The aim of this study is the application of Alkaline Phosphatase (ALP) and Gamma-glutamyl Transferase (GGT) activity, by using the corresponding kinetic methods, at the Clinical Chemistry Laboratories in our place. Enzymes are proteins synthesized in the cells of living organisms in order to play the role of bio-catalysts. Today are recognized about 2000 different enzymes. Some of those have been isolated in pure form of the chemical. We used serum as a material and we took into consideration 15 healthy patients, 15 patients who had viral hepatitis and 15 patients with idiopathic hyper-bilirubinemia. Solution where the substance is being analyzed, was treated with specific reagents according to the procedures such as incubation, heating, etc, until the color is formed. The absorbance of the coloring solution was measured using photometry at a wavelength of 405nm. As an analytical method for the determination of ALP, we chose 4-nitrophenylphosphate (4NPP) as a substrate, while as a buffer for the optimal pH adjustment of the reaction we used 2-amino-2 methyl -1-propanol (AMP). ALP was within permitted values from 100 to 290 U /l, whereas Gamma-glutamyl Transferase was at normal reference values of 5 to 51 U /l. Automated method was equal to the standard method, with a correlation coefficient of r = 0997. Accuracy within the series of measurements had a variation coefficient of only 1.25%. From the results taken from the laboratories, we had an increased precision, accuracy, sensitivity and reliability as a result of the technological process of electronic engineering of new modern techniques with monoclonal antibodies and immobilized enzymes.
| Published in | International Journal of Science and Qualitative Analysis (Volume 1, Issue 1) |
| DOI | 10.11648/j.ijsqa.20150101.12 |
| Page(s) | 6-10 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Clinical Chemistry, ALP Determination, GGT Assay Kit, Enzyme Classification, Hepatic Enzymes, Photometry
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APA Style
Alma Daja, Entela Treska. (2015). The Impact of Enzymes in the Hepatic Function. International Journal of Science and Qualitative Analysis, 1(1), 6-10. https://doi.org/10.11648/j.ijsqa.20150101.12
ACS Style
Alma Daja; Entela Treska. The Impact of Enzymes in the Hepatic Function. Int. J. Sci. Qual. Anal. 2015, 1(1), 6-10. doi: 10.11648/j.ijsqa.20150101.12
AMA Style
Alma Daja, Entela Treska. The Impact of Enzymes in the Hepatic Function. Int J Sci Qual Anal. 2015;1(1):6-10. doi: 10.11648/j.ijsqa.20150101.12
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Download@article{10.11648/j.ijsqa.20150101.12,
author = {Alma Daja and Entela Treska},
title = {The Impact of Enzymes in the Hepatic Function},
journal = {International Journal of Science and Qualitative Analysis},
volume = {1},
number = {1},
pages = {6-10},
doi = {10.11648/j.ijsqa.20150101.12},
url = {https://doi.org/10.11648/j.ijsqa.20150101.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijsqa.20150101.12},
abstract = {The aim of this study is the application of Alkaline Phosphatase (ALP) and Gamma-glutamyl Transferase (GGT) activity, by using the corresponding kinetic methods, at the Clinical Chemistry Laboratories in our place. Enzymes are proteins synthesized in the cells of living organisms in order to play the role of bio-catalysts. Today are recognized about 2000 different enzymes. Some of those have been isolated in pure form of the chemical. We used serum as a material and we took into consideration 15 healthy patients, 15 patients who had viral hepatitis and 15 patients with idiopathic hyper-bilirubinemia. Solution where the substance is being analyzed, was treated with specific reagents according to the procedures such as incubation, heating, etc, until the color is formed. The absorbance of the coloring solution was measured using photometry at a wavelength of 405nm. As an analytical method for the determination of ALP, we chose 4-nitrophenylphosphate (4NPP) as a substrate, while as a buffer for the optimal pH adjustment of the reaction we used 2-amino-2 methyl -1-propanol (AMP). ALP was within permitted values from 100 to 290 U /l, whereas Gamma-glutamyl Transferase was at normal reference values of 5 to 51 U /l. Automated method was equal to the standard method, with a correlation coefficient of r = 0997. Accuracy within the series of measurements had a variation coefficient of only 1.25%. From the results taken from the laboratories, we had an increased precision, accuracy, sensitivity and reliability as a result of the technological process of electronic engineering of new modern techniques with monoclonal antibodies and immobilized enzymes.},
year = {2015}
}
TY - JOUR T1 - The Impact of Enzymes in the Hepatic Function AU - Alma Daja AU - Entela Treska Y1 - 2015/05/28 PY - 2015 N1 - https://doi.org/10.11648/j.ijsqa.20150101.12 DO - 10.11648/j.ijsqa.20150101.12 T2 - International Journal of Science and Qualitative Analysis JF - International Journal of Science and Qualitative Analysis JO - International Journal of Science and Qualitative Analysis SP - 6 EP - 10 PB - Science Publishing Group SN - 2469-8164 UR - https://doi.org/10.11648/j.ijsqa.20150101.12 AB - The aim of this study is the application of Alkaline Phosphatase (ALP) and Gamma-glutamyl Transferase (GGT) activity, by using the corresponding kinetic methods, at the Clinical Chemistry Laboratories in our place. Enzymes are proteins synthesized in the cells of living organisms in order to play the role of bio-catalysts. Today are recognized about 2000 different enzymes. Some of those have been isolated in pure form of the chemical. We used serum as a material and we took into consideration 15 healthy patients, 15 patients who had viral hepatitis and 15 patients with idiopathic hyper-bilirubinemia. Solution where the substance is being analyzed, was treated with specific reagents according to the procedures such as incubation, heating, etc, until the color is formed. The absorbance of the coloring solution was measured using photometry at a wavelength of 405nm. As an analytical method for the determination of ALP, we chose 4-nitrophenylphosphate (4NPP) as a substrate, while as a buffer for the optimal pH adjustment of the reaction we used 2-amino-2 methyl -1-propanol (AMP). ALP was within permitted values from 100 to 290 U /l, whereas Gamma-glutamyl Transferase was at normal reference values of 5 to 51 U /l. Automated method was equal to the standard method, with a correlation coefficient of r = 0997. Accuracy within the series of measurements had a variation coefficient of only 1.25%. From the results taken from the laboratories, we had an increased precision, accuracy, sensitivity and reliability as a result of the technological process of electronic engineering of new modern techniques with monoclonal antibodies and immobilized enzymes. VL - 1 IS - 1 ER -
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