International Journal of Clinical and Experimental Medical Sciences

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The Possible Protective Effects of Inegy versus Cinnamon Oil on the Aorta of Albino Rats with Experimentally Induced Hyperlipidemia

Received: 31 October 2015    Accepted: 09 December 2015    Published: 15 January 2016
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Abstract

Background: hypercholesterolemia is a predominant risk factor for atherosclerosis. The clinical complications of atherosclerosis could be suppressed when plasma lipids are lowered by hypocholesterolemic agents. The aim of this research is to assess the possible protective effect of the drug Inegy versus cinnamon oil on the histological structure of the abdominal aorta of rats with experimentally induced hyperlipidemia. Materials & Methods: the animals were divided into 2 groups (control and experimental group). Group I (control group) was divided into: subgroup I a; received standard diet only, subgroup Ib received Inegy 10 mg/kg body weight / day orally and subgroup Ic received cinnamon oil orally. Group II (The experimental group) was divided into: subgroup II, which received a high fat high cholesterol (HFHC) diet, subgroup II b received simultaneously both HFHC diet and Inegy and subgroup II c which received simultaneously both HFHC diet and cinnamon oil. After 3 months blood samples were collected from all rats for cholesterol and triglyceride estimation and fresh specimens were taken from the abdominal aorta of each rat and processed for; light microscopic, immunohistochemical and morphometric studies. Results: Significant increase of serum cholesterol and triglyceride levels in hyperlipidemic rats (subgroup II a) was detected. Moreover, evident structural changes in the form of endothelial irregularity, focal loss and shedding of large areas of denuded endothelium were illustrated. It was associated with adhesion of mononuclear leukocytes and increased foam macrophages. Examination of histological sections of the abdominal aorta of (subgroups II b and II c) showed pronounced amelioration of the structural changes depicted in the aorta of the hyperlipidemic subgroup. Conclusion: HFHC diet induced evident biochemical, histological and immunohistochemical changes in the rat aorta. It was also concluded that both Inegy and cinnamon oil offered good protection against the histopathological changes induced by HFHC diet. There were no statistical significant differences between the protective effect of both agents as regards the biochemical and morphometric results.

DOI 10.11648/j.ijcems.20150104.12
Published in International Journal of Clinical and Experimental Medical Sciences (Volume 1, Issue 4, November 2015)
Page(s) 78-91
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Hyperlipidemia, Atherosclerosis, Inegy Drug, Cinnamon Oil, Aorta, Rats

References
[1] Farmer JA, Gotto AM. Dyslipidemia and the vulnerable plaque. Prog Cardiovasc Dis. 2002; 44(6): 415-428.
[2] Kwok CY, Wong CNY, Yau MYC, Fu Yu PH, Shan Au AL, Wa Poon CC, et al. Consumption of dried fruit of Crataegus pinnatifida (hawthorn) suppresses high-cholesterol diet-induced hypercholesterolemia in rats. J Funct Foods. 2010; 2: 179-186.
[3] American Heart Association. American Heart Association Low–Fat, Low–Cholesterol Cookbook, 3rd Edition: Delicious Recipes to Help Lower Your Cholesterol. New York, NY: Clarkson Potter, 2005.
[4] Kosoglou T, Meyer I, Veltri EP, Statkevich P, Yang B, Zhu Y, et al. Pharmacodynamic interaction between the new selective cholesterol absorption inhibitor ezetimibe and simvastatin. Br J Clin Pharmacol. 2002; 54: 309-319.
[5] Shovman O, Levy Y, Gilburd B, Shoenfeld Y. Anti-inflammatory and immunomodulatory properties of statins. Immunol Resp. 2002; 25: 271-285.
[6] Catapano AL, Catapano L, Fellin R. Intestinal cholesterol absorption: a pharmacological target for lowering of plasma cholesterol. Ital Heart J. 2004; 5(10): 779-84.
[7] Huang JC, Lee TY, Lio MJ, et al. Begin with the real –word patients of non – goal – achieved hypercholesterolemia in Taiwan through the ezetimibe / simvastatin tablet – The BRAVO study. Curr Med Res Opin. 2011; 27(8): 1645-1651.
[8] Garjani A, Fathiazad F, Zakheri A, et al. The effect of total extract of Securigera securidaca L. seeds on serum lipid profiles, antioxidant status and vascular function in hypercholesteremic rat. J Ethnopharmaco. 2009; 126(3): 525-532.
[9] Khan A, Safdar M, Ali Khan MM, Khattak KN, Anderson RA. Cinnamon improves glucose and lipids of people with type 2 diabetes. Diabetes Care. 2003; 26(12): 3215-3218.
[10] Lee YH, Kim MJ, Choi CI, Bae JW, Janh CG, Lee SY. Retrospective study on antihyperlipidemic efficacy and safety of simvastatin, ezetimibe and their combination in Korean adults. Arch Pharm Res. 2011; 34(8): 1331-1337.
[11] Rajyalakshmi G, Reddy A, Rajesham VV. A comparative antihyperlipidemic activity of atorvastatin with simvastatin in rats. The internet journal of pharmacology. 2009: 6(2).
[12] Ghorbani A, Shafiee - Nik R, Rakhshandeh H, Borji A. Antihyperlipidemic effect of a polyherbal mixture in streptozotocin – induced diabetic rats. J Lipids. 2013(2013), Article ID 675759, 6 pages. University press; 1980: 140-222.
[13] Bozzola JJ, Russell LD. Principles and Techniques, Specimens preparation for transmission EM. 1st ed. Boston London: John and Bartlett publishers; 1992: 16-47.
[14] Millonig G. Advantages of a phosphate buffer for OSO4 solutions in fixation. JAPPL Physics. 1961; 32: 1637.
[15] Van Noorden S. Principles in immunostaining. In: Fillip M, Lake B. Histochemistry in pathology. 2nd ed. Edinburgh, London, Melbourne, new York: Churchill livingstone; 1990: 31.
[16] Shehata A, Yousef O. Physiological studies on the risk factors responsible for atherosclerosis in rat. Nature and Science. 2010; 8(5): 144-151.
[17] EL-Beshbishy HA, Singab AN, Sinkkonen J, Pihlaja K. Hypolipidemic and antioxidant effects of Morus alba L. (Egyptian mulberry) root bark fractions supplementation in cholesterol fed rats. Life Sci. 2006; 78: 2724-2733.
[18] Dimitrova-Shumkovska J, Veenman L, Ristoski T, Leschiner S, Gavish M. Chronic high fat, high cholesterol supplementation decreases 18 kDa translocator protein binding capacity in association with increased oxidative stress in rat liver and aorta. Food Chem Toxicol. 2010; 48: 910-921.
[19] Amin KA, Abd El-Twab TM. Oxidative markers, nitric oxide and homocysteine alteration in hypercholesterolimic rats: role of atorvastatine and cinnamon. Int J Clin Exp. Med 2009; 2: 254-265.
[20] Castillo SS, Doger MM, Bolkent S, Yanardag R. Cholesterol efflux and the effect of combined treatment with niacin and chromium on aorta of hyperlipidemic rat. Mol Cell Biochem.2008; 308: 151-159.
[21] Cooke JP. Role of nitric oxide in progression and regression of atherosclerosis. West J Med. 1996; 164: 419-424.
[22] Vogiatzi G, Tousoulis D, Stefanadis C. The role of oxidative stress in atherosclerosis. Hellenic J Cardiol. 2009; 50: 402-409.
[23] Martinet W, Knaapen MW, De Meyer GR, Herman AG, Kockx MM. Oxidative DNA damage and repair in experimental atherosclerosis are reversed by dietary lipid lowering. Circ Res. 2001; 88: 733-739.
[24] Deepa PR, Varalakshmi P. Atheroprotective effect of exogenous heparin-derivative treatment on the aortic disturbances and lipo-protein oxidation in hypercholesterolemic diet fed rats. Clin Chim Acta. 2005; 355: 119-130.
[25] Mahfouz MM, Kummerow FA. Cholesterol-rich diets have different effects on lipid peroxide-tion, cholesterol oxides, and antioxidant enzymes in rats and rabbits. J Nutr Biochem. 2000; 11: 293-302.
[26] Kapoor P, Ansari MN, Bhandari U. Modulatory effect of curcumin on methionine-induced hyperlipidemia and hyperhomocysteinemia in albino rats. Indian J Exr Biol. 2008; 46: 534-540.
[27] Ratheesh M, Shyni GL, Sindhu G. Helen A. Inhibitory effect of Ruta graveolens L. on oxidative damage, inflammation and aortic pathology in hypercholesteromic rats. Exp Toxicol Pathol; 2010.
[28] Rudijanto A. The role of vascular smooth muscle cells on the pathogenesis of atherosclerosis. Acta Med Indones. 2007; 39: 86-93.
[29] Sternlicht MD, Werb Z. How matrix metalloproteinases regulate cell behaviour. Annu Rev Cell Dev Biol. 2001; 17: 463-516.
[30] Jones R, Jacobson M, Steudel W. alpha- smooth muscle cells in pulmonary hypertension. Am J Respir Cell Mol Biol. 1999; 20: 582-594.
[31] Ishikawa H, Uga S, Mashimo K, Yoshitomi T, Kusanagi M, Shimizu K. pharmacological vascular reactivity in isolated hypercholesterolemic rabbit ciliary artery. Exp Eye Res. 2004; 4: 805-813.
[32] Aguila MB, Mandarim CA. Aorta wall quantitative alterations due to different long-term high-fat diet in rats. Food Chem Toxicol. 2003; 41: 1391-1397.
[33] Jimi S, Saku K, Uesugi N, Sakata N, Takebayashi S. Oxidized low density lipoprotein stimulates collagen production in cultured arterial smooth muscle cells. Atherosclerosis. 1995; 116: 15–26.
[34] Cerqueira N, Yoshida W, Müller S, Sequeira J, Rodrigues A, Padovani C. Morphological and biomechanical study of abdominal aorta of rats submitted to experimental chronic alcoholism. Acta Cir Bra. 2005; 20(3): 213-218.
[35] Isenburg J, Simionescu D, Starcher B, Vyavahare N. Elastin stabilization for treatment of abdominal aortic aneurysms. Circulation. 2007; 115: 1729-737.
[36] Nigris F, Mancini FP, Balestrieri ML, et al. Therapeutic dose of nebivolol, a nitric oxide releasing β-blocker, reduces atherosclerosis in cholesterol-fed rabbits. Nitric Oxide. 2008; 19: 57-63.
[37] Vardi N, Ozturk F, Fadillioglu E, Otlu A, Yagmurca M. Histological changes in the rat thoracic aorta after chronic nitric oxide synthase inhibition. Turk J Med Sci. 2003; 33: 141-147.
[38] Hazra B, Biswas S, Mandal N. Antioxidant and free radical scavenging activity of Spondias pinnata. BMC Complement Altern Med. 2008; 8: 63.
[39] Shepherd J. Lipids in health and disease. Biochem Soc Trans. 2004; 32: 1051-1056.
[40] Sato A, Watanabe K, Fukuzumi H, Hase K, Ishida F, Kamei T. Effect of simvastatin (MK-733) on plasma triacylglycerol levels in rats. Biochem pharmacol. 1991; 15; 41(8): 1163-1172.
[41] Suanarunsawat T, Ayutthaya W, Songsak T, Thirawarapan S, Poungshompoo S. Antioxidant effect and lipid lowering effect of essential oils extracted from Ocimum sanctum L. leaves in rats fed with high cholesterol diet. J Clin Biochem Nutr. 2010; 46: 52-59.
[42] Antoniades C, Tousoulis D, Stefanadis C. Effect of endothelial nitric oxide synthase gene polymorphisms on oxidative stress, inflammatory status and coronary atherosclerosis: an example of transient phenotype. J Am Coll Cardiol. 2007; 49: 1226.
[43] Jovanovic SV, Simic MG. Antioxidants in nutrition. Ann NY Acad Sci. 2000; 899: 326-234.
[44] Li j, Liu T, Wang L, et al. Antihyperglycemic and hyperlipidemic action of cinnamaldehyde in C57BL / Ks db / db mice. J Tradit Chin Med. 2012; 32(3): 446-452.
[45] Kim SH, Choung SY. Antihyperglycemic and hyperlipidemic action of Cinnamomi Cassiae (Cinnamon bark) extract in C57BL / Ks db / db mice. Aech Pharm Res. 2010; 33(2): 325-333.
[46] Schmidt E, Kitzing K, Schloss HA. Composition and antioxidant activities of the essential oil of cinnamon (cinnamomum zeylanicum blume) leaves from sri lanka. Jeobp. 2006; 9(2): 170-182.
[47] Sheng X, Zhang Y, Gong Z, Huang C, Zang YQ. Improved insulin resistance and lipid metabolism by cinnamon extract through activation of peroxisome proliferator-activated receptors. PPAR Res. 2008; 2008: 581348.
[48] Lee JS, Jeon SM, Park EM, et al. Cinnamate supplementation enhances hepatic lipid metabolism and antioxidant defense system. J Med Food. 2003; 6: 183-191.
[49] Anderson RA, Broadhurst CL, Polansky MM, et al. Isolation and characterization of polyphenol type-A polymers from cinnamon with insulin-like biological activity. J Agric Food Chem. 2004; 52(1): 65-70.
[50] Kannappan S, Jayaraman T, Rajasekar P, Ravichandran MK, Anuradha CV. Cinnamon bark extract improves glucose metabolism and lipid profile in the fructose-fed rat. Singapore Med J. 2006; 47(10): 858-863.
[51] Murcia MA, Egea I, Romojaro F, Parras P, Jimenez AM, Martinez- Tome M. Antioxidant evaluation in dessert spices compared with common food additives. Influence of irradiation procedure. J Agric Food Chem. 2004; 52: 1872-1881.
Author Information
  • Department of Histology and Cell Biology, Faculty of Medicine, Alexandria University, Alexandria, Egypt

  • Department of Histology and Cell Biology, Faculty of Medicine, Alexandria University, Alexandria, Egypt

  • Department of Histology and Cell Biology, Faculty of Medicine, Alexandria University, Alexandria, Egypt

  • Department of Histology and Cell Biology, Faculty of Medicine, Alexandria University, Alexandria, Egypt

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    Moushira M. Safi, Maha W. Abou-Nazel, Fardous S. Karawya, Amira M. Omar. (2016). The Possible Protective Effects of Inegy versus Cinnamon Oil on the Aorta of Albino Rats with Experimentally Induced Hyperlipidemia. International Journal of Clinical and Experimental Medical Sciences, 1(4), 78-91. https://doi.org/10.11648/j.ijcems.20150104.12

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    Moushira M. Safi; Maha W. Abou-Nazel; Fardous S. Karawya; Amira M. Omar. The Possible Protective Effects of Inegy versus Cinnamon Oil on the Aorta of Albino Rats with Experimentally Induced Hyperlipidemia. Int. J. Clin. Exp. Med. Sci. 2016, 1(4), 78-91. doi: 10.11648/j.ijcems.20150104.12

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    AMA Style

    Moushira M. Safi, Maha W. Abou-Nazel, Fardous S. Karawya, Amira M. Omar. The Possible Protective Effects of Inegy versus Cinnamon Oil on the Aorta of Albino Rats with Experimentally Induced Hyperlipidemia. Int J Clin Exp Med Sci. 2016;1(4):78-91. doi: 10.11648/j.ijcems.20150104.12

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  • @article{10.11648/j.ijcems.20150104.12,
      author = {Moushira M. Safi and Maha W. Abou-Nazel and Fardous S. Karawya and Amira M. Omar},
      title = {The Possible Protective Effects of Inegy versus Cinnamon Oil on the Aorta of Albino Rats with Experimentally Induced Hyperlipidemia},
      journal = {International Journal of Clinical and Experimental Medical Sciences},
      volume = {1},
      number = {4},
      pages = {78-91},
      doi = {10.11648/j.ijcems.20150104.12},
      url = {https://doi.org/10.11648/j.ijcems.20150104.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ijcems.20150104.12},
      abstract = {Background: hypercholesterolemia is a predominant risk factor for atherosclerosis. The clinical complications of atherosclerosis could be suppressed when plasma lipids are lowered by hypocholesterolemic agents. The aim of this research is to assess the possible protective effect of the drug Inegy versus cinnamon oil on the histological structure of the abdominal aorta of rats with experimentally induced hyperlipidemia. Materials & Methods: the animals were divided into 2 groups (control and experimental group). Group I (control group) was divided into: subgroup I a; received standard diet only, subgroup Ib received Inegy 10 mg/kg body weight / day orally and subgroup Ic received cinnamon oil orally. Group II (The experimental group) was divided into: subgroup II, which received a high fat high cholesterol (HFHC) diet, subgroup II b received simultaneously both HFHC diet and Inegy and subgroup II c which received simultaneously both HFHC diet and cinnamon oil. After 3 months blood samples were collected from all rats for cholesterol and triglyceride estimation and fresh specimens were taken from the abdominal aorta of each rat and processed for; light microscopic, immunohistochemical and morphometric studies. Results: Significant increase of serum cholesterol and triglyceride levels in hyperlipidemic rats (subgroup II a) was detected. Moreover, evident structural changes in the form of endothelial irregularity, focal loss and shedding of large areas of denuded endothelium were illustrated. It was associated with adhesion of mononuclear leukocytes and increased foam macrophages. Examination of histological sections of the abdominal aorta of (subgroups II b and II c) showed pronounced amelioration of the structural changes depicted in the aorta of the hyperlipidemic subgroup. Conclusion: HFHC diet induced evident biochemical, histological and immunohistochemical changes in the rat aorta. It was also concluded that both Inegy and cinnamon oil offered good protection against the histopathological changes induced by HFHC diet. There were no statistical significant differences between the protective effect of both agents as regards the biochemical and morphometric results.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - The Possible Protective Effects of Inegy versus Cinnamon Oil on the Aorta of Albino Rats with Experimentally Induced Hyperlipidemia
    AU  - Moushira M. Safi
    AU  - Maha W. Abou-Nazel
    AU  - Fardous S. Karawya
    AU  - Amira M. Omar
    Y1  - 2016/01/15
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ijcems.20150104.12
    DO  - 10.11648/j.ijcems.20150104.12
    T2  - International Journal of Clinical and Experimental Medical Sciences
    JF  - International Journal of Clinical and Experimental Medical Sciences
    JO  - International Journal of Clinical and Experimental Medical Sciences
    SP  - 78
    EP  - 91
    PB  - Science Publishing Group
    SN  - 2469-8032
    UR  - https://doi.org/10.11648/j.ijcems.20150104.12
    AB  - Background: hypercholesterolemia is a predominant risk factor for atherosclerosis. The clinical complications of atherosclerosis could be suppressed when plasma lipids are lowered by hypocholesterolemic agents. The aim of this research is to assess the possible protective effect of the drug Inegy versus cinnamon oil on the histological structure of the abdominal aorta of rats with experimentally induced hyperlipidemia. Materials & Methods: the animals were divided into 2 groups (control and experimental group). Group I (control group) was divided into: subgroup I a; received standard diet only, subgroup Ib received Inegy 10 mg/kg body weight / day orally and subgroup Ic received cinnamon oil orally. Group II (The experimental group) was divided into: subgroup II, which received a high fat high cholesterol (HFHC) diet, subgroup II b received simultaneously both HFHC diet and Inegy and subgroup II c which received simultaneously both HFHC diet and cinnamon oil. After 3 months blood samples were collected from all rats for cholesterol and triglyceride estimation and fresh specimens were taken from the abdominal aorta of each rat and processed for; light microscopic, immunohistochemical and morphometric studies. Results: Significant increase of serum cholesterol and triglyceride levels in hyperlipidemic rats (subgroup II a) was detected. Moreover, evident structural changes in the form of endothelial irregularity, focal loss and shedding of large areas of denuded endothelium were illustrated. It was associated with adhesion of mononuclear leukocytes and increased foam macrophages. Examination of histological sections of the abdominal aorta of (subgroups II b and II c) showed pronounced amelioration of the structural changes depicted in the aorta of the hyperlipidemic subgroup. Conclusion: HFHC diet induced evident biochemical, histological and immunohistochemical changes in the rat aorta. It was also concluded that both Inegy and cinnamon oil offered good protection against the histopathological changes induced by HFHC diet. There were no statistical significant differences between the protective effect of both agents as regards the biochemical and morphometric results.
    VL  - 1
    IS  - 4
    ER  - 

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