Review on Diagnostic Techniques of Bovine Tuberculosis
International Journal of Biochemistry, Biophysics & Molecular Biology
Volume 1, Issue 1, October 2016, Pages: 11-17
Received: Jun. 2, 2016; Accepted: Jun. 30, 2016; Published: Oct. 9, 2016
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Akinaw Wagari, School of Veterinary Medicine, Wollega University, Nekemte, Ethiopia
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Bovine tuberculosis is a major infectious disease of cattle, other domesticated animals and certain wildlife populations. It is one of the zoonotic diseases and can be diagnosed with different techniques. The currently used techniques are acid fast staining which is a cost-effective tool for diagnosing tuberculosis case and to monitor the progress of treatment and it has also drawbacks such as the low sensitivity in the immune-suppressed individuals; After death, infection is diagnosed by necropsy, histopathological and bacteriological examination and biochemical tests like nitrate reduction, niacin production, deamination of pyrazinamide and urease tests. Immunological diagnostic techniques like tuberculin skin tests: Single intradermal test, comparative intradermal test, short thermal test and stormont test; Blood based diagnostic techniques like gamma interferon assays, Enzyme-linked immune-sorbent assays and lymphocyte proliferation assay, culture of mycobacterium and molecular diagnostic techniques which involves polymerase chain reaction, is a method that allows direct identification of the M. tuberculosis complex. Spoligotyping, restriction fragment length polymorphism, variable number tandem repeats typing are the techniques used for concurrent detection and typing of mycobacterium species at strain level. Its clinical usefulness over the other techniques is determined by its rapidity, both in identifying causative bacteria and in providing molecular epidemiologic information on strains. However, it holds drawbacks of being expensive and requiring well-equipped laboratory and skilled laboratory personnel, which are not always available in endemic areas. Thus, both conventional and molecular tools should be effectively used to diagnosis Tuberculosis.
Bovine Tuberculosis, Cattle, Diagnostic Techniques
To cite this article
Akinaw Wagari, Review on Diagnostic Techniques of Bovine Tuberculosis, International Journal of Biochemistry, Biophysics & Molecular Biology. Vol. 1, No. 1, 2016, pp. 11-17. doi: 10.11648/j.ijbbmb.20160101.12
Copyright © 2016 Authors retain the copyright of this article.
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