Oxidative Stress Attenuation in Hiv/Aids Patients on Antiretroviral Drugs by Calyx Juice of Hibiscus sabdariffa Linn (Malvaceae)
Journal of Diseases and Medicinal Plants
Volume 1, Issue 1, April 2015, Pages: 1-7
Received: Apr. 19, 2015; Accepted: Apr. 24, 2015; Published: Apr. 30, 2015
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Franklin Nyenty Tabe, Department of Biomedical Science, Faculty of Science, University of Ngaoundere, Adamawa Region, Cameroon
Nicolas Njintang Yanou, Department of Biomedical Science, Faculty of Science, University of Ngaoundere, Adamawa Region, Cameroon; National School of Agro-Industrial Sciences (Ensai), University of Ngaoundere, Adamawa Region, Cameroon
Armel Herve Nwabo Kamdje, Department of Biomedical Science, Faculty of Science, University of Ngaoundere, Adamawa Region, Cameroon
Aurélie –Solange Agume Ntso, University Institut of Technology, Adamawa Region, Cameroon; The Ngaoundere Regional Hospital, Adamawa Region Cameroon
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The present study was aimed at evaluating the in vivo antioxidant potential of Hibiscus sabdariffa Linnaeus calyx juice (foléré) in HIV/AIDS patients on antiretroviral therapy. In the study, 5g of dried calyces were decocted with 250 mL of fresh tap water at 95°C for 15 minutes. The experimental design was a case-control randomized blinded trial in which, 58 patients on antiretroviral drugs were divided into 2 groups age-and-sex-matched. The experimental group was served a glass of 250 mL calyx juice per day alongside their antiretroviral treatment while the control group was exclusively on antiretroviral drugs with no available placebo. The trial was run for three (3) months after which blood samples were analyzed for the biochemical aspartate aminotransferase (ASAT) and alanine aminotransferase ALAT) and the hematological CD4, white blood cells (WBCs), red blood cells (RBCs), and hemoglobin (HGB)] parameters. The results show an increase in RBCs and WBCs in the experimental group which on the other hand experienced a decrease in CD4 and HGB. The control group experienced a decrease in RBCs, less decrease in HGB, and a higher decrease in CD4, but with increased WBCs. There was a parallel increase in ALAT and ASAT in both groups, more elevated in the control group.
Oxidative stress, HIV/AIDS, Antiretroviral, Antioxidant, Hibiscus sabdariffa Lin
To cite this article
Franklin Nyenty Tabe, Nicolas Njintang Yanou, Armel Herve Nwabo Kamdje, Aurélie –Solange Agume Ntso, Oxidative Stress Attenuation in Hiv/Aids Patients on Antiretroviral Drugs by Calyx Juice of Hibiscus sabdariffa Linn (Malvaceae), Journal of Diseases and Medicinal Plants. Vol. 1, No. 1, 2015, pp. 1-7. doi: 10.11648/j.jdmp.20150101.11
United Nations AIDS (UNAIDS).Workshop on HIV/AIDS and Adult Mortality in Developing Countries. New York, 8-13 September 2003. Available from: http//www.UN/POP/MORT/2003/2.[Accessed 23rd August 2013].
Lizette G.D.V., Rosario G.H., Jorge P.A. 2013. Oxidative stress associated to disease progression and toxicity during antiretroviral therapy in Human Immunodeficiency Virus infection. Journal of Virology and Microbiology [online] 2013:15. Available from: http://www.ibimapublishing.com/journals/JVM/jvm.html. DOI: 10.5171/2013.279685.[Accessed14th February 2014].
Adeniram S.A., Dolapo P.O., Ahmed K.J., Oluwole A.B., Ademola A., Temitope A.N.A. 2012. Serum selenium in HIV patients on different antiretroviral regimen. Greener Journal of Medical Sciences [online] 2(6):163-167.Available from: http://www.gjournals.org. [Accessed 14th February 2014]
Antonella M., Eugenio L.I., Maria G.C., Cinzia B., Antonello M., Daniella C., et al. 2009. Oxidative imbalance in HIV-1 infected patients treated with antiretroviral therapy. Journal of Biomedicine and Biotechnology 2009:7 DOI:10.1155/2009/749575.
Esra B., Umit M.S., Cansin S., Serpil E. and Omer K. 2012. Oxidative stress and antioxidant defense.WAO Journal 5:9-19.
Mady C., Manuel D., Mama S., Augustin N., Max R., Oumar S. 2009. Le bissap (Hibiscus sabdariffa L): composition et principales utilisations. Fruits [online] 64(3): 179-193. Available from: http://www.fruits-journal.org or http://dx.doi.org/10.1051/fruits/2009013. [Accessed 7th October 2013]
Claudia S.G., Fidel T.V.B., Anna E.O.R., José A.G.B. 2012. Antioxidant properties and color of Hibiscus sabdariffa extracts. Ciencia e Investigación Agraria [online] 39(1):79-90. Available from: www.rcia.uc.cl. [Accessed 7th October 2013]
Mahadevan N., Shivali and Pradeep K. 2009. Hibiscus sabdariffa Linn- An overview. Natural Product Radiance 8(1):77-83.
Bahaeldeen B.M., Abdelatif A.S., and Abdel hafiz A.D. 2012. Roselle (Hibiscus sabdariffa L.) in Sudan, cultivation and their uses. Bulletin of Environment, Pharmacology and Life Sciences [online] 1(6):48-54. Available from: www.bepls.com. [Accessed 16th February 2014]
Ijeomah A.U., Ugwuona F.U. and Abdullahi H. 2012. Phytochemical composition and antioxidant properties of Hibiscus sabdariffa and Moringa oleifera. Nigerian Journal of Agriculture, Food and Environment 8(1):10-16.
Alaa G. A-H. 2012. Antioxidant and anti-bacterial activities of Hibiscus sabdariffa L. extracts. African Journal of Food Science [online] 6(21):506-511. Available from: http://www.academicjournals.org/AJFSDOI: 10.5897/AJFS12.099. [Accessed 7th October 2013]
Norhaizan M-E., Fong S.H., Amin I., Chew L.Y. 2010.Antioxidant activity in different parts of the Roselle (Hibiscus sabdariffa L) extracts and potential exploitation of the seeds.Food Chemistry[online] 122(2010): 1055-1060. Available from: http://www.elsevier.com/locate/foodchem. [Accessed 7th October 2013]
Siddhuraju P. and Becker K. 2007. The antioxidant and free radical scavenging activities of processed cowpea (Vignaunguiculata L. Walp) seeds extracts. Food Chemistry 101(1):10-19.
Zhishen J., Mengcheng T and Jianming W. 1999.Determination of flavonoids contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry 64: 555–559
Ozgen M., Reese R.N., Tulio A.Z., Scheerens J.C. and Miller A.R. 2006. Modified 2,2-azino-bis-3-ethylbenzenzothiazoline-6-sulfonic acid (ABTS) method to measure antioxidant capacity of selected small fruits and comparison to ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) methods. Journal of Agricultural and Food Chemistry 54:1151-1157.
Philip M. 2004. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin Journal of Science and Technology 26(2):211-219.
Obirikorang C; Yeboah F; Quaye L. 2010. Antioxidant therapy. A relevant therapeutic adjunct for antiretroviral therapy in PLWHAS in Ghana.Webmed Central. INFECTIOUS DISEASES 1(10): WMC001039.
Pasupathi P., Ramachandran T., Sindhu P.J., Saravanan G., Bakthavathsalam G. 2009. Enhanced oxidative stress markers and antioxidant imbalance in HIV infection and AIDS patients. Journal of Scientific Research [online] 1(2): 370-380. Available from: http://www.banglajol.info/index.php/JSR
Lizette G., Gregorio M., Ivon G., Alicia T., Alejandro A., Giliani A., et al. 2003. Contribution to characterization of oxidative stress in HIV/AIDS patients. Pharmacological Research [online] 47(2003):217-224. Available from: http://www.elsevier.com/locate/yphrs. [Accessed 11th May 2003]
Olufunsho A., Sunday O. O., Joseph A.N., Titilope A.A. 2012. Investigation of the levels of oxidative stress parameters in HIV and HIV-TB co-infected patients. Journal of Infections in Developing Countries 6(1): 79-85.
Lizette G.D.V., Ida G., Alicia T., Alejandro A., Randelys M., Rolando T., et al. 2004. Evaluation of oxidative stress in AIDS pediatric patients.ActaFarmacéuticaBonaerense 23(4):466-471.
Noah T.A., Zachary M.W., and Randy J.N. 2012. Inflammation: mechanisms, costs, and natural variation. Annual Review on Ecology and Systems [online] 43:385-406. Available at: http://www.annualreviews.org. DOI: 10.1146/annurev-ecolsys-040212-092530.
Peter L. 2007. Inflammatory mechanisms: the molecular basis of inflammation and disease. Nutrition Reviews® 65(12):140-146. DOI: 10.1301/nr.2007.dec.S140-S146
Miriam D.N., Sebastian W., Michael A.F., Markus S. H-L., Philip F.S. 2011. Molecular mechanisms of inflammation and tissue injury after major trauma-is complement the “bad guy”? Journal of Biomedical Science [online] 18:90. Available from: http://www.jbiomedsci.com/content/80/90. DOI: 10.1186/1423-0127-18-90.
Soetan K.O. and Oyewole O.E. 2009. The need for adequate processing to reduce the anti nutritional factors in plants used as human foods and animal feeds: A review. African Journal of Food Science [online] 3(9):223-232. Available from: http//www.academicjournals.org/AJFS. [Accessed 8th September 2014]
Negi J.S., Negi P.S., Pant G.J., Rawat M.S., Negi S.K. 2013. Naturally occurring saponins: Chemistry and biology. Journal of Poisonous and Medicinal Plant Research [online] 1(1):001-006.
Harsha K., Preeti P., Manish K.P., Harendra K., Amit C.K., Devi D.J. 2012. Foaming glycosides: A review. IOSR Journal of Pharmacy [online] 2(5):23-28. Available from: http//www.iosrphr.org
Das T.K., Banerjee D., Chakraborty D., Pakira M.C., Shrivastava B. and Kuhad R.C. 2012. Saponin: Role in animal system. Veterinary World [online] 5(4):248-254. Available from: http://www.veterinaryworld.org. [Accessed 27th September 2014]Available from: http//www.apexjournal.org
George F., Zohar K., Harinder P.S.M. and Klaus B. 2002. The biological action of saponins in animal systems: a review. British Journal of Nutrition 88:587-605. DOI: 10.1079/BJN2002725.
Mark J.K. and Prem P. 2004. New insights into erythropoiesis: The roles of folate, vitamin B12 and iron. Annual Review on Nutrition 24:105-31. DOI: 10.1146/annurev.nutr.24.012003.132306.
Vasiliki I.K. 2011. A holistic approach of erythropoiesis and iron in anemia. Health Science Journal ® [online] 5(3):176-181. Available from: http//www.hsj.gr.
Greiner R., Konietzny U., Jany K-D. 2006. Phytate-an undesirable constituent of plant-based foods? Journal für Ernährungsmedizin. [Online] 8(3):18-28. Available from: http//www.aerzteverlagshaus.at. [Accessed 8th September 2014]
Mohd A.H., Marghoob H., Abdelmarouf H.M. 2013. Comparative levels of AST, ALT, ALP and GGT in liver associated diseases. European Journal of Experimental Biology [online] 3(2):280-284. Available from: http//www.pelagiaresearchlibrary.com. [Accessed 27th September 2014]
Philip H. and Johnny C. 2012. What is the real function of the liver function tests? Ulster Medical Journal [online] 81(1):30-36. Available from: http://www.ums.ac.uk. [Accessed 27th September 2014]
Siwang Y., Ka L.C., Wenge L., and Ah-ng K. Plant phenolic compounds: Modulation of cytoprotective enzymes and Nrf2/ARE signaling. In: Cesar G. Fraga (ed.) Plant phenolics and human health. Hoboken, New Jersey: John Wiley and sons Inc.; 2010. P. 402-409.
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