Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography

Doudou Zhang; Beijiao Cui; Xiaoya Jiang; Ligai Bai; Haiyan Liu

doi:doi:10.11648/j.ajpst.20160202.11

| Peer-Reviewed

Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography

Doudou Zhang, Beijiao Cui, Xiaoya Jiang, Ligai Bai, Haiyan Liu

Published in American Journal of Polymer Science and Technology (Volume 2, Issue 2)

Received: 27 August 2016 Accepted: 23 September 2016 Published: 14 October 2016

Views: Downloads:

Download PDF

Share This Article

Twitter
Linked In
Facebook

Abstract

In the present work, polymer monolithic columns were prepared via atom transfer radical polymerization technique by using triallyl isocyanurate as monomer, ethylene glycol dimethacrylate as cross linking agent, polyethyleneglycol 200 and 1,4-butylene glycol as binary porogens, N,N-dimethylformamide as solvent, FeCl₂ as catalyst, and CCl₄ as initiator. Different monolithic columns were obtained by changing the conditions or columns sizes. These columns were used as the stationary phases of high performance liquid chromatography to investigate the factor effecting on the column efficiency. The effects of column diameter (D), pore size (d), porosity (Ö), and linear velocity of the mobile phase (u) on the performance of polymer monolithic columns were studied and the results certified that the plug-like flow in the column was one of the key factors shaping high performance. Furthermore, a structural hydrodynamic model was introduced to investigate the effects of polymer morphology on the performance of liquid chromatography with monolithic columns.

Published in	American Journal of Polymer Science and Technology (Volume 2, Issue 2)
DOI	10.11648/j.ajpst.20160202.11
Page(s)	20-27
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Polymer Monolithic Column, Column Efficiency, High Performance Liquid Chromatography, Structural Flow, Atom Transfer Radical Polymerization

References

[1]	J. H. Knox, “Effect of skeleton size on the performance of octadecylsilylated continuous porous silica columns in reversed-phase liquid chromatography,” J. Chromatogr. Sci. vol. 15, pp. 352-364, 1977.
[2]	G. J. Kennedy, and J. H. J. Knox, “Capacity of sampling and preconcentration columnswith a low number of theoretical plates,” Chromatogr. Sci. vol. 10, pp. 549-556, 1972.
[3]	J. H. Knox, and J. F. Parcher, “Effect of the column to particle diameter ratio on the dispersion of unsorbed solutes in chromatography,” Anal. Chem. Vol. 41, pp. 1599-1606, 1969.
[4]	T. Hara, H. Kobayashi, and T. Ikegami, “Performance of monolithic silica capillary columns with increased phase ratios and small-sized domains,” Anal. Chem. vol. 78, pp. 7632-7642, 2006.
[5]	Y. Ueki, T. Umemura, and J. Li, “Preparation and application of methacrylate-based cation-exchange monolithic columns for capillary ion chromatography,” Anal. Chem. vol. 76, pp. 7007-7012, 2004.
[6]	M. Wu, R. Wu, and R. Li, “Polyhedral oligomeric silsesquioxane as a cross-linker for preparation of inorganic−organic hybrid monolithic columns,” Anal. Chem. vol. 82, pp. 5447-5454, 2010.
[7]	B. Gu, Z. Chen, and C. D. “Thulin, efficient polymer monolith for strong cation-exchange capillary liquid chromatography of peptides,” Anal. Chem. vol. 78. pp. 3509-3518, 2006.
[8]	X. J. Zhou, and Q. B. Guo, “Construction of a fusion bacterial strain and the profile control characteristic of it,” Chin. J. Xian Shiyou Univer. vol. 22, pp. 37-44, 2007.
[9]	H. Chate, E. Villermaux, and J. M. Chomaz, “In mixing: chaos and turbulence, ”Springer, New York, 1999.
[10]	B. V. Antohe, and J. L. Lage, “A general two-equation macroscopic turbulence model for incompressible flow in porous media,” J. Heat Mass Transfer vol. 40, pp. 3013-3024, 1997.
[11]	J. Bear, “Dynamics of fluids in porous media,” Elsevier: New York, 1972.
[12]	G. Schneebeli, HouilleBlanche, vol. 10, pp. 141-149, 1955.
[13]	D. E. Wright, and CivilEng. Hydraul. Div. “Nonlinear flow through granular media,” Proc. Am. Soc. vol. 94, pp. 851-872, 1968.
[14]	J. Comiti, N. E. Sabiri, and A. Montillet, “Experimental characterization of flow regimes in various porous media — III: limit of Darcy's or creeping flow regime for Newtonian and purely viscous non-Newtonian fluids,” Chem. Eng. Sci. vol. 55, pp. 3057-3061, 2000.
[15]	C. R. Dudgeon, “An experimental study of the flow of water through coarse granular media,” Houille Blanche. vol. 21, pp. 785-801, 1966.
[16]	G. Chauveteau, and E. L. Darcy, Doctoral Thesis, University of Toulouse, 1965.
[17]	J. Bear, “Dynamics of Fluids in Porous Media,” Dover Publications, NewYork, 1988, pp. 181
[18]	H. Zhang, L. Bai, Z. Wei, S. Liu, H. Liu, and H. Yan, “Fabrication of an ionic liquid-based macroporous polymer monolithic column via atom transfer radical polymerization for the separation of small molecules,” Talanta vol. 149, pp. 62-68, 2016.
[19]	J. Zhong, M. Hao, R. Li, L. Bai, and G. Yang, “Preparation and characterization of poly (triallyl isocyanurate -co- trimethylolpropane triacrylate) monolith and its applications in the separation of small molecules by liquid chromatography,” J. Chromatogr. A vol 1333, pp. 79-86, 2014.
[20]	L. P. Franca, and S. L. Frey, “Stabilized finite element methods: II. The incompressible Navier-Stokes equations,” Comput. Meth. Appl. Mech. Eng. vol. 99, pp. 209–233, 1992.
[21]	E. X. Yuan, “Engineering Fluid Mechanics,” Petroleum Industry Press: Beijing, 1986.
[22]	C. T. Hsu, and P. Cheng, Int. Thermal dispersion in a porous medium, J. Heat Mass Transfer. vol. 33, 1990, pp. 1587-1597.
[23]	K. Hosoya, N. Hira, and K. Yamamoto, “High-performance polymer-based monolithic capillary column,” Anal. Chem. vol. 78, pp. 5729-5735, 2006.
[24]	M. Q. Liu, H. Y. Liu, and Y. K. Liu, “Preparation and characterization of temperature-responsive poly (N-isopropylacrylamide-co-N,N′-Methylenebisacrylamide) monolith for HPLC,” J. Chromatogr. A. vol. 1218, pp. 286-292, 2011.
[25]	Q. Wang, F. Svec, and J. M. J. Fréchet, “Reversed-phase chromatography of small molecules and peptides on a continuous rod of macroporous poly (styrene-co-divinylbenzene),” J. Chromatogr. A. vol. 669, pp. 230-235, 1994.
[26]	P. Coufal, M. Cihak, and J. Suchankova, “Methacrylate monolithic columns of 320 μm I. D. for capillary liquid chromatography, ” J. Chromatogr. A. vol. 946, pp. 99-106, 2002.
[27]	J. Lin, J. Lin, X. C. Lin, and Z. H. Xie, “Capillary liquid chromatography using a hydrophilic/cation-exchange monolithic column with a dynamically modified cationic surfactant,” J. Chromatogr. A. vol. 1216, pp. 7728–7731, 2009.
[28]	J. Urban, F. Svec, and J. M. J. Fréchet, “Hypercrosslinking: New approach to porous polymer monolithic capillary columns with large surface area for the highly efficient separation of small molecules, ” J. Chromatogr. A. vol. 1217, pp. 8212–8221, 2010.
[29]	Y. Y. Li. H. Dennis Tolley, and M. L. Lee, “Preparation of monoliths from single crosslinking monomers for reversed-phase capillary chromatography of small molecules, ” J. Chromatogr. A. vol. 1218, pp. 1399–1408, 2011.
[30]	Y. Huo, P. J. Schoenmakers, and W. T. Kok, “Efficiency of methacrylate monolithic columns in reversed-phase liquid chromatographic separations,” J. Chromatogr. A. vol. 1175, pp. 81-88, 2007.
[31]	K. N. Smirnov, I. A. Dyatchkov, and M. V. Telnov, “Effect of monomer mixture composition on structure and chromatographic properties of poly (divinylbenzene-co-ethylvinylbenzene-co-2-hydroxyethyl methacrylate) monolithic rod columns for separation of small molecules,” J. Chromatogr. A. vol. 1218, pp. 5010-5019, 2011.
[32]	J. C. Giddings, Dynamics of Chromatography. Part 1. Principles and Theory, Marcel Dekker: NewYork, 1965.

Cite This Article

Plain Text BibTeX RIS

APA Style

Doudou Zhang, Beijiao Cui, Xiaoya Jiang, Ligai Bai, Haiyan Liu. (2016). Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography. American Journal of Polymer Science and Technology, 2(2), 20-27. https://doi.org/10.11648/j.ajpst.20160202.11

Copy | Download

ACS Style

Doudou Zhang; Beijiao Cui; Xiaoya Jiang; Ligai Bai; Haiyan Liu. Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography. Am. J. Polym. Sci. Technol. 2016, 2(2), 20-27. doi: 10.11648/j.ajpst.20160202.11

Copy | Download

AMA Style

Doudou Zhang, Beijiao Cui, Xiaoya Jiang, Ligai Bai, Haiyan Liu. Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography. Am J Polym Sci Technol. 2016;2(2):20-27. doi: 10.11648/j.ajpst.20160202.11

Copy | Download

@article{10.11648/j.ajpst.20160202.11,
  author = {Doudou Zhang and Beijiao Cui and Xiaoya Jiang and Ligai Bai and Haiyan Liu},
  title = {Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography},
  journal = {American Journal of Polymer Science and Technology},
  volume = {2},
  number = {2},
  pages = {20-27},
  doi = {10.11648/j.ajpst.20160202.11},
  url = {https://doi.org/10.11648/j.ajpst.20160202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpst.20160202.11},
  abstract = {In the present work, polymer monolithic columns were prepared via atom transfer radical polymerization technique by using triallyl isocyanurate as monomer, ethylene glycol dimethacrylate as cross linking agent, polyethyleneglycol 200 and 1,4-butylene glycol as binary porogens, N,N-dimethylformamide as solvent, FeCl2 as catalyst, and CCl4 as initiator. Different monolithic columns were obtained by changing the conditions or columns sizes. These columns were used as the stationary phases of high performance liquid chromatography to investigate the factor effecting on the column efficiency. The effects of column diameter (D), pore size (d), porosity (Ö), and linear velocity of the mobile phase (u) on the performance of polymer monolithic columns were studied and the results certified that the plug-like flow in the column was one of the key factors shaping high performance. Furthermore, a structural hydrodynamic model was introduced to investigate the effects of polymer morphology on the performance of liquid chromatography with monolithic columns.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography
AU  - Doudou Zhang
AU  - Beijiao Cui
AU  - Xiaoya Jiang
AU  - Ligai Bai
AU  - Haiyan Liu
Y1  - 2016/10/14
PY  - 2016
N1  - https://doi.org/10.11648/j.ajpst.20160202.11
DO  - 10.11648/j.ajpst.20160202.11
T2  - American Journal of Polymer Science and Technology
JF  - American Journal of Polymer Science and Technology
JO  - American Journal of Polymer Science and Technology
SP  - 20
EP  - 27
PB  - Science Publishing Group
SN  - 2575-5986
UR  - https://doi.org/10.11648/j.ajpst.20160202.11
AB  - In the present work, polymer monolithic columns were prepared via atom transfer radical polymerization technique by using triallyl isocyanurate as monomer, ethylene glycol dimethacrylate as cross linking agent, polyethyleneglycol 200 and 1,4-butylene glycol as binary porogens, N,N-dimethylformamide as solvent, FeCl2 as catalyst, and CCl4 as initiator. Different monolithic columns were obtained by changing the conditions or columns sizes. These columns were used as the stationary phases of high performance liquid chromatography to investigate the factor effecting on the column efficiency. The effects of column diameter (D), pore size (d), porosity (Ö), and linear velocity of the mobile phase (u) on the performance of polymer monolithic columns were studied and the results certified that the plug-like flow in the column was one of the key factors shaping high performance. Furthermore, a structural hydrodynamic model was introduced to investigate the effects of polymer morphology on the performance of liquid chromatography with monolithic columns.
VL  - 2
IS  - 2
ER  -

Copy | Download

Author Information

Doudou Zhang

College of Pharmaceutical Sciences, Hebei University, Baoding, China
Beijiao Cui

College of Pharmaceutical Sciences, Hebei University, Baoding, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China
Xiaoya Jiang

College of Pharmaceutical Sciences, Hebei University, Baoding, China
Ligai Bai

College of Pharmaceutical Sciences, Hebei University, Baoding, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China
Haiyan Liu

College of Pharmaceutical Sciences, Hebei University, Baoding, China; Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education, Hebei University, Baoding, China

Download PDF

Sections

Plain Text BibTeX RIS

APA Style

Doudou Zhang, Beijiao Cui, Xiaoya Jiang, Ligai Bai, Haiyan Liu. (2016). Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography. American Journal of Polymer Science and Technology, 2(2), 20-27. https://doi.org/10.11648/j.ajpst.20160202.11

Copy | Download

ACS Style

Doudou Zhang; Beijiao Cui; Xiaoya Jiang; Ligai Bai; Haiyan Liu. Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography. Am. J. Polym. Sci. Technol. 2016, 2(2), 20-27. doi: 10.11648/j.ajpst.20160202.11

Copy | Download

AMA Style

Doudou Zhang, Beijiao Cui, Xiaoya Jiang, Ligai Bai, Haiyan Liu. Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography. Am J Polym Sci Technol. 2016;2(2):20-27. doi: 10.11648/j.ajpst.20160202.11

Copy | Download

@article{10.11648/j.ajpst.20160202.11,
  author = {Doudou Zhang and Beijiao Cui and Xiaoya Jiang and Ligai Bai and Haiyan Liu},
  title = {Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography},
  journal = {American Journal of Polymer Science and Technology},
  volume = {2},
  number = {2},
  pages = {20-27},
  doi = {10.11648/j.ajpst.20160202.11},
  url = {https://doi.org/10.11648/j.ajpst.20160202.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpst.20160202.11},
  abstract = {In the present work, polymer monolithic columns were prepared via atom transfer radical polymerization technique by using triallyl isocyanurate as monomer, ethylene glycol dimethacrylate as cross linking agent, polyethyleneglycol 200 and 1,4-butylene glycol as binary porogens, N,N-dimethylformamide as solvent, FeCl2 as catalyst, and CCl4 as initiator. Different monolithic columns were obtained by changing the conditions or columns sizes. These columns were used as the stationary phases of high performance liquid chromatography to investigate the factor effecting on the column efficiency. The effects of column diameter (D), pore size (d), porosity (Ö), and linear velocity of the mobile phase (u) on the performance of polymer monolithic columns were studied and the results certified that the plug-like flow in the column was one of the key factors shaping high performance. Furthermore, a structural hydrodynamic model was introduced to investigate the effects of polymer morphology on the performance of liquid chromatography with monolithic columns.},
 year = {2016}
}

Copy | Download

TY  - JOUR
T1  - Investigation of the Factors Effecting on Column Efficiency of Polymer Monolithic Column Using High Performance Liquid Chromatography
AU  - Doudou Zhang
AU  - Beijiao Cui
AU  - Xiaoya Jiang
AU  - Ligai Bai
AU  - Haiyan Liu
Y1  - 2016/10/14
PY  - 2016
N1  - https://doi.org/10.11648/j.ajpst.20160202.11
DO  - 10.11648/j.ajpst.20160202.11
T2  - American Journal of Polymer Science and Technology
JF  - American Journal of Polymer Science and Technology
JO  - American Journal of Polymer Science and Technology
SP  - 20
EP  - 27
PB  - Science Publishing Group
SN  - 2575-5986
UR  - https://doi.org/10.11648/j.ajpst.20160202.11
AB  - In the present work, polymer monolithic columns were prepared via atom transfer radical polymerization technique by using triallyl isocyanurate as monomer, ethylene glycol dimethacrylate as cross linking agent, polyethyleneglycol 200 and 1,4-butylene glycol as binary porogens, N,N-dimethylformamide as solvent, FeCl2 as catalyst, and CCl4 as initiator. Different monolithic columns were obtained by changing the conditions or columns sizes. These columns were used as the stationary phases of high performance liquid chromatography to investigate the factor effecting on the column efficiency. The effects of column diameter (D), pore size (d), porosity (Ö), and linear velocity of the mobile phase (u) on the performance of polymer monolithic columns were studied and the results certified that the plug-like flow in the column was one of the key factors shaping high performance. Furthermore, a structural hydrodynamic model was introduced to investigate the effects of polymer morphology on the performance of liquid chromatography with monolithic columns.
VL  - 2
IS  - 2
ER  -

Copy | Download