| Peer-Reviewed

Antimicrobial Evaluation of Silver Nanoparticle-Polymer Composites Prepared by Gamma Radiation

Received: 17 October 2016    Accepted: 22 December 2016    Published: 12 January 2017
Views:       Downloads:
Abstract

The aim of the present study was to compare the antimicrobial activity of silver nanoparticle-polymer composites prepared by in situ synthesis of the silver nanoparticles within the polyvinyl pyrrolidone (PVP) hydrogel and by direct addition of the silver nanoparticles into the polymer matrix prepared using gamma radiation technology. The antimicrobial activity of the PVP-nanosilver hydrogels prepared with different concentrations of 30, 50, 70 and 100 ppm silver was tested against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Candida albicans. Hydrogels with 100 ppm nanosilver prepared by in situ reduction of silver resulted in about 3 to 5 log reduction in microbial counts after 3 hours as compared to about 2-log reduction with hydrogels prepared by addition of nanosilver. Comparison of the microbial reduction rates in the presence of two types of hydrogels have shown higher antimicrobial effects of nanosilver prepared by in situ reduction of silver by gamma radiation in the polymer matrix.

Published in American Journal of Polymer Science and Technology (Volume 2, Issue 2)
DOI 10.11648/j.ajpst.20160202.14
Page(s) 40-46
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Silver Nanoparticles, Polyvinyl Pyrrolidone, Gamma Radiation, Antimicrobial Activity

References
[1] Lu Y, Spyra P, Mei Y, Ballauff M, Pich A (2007) Composite Hydrogels: Robust Carriers for Catalytic Nanoparticles. Macromol Chem Phys 208 (3): 254-261.
[2] Murali Mohan Y, Lee K, Premkumar T, Geckeler KE (2007) Hydrogel networks as nanoreactors: A novel approach to silver nanoparticles for antibacterial applications. Polymer 48: 158-164.
[3] Palza H (2015) Antimicrobial polymers with metal nanoparticles. Int J Mol Sci 16: 2099-2116.
[4] Yu H, Xu X, Chen X, Lu T, Zhang P, Jing X (2007) Preparation and antibacterial effects of PVA-PVP hydrogels containing silver nanoparticles. J Appl Polym Sci 103 (1): 125-133.
[5] Kong H, Jang J (2008) Antibacterial properties of novel poly(methyl methacrylate) nanofiber containing silver nanoparticles. Langmuir 24: 2051-2156.
[6] Boonkaew B, Barber PM, Rengpipat S, Supaphol P, Kempf M, He J, John VT and Cuttle L (2014) Development and characterization of a novel, antimicrobial, sterile hydrogel dressing for burn wounds: Single-step production with gamma irradiation creates silver nanoparticles and radical polymerization. J Pharm Sci 103 (10): 3244-3253.
[7] Guzman MG, Dille J, Godet S (2012) Synthesis and antibacterial activity of silver nanoparticles against Gram-positive and Gram-negative bacteria. Nanomedicine 8: 37-45.
[8] Radzig MA, Nadtochenko VA, Koksharova OA, Kiwi J, Lipasova VA, Khmel IA (2013) Antibacterial effects of silver nanoparticles on gram-negative bacteria: Influence on the growth and biofilms formation, mechanisms of action. Colloid Surface B 102: 300-306.
[9] Percival SL, Thomas J, Linton S, Okel T, Corum L, Slone W (2012) The antimicrobial efficacy of silver on antibiotic-resistant bacteria isolated from burn wounds. Int Wound J 9 (5): 488-493.
[10] Rai MK, Desmukh SD, Ingle AP, Gade AK (2012) Silver nanoparticles: The powerful nanoweapon against multi-resistant bacteria. J Applied Microbiol 112: 841-852.
[11] Lamponi S, Leone G, Consumi M, Greco G, Magnani A (2012) In vitro biocompatibility of new PVA-based hydrogels as vitreous body substitutes. J Biomater Sci Polym Ed 23: 555-557.
[12] Soler DM, Rodríguez Y, Correa H, Moreno A, Carrizales L (2012) Pilot scale-up and shelf stability of hydrogel wound dressings obtained by gamma radiation. Radiat Phys Chem 81 (8): 1249–1253.
[13] Rosiak JM, Janik I, Kadlubowski S, Kozicki M, Kujawa P, Stasica P, Ulanski P (2002) Radiation formation of hydrogels for biomedical applications. IAEA-Tech Doc 1324: 5-47.
[14] Ajji Z, Othman I, Rosiak JM (2005) Production of hydrogel wound dressings using gamma radiation. Nucl Instrum Methods Phys Res B 229: 75-380.
[15] Rosiak JM, Ulanski P, Pajewski LA, Yoshii F, Makuuchi K (1995) Radiation formation of hydrogels for biomedical purposes. Some remarks and comments. Radiat Phys Chem 46: 161-168.
[16] Kaplan H, Guner A (2000) Characterization and determination of swelling and diffusion characteristics of poly(n-vinyl-2-pyrrolidone) hydrogels in water. J Appl Polym Sci 78:994-1000.
[17] Singh R, Singh D (2012) Radiation synthesis of PVP/Alginate hydrogel containing nanosilver as wound dressing. J Mater Sci Mater Med 23: 2649-2658.
[18] Benamer S, Mahlous M, Boukrif A, Mansouri B, Larbi Youcef S (2006) Synthesis and characterisation of hydrogels based on poly(vinyl pyrrolidone). Nucl Instrum Methods Phys Res B 248: 284-290.
[19] Wang D, Hill DJT, Rasoul F, Whittaker AK (2011) A study of the swelling and model protein release behaviours of radiation-formed poly(n-vinyl-2-pyrrolidone-co-acrylicacid) hydrogels. Radiat Phys Chem 80: 207-212.
[20] Yu T, Ober CK (2003) Methods for the topographical patterning and patterned surface modification of hydrogels based on hydroxyethyl methacrylate. Biomacromolecules 4: 1126-1131.
[21] Amadeu TP, Seabra AB, deOliveira MG, Costa AM (2007) S-nitrosoglutathione containing hydrogel accelerates rat cutaneous wound repair. J Eur Acad Dermatol Venereol 21: 629-637.
[22] Gwon HJ, Lim YM, Nho YC, Baik SH (2010) Humectants effect on aqueous fluids absorption of γ-irradiated PVA hydrogel followed by freeze thawing. Radiat Phys Chem 79: 650-653.
[23] Singh D, Singh A, Singh R (2015) Polyvinyl pyrrolidone/carrageenan blend hydrogels with nanosilver prepared by gamma radiation for use as an antimicrobial wound dressing. J Biomater Sci Polym Ed 26: 1269-1285.
[24] Krkljes AN, Marinovic-Cincovic MT, Kacarevic-Popovic ZM, Nedeljkovic JM (2007) Radiolytic synthesis and characterization of Ag-PVA nanocomposites. Eur Polym J 43: 2171-2176.
[25] Marambio-Jones C, Hoek EMV (2010) A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J Nanopart Res 12: 1531-1551.
[26] Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramirez JT, Yacaman M (2005) The bactericidal effect of silver nanoparticles. Nanotechnology 16: 2346-2353.
[27] Shrivastava S, Bera T, Roy A, Singh G, Ramachandrarao P, Dash D (2007) Characterization of enhanced antibacterial effects of novel silver nanoparticles. Nanotechnology 18: 225103-225112.
[28] Kvitek L, Panacek A, Soukupova J, Kolar M, Vecerova R, Prucek R, Holecova M, Zboril R (2008) Effect of surfactants and polymers on stability and antibacterial activity of silver nanoparticles (NPs). J Phys Chem C 112: 5825-5834.
Cite This Article
  • APA Style

    Rita Singh, Durgeshwer Singh, Antaryami Singh. (2017). Antimicrobial Evaluation of Silver Nanoparticle-Polymer Composites Prepared by Gamma Radiation. American Journal of Polymer Science and Technology, 2(2), 40-46. https://doi.org/10.11648/j.ajpst.20160202.14

    Copy | Download

    ACS Style

    Rita Singh; Durgeshwer Singh; Antaryami Singh. Antimicrobial Evaluation of Silver Nanoparticle-Polymer Composites Prepared by Gamma Radiation. Am. J. Polym. Sci. Technol. 2017, 2(2), 40-46. doi: 10.11648/j.ajpst.20160202.14

    Copy | Download

    AMA Style

    Rita Singh, Durgeshwer Singh, Antaryami Singh. Antimicrobial Evaluation of Silver Nanoparticle-Polymer Composites Prepared by Gamma Radiation. Am J Polym Sci Technol. 2017;2(2):40-46. doi: 10.11648/j.ajpst.20160202.14

    Copy | Download

  • @article{10.11648/j.ajpst.20160202.14,
      author = {Rita Singh and Durgeshwer Singh and Antaryami Singh},
      title = {Antimicrobial Evaluation of Silver Nanoparticle-Polymer Composites Prepared by Gamma Radiation},
      journal = {American Journal of Polymer Science and Technology},
      volume = {2},
      number = {2},
      pages = {40-46},
      doi = {10.11648/j.ajpst.20160202.14},
      url = {https://doi.org/10.11648/j.ajpst.20160202.14},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajpst.20160202.14},
      abstract = {The aim of the present study was to compare the antimicrobial activity of silver nanoparticle-polymer composites prepared by in situ synthesis of the silver nanoparticles within the polyvinyl pyrrolidone (PVP) hydrogel and by direct addition of the silver nanoparticles into the polymer matrix prepared using gamma radiation technology. The antimicrobial activity of the PVP-nanosilver hydrogels prepared with different concentrations of 30, 50, 70 and 100 ppm silver was tested against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Candida albicans. Hydrogels with 100 ppm nanosilver prepared by in situ reduction of silver resulted in about 3 to 5 log reduction in microbial counts after 3 hours as compared to about 2-log reduction with hydrogels prepared by addition of nanosilver. Comparison of the microbial reduction rates in the presence of two types of hydrogels have shown higher antimicrobial effects of nanosilver prepared by in situ reduction of silver by gamma radiation in the polymer matrix.},
     year = {2017}
    }
    

    Copy | Download

  • TY  - JOUR
    T1  - Antimicrobial Evaluation of Silver Nanoparticle-Polymer Composites Prepared by Gamma Radiation
    AU  - Rita Singh
    AU  - Durgeshwer Singh
    AU  - Antaryami Singh
    Y1  - 2017/01/12
    PY  - 2017
    N1  - https://doi.org/10.11648/j.ajpst.20160202.14
    DO  - 10.11648/j.ajpst.20160202.14
    T2  - American Journal of Polymer Science and Technology
    JF  - American Journal of Polymer Science and Technology
    JO  - American Journal of Polymer Science and Technology
    SP  - 40
    EP  - 46
    PB  - Science Publishing Group
    SN  - 2575-5986
    UR  - https://doi.org/10.11648/j.ajpst.20160202.14
    AB  - The aim of the present study was to compare the antimicrobial activity of silver nanoparticle-polymer composites prepared by in situ synthesis of the silver nanoparticles within the polyvinyl pyrrolidone (PVP) hydrogel and by direct addition of the silver nanoparticles into the polymer matrix prepared using gamma radiation technology. The antimicrobial activity of the PVP-nanosilver hydrogels prepared with different concentrations of 30, 50, 70 and 100 ppm silver was tested against Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Candida albicans. Hydrogels with 100 ppm nanosilver prepared by in situ reduction of silver resulted in about 3 to 5 log reduction in microbial counts after 3 hours as compared to about 2-log reduction with hydrogels prepared by addition of nanosilver. Comparison of the microbial reduction rates in the presence of two types of hydrogels have shown higher antimicrobial effects of nanosilver prepared by in situ reduction of silver by gamma radiation in the polymer matrix.
    VL  - 2
    IS  - 2
    ER  - 

    Copy | Download

Author Information
  • Radiation Dosimetry and Processing Group, Defence Laboratory, Defence Research and Development Organization, Jodhpur, India

  • Radiation Dosimetry and Processing Group, Defence Laboratory, Defence Research and Development Organization, Jodhpur, India

  • Radiation Dosimetry and Processing Group, Defence Laboratory, Defence Research and Development Organization, Jodhpur, India

  • Sections