American Journal of Clinical and Experimental Medicine

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Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss

Received: 21 April 2016    Accepted: 29 April 2016    Published: 11 May 2016
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Abstract

In the absence of confirmed causes for around 50% of recurrent pregnancy loss (RPL) cases this study was conducted in order to investigate the association between single nucleotide polymorphism (SNP) in regulatory T-cell related STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF (rs3753082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) genes and unexplained RPL in a group of Palestinian women residing in Gaza strip. A retrospective case-control study was carried out during the period (August 2015 to March 2016). A total of 200 females, 100 RPL patients and 100 control women without previous history of RPL, aged 20–35 years were included in the study. STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF (rs375082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) polymorphisms were tested by PCR-RFLP. Statistically significant difference existed between RPL cases and controls in terms of the genotypic distribution of the tested polymorphisms. STAT3 CC, FOXP3 AA, LIF CC, NKG7 AA and CCR5 GG genotypes were significantly higher in the RPL group. The tested polymorphisms shape the first elements of immune tolerance-related risk SNPs panel for RPL in the investigated population and may lead to improved therapeutic approaches.

DOI 10.11648/j.ajcem.20160403.15
Published in American Journal of Clinical and Experimental Medicine (Volume 4, Issue 3, May 2016)
Page(s) 63-67
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Regulatory T-cells, STAT3, FOXP3, LIF, NKG7, CCR5, Polymorphism, Recurrent Pregnancy Loss

References
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Author Information
  • Department of Medical Laboratory Sciences, Islamic University of Gaza, Gaza, Palestine

  • Department of Medical Laboratory Sciences, Islamic University of Gaza, Gaza, Palestine

  • Department of Medical Laboratory Sciences, Islamic University of Gaza, Gaza, Palestine

  • Department of Medical Laboratory Sciences, Islamic University of Gaza, Gaza, Palestine

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  • APA Style

    Fadel A. Sharif, Mohammed J. Ashour, Naim T. Badawi, Shadi F. Al-Ashi. (2016). Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss. American Journal of Clinical and Experimental Medicine, 4(3), 63-67. https://doi.org/10.11648/j.ajcem.20160403.15

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    ACS Style

    Fadel A. Sharif; Mohammed J. Ashour; Naim T. Badawi; Shadi F. Al-Ashi. Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss. Am. J. Clin. Exp. Med. 2016, 4(3), 63-67. doi: 10.11648/j.ajcem.20160403.15

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    AMA Style

    Fadel A. Sharif, Mohammed J. Ashour, Naim T. Badawi, Shadi F. Al-Ashi. Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss. Am J Clin Exp Med. 2016;4(3):63-67. doi: 10.11648/j.ajcem.20160403.15

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  • @article{10.11648/j.ajcem.20160403.15,
      author = {Fadel A. Sharif and Mohammed J. Ashour and Naim T. Badawi and Shadi F. Al-Ashi},
      title = {Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss},
      journal = {American Journal of Clinical and Experimental Medicine},
      volume = {4},
      number = {3},
      pages = {63-67},
      doi = {10.11648/j.ajcem.20160403.15},
      url = {https://doi.org/10.11648/j.ajcem.20160403.15},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ajcem.20160403.15},
      abstract = {In the absence of confirmed causes for around 50% of recurrent pregnancy loss (RPL) cases this study was conducted in order to investigate the association between single nucleotide polymorphism (SNP) in regulatory T-cell related STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF  (rs3753082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) genes and unexplained RPL in a group of Palestinian women residing in Gaza strip. A retrospective case-control study was carried out during the period (August 2015 to March 2016). A total of 200 females, 100 RPL patients and 100 control women without previous history of RPL, aged 20–35 years were included in the study. STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF  (rs375082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) polymorphisms were tested by PCR-RFLP. Statistically significant difference existed between RPL cases and controls in terms of the genotypic distribution of the tested polymorphisms. STAT3 CC, FOXP3 AA, LIF  CC, NKG7 AA and CCR5 GG genotypes were significantly higher in the RPL group. The tested polymorphisms shape the first elements of immune tolerance-related risk SNPs panel for RPL in the investigated population and may lead to improved therapeutic approaches.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Polymorphism in Regulatory T-cell (Treg)-Related Genes Is Associated with Unexplained Recurrent Pregnancy Loss
    AU  - Fadel A. Sharif
    AU  - Mohammed J. Ashour
    AU  - Naim T. Badawi
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    JF  - American Journal of Clinical and Experimental Medicine
    JO  - American Journal of Clinical and Experimental Medicine
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    EP  - 67
    PB  - Science Publishing Group
    SN  - 2330-8133
    UR  - https://doi.org/10.11648/j.ajcem.20160403.15
    AB  - In the absence of confirmed causes for around 50% of recurrent pregnancy loss (RPL) cases this study was conducted in order to investigate the association between single nucleotide polymorphism (SNP) in regulatory T-cell related STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF  (rs3753082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) genes and unexplained RPL in a group of Palestinian women residing in Gaza strip. A retrospective case-control study was carried out during the period (August 2015 to March 2016). A total of 200 females, 100 RPL patients and 100 control women without previous history of RPL, aged 20–35 years were included in the study. STAT3 (rs4796793 C/G), FOXP3 (rs3761548 A/C), LIF  (rs375082 T/C), NKG7 (rs71358833 A/G) and CCR5 (rs34418657 G/T) polymorphisms were tested by PCR-RFLP. Statistically significant difference existed between RPL cases and controls in terms of the genotypic distribution of the tested polymorphisms. STAT3 CC, FOXP3 AA, LIF  CC, NKG7 AA and CCR5 GG genotypes were significantly higher in the RPL group. The tested polymorphisms shape the first elements of immune tolerance-related risk SNPs panel for RPL in the investigated population and may lead to improved therapeutic approaches.
    VL  - 4
    IS  - 3
    ER  - 

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