The current investigation was designed to assess the preliminary phytochemical analysis, antimicrobial and anti-radical effect of crude methanolic extract and isolated fractions of Nigella sativa seeds using established protocol. The phytochemical analysis revealed the presence of alkaloids, terpenoids, tannins, reducing sugars, saponins, steroids, proteins and amino acids, fatty acids and caumarine. The crude methanolic extract and isolated fraction were subjected to antibacterial, antifungal and antioxidant activities. The ethyl acetate and chloroform fractions demonstrated significant (80% and 78%) antioxidant activity. Regarding antibacterial activity of N. sativa, chloroform fraction showed good antibacterial effect as compared to the rest of solvent extracted fractions. Fractions were tested for antifungal activity as well. Amongst all, MeOH fraction showed good antifungal activity against A. N and A. S. n- Hexane showed no activity while other fractions showed moderate activity. The current finding suggests that N. sativa contains potential molecules which exhibited excellent antioxidant and antibacterial effect.
| Published in | International Journal of Pharmacy and Chemistry (Volume 2, Issue 2) |
| DOI | 10.11648/j.ijpc.20160202.16 |
| Page(s) | 39-43 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Nigella Sativa, Phytochemical Analysis, Antifungal Activity, Anti Bacterial Activity, Antioxidant Activity
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APA Style
Sania Feroz, Ghias Uddin. (2016). Phytochemical Analysis, Antimicrobial and Antioxidant Study of Nigella sativa L. International Journal of Pharmacy and Chemistry, 2(2), 39-43. https://doi.org/10.11648/j.ijpc.20160202.16
ACS Style
Sania Feroz; Ghias Uddin. Phytochemical Analysis, Antimicrobial and Antioxidant Study of Nigella sativa L. Int. J. Pharm. Chem. 2016, 2(2), 39-43. doi: 10.11648/j.ijpc.20160202.16
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Download@article{10.11648/j.ijpc.20160202.16,
author = {Sania Feroz and Ghias Uddin},
title = {Phytochemical Analysis, Antimicrobial and Antioxidant Study of Nigella sativa L},
journal = {International Journal of Pharmacy and Chemistry},
volume = {2},
number = {2},
pages = {39-43},
doi = {10.11648/j.ijpc.20160202.16},
url = {https://doi.org/10.11648/j.ijpc.20160202.16},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijpc.20160202.16},
abstract = {The current investigation was designed to assess the preliminary phytochemical analysis, antimicrobial and anti-radical effect of crude methanolic extract and isolated fractions of Nigella sativa seeds using established protocol. The phytochemical analysis revealed the presence of alkaloids, terpenoids, tannins, reducing sugars, saponins, steroids, proteins and amino acids, fatty acids and caumarine. The crude methanolic extract and isolated fraction were subjected to antibacterial, antifungal and antioxidant activities. The ethyl acetate and chloroform fractions demonstrated significant (80% and 78%) antioxidant activity. Regarding antibacterial activity of N. sativa, chloroform fraction showed good antibacterial effect as compared to the rest of solvent extracted fractions. Fractions were tested for antifungal activity as well. Amongst all, MeOH fraction showed good antifungal activity against A. N and A. S. n- Hexane showed no activity while other fractions showed moderate activity. The current finding suggests that N. sativa contains potential molecules which exhibited excellent antioxidant and antibacterial effect.},
year = {2016}
}
TY - JOUR T1 - Phytochemical Analysis, Antimicrobial and Antioxidant Study of Nigella sativa L AU - Sania Feroz AU - Ghias Uddin Y1 - 2016/12/17 PY - 2016 N1 - https://doi.org/10.11648/j.ijpc.20160202.16 DO - 10.11648/j.ijpc.20160202.16 T2 - International Journal of Pharmacy and Chemistry JF - International Journal of Pharmacy and Chemistry JO - International Journal of Pharmacy and Chemistry SP - 39 EP - 43 PB - Science Publishing Group SN - 2575-5749 UR - https://doi.org/10.11648/j.ijpc.20160202.16 AB - The current investigation was designed to assess the preliminary phytochemical analysis, antimicrobial and anti-radical effect of crude methanolic extract and isolated fractions of Nigella sativa seeds using established protocol. The phytochemical analysis revealed the presence of alkaloids, terpenoids, tannins, reducing sugars, saponins, steroids, proteins and amino acids, fatty acids and caumarine. The crude methanolic extract and isolated fraction were subjected to antibacterial, antifungal and antioxidant activities. The ethyl acetate and chloroform fractions demonstrated significant (80% and 78%) antioxidant activity. Regarding antibacterial activity of N. sativa, chloroform fraction showed good antibacterial effect as compared to the rest of solvent extracted fractions. Fractions were tested for antifungal activity as well. Amongst all, MeOH fraction showed good antifungal activity against A. N and A. S. n- Hexane showed no activity while other fractions showed moderate activity. The current finding suggests that N. sativa contains potential molecules which exhibited excellent antioxidant and antibacterial effect. VL - 2 IS - 2 ER -
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