Herbal Treatment of Scorpion Envenomation: Plant Extracts Inhibited Opisthacanthus Capensis Venom Phospholipase A2 Activity

Hassan Zanna; Sa’idu Ahmad; Babagana Abdulmalik; Magaji Tasi’u; Grace Okonkwo Abel; Hauwa Mustapha Musa

doi:doi:10.11648/j.ab.20140204.12

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Herbal Treatment of Scorpion Envenomation: Plant Extracts Inhibited Opisthacanthus Capensis Venom Phospholipase A2 Activity

Hassan Zanna, Sa’idu Ahmad, Babagana Abdulmalik, Magaji Tasi’u, Grace Okonkwo Abel, Hauwa Mustapha Musa

Published in Advances in Biochemistry (Volume 2, Issue 4)

Received: 28 July 2014 Accepted: 11 August 2014 Published: 20 August 2014

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Abstract

The inhibitory effects of Momordica charantia linn, Isoberlinia doka, Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L aqueous leaves extracts on Opisthacanthus capensis (Black creeping scorpion) venom phospholipase A2 (PLA2 ) activity was investigated. The enzyme from O. capensis venom had a pH and temperature optima of 5 and 60˚C respectively with an activation energy of 5.20 Kcal/mol. Different concentrations (4mg, 6mg and 8mg/ml) of Isoberlinia doka and Momordica charantia Linn inhibited the activity of O capensis venom PLA2 in vitro displaying an uncompetitive inhibition pattern with a decrease in the computed index of efficiency (Kcat). Different concentrations (4mg, 6mg and 8mg/ml) of Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L also inhibited O. capensis venom PLA2 activity in vitro but the inhibition pattern was competitive inhibition with Kcat remaining unchanged. This study reveals that the use of these plants by herbalists in northern Nigeria in the treatment of scorpion bites could be justifiable.

Published in	Advances in Biochemistry (Volume 2, Issue 4)
DOI	10.11648/j.ab.20140204.12
Page(s)	55-59
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Opisthacanthus Capensis, Venom, Phospholipase A2, Scorpion, Envenomation

References

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APA Style

Hassan Zanna, Sa’idu Ahmad, Babagana Abdulmalik, Magaji Tasi’u, Grace Okonkwo Abel, et al. (2014). Herbal Treatment of Scorpion Envenomation: Plant Extracts Inhibited Opisthacanthus Capensis Venom Phospholipase A2 Activity. Advances in Biochemistry, 2(4), 55-59. https://doi.org/10.11648/j.ab.20140204.12

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ACS Style

Hassan Zanna; Sa’idu Ahmad; Babagana Abdulmalik; Magaji Tasi’u; Grace Okonkwo Abel, et al. Herbal Treatment of Scorpion Envenomation: Plant Extracts Inhibited Opisthacanthus Capensis Venom Phospholipase A2 Activity. Adv. Biochem. 2014, 2(4), 55-59. doi: 10.11648/j.ab.20140204.12

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AMA Style

Hassan Zanna, Sa’idu Ahmad, Babagana Abdulmalik, Magaji Tasi’u, Grace Okonkwo Abel, et al. Herbal Treatment of Scorpion Envenomation: Plant Extracts Inhibited Opisthacanthus Capensis Venom Phospholipase A2 Activity. Adv Biochem. 2014;2(4):55-59. doi: 10.11648/j.ab.20140204.12

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@article{10.11648/j.ab.20140204.12,
  author = {Hassan Zanna and Sa’idu Ahmad and Babagana Abdulmalik and Magaji Tasi’u and Grace Okonkwo Abel and Hauwa Mustapha Musa},
  title = {Herbal Treatment of Scorpion Envenomation: Plant Extracts Inhibited Opisthacanthus Capensis Venom Phospholipase A2 Activity},
  journal = {Advances in Biochemistry},
  volume = {2},
  number = {4},
  pages = {55-59},
  doi = {10.11648/j.ab.20140204.12},
  url = {https://doi.org/10.11648/j.ab.20140204.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20140204.12},
  abstract = {The inhibitory effects of Momordica charantia linn, Isoberlinia doka, Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L aqueous leaves extracts on Opisthacanthus capensis (Black creeping scorpion) venom phospholipase A2 (PLA2 ) activity was investigated. The enzyme from O. capensis venom had a pH and temperature optima of 5 and 60˚C respectively with an activation energy of 5.20 Kcal/mol. Different concentrations (4mg, 6mg and 8mg/ml) of Isoberlinia  doka  and Momordica charantia  Linn inhibited the activity of O capensis venom PLA2 in vitro displaying an uncompetitive inhibition pattern with a decrease in the computed index of efficiency (Kcat). Different concentrations (4mg, 6mg and 8mg/ml) of Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L also inhibited O. capensis venom PLA2 activity in vitro but the inhibition pattern was competitive inhibition with Kcat remaining unchanged. This study reveals that the use of these plants by herbalists in northern Nigeria in the treatment of scorpion bites could be justifiable.},
 year = {2014}
}

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T1  - Herbal Treatment of Scorpion Envenomation: Plant Extracts Inhibited Opisthacanthus Capensis Venom Phospholipase A2 Activity
AU  - Hassan Zanna
AU  - Sa’idu Ahmad
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DO  - 10.11648/j.ab.20140204.12
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AB  - The inhibitory effects of Momordica charantia linn, Isoberlinia doka, Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L aqueous leaves extracts on Opisthacanthus capensis (Black creeping scorpion) venom phospholipase A2 (PLA2 ) activity was investigated. The enzyme from O. capensis venom had a pH and temperature optima of 5 and 60˚C respectively with an activation energy of 5.20 Kcal/mol. Different concentrations (4mg, 6mg and 8mg/ml) of Isoberlinia  doka  and Momordica charantia  Linn inhibited the activity of O capensis venom PLA2 in vitro displaying an uncompetitive inhibition pattern with a decrease in the computed index of efficiency (Kcat). Different concentrations (4mg, 6mg and 8mg/ml) of Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L also inhibited O. capensis venom PLA2 activity in vitro but the inhibition pattern was competitive inhibition with Kcat remaining unchanged. This study reveals that the use of these plants by herbalists in northern Nigeria in the treatment of scorpion bites could be justifiable.
VL  - 2
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Author Information

Hassan Zanna

Department of Biochemistry, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria
Sa’idu Ahmad

Department of Biochemistry, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria
Babagana Abdulmalik

Department of Biochemistry, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria
Magaji Tasi’u

Department of Biochemistry, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria
Grace Okonkwo Abel

Department of Biochemistry, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria
Hauwa Mustapha Musa

Department of Biochemistry, Faculty of Science, University of Maiduguri, Maiduguri, Nigeria

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Hassan Zanna, Sa’idu Ahmad, Babagana Abdulmalik, Magaji Tasi’u, Grace Okonkwo Abel, et al. (2014). Herbal Treatment of Scorpion Envenomation: Plant Extracts Inhibited Opisthacanthus Capensis Venom Phospholipase A2 Activity. Advances in Biochemistry, 2(4), 55-59. https://doi.org/10.11648/j.ab.20140204.12

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ACS Style

Hassan Zanna; Sa’idu Ahmad; Babagana Abdulmalik; Magaji Tasi’u; Grace Okonkwo Abel, et al. Herbal Treatment of Scorpion Envenomation: Plant Extracts Inhibited Opisthacanthus Capensis Venom Phospholipase A2 Activity. Adv. Biochem. 2014, 2(4), 55-59. doi: 10.11648/j.ab.20140204.12

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AMA Style

Hassan Zanna, Sa’idu Ahmad, Babagana Abdulmalik, Magaji Tasi’u, Grace Okonkwo Abel, et al. Herbal Treatment of Scorpion Envenomation: Plant Extracts Inhibited Opisthacanthus Capensis Venom Phospholipase A2 Activity. Adv Biochem. 2014;2(4):55-59. doi: 10.11648/j.ab.20140204.12

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@article{10.11648/j.ab.20140204.12,
  author = {Hassan Zanna and Sa’idu Ahmad and Babagana Abdulmalik and Magaji Tasi’u and Grace Okonkwo Abel and Hauwa Mustapha Musa},
  title = {Herbal Treatment of Scorpion Envenomation: Plant Extracts Inhibited Opisthacanthus Capensis Venom Phospholipase A2 Activity},
  journal = {Advances in Biochemistry},
  volume = {2},
  number = {4},
  pages = {55-59},
  doi = {10.11648/j.ab.20140204.12},
  url = {https://doi.org/10.11648/j.ab.20140204.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20140204.12},
  abstract = {The inhibitory effects of Momordica charantia linn, Isoberlinia doka, Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L aqueous leaves extracts on Opisthacanthus capensis (Black creeping scorpion) venom phospholipase A2 (PLA2 ) activity was investigated. The enzyme from O. capensis venom had a pH and temperature optima of 5 and 60˚C respectively with an activation energy of 5.20 Kcal/mol. Different concentrations (4mg, 6mg and 8mg/ml) of Isoberlinia  doka  and Momordica charantia  Linn inhibited the activity of O capensis venom PLA2 in vitro displaying an uncompetitive inhibition pattern with a decrease in the computed index of efficiency (Kcat). Different concentrations (4mg, 6mg and 8mg/ml) of Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L also inhibited O. capensis venom PLA2 activity in vitro but the inhibition pattern was competitive inhibition with Kcat remaining unchanged. This study reveals that the use of these plants by herbalists in northern Nigeria in the treatment of scorpion bites could be justifiable.},
 year = {2014}
}

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TY  - JOUR
T1  - Herbal Treatment of Scorpion Envenomation: Plant Extracts Inhibited Opisthacanthus Capensis Venom Phospholipase A2 Activity
AU  - Hassan Zanna
AU  - Sa’idu Ahmad
AU  - Babagana Abdulmalik
AU  - Magaji Tasi’u
AU  - Grace Okonkwo Abel
AU  - Hauwa Mustapha Musa
Y1  - 2014/08/20
PY  - 2014
N1  - https://doi.org/10.11648/j.ab.20140204.12
DO  - 10.11648/j.ab.20140204.12
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 55
EP  - 59
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20140204.12
AB  - The inhibitory effects of Momordica charantia linn, Isoberlinia doka, Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L aqueous leaves extracts on Opisthacanthus capensis (Black creeping scorpion) venom phospholipase A2 (PLA2 ) activity was investigated. The enzyme from O. capensis venom had a pH and temperature optima of 5 and 60˚C respectively with an activation energy of 5.20 Kcal/mol. Different concentrations (4mg, 6mg and 8mg/ml) of Isoberlinia  doka  and Momordica charantia  Linn inhibited the activity of O capensis venom PLA2 in vitro displaying an uncompetitive inhibition pattern with a decrease in the computed index of efficiency (Kcat). Different concentrations (4mg, 6mg and 8mg/ml) of Terminalia avicennioides, Tamarindus indica and Crotalaria retusa L also inhibited O. capensis venom PLA2 activity in vitro but the inhibition pattern was competitive inhibition with Kcat remaining unchanged. This study reveals that the use of these plants by herbalists in northern Nigeria in the treatment of scorpion bites could be justifiable.
VL  - 2
IS  - 4
ER  -

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