The purified and thermo stable myrosinase from cauliflower seedlings was immobilized by 3.5% agar agar matrix and immobilized myrosinase chips were stored in extraction buffer. After 48 hours 30% enzyme activity was exuded into extraction media from immobilized enzyme chips. Effect of some metal ions and organic solvents on the activity of immobilized cauliflower myrosinase was studied. Amongst selective metal ions (Sr2+, Sn2+ and Ba2+), Sr2+ at 4 mM concentration exhibited marked activating effect on the activity up to 3 fold. However, Sn2+ and Ba2+ increased the activity to a certain extent and then suppressed. Activation kinetics of myrosinase in presence of Sn2+ and Sr2+ were studied between 0-20min.The rate of reaction was almost constant till 15 min and then slight deactivation was recorded at various concentrations used. On the other hand, few heavy metal ions [Fe2+, Fe3+, Cu2+ and Zn2+] strongly inhibited the activity even at lower concentrations. Several nonpolar organic solvents even at comparatively higher concentrations had detectable activation effects. Further, their activity was seen with respect totime (27 min). However, some protic polar organic solvents exhibited inhibitory effect with immobilized myrosinase except to butanol.
| Published in | Advances in Biochemistry (Volume 1, Issue 3) |
| DOI | 10.11648/j.ab.20130103.12 |
| Page(s) | 51-56 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Myrosinase, Cauliflower, Immobilization, Agar Agar, Metal Ions, Organic Solvent
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APA Style
Ajeet Kumar Rai, Om Prakash, Jagdish Singh, Prabhakar Mohan Singh. (2013). Immobilization of Cauliflower Myrosinase on Agar Agar Matrix and its Application with Various Effectors. Advances in Biochemistry, 1(3), 51-56. https://doi.org/10.11648/j.ab.20130103.12
ACS Style
Ajeet Kumar Rai; Om Prakash; Jagdish Singh; Prabhakar Mohan Singh. Immobilization of Cauliflower Myrosinase on Agar Agar Matrix and its Application with Various Effectors. Adv. Biochem. 2013, 1(3), 51-56. doi: 10.11648/j.ab.20130103.12
AMA Style
Ajeet Kumar Rai, Om Prakash, Jagdish Singh, Prabhakar Mohan Singh. Immobilization of Cauliflower Myrosinase on Agar Agar Matrix and its Application with Various Effectors. Adv Biochem. 2013;1(3):51-56. doi: 10.11648/j.ab.20130103.12
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Download@article{10.11648/j.ab.20130103.12,
author = {Ajeet Kumar Rai and Om Prakash and Jagdish Singh and Prabhakar Mohan Singh},
title = {Immobilization of Cauliflower Myrosinase on Agar Agar Matrix and its Application with Various Effectors},
journal = {Advances in Biochemistry},
volume = {1},
number = {3},
pages = {51-56},
doi = {10.11648/j.ab.20130103.12},
url = {https://doi.org/10.11648/j.ab.20130103.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20130103.12},
abstract = {The purified and thermo stable myrosinase from cauliflower seedlings was immobilized by 3.5% agar agar matrix and immobilized myrosinase chips were stored in extraction buffer. After 48 hours 30% enzyme activity was exuded into extraction media from immobilized enzyme chips. Effect of some metal ions and organic solvents on the activity of immobilized cauliflower myrosinase was studied. Amongst selective metal ions (Sr2+, Sn2+ and Ba2+), Sr2+ at 4 mM concentration exhibited marked activating effect on the activity up to 3 fold. However, Sn2+ and Ba2+ increased the activity to a certain extent and then suppressed. Activation kinetics of myrosinase in presence of Sn2+ and Sr2+ were studied between 0-20min.The rate of reaction was almost constant till 15 min and then slight deactivation was recorded at various concentrations used. On the other hand, few heavy metal ions [Fe2+, Fe3+, Cu2+ and Zn2+] strongly inhibited the activity even at lower concentrations. Several nonpolar organic solvents even at comparatively higher concentrations had detectable activation effects. Further, their activity was seen with respect totime (27 min). However, some protic polar organic solvents exhibited inhibitory effect with immobilized myrosinase except to butanol.},
year = {2013}
}
TY - JOUR T1 - Immobilization of Cauliflower Myrosinase on Agar Agar Matrix and its Application with Various Effectors AU - Ajeet Kumar Rai AU - Om Prakash AU - Jagdish Singh AU - Prabhakar Mohan Singh Y1 - 2013/08/30 PY - 2013 N1 - https://doi.org/10.11648/j.ab.20130103.12 DO - 10.11648/j.ab.20130103.12 T2 - Advances in Biochemistry JF - Advances in Biochemistry JO - Advances in Biochemistry SP - 51 EP - 56 PB - Science Publishing Group SN - 2329-0862 UR - https://doi.org/10.11648/j.ab.20130103.12 AB - The purified and thermo stable myrosinase from cauliflower seedlings was immobilized by 3.5% agar agar matrix and immobilized myrosinase chips were stored in extraction buffer. After 48 hours 30% enzyme activity was exuded into extraction media from immobilized enzyme chips. Effect of some metal ions and organic solvents on the activity of immobilized cauliflower myrosinase was studied. Amongst selective metal ions (Sr2+, Sn2+ and Ba2+), Sr2+ at 4 mM concentration exhibited marked activating effect on the activity up to 3 fold. However, Sn2+ and Ba2+ increased the activity to a certain extent and then suppressed. Activation kinetics of myrosinase in presence of Sn2+ and Sr2+ were studied between 0-20min.The rate of reaction was almost constant till 15 min and then slight deactivation was recorded at various concentrations used. On the other hand, few heavy metal ions [Fe2+, Fe3+, Cu2+ and Zn2+] strongly inhibited the activity even at lower concentrations. Several nonpolar organic solvents even at comparatively higher concentrations had detectable activation effects. Further, their activity was seen with respect totime (27 min). However, some protic polar organic solvents exhibited inhibitory effect with immobilized myrosinase except to butanol. VL - 1 IS - 3 ER -
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