghaythan Dbsan Alharthi, Ayman Abdelaziz Osman, Salma Ali Altamimi, Fatma Elzahraa Ali

Published in Science Journal of Analytical Chemistry (Volume 5, Issue 1)

Received: 31 December 2016 Accepted: 12 January 2017 Published: 6 February 2017

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Abstract

A simple and highly sensitive method for the determination of Gemifloxacin Mesylate in bulk drug and pharmaceutical formulations. The method is based on the reaction of Gemifloxacin Mesylate with Tb³⁺ in the presence of hexamine at pH 7.2. The fluorescence was measured at 545 nm after excitation at 271 nm. The calibration graph was linear over the range 0.05–1.3 µg mL, with minimum quantification limit (LOQ) of 0.024 µg mL and minimum limit of detection (LOD) of 7.65×10^-3 µg mL. The proposed method was successfully applied for the determination of this drug in its dosage forms.

Published in	Science Journal of Analytical Chemistry (Volume 5, Issue 1)
DOI	10.11648/j.sjac.20170501.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Gemifloxacin Mesylate, Spectrofluorometry, Terbium(III), Hexamine, Ternary Complex

References

[1]	T. L. Lemke, D. A. Williams, V. F. Roche and S. W. Zito. (2008), Foye’s Principles of Medicinal Chemistry, 6th ed., Wolters Kluwer, Lippincott Williams & Wilkins, New York.
[2]	M. J. O’Neil, P. E. Heckelman, C. B. Koch, K. J. Roman, C. M. Kenny and M. R. D’Arecca. (2006). The Merck Index, An Encyclopedia of Chemicals, Drugs and Biologicals, 14th ed., Merck Research Laboratories Division of Merck & Co., Inc., Whitehouse Station (NJ).
[3]	B. A. Moussa, M. A. Mahrouse, M. A. Hassan and M. G. Fawzy. (2013). Stability indicating spectrophotometric and TLC densitometric methods for the determination of gemifloxacin mesylate in tablet form, Anal. Chem. Indian J. 12: 165–176.
[4]	C. S. Paim, F. Fuhr, M. Steppe, E. Schapoval and S. Eva. (2012) Gemifloxacin mesylate: UV spectrophotometric method for quantitative determination using experimental design for robustness, Quimica Nova. 35: 193–197.
[5]	R. El-Bagary, N. F. Abo-Talib and M. B. N. Eldin. (2011). Validated stability indicating assay of gemifloxacin by different chromatographic and spectrophotometric methods of analysis, J. Chem. Pharm. Res. 3: 562–570.
[6]	S. S. Panda, B. V. V. Ravi Kumar, K. S. Rao, V. R. Kumar and D. Patanaik. (2011). Difference spectrophotometric determination of gemifloxacin mesylate in tablet formulation, Asian J. Biochem. Pharm. Res. 1: 442–447.
[7]	K. B. C. Sekhar, D. Madhuri and N. Devanna. (2010). Direct and derivative spectrophotometric determination of gemifloxacin mesylate via metal chelate, Acta Cienc. Indica, Ser. Chem. 36: 165– 171.
[8]	R. Rote Ambadas and S. P. Pingle. (2010). Validated UV-spectrophotometric methods for determination of gemifloxacin mesylate in pharmaceutical tablet dosage forms, E-J. Chem. 7: S344–S348.
[9]	D. Madhuri, K. B. Chandrasekhar, N. Devanna and G. Somasekhar. (2010) Direct and derivative spectrophotometric determination of gemifloxacin mesylate in pure form and pharmaceutical preparations using p acceptors, Int. J. Pharm. Sci. Res. 1:222–230.
[10]	Z. Y. Al Shoaibi and A. A. Gouda. (2010). Spectrophotometric methods for the determination of gemifloxacin mesylate in pure form and pharmaceutical formulations, Anal. Chem: Indian J. 9: 129–136.
[11]	M. V. Krishna and D. G. Sankar. (2008). Utility of s and p-acceptors for the spectrophotometric determination of gemifloxacin mesylate in pharmaceutical formulations, E-J. Chem. 5: 493–498.
[12]	S. E. K. Tekkeli and A. Önal. (2011). Spectrofluorimetric methods for the determination of gemifloxacin in tablets and spiked plasma samples, J. Fluoresc. 21: 1001–1007.
[13]	N. F. Youssef and L. I. Bebawy. (2006). Spectrofluorometric methods for the determination of gemifloxacin mesylate and cefamandole nafate in bulk powder and pharmaceutical preparations, Bull. Fac. Pharm. Cairo Univ. 44: 215–227.
[14]	A. A. Elbashir, B. A. Saad, S. M. Abdussalam, K. M. M. Al-Azzam and H. Y. Aboul-Enein. (2008). Validated stability indicating assay of gemifloxacin and lomefloxacin in tablet formulations by capillary electrophoresis, J. Liq. Chromatogr. Rel. Tech. 31: 1465–1477.
[15]	A. R. Rote and S. P. Pingle. (2009). Reversed phase-HPLC and HPTLC methods for determination of gemifloxacin mesylate in human plasma, J. Chromatogr. B 877: 3719–3723.
[16]	D. Nagavalli, G. Abirami and S. K. Kumar (2011). Validated HPLC method for the simultaneous estimation of gemifloxacin mesylate and ambroxol hydrochloride in bulk and tablet dosage form, J. Pharm. Res. 4:1701–1703.
[17]	A. K. Kovar and M. Abdel-Hamid, Arch. Pharm.,317 (1984) 246.
[18]	Caulcutt, R. and Boddy, R. (1983). "Statistics for analytical Chemists" Chapman and Hall, London.
[19]	http://www.rxlist.com/lexapro-drug.htm
[20]	International Conference on Harmonization. (1995). ICH Harmonised Tripartite Guideline, Q2 (R1), Validation of Analytical Procedures: Text and Methodology, Switzerland.

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Sharifah Ghaythan Dbsan Alharthi, Ayman Abdelaziz Osman, Salma Ali Altamimi, Fatma Elzahraa Ali. (2017). Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine. Science Journal of Analytical Chemistry, 5(1), 1-7. https://doi.org/10.11648/j.sjac.20170501.11

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ACS Style

Sharifah Ghaythan Dbsan Alharthi; Ayman Abdelaziz Osman; Salma Ali Altamimi; Fatma Elzahraa Ali. Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine. Sci. J. Anal. Chem. 2017, 5(1), 1-7. doi: 10.11648/j.sjac.20170501.11

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AMA Style

Sharifah Ghaythan Dbsan Alharthi, Ayman Abdelaziz Osman, Salma Ali Altamimi, Fatma Elzahraa Ali. Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine. Sci J Anal Chem. 2017;5(1):1-7. doi: 10.11648/j.sjac.20170501.11

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@article{10.11648/j.sjac.20170501.11,
  author = {Sharifah Ghaythan Dbsan Alharthi and Ayman Abdelaziz Osman and Salma Ali Altamimi and Fatma Elzahraa Ali},
  title = {Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine},
  journal = {Science Journal of Analytical Chemistry},
  volume = {5},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.sjac.20170501.11},
  url = {https://doi.org/10.11648/j.sjac.20170501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20170501.11},
  abstract = {A simple and highly sensitive method for the determination of Gemifloxacin Mesylate in bulk drug and pharmaceutical formulations. The method is based on the reaction of Gemifloxacin Mesylate with Tb3+ in the presence of hexamine at pH 7.2. The fluorescence was measured at 545 nm after excitation at 271 nm. The calibration graph was linear over the range 0.05–1.3 µg mL, with minimum quantification limit (LOQ) of 0.024 µg mL and minimum limit of detection (LOD) of 7.65×10-3 µg mL. The proposed method was successfully applied for the determination of this drug in its dosage forms.},
 year = {2017}
}

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TY  - JOUR
T1  - Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine
AU  - Sharifah Ghaythan Dbsan Alharthi
AU  - Ayman Abdelaziz Osman
AU  - Salma Ali Altamimi
AU  - Fatma Elzahraa Ali
Y1  - 2017/02/06
PY  - 2017
N1  - https://doi.org/10.11648/j.sjac.20170501.11
DO  - 10.11648/j.sjac.20170501.11
T2  - Science Journal of Analytical Chemistry
JF  - Science Journal of Analytical Chemistry
JO  - Science Journal of Analytical Chemistry
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2376-8053
UR  - https://doi.org/10.11648/j.sjac.20170501.11
AB  - A simple and highly sensitive method for the determination of Gemifloxacin Mesylate in bulk drug and pharmaceutical formulations. The method is based on the reaction of Gemifloxacin Mesylate with Tb3+ in the presence of hexamine at pH 7.2. The fluorescence was measured at 545 nm after excitation at 271 nm. The calibration graph was linear over the range 0.05–1.3 µg mL, with minimum quantification limit (LOQ) of 0.024 µg mL and minimum limit of detection (LOD) of 7.65×10-3 µg mL. The proposed method was successfully applied for the determination of this drug in its dosage forms.
VL  - 5
IS  - 1
ER  -

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Author Information

Sharifah Ghaythan Dbsan Alharthi

Department of Chemistry, College of Science and Home Economic, Bisha University, Bisha, Saudi Arabia
Ayman Abdelaziz Osman

Department of Chemistry, College of Science and Home Economic, Bisha University, Bisha, Saudi Arabia
Salma Ali Altamimi

Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia
Fatma Elzahraa Ali

Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia

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Plain Text BibTeX RIS

APA Style

Sharifah Ghaythan Dbsan Alharthi, Ayman Abdelaziz Osman, Salma Ali Altamimi, Fatma Elzahraa Ali. (2017). Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine. Science Journal of Analytical Chemistry, 5(1), 1-7. https://doi.org/10.11648/j.sjac.20170501.11

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ACS Style

Sharifah Ghaythan Dbsan Alharthi; Ayman Abdelaziz Osman; Salma Ali Altamimi; Fatma Elzahraa Ali. Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine. Sci. J. Anal. Chem. 2017, 5(1), 1-7. doi: 10.11648/j.sjac.20170501.11

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AMA Style

Sharifah Ghaythan Dbsan Alharthi, Ayman Abdelaziz Osman, Salma Ali Altamimi, Fatma Elzahraa Ali. Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine. Sci J Anal Chem. 2017;5(1):1-7. doi: 10.11648/j.sjac.20170501.11

Copy | Download

@article{10.11648/j.sjac.20170501.11,
  author = {Sharifah Ghaythan Dbsan Alharthi and Ayman Abdelaziz Osman and Salma Ali Altamimi and Fatma Elzahraa Ali},
  title = {Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine},
  journal = {Science Journal of Analytical Chemistry},
  volume = {5},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.sjac.20170501.11},
  url = {https://doi.org/10.11648/j.sjac.20170501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.sjac.20170501.11},
  abstract = {A simple and highly sensitive method for the determination of Gemifloxacin Mesylate in bulk drug and pharmaceutical formulations. The method is based on the reaction of Gemifloxacin Mesylate with Tb3+ in the presence of hexamine at pH 7.2. The fluorescence was measured at 545 nm after excitation at 271 nm. The calibration graph was linear over the range 0.05–1.3 µg mL, with minimum quantification limit (LOQ) of 0.024 µg mL and minimum limit of detection (LOD) of 7.65×10-3 µg mL. The proposed method was successfully applied for the determination of this drug in its dosage forms.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Fluorometric Method for the Determination of Gemifloxacin Mesylate in Bulk and Pharmaceutical Formulations Using Tb3+ Ions in the Presence of Hexamine
AU  - Sharifah Ghaythan Dbsan Alharthi
AU  - Ayman Abdelaziz Osman
AU  - Salma Ali Altamimi
AU  - Fatma Elzahraa Ali
Y1  - 2017/02/06
PY  - 2017
N1  - https://doi.org/10.11648/j.sjac.20170501.11
DO  - 10.11648/j.sjac.20170501.11
T2  - Science Journal of Analytical Chemistry
JF  - Science Journal of Analytical Chemistry
JO  - Science Journal of Analytical Chemistry
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2376-8053
UR  - https://doi.org/10.11648/j.sjac.20170501.11
AB  - A simple and highly sensitive method for the determination of Gemifloxacin Mesylate in bulk drug and pharmaceutical formulations. The method is based on the reaction of Gemifloxacin Mesylate with Tb3+ in the presence of hexamine at pH 7.2. The fluorescence was measured at 545 nm after excitation at 271 nm. The calibration graph was linear over the range 0.05–1.3 µg mL, with minimum quantification limit (LOQ) of 0.024 µg mL and minimum limit of detection (LOD) of 7.65×10-3 µg mL. The proposed method was successfully applied for the determination of this drug in its dosage forms.
VL  - 5
IS  - 1
ER  -

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