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Bioethanol Production from Rice Winery Cake Using Lactic Acid Bacteria and Yeasts by the Process of Simultaneous Saccharification and Fermentation

Received: 28 October 2016    Accepted: 29 November 2016    Published: 27 December 2016
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Abstract

Bioethanol is a sustainable energy source which serves as an alternative to fossil fuel and contributes to a clean environment. Bioethanol was produced by individual activities of lactic acid bacteria and yeasts from rice cake waste using a simultaneous saccharification and fermentation process. The rice cake waste is a filtered solid waste of fermented rice wine mash and contains 78.04% of total carbohydrate, 10.88% of protein, 2.26% of ash, 8.12% of moisture and 0.7% of fat. The rice cake was mixed with raw starch digesting enzyme of Aspergillus niger and (Lactobacillus fermentum, Rhodotorula minuta, Rhodotorula mucilagnosa, Candida krusei, Kodamara ohmeri) respectively into different fermenting chambers. Rhodotorula minuta produced the highest efficiency of ethanol of 52.06% at the temperature of 30°C and pH of 2.58. Reducing sugar was observed to decrease with increase in bioethanol production and cell growth increased as the fermentation time increases. Bioethanol can also be produced from rice cake waste of a fermented rice mash, which can serve as a bio fuel and contributes to a healthy environment.

Published in International Journal of Microbiology and Biotechnology (Volume 1, Issue 1)
DOI 10.11648/j.ijmb.20160101.15
Page(s) 33-39
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Rice Cake, Simultaneous Saccharification Fermentation, Rhodotorula minuta, Aspergillus niger

References
[1] Keun, K., Hanh, V., Vu. (2009). Ethanol Production from Rice Winery Cake by Simultaneous Saccharification and Fermentation without Cooking. Journal of Microbiology and Biotechnology. 19 (10): 1161-1168.
[2] Nwabueze, T. U., and Otunwa, U. (2006). Effect of Supplementation of African Breadfruit (Treculia africana) Hulls with Organic Wastes on Growth Characteristics of Saccharomyces cerevisiae. African Journal of Biotechnology. 5: 1494-1498.
[3] Adesanya, O. A., Oluyemi, K. A., Josiah, S. J., Adesanya, R, A., Shittu, L. A. J., Ofusori, D. A., Bankole, M. A., and Babalola, G. B. (2008). Ethanol Production by Saccharomyces cerevisiae from Cassava Peel Hydrolysate. International Journal of Microbiology 5 (1).
[4] Akin-osanaiye, B. C., Nzelibe, H, C., and Agbaji, A. S. (2005). Production of Ethanol from Carica papaya (pawpaw) Agro Waste: Effect of Saccharification and Different Treatments on Ethanol Yield. African Journal of Biotechnology. 4: 657-659.
[5] Tang, Y. Q., Yoji, K., Kai, Z., Ming, M., Shigeru, M., Xiao, L. W., And Kenji, K. (2008). Ethanol Production from Kitchen Waste using the Flocculating Yeast Saccharomyces cerevisiae Starin Kf-7. Biomas Biomeer 32: 1037-1045.
[6] Rudravaran, R., Lakshmi, M., Venkateswar, R., and Ravindra, P. (2007). Economics and Environmental Impact of Bioethanol Technologies: An appraisal on Biomass and Bio-energy. 2: 14-32.
[7] Shubhra, T., Jadhav, S. K., and Tiwari, K. L. (2013). Comparative Study of Bioethanol Production from Different Carbohydrate Sources. http://www.sciencepub.net/researcher. 2013; 5 (12).
[8] AOAC, (2005). Official Methods for Analysis, 16th Edition. Association of Official Analytical Chemists.
[9] Singh A. K., Rath S., Kumar Y., Masih H., Peter J. K., Benjamin J. C., Kumar P. S., Sigh D, P. (2014). Bio-Ethanol Production from Banana Peel by Simultaneous Saccharification and Fermentation Process using co-cultures Aspergillus niger and Saccharomyces cerevisiae. International Journal of Current Microbiology and Applied Sciences. 3 (5): 84-96.
[10] Khaing, T. W., Weine, N., Mya, M. O. (2008). Isolation, Characterization and Screening of Thermo Tolerant, Ethanol Tolerant Indigenous Yeasts and Study on the Effectiveness of Immobilized Cell for Ethanol Production. Journal of Science and Technology. 1: 12-14.
[11] Karthikeyan, R., Ravichandiran, K., Ramakrishnan, T. (2014). Production of Wine from Tamil Nadu Traditional Rice Varieties. International Food Research Journal 21 (6): 2091-2093.
[12] Marx, S., and Nquma, T. Y. (2013). Cassava as Feedstock for Ethanol Production in South Africa. African Journal of Biotechnology. 12 (31): 4475-4983.
[13] Ado, S, D., Kachalla, G. U., Tijjani, M, B., and Aliyu, M. S. (2009). Ethanol Production from Corn Cobs by Co-Cultures of Saccharomyces cerevisiae and Aspergillus niger. Bayero Journal of Pure and Applied Sciences 12 (2): 99-101.
[14] Itelima, J., Onuwuliri, F., Onuwuliri, E., Issac, O., and Ofarji, S. (2013). Bioethanol production from banana, plantain and Pineapple Peels by Simultaneous Saccharification and Fermentation Process. International Journal of Environmental Science and Development. 4 (2).
[15] Chim, C., Erlinda, I. D., Elegado, F. B., Hurtada, A. W., Chakrya, N., and Raymond, C. L. (2015). Traditional Dried Starter Culture (Medombae) for Rice Liquor Production in Cambodia. International Food Research Journal. 22 (4): 1642-1650.
[16] Chongkhong, S., and Lolharat, B. (2013). Bioethanol from Prebiotic Extracted Jackfruit Seeds. The 6th PSU-UNS International Conference on Engineering and Technology (ICET-2013), Novi Sad, Serbia, may 15-17, 2013, University Of Novi Sad, Faculty of Technical Sciences.
[17] Verma, G., Nigam, P., Singh, D., and Chaudhary, K. (2000). Bioconversion of Starch to Ethanol in a Single-Step Process by Co-Cultures of amylolytic yeasts and Saccharomyces cerevisiae 21. Bio-resource Technology. 72: 261-266.
[18] Hadeel, A., Hossain, A. B. M. S. Latifa, K., ALNaqeb, H., Abear, J., and Al-Hewiti, N. (2011). Bioethanol Fuel Production from Rambutan Fruit Biomass as Reducing Agent of Global Warming and Greenhouse Gases. African Journal of Biotechnology. 10 (50): 10157-10165.
[19] Manikandan, K. and Viruthagiri, T. (2010). Kinetic and Optimization Studies on Ethanol Production from Corn Flour. International Journal of Chemical and Biological Engineering 3 (2): 65-69.
[20] Thippareddy, K. S. and Agrawal, Pushpa (2010). Pre-treatment of Agriculture Waste using Aspergillus flavus for the Production of Ethanol by Saccharomyces cerevisiae Journal of Environmental Research and Development 5 (2): 393.
[21] Janani, K., Ketzi, M., Megavathi, S., Vinothkumar, D., Ramesh Babu, N. G. (2013). Comparative Studies of Ethanol Production from Different Fruit Wastes Using Saccharomyces cerevisiae. International Journal of Innovative Research in Science, Journal Engineering and Technology 2 (12): 7161-7167.
[22] Neelakandan, T. and Usharani, G. (2009). Optimization and Production of Bioethanol from Cashew Apple Juice Using Immobilized Yeast Cells by Saccharomyces cerevisiae. American- Eurasian Journal of Scientific Research 4 (2): 85-88.
[23] Isono, Y., and Hoshino, A., (2000). Production of Ethanol using Granulated Yeast Cells Prepared by a Spray Dryer. Journal of General Applied Microbiology. 46: 231-234.
Cite This Article
  • APA Style

    Abdulkadir Adedeji Shittu, Orukotan Abimbola Ayodeji, Mohammed Sani Sambo Datsugwai. (2016). Bioethanol Production from Rice Winery Cake Using Lactic Acid Bacteria and Yeasts by the Process of Simultaneous Saccharification and Fermentation. International Journal of Microbiology and Biotechnology, 1(1), 33-39. https://doi.org/10.11648/j.ijmb.20160101.15

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    ACS Style

    Abdulkadir Adedeji Shittu; Orukotan Abimbola Ayodeji; Mohammed Sani Sambo Datsugwai. Bioethanol Production from Rice Winery Cake Using Lactic Acid Bacteria and Yeasts by the Process of Simultaneous Saccharification and Fermentation. Int. J. Microbiol. Biotechnol. 2016, 1(1), 33-39. doi: 10.11648/j.ijmb.20160101.15

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    AMA Style

    Abdulkadir Adedeji Shittu, Orukotan Abimbola Ayodeji, Mohammed Sani Sambo Datsugwai. Bioethanol Production from Rice Winery Cake Using Lactic Acid Bacteria and Yeasts by the Process of Simultaneous Saccharification and Fermentation. Int J Microbiol Biotechnol. 2016;1(1):33-39. doi: 10.11648/j.ijmb.20160101.15

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  • @article{10.11648/j.ijmb.20160101.15,
      author = {Abdulkadir Adedeji Shittu and Orukotan Abimbola Ayodeji and Mohammed Sani Sambo Datsugwai},
      title = {Bioethanol Production from Rice Winery Cake Using Lactic Acid Bacteria and Yeasts by the Process of Simultaneous Saccharification and Fermentation},
      journal = {International Journal of Microbiology and Biotechnology},
      volume = {1},
      number = {1},
      pages = {33-39},
      doi = {10.11648/j.ijmb.20160101.15},
      url = {https://doi.org/10.11648/j.ijmb.20160101.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijmb.20160101.15},
      abstract = {Bioethanol is a sustainable energy source which serves as an alternative to fossil fuel and contributes to a clean environment. Bioethanol was produced by individual activities of lactic acid bacteria and yeasts from rice cake waste using a simultaneous saccharification and fermentation process. The rice cake waste is a filtered solid waste of fermented rice wine mash and contains 78.04% of total carbohydrate, 10.88% of protein, 2.26% of ash, 8.12% of moisture and 0.7% of fat. The rice cake was mixed with raw starch digesting enzyme of Aspergillus niger and (Lactobacillus fermentum, Rhodotorula minuta, Rhodotorula mucilagnosa, Candida krusei, Kodamara ohmeri) respectively into different fermenting chambers. Rhodotorula minuta produced the highest efficiency of ethanol of 52.06% at the temperature of 30°C and pH of 2.58. Reducing sugar was observed to decrease with increase in bioethanol production and cell growth increased as the fermentation time increases. Bioethanol can also be produced from rice cake waste of a fermented rice mash, which can serve as a bio fuel and contributes to a healthy environment.},
     year = {2016}
    }
    

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  • TY  - JOUR
    T1  - Bioethanol Production from Rice Winery Cake Using Lactic Acid Bacteria and Yeasts by the Process of Simultaneous Saccharification and Fermentation
    AU  - Abdulkadir Adedeji Shittu
    AU  - Orukotan Abimbola Ayodeji
    AU  - Mohammed Sani Sambo Datsugwai
    Y1  - 2016/12/27
    PY  - 2016
    N1  - https://doi.org/10.11648/j.ijmb.20160101.15
    DO  - 10.11648/j.ijmb.20160101.15
    T2  - International Journal of Microbiology and Biotechnology
    JF  - International Journal of Microbiology and Biotechnology
    JO  - International Journal of Microbiology and Biotechnology
    SP  - 33
    EP  - 39
    PB  - Science Publishing Group
    SN  - 2578-9686
    UR  - https://doi.org/10.11648/j.ijmb.20160101.15
    AB  - Bioethanol is a sustainable energy source which serves as an alternative to fossil fuel and contributes to a clean environment. Bioethanol was produced by individual activities of lactic acid bacteria and yeasts from rice cake waste using a simultaneous saccharification and fermentation process. The rice cake waste is a filtered solid waste of fermented rice wine mash and contains 78.04% of total carbohydrate, 10.88% of protein, 2.26% of ash, 8.12% of moisture and 0.7% of fat. The rice cake was mixed with raw starch digesting enzyme of Aspergillus niger and (Lactobacillus fermentum, Rhodotorula minuta, Rhodotorula mucilagnosa, Candida krusei, Kodamara ohmeri) respectively into different fermenting chambers. Rhodotorula minuta produced the highest efficiency of ethanol of 52.06% at the temperature of 30°C and pH of 2.58. Reducing sugar was observed to decrease with increase in bioethanol production and cell growth increased as the fermentation time increases. Bioethanol can also be produced from rice cake waste of a fermented rice mash, which can serve as a bio fuel and contributes to a healthy environment.
    VL  - 1
    IS  - 1
    ER  - 

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Author Information
  • Department of Microbiology, Faculty of Science, Kaduna State University, Kaduna, Nigeria

  • Department of Microbiology, Faculty of Science, Kaduna State University, Kaduna, Nigeria

  • Department of Microbiology, Faculty of Science, Kaduna State University, Kaduna, Nigeria

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