The rapid rise in resistance of Staphylococcus aureus to various antimicrobial agents is now a growing concern. The objectives of this study were to investigate the frequency of Methicillin Resistant S. aureus (MRSA) in different cases of infection, and to determine the pattern of antimicrobial susceptibility among the S. aureus originated from human in Mymensingh, Bangladesh. Covering a period of five months during July 2014 to November 2014, a total of 65 samples comprises of pus, wounds, eye lesions, burns were collected from the Community Based Medical College Hospital (CBMCH), Mymensingh, Bangladesh, and the samples were processed at the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh-2202. Among the 65 samples, 20% (n=13/65) were confirmed as S. aureus on the basis of colony morphology, staining characteristics, biochemical properties, and Polymerase Chain Reaction (PCR) by amplification of nuc gene (amplicon size 279-bp). Among these 13 S. aureus isolates, 15.38% (n=2/13) were found to be resistant to Methicillin which was confirmed by antibiotic disk sensitivity test and PCR by amplifying MRSA specific mecA gene (amplicon size 533-bp). The antibiotic disk sensitivity test indicated that the MRSA isolates were completely (100%) resistant to Penicillin and Erythromycin. The MRSA represents a major challenge for treatment in hospital due to the emergence and spread of antibiotic resistant isolates among susceptible individual.
| Published in | American Journal of Biomedical and Life Sciences (Volume 4, Issue 3) |
| DOI | 10.11648/j.ajbls.20160403.11 |
| Page(s) | 26-29 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
nuc Gene, mecA Gene, MRSA, Human, Vancomycin
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APA Style
Khalid Hussain, Marzia Rahman, K. H. M. Nazmul Hussain Nazir, Hasibur Rahman, Abul Khair. (2016). Methicillin Resistant Staphylococcus aureus (MRSA) in Patients of Community Based Medical College Hospital, Mymensingh, Bangladesh. American Journal of Biomedical and Life Sciences, 4(3), 26-29. https://doi.org/10.11648/j.ajbls.20160403.11
ACS Style
Khalid Hussain; Marzia Rahman; K. H. M. Nazmul Hussain Nazir; Hasibur Rahman; Abul Khair. Methicillin Resistant Staphylococcus aureus (MRSA) in Patients of Community Based Medical College Hospital, Mymensingh, Bangladesh. Am. J. Biomed. Life Sci. 2016, 4(3), 26-29. doi: 10.11648/j.ajbls.20160403.11
AMA Style
Khalid Hussain, Marzia Rahman, K. H. M. Nazmul Hussain Nazir, Hasibur Rahman, Abul Khair. Methicillin Resistant Staphylococcus aureus (MRSA) in Patients of Community Based Medical College Hospital, Mymensingh, Bangladesh. Am J Biomed Life Sci. 2016;4(3):26-29. doi: 10.11648/j.ajbls.20160403.11
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Download@article{10.11648/j.ajbls.20160403.11,
author = {Khalid Hussain and Marzia Rahman and K. H. M. Nazmul Hussain Nazir and Hasibur Rahman and Abul Khair},
title = {Methicillin Resistant Staphylococcus aureus (MRSA) in Patients of Community Based Medical College Hospital, Mymensingh, Bangladesh},
journal = {American Journal of Biomedical and Life Sciences},
volume = {4},
number = {3},
pages = {26-29},
doi = {10.11648/j.ajbls.20160403.11},
url = {https://doi.org/10.11648/j.ajbls.20160403.11},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ajbls.20160403.11},
abstract = {The rapid rise in resistance of Staphylococcus aureus to various antimicrobial agents is now a growing concern. The objectives of this study were to investigate the frequency of Methicillin Resistant S. aureus (MRSA) in different cases of infection, and to determine the pattern of antimicrobial susceptibility among the S. aureus originated from human in Mymensingh, Bangladesh. Covering a period of five months during July 2014 to November 2014, a total of 65 samples comprises of pus, wounds, eye lesions, burns were collected from the Community Based Medical College Hospital (CBMCH), Mymensingh, Bangladesh, and the samples were processed at the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh-2202. Among the 65 samples, 20% (n=13/65) were confirmed as S. aureus on the basis of colony morphology, staining characteristics, biochemical properties, and Polymerase Chain Reaction (PCR) by amplification of nuc gene (amplicon size 279-bp). Among these 13 S. aureus isolates, 15.38% (n=2/13) were found to be resistant to Methicillin which was confirmed by antibiotic disk sensitivity test and PCR by amplifying MRSA specific mecA gene (amplicon size 533-bp). The antibiotic disk sensitivity test indicated that the MRSA isolates were completely (100%) resistant to Penicillin and Erythromycin. The MRSA represents a major challenge for treatment in hospital due to the emergence and spread of antibiotic resistant isolates among susceptible individual.},
year = {2016}
}
TY - JOUR T1 - Methicillin Resistant Staphylococcus aureus (MRSA) in Patients of Community Based Medical College Hospital, Mymensingh, Bangladesh AU - Khalid Hussain AU - Marzia Rahman AU - K. H. M. Nazmul Hussain Nazir AU - Hasibur Rahman AU - Abul Khair Y1 - 2016/04/21 PY - 2016 N1 - https://doi.org/10.11648/j.ajbls.20160403.11 DO - 10.11648/j.ajbls.20160403.11 T2 - American Journal of Biomedical and Life Sciences JF - American Journal of Biomedical and Life Sciences JO - American Journal of Biomedical and Life Sciences SP - 26 EP - 29 PB - Science Publishing Group SN - 2330-880X UR - https://doi.org/10.11648/j.ajbls.20160403.11 AB - The rapid rise in resistance of Staphylococcus aureus to various antimicrobial agents is now a growing concern. The objectives of this study were to investigate the frequency of Methicillin Resistant S. aureus (MRSA) in different cases of infection, and to determine the pattern of antimicrobial susceptibility among the S. aureus originated from human in Mymensingh, Bangladesh. Covering a period of five months during July 2014 to November 2014, a total of 65 samples comprises of pus, wounds, eye lesions, burns were collected from the Community Based Medical College Hospital (CBMCH), Mymensingh, Bangladesh, and the samples were processed at the Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh-2202. Among the 65 samples, 20% (n=13/65) were confirmed as S. aureus on the basis of colony morphology, staining characteristics, biochemical properties, and Polymerase Chain Reaction (PCR) by amplification of nuc gene (amplicon size 279-bp). Among these 13 S. aureus isolates, 15.38% (n=2/13) were found to be resistant to Methicillin which was confirmed by antibiotic disk sensitivity test and PCR by amplifying MRSA specific mecA gene (amplicon size 533-bp). The antibiotic disk sensitivity test indicated that the MRSA isolates were completely (100%) resistant to Penicillin and Erythromycin. The MRSA represents a major challenge for treatment in hospital due to the emergence and spread of antibiotic resistant isolates among susceptible individual. VL - 4 IS - 3 ER -
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