Phytochemical Analysis, Antimicrobial and Antioxidant Study of Nigella sativa L
International Journal of Pharmacy and Chemistry
Volume 2, Issue 2, November 2016, Pages: 39-43
Received: Aug. 30, 2016; Accepted: Nov. 14, 2016; Published: Dec. 17, 2016
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Sania Feroz, Institute of Chemical Sciences, University of Peshawar, K. P. K Peshawar, Pakistan
Ghias Uddin, Institute of Chemical Sciences, University of Peshawar, K. P. K Peshawar, Pakistan
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The current investigation was designed to assess the preliminary phytochemical analysis, antimicrobial and anti-radical effect of crude methanolic extract and isolated fractions of Nigella sativa seeds using established protocol. The phytochemical analysis revealed the presence of alkaloids, terpenoids, tannins, reducing sugars, saponins, steroids, proteins and amino acids, fatty acids and caumarine. The crude methanolic extract and isolated fraction were subjected to antibacterial, antifungal and antioxidant activities. The ethyl acetate and chloroform fractions demonstrated significant (80% and 78%) antioxidant activity. Regarding antibacterial activity of N. sativa, chloroform fraction showed good antibacterial effect as compared to the rest of solvent extracted fractions. Fractions were tested for antifungal activity as well. Amongst all, MeOH fraction showed good antifungal activity against A. N and A. S. n- Hexane showed no activity while other fractions showed moderate activity. The current finding suggests that N. sativa contains potential molecules which exhibited excellent antioxidant and antibacterial effect.
Nigella Sativa, Phytochemical Analysis, Antifungal Activity, Anti Bacterial Activity, Antioxidant Activity
To cite this article
Sania Feroz, Ghias Uddin, Phytochemical Analysis, Antimicrobial and Antioxidant Study of Nigella sativa L, International Journal of Pharmacy and Chemistry. Vol. 2, No. 2, 2016, pp. 39-43. doi: 10.11648/j.ijpc.20160202.16
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Malhotra S and Singh AP. A review of pharmacology of phytochemicals from Indian medicinal plants. Internet J. Altern. Med. (2007) 5: 23-27.
Sharma NK, Ahirwar D, Jhade D and Gupta S. Medicinal and Pharmacological Potential of Nigella sativa. Ethnobot. Leaflets. (2009) 13: 946-955.
Randhawa MA and Alghamdi MS. Anticancer activity of Nigella sativa (Black seed). Am. J. Chin. Med. (2011) 39: 1075-1091.
Philip D, Kaleena PK, Valtivittan K and Kumar CPG. Phytochemical screening and antimicrobial activity of Sansevieria roxburghiana Schult. and Schult. F. Middle-East J. Sci. Res. (2011) 10: 512-518.
Savithramma N, Lingarao M and Suhrulatha D. Screening of medicinal plant for secondary metabolites. Middle-East J. Sci. Res. (2011) 8: 579-584.
Uddin G, Rauf A, Rehman TU and Qaisar M. Phytochemical Screening of Pistacia chinensis var. integerrima. Middle-East J. Sci. Res. (2011) 7 (5): 707-711.
Usman H, Fi A and Usman A. Qualitative Phytochemical Screening and in vitro Antimicrobial Effects of Methanol Stem Bark Extract of Ficus thonningii (Moraceae), Afr. J. Tradit. Complement. Altern. Med. (2009) 6: 289-295.
Uddin G, Ullah W, Rauf A, Siddiqui BS, Rehman TU, Azam S and Qaisar M. Phytochemical screening and antimicrobial activity of Cornus microphylla Wall, ex Roxb, Middle-East J. Sci. Res. (2011) 516-519.
Rauf A, Muhammad N, Khan N, Uddin N, Atif M, and Barkatullah. Antibacterial and Phytotoxic Profile of Selected Pakistani Medicinal Plants. World Appl. Sci. J. (2011) 20: 540-544.
Chandrabhan S, Trivedia SS and Verma SK. Antibacterial efficacy and phytochemical analysis of organic solvent extracts of Calotropis gigantean. J. Chem. Pharm. Res. (2011) 3: 330-336.
Merli M, Devi A, Priya I, Singh RJ, Jeyakumar P. Isolation, identification and antimicrobial activity of bioactive compound from Saraca asoca. Int J Curr Pharm Res 2016; 8 (1): 57-61.
Uddin G, Rauf A, Siddiqui BS and Shah SQ. Preliminary Comparative phytochemical Screening of Diospyros Lotus Stewart, Middle-East J. Sci. Res. (2011) 1: 78-81.
Uddin G and Rauf A. Phytochemical screening and biological activity of the aerial parts of Elaeagnus umbellate. Sci. Res. Essays (2012) 7 (43): 3690-3694.
Martin G, Oswaldo C, José P, Cárdenas CA, Jovanny A, Gómez C. Antifungal activity of chloroform and acetone extracts of Solanum dolichosepalum against Fusarium oxysporum. Int J Pharm Pharm Sci 2016. 8 (8); 373-4.
Rauf A, Khan A, Rasool S, Shah ZA, Saleem M. In-vitro Antifungal activity of three selected Pakistani medicinal plants. Middle-East J Med Plants Res 2012. 1 (2); 41-43.
Velioglu YS, Mazza G, Gao L, Oomah BD. Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. J. Agr. Food Chem. (1998) 46: 4113-4117.
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