Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection

Khandaker Shadia; S. M. Mostofa Kamal; Ahmed Abu Saleh; Mohammed Nayem Hossain; Ratan Das Gupta; Md. Ruhul Amin Miah

doi:doi:10.11648/j.ajbls.20150303.14

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Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection

Khandaker Shadia, S. M. Mostofa Kamal, Ahmed Abu Saleh, Mohammed Nayem Hossain, Ratan Das Gupta, Md. Ruhul Amin Miah

Published in American Journal of Biomedical and Life Sciences (Volume 3, Issue 3)

Received: 16 March 2015 Accepted: 31 March 2015 Published: 30 April 2015

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Abstract

Diagnosis of tuberculosis from different body fluids remains challenging due to various limitations of the conventional and molecular methods. We studied the role of adenosine deaminase (ADA) assay to diagnose tubercular infection in cerebrospinal fluid, peritoneal fluid and pleural fluid. Fifty three patients with tubercular meningitis, peritonitis and pleuritis were enrolled in this study on the basis of clinical, radiological, cytological, biochemical and somewhere bacteriological evidences. Cases positive by AFB smear, culture or PCR were considered as confirmed TB and other as probable TB cases. Another 28 non-TB cases were included as control. In 53 suspected TB cases ADA was found positive in highest 42 (79.2%) cases, whereas smear and/ culture in 10 (18.7%) and PCR in 18 (33.9%) cases. ADA assay revealed 100% positivity in confirmed TB cases and 14.3% in non TB cases. The sensitivity and specificity of ADA was found 79% and 86% respectively when the cut off value was used ≥ 10 IU/L for CSF and ≥ 40 IU/L for pleural or peritoneal fluid. Mean ADA values of confirmed and probable TB cases were found significantly higher than that of non TB cases (< 0.05). Significance of difference was determined by ANOVA and Kruskal-Wallis test. Thus, adenosine deaminase assay in body fluids has proved to be efficient, reliable and simple method to diagnose tubercular meningitis, peritonitis and pleuritis.

Published in	American Journal of Biomedical and Life Sciences (Volume 3, Issue 3)
DOI	10.11648/j.ajbls.20150303.14
Page(s)	49-54
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Body Fluid, Tuberculosis, Adenosine Deaminase Assay

References

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APA Style

Khandaker Shadia, S. M. Mostofa Kamal, Ahmed Abu Saleh, Mohammed Nayem Hossain, Ratan Das Gupta, et al. (2015). Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection. American Journal of Biomedical and Life Sciences, 3(3), 49-54. https://doi.org/10.11648/j.ajbls.20150303.14

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ACS Style

Khandaker Shadia; S. M. Mostofa Kamal; Ahmed Abu Saleh; Mohammed Nayem Hossain; Ratan Das Gupta, et al. Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection. Am. J. Biomed. Life Sci. 2015, 3(3), 49-54. doi: 10.11648/j.ajbls.20150303.14

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AMA Style

Khandaker Shadia, S. M. Mostofa Kamal, Ahmed Abu Saleh, Mohammed Nayem Hossain, Ratan Das Gupta, et al. Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection. Am J Biomed Life Sci. 2015;3(3):49-54. doi: 10.11648/j.ajbls.20150303.14

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@article{10.11648/j.ajbls.20150303.14,
  author = {Khandaker Shadia and S. M. Mostofa Kamal and Ahmed Abu Saleh and Mohammed Nayem Hossain and Ratan Das Gupta and Md. Ruhul Amin Miah},
  title = {Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {3},
  number = {3},
  pages = {49-54},
  doi = {10.11648/j.ajbls.20150303.14},
  url = {https://doi.org/10.11648/j.ajbls.20150303.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20150303.14},
  abstract = {Diagnosis of tuberculosis from different body fluids remains challenging due to various limitations of the conventional and molecular methods. We studied the role of adenosine deaminase (ADA) assay to diagnose tubercular infection in cerebrospinal fluid, peritoneal fluid and pleural fluid. Fifty three patients with tubercular meningitis, peritonitis and pleuritis were enrolled in this study on the basis of clinical, radiological, cytological, biochemical and somewhere bacteriological evidences. Cases positive by AFB smear, culture or PCR were considered as confirmed TB and other as probable TB cases. Another 28 non-TB cases were included as control. In 53 suspected TB cases ADA was found positive in highest 42 (79.2%) cases, whereas smear and/ culture in 10 (18.7%) and PCR in 18 (33.9%) cases. ADA assay revealed 100% positivity in confirmed TB cases and 14.3% in non TB cases. The sensitivity and specificity of ADA was found 79% and 86% respectively when the cut off value was used ≥ 10 IU/L for CSF and ≥ 40 IU/L for pleural or peritoneal fluid. Mean ADA values of confirmed and probable TB cases were found significantly higher than that of non TB cases (< 0.05). Significance of difference was determined by ANOVA and Kruskal-Wallis test. Thus, adenosine deaminase assay in body fluids has proved to be efficient, reliable and simple method to diagnose tubercular meningitis, peritonitis and pleuritis.},
 year = {2015}
}

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TY - JOUR
T1 - Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection
AU - Khandaker Shadia
AU - S. M. Mostofa Kamal
AU - Ahmed Abu Saleh
AU - Mohammed Nayem Hossain
AU - Ratan Das Gupta
AU - Md. Ruhul Amin Miah
Y1 - 2015/04/30
PY - 2015
N1 - https://doi.org/10.11648/j.ajbls.20150303.14
DO - 10.11648/j.ajbls.20150303.14
T2 - American Journal of Biomedical and Life Sciences
JF - American Journal of Biomedical and Life Sciences
JO - American Journal of Biomedical and Life Sciences
SP - 49
EP - 54
PB - Science Publishing Group
SN - 2330-880X
UR - https://doi.org/10.11648/j.ajbls.20150303.14
AB - Diagnosis of tuberculosis from different body fluids remains challenging due to various limitations of the conventional and molecular methods. We studied the role of adenosine deaminase (ADA) assay to diagnose tubercular infection in cerebrospinal fluid, peritoneal fluid and pleural fluid. Fifty three patients with tubercular meningitis, peritonitis and pleuritis were enrolled in this study on the basis of clinical, radiological, cytological, biochemical and somewhere bacteriological evidences. Cases positive by AFB smear, culture or PCR were considered as confirmed TB and other as probable TB cases. Another 28 non-TB cases were included as control. In 53 suspected TB cases ADA was found positive in highest 42 (79.2%) cases, whereas smear and/ culture in 10 (18.7%) and PCR in 18 (33.9%) cases. ADA assay revealed 100% positivity in confirmed TB cases and 14.3% in non TB cases. The sensitivity and specificity of ADA was found 79% and 86% respectively when the cut off value was used ≥ 10 IU/L for CSF and ≥ 40 IU/L for pleural or peritoneal fluid. Mean ADA values of confirmed and probable TB cases were found significantly higher than that of non TB cases (< 0.05). Significance of difference was determined by ANOVA and Kruskal-Wallis test. Thus, adenosine deaminase assay in body fluids has proved to be efficient, reliable and simple method to diagnose tubercular meningitis, peritonitis and pleuritis.
VL - 3
IS - 3
ER -

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Author Information

Khandaker Shadia

Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh
S. M. Mostofa Kamal

National Tuberculosis Reference Laboratory, Institute of Diseases of the Chest and Hospital (NIDCH), Dhaka, Bangladesh
Ahmed Abu Saleh

Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh
Mohammed Nayem Hossain

Department of Surgery, Dhaka Medical College Hospital, Dhaka, Bangladesh
Ratan Das Gupta

Department of Medicine, Dhaka Medical College Hospital, Dhaka, Bangladesh
Md. Ruhul Amin Miah

Department of Microbiology and Immunology, Bangabandhu Sheikh Mujib Medical University, Dhaka, Bangladesh

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APA Style

Khandaker Shadia, S. M. Mostofa Kamal, Ahmed Abu Saleh, Mohammed Nayem Hossain, Ratan Das Gupta, et al. (2015). Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection. American Journal of Biomedical and Life Sciences, 3(3), 49-54. https://doi.org/10.11648/j.ajbls.20150303.14

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Khandaker Shadia; S. M. Mostofa Kamal; Ahmed Abu Saleh; Mohammed Nayem Hossain; Ratan Das Gupta, et al. Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection. Am. J. Biomed. Life Sci. 2015, 3(3), 49-54. doi: 10.11648/j.ajbls.20150303.14

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AMA Style

Khandaker Shadia, S. M. Mostofa Kamal, Ahmed Abu Saleh, Mohammed Nayem Hossain, Ratan Das Gupta, et al. Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection. Am J Biomed Life Sci. 2015;3(3):49-54. doi: 10.11648/j.ajbls.20150303.14

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@article{10.11648/j.ajbls.20150303.14,
  author = {Khandaker Shadia and S. M. Mostofa Kamal and Ahmed Abu Saleh and Mohammed Nayem Hossain and Ratan Das Gupta and Md. Ruhul Amin Miah},
  title = {Adenosine Deaminase Assay in Different Body Fluids for the Diagnosis of Tubercular Infection},
  journal = {American Journal of Biomedical and Life Sciences},
  volume = {3},
  number = {3},
  pages = {49-54},
  doi = {10.11648/j.ajbls.20150303.14},
  url = {https://doi.org/10.11648/j.ajbls.20150303.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20150303.14},
  abstract = {Diagnosis of tuberculosis from different body fluids remains challenging due to various limitations of the conventional and molecular methods. We studied the role of adenosine deaminase (ADA) assay to diagnose tubercular infection in cerebrospinal fluid, peritoneal fluid and pleural fluid. Fifty three patients with tubercular meningitis, peritonitis and pleuritis were enrolled in this study on the basis of clinical, radiological, cytological, biochemical and somewhere bacteriological evidences. Cases positive by AFB smear, culture or PCR were considered as confirmed TB and other as probable TB cases. Another 28 non-TB cases were included as control. In 53 suspected TB cases ADA was found positive in highest 42 (79.2%) cases, whereas smear and/ culture in 10 (18.7%) and PCR in 18 (33.9%) cases. ADA assay revealed 100% positivity in confirmed TB cases and 14.3% in non TB cases. The sensitivity and specificity of ADA was found 79% and 86% respectively when the cut off value was used ≥ 10 IU/L for CSF and ≥ 40 IU/L for pleural or peritoneal fluid. Mean ADA values of confirmed and probable TB cases were found significantly higher than that of non TB cases (< 0.05). Significance of difference was determined by ANOVA and Kruskal-Wallis test. Thus, adenosine deaminase assay in body fluids has proved to be efficient, reliable and simple method to diagnose tubercular meningitis, peritonitis and pleuritis.},
 year = {2015}
}

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