Prevalence and Antibiotic Susceptibility Pattern of Streptococcus agalactiae Among Pregnant Women at Adigrat Zonal Hospital and Adigrat Health Center, Tigray, Ethiopia
Journal of Gynecology and Obstetrics
Volume 3, Issue 2, March 2015, Pages: 29-35
Received: Feb. 6, 2015; Accepted: Feb. 19, 2015; Published: Mar. 2, 2015
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Authors
Tsega Kahsay Gebremeskel, Adigrat University, College of Medicine and Health Sciences, Department of Bio Medical Science, Adigrat, Tigray, Ethiopia
Tamrat Abebe Zeleke, Addis Abeba University, College of Health Sciences, Department of Microbiology, Immunology and Parasitology, Addis Abeba, Ethiopia
Adane Mihret, Addis Abeba University, College of Health Sciences, Department of Microbiology, Immunology and Parasitology, Addis Abeba, Ethiopia
Mulugeta Desta Tikue, Adigrat Zonal Hospital, Gynecologist, Adigrat, Tigray, Ethiopia
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Abstract
Back ground: Streptococcus agalactiae or Lancefield group B Streptococci (GBS) is part of the microbiota of the mucous membranes of humans and animals, mainly colonizing the intestinal and genitourinary tracts. This study was undertaken to determine the carriage rate of S. agalactiae and to assess their antimicrobial susceptibility pattern. An attempt has been also made to identify the possible risk factors related with S. agalactiae colonization. Methods: Rectal and vaginal swabs were obtained from 150 pregnant women at 35-37 weeks of gestational period that attended antenatal clinic at Adigrat Zonal Hospital and Adigrat Health Center, Tigray, Ethiopia. Then the specimen was cultured on selective CHROMagarTMStrepB. In cases of positive cultures obtained, antibiotic susceptibility tests were carried out on all S. agalactiae isolates using the disc diffusion technique on Mueller-Hinton agar supplemented with 5% sheep blood. A univariate and multivariate binary logistic regression model was used to ascertain the association between the frequencies of colonization in relation to the different variables. Results: The prevalence of rectovaginal S. agalactiae colonization was (11.3%). Bacterial resistance was detected to erythromycin (11.8%) and clindamycin (17.6%). By multi variant logistic regression analysis, prolonged rupture of membrane was associated with a higher colonization rate of S. agalactiae (OR=5.864, 95% CI= 1.395 – 24.643, P-value= 0.016). Conclusion: The carriage rate of S. agalactiae among pregnant women in the present and a previous study in Gondar, remain low. The rates, risk factors of maternal GBS colonization may vary in different communities and need to be thoroughly evaluated in each country to allow the most appropriate preventive strategy to be selected.
Keywords
Prevalence, S. agalactiae, Pregnancy, Colonization, Ethiopia
To cite this article
Tsega Kahsay Gebremeskel, Tamrat Abebe Zeleke, Adane Mihret, Mulugeta Desta Tikue, Prevalence and Antibiotic Susceptibility Pattern of Streptococcus agalactiae Among Pregnant Women at Adigrat Zonal Hospital and Adigrat Health Center, Tigray, Ethiopia, Journal of Gynecology and Obstetrics. Vol. 3, No. 2, 2015, pp. 29-35. doi: 10.11648/j.jgo.20150302.13
References
[1]
Centers for Disease Control: Prevention of perinatal group B Streptococcal disease: a public health perspective. MMWR Recomm Rep 1996, 45(RR-7):1-24.
[2]
Schuchat A: Prevention effectiveness case study: institutionalizing prevention of group B Streptococcal infections national center for infectious diseases centers for disease control and prevention Atlanta, Georgia 1995, 227-32.
[3]
Rollins DM: Streptococcus summary. [http://life.umd.edu/classroom/bsci424].
[4]
Murray PR, Rosenthal KS and Pfaller MA: Medical microbiology. (5thed) USA: 2005: 247-250.
[5]
Bergseng H, Bevanger L, Rygg M, Bergh K: Real-time PCR targeting the sip gene for detection of group B Streptococcus colonization in pregnant women at delivery. J Med Microbiol 2007, 56(pt 2):223–8.
[6]
Knox JM: Group B Streptococcal infection: a review and update. Br J Vener Dis 1979, 55(2):118-20.
[7]
Tor-Udom S, Tor-Udom P, Hiriote W: The prevalence of Streptococcus agalactiae (Group B) colonization in pregnant women at Thammasat Hospital. J Med Assoc Thai 2006, 89(4):411-4.
[8]
Chua S, Arulkumaran S, Chow C, Kumarasinghe G, Selamat N, Kuah BG, Ratnam SS: Genital group B Streptococcus carriage in the antenatal period: its role in PROM and preterm labor. Singapore MED J 1995, 36(4):383-5.
[9]
Church DL, Baxter H, Lioyd T, Miller B, Elsayed S: Evaluation of StrepB carrot broth versus lim broth for detection of group B Streptococcus colonization status of near-term pregnant women. J Clin Microbiol 2008, 46(8):2780–2.
[10]
Stoll BJ, Schuchat A: Maternal carriage of group B Streptococci in developing countries. Pediatr Infect Dis J 1998, 17(6):499-503.
[11]
Schmidt J, Halle E, Halle H, Mohammed T, Gunther E: Colonization of pregnant women and their newborn infants with group B Streptococci in the Gondar College of medical sciences. Ethiop Med J 1989, 27(3):115-9.
[12]
World Health Organization: Country Cooperation Strategy 2008–2011.
[13]
Murayama SY, Seki C, Sakata H, Sunaoshi K, Nakayama E, Iwata S, Sunakawa K, Ubukata K; Invasive Streptococcal Disease Working Group. Capsular Type and Antibiotic Resistance in Streptococcus agalactiae Isolates from Patients, Ranging from Newborns to the Elderly, with Invasive Infections. Antimicrobial Agents and Chemotherapy 2009, 53(6): 2650-2653.
[14]
Orrett FA: Colonization with Group B streptococci in pregnancy and outcome of infected neonates in Trinidad. Pediatr Int 2003, 45:319–323.
[15]
Kouji K, Noriyuki N, Yukiko N, Jun-ichi W, Satowa S, Keigo S, Yoshichika A: Predominance of sequence type 1 group with serotype VI among group B streptococci with reduced penicillin susceptibility. J Antimicrob Chemother 2011, 66(11): 2460-2464.
[16]
Noriyuki N, Kouji K, Yukiko N, Hiroaki Y, Yoshichika A. Molecular characterization of group B streptococci with reduced penicillin susceptibility recurrently isolated from a sacral decubitus ulcer. J Antimicrob Chemother 2009, 64(6):1326-1328.
[17]
Abdelmoaty T, Wafaa Z, Kawthar M: Prevalence and antibiotic susceptibility of anogenital group B streptococci colonization in pregnant women. Egypt J Med Lab 2009, 18(2):105-111.
[18]
Joachim A, Matee MI, Massawe FA, Lyamuya EF: Maternal and neonatal colonization of group B Streptococcus at Muhimbili national hospital in Dares Salaam, Tanzania: prevalence, risk factors and antimicrobial resistance. BMC Public Health 2009, 9:437.
[19]
De Mouy D, Cavallo JD, Leclercq R, Fabre R: Antibiotic susceptibility and mechanisms of erythromycin resistance in clinical isolates of Streptococcus agalactiae. Antimicrob Agents Chemother 2001, 45(8):2400–2.
[20]
Quiroga M, Pegels E, Oviedo P, Pereyra E, Vergara M: Antibiotic susceptibility patterns and Prevalence of Group B Streptococcus Isolated from Pregnant women in Misiones, Argentina. Brazilian Journal of Microbiology 2008, 39: 245-250.
[21]
Motlova J, Strakova L, Urbaskova P, Sak P, Sever T: Vaginal and rectal carriage of Streptococcus agalactiae in the Czech Republic: incidence, serotypes distribution and susceptibility to antibiotics. Indian J Med Res 2004, 119 (Suppl):84-7.
[22]
Manning SD, Schaeffer KE, Springman AC, Lehotzky E, Lewis MA, Ouellette LM, Wu G, Moorer GM, Whittam TS, Davies HD: Genetic diversity and antimicrobial resistance in group B Streptococcus colonizing young, non-pregnant women. Clin Infect Dis 2008, 47(3):388–90.
[23]
Centers for Disease Control and Prevention: Prevention of perinatal group B Streptococcal disease revised guidelines from CDC, 2010. MMWR Recomm Rep 2010, 59 (RR-10):1-36.
[24]
Clinical and Laboratory Standards Institute: Performance standards for antimicrobial susceptibility testing; Twentieth informational supplement.M100-S20 2010, 30(1).
[25]
Uduman SA, ChatterjeeTK, Al-Mouzan MI, AL-Suleiman S: Group B Streptococci colonization among Saudi women in labor and neonatal acquisition. Int J Gynaecol Obstet 1985, 23(1):21-4.
[26]
Nahaei MR, Ghandchilar N, Bilan N, Ghahramani P: Maternal carriage and neonatal colonization of Streptococcus agalactiae in Tabriz, Northwest Iran. Iran J Med Sci2007, 32(3):177-81.
[27]
Collins TS, Calderon M, Gilman RH, Vivar A, Charache P: Group B Streptococcal colonization in a developing country: it‘s association with sexually transmitted disease and socioeconomic factors. Am J Trop Med Hyg 1998, 59(4):633–6.
[28]
Andrews JI, Diekema DJ, Hunter SK, Rhomberg PR, Pfaller MA, Jones RN, Doern GV: Group B Streptococcicausing neonatal bloodstream infection: antimicrobial susceptibility and serotyping results from sentry centers in the western hemisphere. Am J Obstet Gynecol 2000, 183(4):859-62.
[29]
Dechen TC, Sumit K, Ranabir P: Correlates of vaginal colonization with group B Streptococci among pregnant women. J Glob Infect Dis 2010, 2(3):236-41.
[30]
Sharmila V, Joseph NM, ArunBabu T, Chaturvedula L, Sistla S: Genital tract group B Streptococcal colonization in pregnant women: a South Indian perspective. J Infect DevCtries 2011, 5(8):592-5.
[31]
Orrett FA, Olagundoye V: Prevalence of group B Streptococcal colonization in pregnant third trimester women in Trinidad. J Hosp Infect 1994, 27(1):43-8.
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