Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic

Wai Prathumpai; Pranee Rachathewee; Sutamat Khajeeram; Jean-Jacques Sanglier; Pariyada Tanjak; Pawadee Methacanon

doi:doi:10.11648/j.ab.20130102.12

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Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic

Wai Prathumpai, Pranee Rachathewee, Sutamat Khajeeram, Jean-Jacques Sanglier, Pariyada Tanjak, Pawadee Methacanon

Published in Advances in Biochemistry (Volume 1, Issue 2)

Received: 5 March 2013 Accepted: Published: 20 June 2013

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Abstract

Optimization of exopolysaccharides (EPS) produced by three strains of entomopathogenic fungi (Beauveria bassiana BCC 2692, Ophiocordyceps dipterigena BCC 2073, and Paecilomyces tenuipes BCC 2656) was carried out together with analyses of their prebiotic properties. B. bassiana BCC 2692 produced 6.27±0.22 g/L EPS on optimal medium using two-level fractional factorial design and 4.7 g/L EPS in bioreactor. EPS productions of O. dipterigena BCC 2073 were 13.2 g/L and 41.2 g/L in shake flask and bioreactor, respectively. For P. tenuipes BCC 2656, 1.47±0.21 g/L EPS in shake flask and 28.1 g/L EPS in bioreactor were obtained. These EPS were previously characterized as -glucan with differences in molecular weights and degree of branching. They were resistant to hydrolysis by both hydrochloric acid and porcine pancreatic α-amylase. Furthermore, when used as the sole carbon source, all three types of EPS supported growth in vitro of two different probiotic bacteria (Lactobacillus acidoplilus BCC 13839 and Bifidobacterium animalis ATCC 25527). A constant viability of L. acidophilus BCC 13839 was maintained throughout the cultivation period (48 hours) on all three entomopatogenic fungal EPS. All EPS also supported better growth and maintained longer growth period of B. animalis ATCC 25527 than glucose or inulin. Thus these entomopathogenic fungi EPS are promising candidates in prebiotic industry, expanding the pool of current commercial prebiotics.

Published in	Advances in Biochemistry (Volume 1, Issue 2)
DOI	10.11648/j.ab.20130102.12
Page(s)	13-21
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Exopolysaccharide, Glucan, Prebiotic, Probiotic, Entomopathogenic Fungi

References

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Wai Prathumpai, Pranee Rachathewee, Sutamat Khajeeram, Jean-Jacques Sanglier, Pariyada Tanjak, et al. (2013). Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic. Advances in Biochemistry, 1(2), 13-21. https://doi.org/10.11648/j.ab.20130102.12

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Wai Prathumpai; Pranee Rachathewee; Sutamat Khajeeram; Jean-Jacques Sanglier; Pariyada Tanjak, et al. Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic. Adv. Biochem. 2013, 1(2), 13-21. doi: 10.11648/j.ab.20130102.12

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AMA Style

Wai Prathumpai, Pranee Rachathewee, Sutamat Khajeeram, Jean-Jacques Sanglier, Pariyada Tanjak, et al. Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic. Adv Biochem. 2013;1(2):13-21. doi: 10.11648/j.ab.20130102.12

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@article{10.11648/j.ab.20130102.12,
  author = {Wai Prathumpai and Pranee Rachathewee and Sutamat Khajeeram and Jean-Jacques Sanglier and Pariyada Tanjak and Pawadee Methacanon},
  title = {Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic},
  journal = {Advances in Biochemistry},
  volume = {1},
  number = {2},
  pages = {13-21},
  doi = {10.11648/j.ab.20130102.12},
  url = {https://doi.org/10.11648/j.ab.20130102.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20130102.12},
  abstract = {Optimization of exopolysaccharides (EPS) produced by three strains of entomopathogenic fungi (Beauveria bassiana BCC 2692, Ophiocordyceps dipterigena BCC 2073, and Paecilomyces tenuipes BCC 2656) was carried out together with analyses of their prebiotic properties. B. bassiana BCC 2692 produced 6.27±0.22 g/L EPS on optimal medium using two-level fractional factorial design and 4.7 g/L EPS in bioreactor. EPS productions of O. dipterigena BCC 2073 were 13.2 g/L and 41.2 g/L in shake flask and bioreactor, respectively. For P. tenuipes BCC 2656, 1.47±0.21 g/L EPS in shake flask and 28.1 g/L EPS in bioreactor were obtained. These EPS were previously characterized as -glucan with differences in molecular weights and degree of branching. They were resistant to hydrolysis by both hydrochloric acid and porcine pancreatic α-amylase. Furthermore, when used as the sole carbon source, all three types of EPS supported growth in vitro of two different probiotic bacteria (Lactobacillus acidoplilus BCC 13839 and Bifidobacterium animalis ATCC 25527). A constant viability of L. acidophilus BCC 13839 was maintained throughout the cultivation period (48 hours) on all three entomopatogenic fungal EPS. All EPS also supported better growth and maintained longer growth period of B. animalis ATCC 25527 than glucose or inulin. Thus these entomopathogenic fungi EPS are promising candidates in prebiotic industry, expanding the pool of current commercial prebiotics.},
 year = {2013}
}

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TY - JOUR
T1 - Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic
AU - Wai Prathumpai
AU - Pranee Rachathewee
AU - Sutamat Khajeeram
AU - Jean-Jacques Sanglier
AU - Pariyada Tanjak
AU - Pawadee Methacanon
Y1 - 2013/06/20
PY - 2013
N1 - https://doi.org/10.11648/j.ab.20130102.12
DO - 10.11648/j.ab.20130102.12
T2 - Advances in Biochemistry
JF - Advances in Biochemistry
JO - Advances in Biochemistry
SP - 13
EP - 21
PB - Science Publishing Group
SN - 2329-0862
UR - https://doi.org/10.11648/j.ab.20130102.12
AB - Optimization of exopolysaccharides (EPS) produced by three strains of entomopathogenic fungi (Beauveria bassiana BCC 2692, Ophiocordyceps dipterigena BCC 2073, and Paecilomyces tenuipes BCC 2656) was carried out together with analyses of their prebiotic properties. B. bassiana BCC 2692 produced 6.27±0.22 g/L EPS on optimal medium using two-level fractional factorial design and 4.7 g/L EPS in bioreactor. EPS productions of O. dipterigena BCC 2073 were 13.2 g/L and 41.2 g/L in shake flask and bioreactor, respectively. For P. tenuipes BCC 2656, 1.47±0.21 g/L EPS in shake flask and 28.1 g/L EPS in bioreactor were obtained. These EPS were previously characterized as -glucan with differences in molecular weights and degree of branching. They were resistant to hydrolysis by both hydrochloric acid and porcine pancreatic α-amylase. Furthermore, when used as the sole carbon source, all three types of EPS supported growth in vitro of two different probiotic bacteria (Lactobacillus acidoplilus BCC 13839 and Bifidobacterium animalis ATCC 25527). A constant viability of L. acidophilus BCC 13839 was maintained throughout the cultivation period (48 hours) on all three entomopatogenic fungal EPS. All EPS also supported better growth and maintained longer growth period of B. animalis ATCC 25527 than glucose or inulin. Thus these entomopathogenic fungi EPS are promising candidates in prebiotic industry, expanding the pool of current commercial prebiotics.
VL - 1
IS - 2
ER -

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Author Information

Wai Prathumpai

National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand
Pranee Rachathewee

National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency, 113 Thailand Science Park, Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand
Sutamat Khajeeram

Department of Biotechnology, Thammasat University, Klong Nueng, Klong Luang, Pathumthani 12120, Thailand
Jean-Jacques Sanglier

Novartis Institute for BioMedical Research Basel, Natural Products Unit, Novartis Pharma AG, Basel, Switzerland
Pariyada Tanjak

National Metal and Materials Technology Center (MTEC), 114 Thailand Science Park, Paholyothin Rd., Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand
Pawadee Methacanon

National Metal and Materials Technology Center (MTEC), 114 Thailand Science Park, Paholyothin Rd., Klong Nueng, Klong Luang, Pathum Thani 12120, Thailand

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Wai Prathumpai, Pranee Rachathewee, Sutamat Khajeeram, Jean-Jacques Sanglier, Pariyada Tanjak, et al. (2013). Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic. Advances in Biochemistry, 1(2), 13-21. https://doi.org/10.11648/j.ab.20130102.12

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Wai Prathumpai; Pranee Rachathewee; Sutamat Khajeeram; Jean-Jacques Sanglier; Pariyada Tanjak, et al. Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic. Adv. Biochem. 2013, 1(2), 13-21. doi: 10.11648/j.ab.20130102.12

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AMA Style

Wai Prathumpai, Pranee Rachathewee, Sutamat Khajeeram, Jean-Jacques Sanglier, Pariyada Tanjak, et al. Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic. Adv Biochem. 2013;1(2):13-21. doi: 10.11648/j.ab.20130102.12

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@article{10.11648/j.ab.20130102.12,
  author = {Wai Prathumpai and Pranee Rachathewee and Sutamat Khajeeram and Jean-Jacques Sanglier and Pariyada Tanjak and Pawadee Methacanon},
  title = {Optimization, Characterization and In Vitro Evaluation of Entomopathogenic Fungal Exopolysaccharides as Prebiotic},
  journal = {Advances in Biochemistry},
  volume = {1},
  number = {2},
  pages = {13-21},
  doi = {10.11648/j.ab.20130102.12},
  url = {https://doi.org/10.11648/j.ab.20130102.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20130102.12},
  abstract = {Optimization of exopolysaccharides (EPS) produced by three strains of entomopathogenic fungi (Beauveria bassiana BCC 2692, Ophiocordyceps dipterigena BCC 2073, and Paecilomyces tenuipes BCC 2656) was carried out together with analyses of their prebiotic properties. B. bassiana BCC 2692 produced 6.27±0.22 g/L EPS on optimal medium using two-level fractional factorial design and 4.7 g/L EPS in bioreactor. EPS productions of O. dipterigena BCC 2073 were 13.2 g/L and 41.2 g/L in shake flask and bioreactor, respectively. For P. tenuipes BCC 2656, 1.47±0.21 g/L EPS in shake flask and 28.1 g/L EPS in bioreactor were obtained. These EPS were previously characterized as -glucan with differences in molecular weights and degree of branching. They were resistant to hydrolysis by both hydrochloric acid and porcine pancreatic α-amylase. Furthermore, when used as the sole carbon source, all three types of EPS supported growth in vitro of two different probiotic bacteria (Lactobacillus acidoplilus BCC 13839 and Bifidobacterium animalis ATCC 25527). A constant viability of L. acidophilus BCC 13839 was maintained throughout the cultivation period (48 hours) on all three entomopatogenic fungal EPS. All EPS also supported better growth and maintained longer growth period of B. animalis ATCC 25527 than glucose or inulin. Thus these entomopathogenic fungi EPS are promising candidates in prebiotic industry, expanding the pool of current commercial prebiotics.},
 year = {2013}
}

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