Association of Polymorphisms at <i>PRLR</i> Gene Loci with Litter Size in Haimen Goat

Dejun Ji; Guohu Cheng; Yongjun Li; Hao Zhang; Haiyan Guo; Miaoying Cai

doi:doi:10.11648/j.bmb.20160102.13

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Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat

Dejun Ji, Guohu Cheng, Yongjun Li, Hao Zhang, Haiyan Guo, Miaoying Cai

Published in Biochemistry and Molecular Biology (Volume 1, Issue 2)

Received: 29 July 2016 Accepted: 8 August 2016 Published: 29 August 2016

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Abstract

Haimen goat is an exclusive and relatively rare indigenous Chinese goat breed that can produce high quality Brush Hair specialized in making valuable writing brush. To enlarge quickly the population size of Haimen goat, certain genetic markers that can improve litter size are needed in the breeding process. To search for suitable genetic markers related to litter size, the polymorphisms at the PRLR gene loci were investigated by PCR and single-strand conformation polymorphisms (SSCP) in Haimen goat, and their associations with litter size were analyzed by genetic tools. Results showed that two alleles are found on PRLR gene Intron 1, with gene frequencies was 0.59 and 0.41, respectively; the heterozygosity (He), effective allele number (Ne) and polymorphic information content (PIC) in Haimen Goat based on Intron 1 was 0.4838, 1.9372 and 0.3668, respectively; Two alleles were found on PRLR gene Intron 2, and gene frequencies was 0.79 and 0.21, respectively. He, Ne and PIC in Haimen Goat on Intron 2 was 0.3318, 1.4966 and 0.2768, respectively. The genetic variation indicated that the Haimen goat population was moderately polymorphic. The least square analysis and multicomparison of PRLR gene loci and litter size demonstrated that the SNP (A35G) on Intron 2 of the PRLR gene affected extremely significantly on litter size, with CC as the advantageous genotype for the trait of litter size; while the SNP (T95C) on Intron 1 had no significantly effect on litter size in Haimen goat. The results would provide some evidence for SNP (A35G) on Intron 2 of the PRLR gene as a candidate marker for the trait of litter size in goat.

Published in	Biochemistry and Molecular Biology (Volume 1, Issue 2)
DOI	10.11648/j.bmb.20160102.13
Page(s)	23-26
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Goat, PRLR, Litter Size, Polymorphism, PCR-SSCP

References

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[3]	Van Rens B T, Evans G J, Van der Lende T. (2003) Components of litter size in gilts with different prolactin receptor genotypes. Theriogenology, 59 (3-4):915-926.
[4]	XU Ning-Ying, ZHANG Sheng-Qiao, PENG Shu-Hong. (2003) Investigation on the Distribution and Their Effects on Reproduction Traits of Three Major Genes in Jinhua Pigs. Acta Genetica Sinica, 30 (12): 1090-1096.
[5]	CHU Ming-xing, ZHANG Gen-xi, WANG Jin-yu, FANG Li, YE Su-cheng. (2008) Polymorphism of Prolactin Receptor Gene and Its Relationship with Litter Size of Some Goat Breeds. Journal of Agricultural Biotechnology. 16 (4):725-726.
[6]	ZHANG Gen-Xi, CHU Ming-Xing, WANG Jin-Yu, FANG Li, YE Su-Cheng. (2007) Polymorphism of exon10 of prolactin receptor gene and its relationship with prolificacy of Jining Grey goats. HEREDITAS, 29 (3): 329-336.
[7]	Mullenbach R, Lagoda PJ, Welter C (1989) An efficient salt chloroform extraction of DNA from blood and tissue. Trends Genetics, 5:391.
[8]	MU Yu-lian, CHU Ming-xing, SUN Shaohua, FANG Li, YE Sucheng. (2006) PCR-SSCP Analysis on Prolactin Receptor Gene in Sheep. Acta Veterinaria et Zootechnica Sinica, 37 (10): 956-960.
[9]	ChunLei Zhang, Yanhong Wang, Hong Chen, XianYong Lan, ChuZhao Lei. (2007) Enhance the efficiency of single-strand conformation polymorphism analysis by short polyacrylamide gel and modified silver staining. Anal Biochem, 365: 286–287.
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APA Style

Dejun Ji, Guohu Cheng, Yongjun Li, Hao Zhang, Haiyan Guo, et al. (2016). Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat. Biochemistry and Molecular Biology, 1(2), 23-26. https://doi.org/10.11648/j.bmb.20160102.13

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ACS Style

Dejun Ji; Guohu Cheng; Yongjun Li; Hao Zhang; Haiyan Guo, et al. Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat. Biochem. Mol. Biol. 2016, 1(2), 23-26. doi: 10.11648/j.bmb.20160102.13

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AMA Style

Dejun Ji, Guohu Cheng, Yongjun Li, Hao Zhang, Haiyan Guo, et al. Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat. Biochem Mol Biol. 2016;1(2):23-26. doi: 10.11648/j.bmb.20160102.13

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@article{10.11648/j.bmb.20160102.13,
  author = {Dejun Ji and Guohu Cheng and Yongjun Li and Hao Zhang and Haiyan Guo and Miaoying Cai},
  title = {Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat},
  journal = {Biochemistry and Molecular Biology},
  volume = {1},
  number = {2},
  pages = {23-26},
  doi = {10.11648/j.bmb.20160102.13},
  url = {https://doi.org/10.11648/j.bmb.20160102.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20160102.13},
  abstract = {Haimen goat is an exclusive and relatively rare indigenous Chinese goat breed that can produce high quality Brush Hair specialized in making valuable writing brush. To enlarge quickly the population size of Haimen goat, certain genetic markers that can improve litter size are needed in the breeding process. To search for suitable genetic markers related to litter size, the polymorphisms at the PRLR gene loci were investigated by PCR and single-strand conformation polymorphisms (SSCP) in Haimen goat, and their associations with litter size were analyzed by genetic tools. Results showed that two alleles are found on PRLR gene Intron 1, with gene frequencies was 0.59 and 0.41, respectively; the heterozygosity (He), effective allele number (Ne) and polymorphic information content (PIC) in Haimen Goat based on Intron 1 was 0.4838, 1.9372 and 0.3668, respectively; Two alleles were found on PRLR gene Intron 2, and gene frequencies was 0.79 and 0.21, respectively. He, Ne and PIC in Haimen Goat on Intron 2 was 0.3318, 1.4966 and 0.2768, respectively. The genetic variation indicated that the Haimen goat population was moderately polymorphic. The least square analysis and multicomparison of PRLR gene loci and litter size demonstrated that the SNP (A35G) on Intron 2 of the PRLR gene affected extremely significantly on litter size, with CC as the advantageous genotype for the trait of litter size; while the SNP (T95C) on Intron 1 had no significantly effect on litter size in Haimen goat. The results would provide some evidence for SNP (A35G) on Intron 2 of the PRLR gene as a candidate marker for the trait of litter size in goat.},
 year = {2016}
}

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TY  - JOUR
T1  - Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat
AU  - Dejun Ji
AU  - Guohu Cheng
AU  - Yongjun Li
AU  - Hao Zhang
AU  - Haiyan Guo
AU  - Miaoying Cai
Y1  - 2016/08/29
PY  - 2016
N1  - https://doi.org/10.11648/j.bmb.20160102.13
DO  - 10.11648/j.bmb.20160102.13
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 23
EP  - 26
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20160102.13
AB  - Haimen goat is an exclusive and relatively rare indigenous Chinese goat breed that can produce high quality Brush Hair specialized in making valuable writing brush. To enlarge quickly the population size of Haimen goat, certain genetic markers that can improve litter size are needed in the breeding process. To search for suitable genetic markers related to litter size, the polymorphisms at the PRLR gene loci were investigated by PCR and single-strand conformation polymorphisms (SSCP) in Haimen goat, and their associations with litter size were analyzed by genetic tools. Results showed that two alleles are found on PRLR gene Intron 1, with gene frequencies was 0.59 and 0.41, respectively; the heterozygosity (He), effective allele number (Ne) and polymorphic information content (PIC) in Haimen Goat based on Intron 1 was 0.4838, 1.9372 and 0.3668, respectively; Two alleles were found on PRLR gene Intron 2, and gene frequencies was 0.79 and 0.21, respectively. He, Ne and PIC in Haimen Goat on Intron 2 was 0.3318, 1.4966 and 0.2768, respectively. The genetic variation indicated that the Haimen goat population was moderately polymorphic. The least square analysis and multicomparison of PRLR gene loci and litter size demonstrated that the SNP (A35G) on Intron 2 of the PRLR gene affected extremely significantly on litter size, with CC as the advantageous genotype for the trait of litter size; while the SNP (T95C) on Intron 1 had no significantly effect on litter size in Haimen goat. The results would provide some evidence for SNP (A35G) on Intron 2 of the PRLR gene as a candidate marker for the trait of litter size in goat.
VL  - 1
IS  - 2
ER  -

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Author Information

Dejun Ji

Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China
Guohu Cheng

Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China
Yongjun Li

Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China
Hao Zhang

Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China
Haiyan Guo

Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China
Miaoying Cai

Key Laboratory for Animal Genetics & Molecular Breeding of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou, China

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Plain Text BibTeX RIS

APA Style

Dejun Ji, Guohu Cheng, Yongjun Li, Hao Zhang, Haiyan Guo, et al. (2016). Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat. Biochemistry and Molecular Biology, 1(2), 23-26. https://doi.org/10.11648/j.bmb.20160102.13

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ACS Style

Dejun Ji; Guohu Cheng; Yongjun Li; Hao Zhang; Haiyan Guo, et al. Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat. Biochem. Mol. Biol. 2016, 1(2), 23-26. doi: 10.11648/j.bmb.20160102.13

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AMA Style

Dejun Ji, Guohu Cheng, Yongjun Li, Hao Zhang, Haiyan Guo, et al. Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat. Biochem Mol Biol. 2016;1(2):23-26. doi: 10.11648/j.bmb.20160102.13

Copy | Download

@article{10.11648/j.bmb.20160102.13,
  author = {Dejun Ji and Guohu Cheng and Yongjun Li and Hao Zhang and Haiyan Guo and Miaoying Cai},
  title = {Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat},
  journal = {Biochemistry and Molecular Biology},
  volume = {1},
  number = {2},
  pages = {23-26},
  doi = {10.11648/j.bmb.20160102.13},
  url = {https://doi.org/10.11648/j.bmb.20160102.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.bmb.20160102.13},
  abstract = {Haimen goat is an exclusive and relatively rare indigenous Chinese goat breed that can produce high quality Brush Hair specialized in making valuable writing brush. To enlarge quickly the population size of Haimen goat, certain genetic markers that can improve litter size are needed in the breeding process. To search for suitable genetic markers related to litter size, the polymorphisms at the PRLR gene loci were investigated by PCR and single-strand conformation polymorphisms (SSCP) in Haimen goat, and their associations with litter size were analyzed by genetic tools. Results showed that two alleles are found on PRLR gene Intron 1, with gene frequencies was 0.59 and 0.41, respectively; the heterozygosity (He), effective allele number (Ne) and polymorphic information content (PIC) in Haimen Goat based on Intron 1 was 0.4838, 1.9372 and 0.3668, respectively; Two alleles were found on PRLR gene Intron 2, and gene frequencies was 0.79 and 0.21, respectively. He, Ne and PIC in Haimen Goat on Intron 2 was 0.3318, 1.4966 and 0.2768, respectively. The genetic variation indicated that the Haimen goat population was moderately polymorphic. The least square analysis and multicomparison of PRLR gene loci and litter size demonstrated that the SNP (A35G) on Intron 2 of the PRLR gene affected extremely significantly on litter size, with CC as the advantageous genotype for the trait of litter size; while the SNP (T95C) on Intron 1 had no significantly effect on litter size in Haimen goat. The results would provide some evidence for SNP (A35G) on Intron 2 of the PRLR gene as a candidate marker for the trait of litter size in goat.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Association of Polymorphisms at PRLR Gene Loci with Litter Size in Haimen Goat
AU  - Dejun Ji
AU  - Guohu Cheng
AU  - Yongjun Li
AU  - Hao Zhang
AU  - Haiyan Guo
AU  - Miaoying Cai
Y1  - 2016/08/29
PY  - 2016
N1  - https://doi.org/10.11648/j.bmb.20160102.13
DO  - 10.11648/j.bmb.20160102.13
T2  - Biochemistry and Molecular Biology
JF  - Biochemistry and Molecular Biology
JO  - Biochemistry and Molecular Biology
SP  - 23
EP  - 26
PB  - Science Publishing Group
SN  - 2575-5048
UR  - https://doi.org/10.11648/j.bmb.20160102.13
AB  - Haimen goat is an exclusive and relatively rare indigenous Chinese goat breed that can produce high quality Brush Hair specialized in making valuable writing brush. To enlarge quickly the population size of Haimen goat, certain genetic markers that can improve litter size are needed in the breeding process. To search for suitable genetic markers related to litter size, the polymorphisms at the PRLR gene loci were investigated by PCR and single-strand conformation polymorphisms (SSCP) in Haimen goat, and their associations with litter size were analyzed by genetic tools. Results showed that two alleles are found on PRLR gene Intron 1, with gene frequencies was 0.59 and 0.41, respectively; the heterozygosity (He), effective allele number (Ne) and polymorphic information content (PIC) in Haimen Goat based on Intron 1 was 0.4838, 1.9372 and 0.3668, respectively; Two alleles were found on PRLR gene Intron 2, and gene frequencies was 0.79 and 0.21, respectively. He, Ne and PIC in Haimen Goat on Intron 2 was 0.3318, 1.4966 and 0.2768, respectively. The genetic variation indicated that the Haimen goat population was moderately polymorphic. The least square analysis and multicomparison of PRLR gene loci and litter size demonstrated that the SNP (A35G) on Intron 2 of the PRLR gene affected extremely significantly on litter size, with CC as the advantageous genotype for the trait of litter size; while the SNP (T95C) on Intron 1 had no significantly effect on litter size in Haimen goat. The results would provide some evidence for SNP (A35G) on Intron 2 of the PRLR gene as a candidate marker for the trait of litter size in goat.
VL  - 1
IS  - 2
ER  -

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