Hypno-Sedative Activity of Gymnema sylvestre Extract in Sodium Barbiturate - Induced Hypnosis in Rattus novergicus
Advances in Biochemistry
Volume 2, Issue 5, October 2014, Pages: 60-64
Received: Sep. 12, 2014;
Accepted: Sep. 19, 2014;
Published: Sep. 30, 2014
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Omale James, Department of Biochemistry, Faculty of Natural Sciences, Kogi State University, Anyigba, Nigeria
Menegbe Nasara Awam, Department of Biochemistry, Faculty of Natural Sciences, Kogi State University, Anyigba, Nigeria
Ojogbane Eleojo, Department of Biochemistry, Faculty of Natural Sciences, Kogi State University, Anyigba, Nigeria
Gymnema sylvestre is a rare herb with significant medical attributes and ethno-botanical uses. Insomnia is one of the most common sleep disorders around the world. In this study, the effect of the aqueous extracts of this plant was investigated for its hypno-sedative effect in rats as well as acute toxicity. Leaf, stem and flowers of G.sylvestre have several therapeutic applications in folk medicine in curing or managing of a wide range of diseases including insomnia. Sleep was induced using sodium barbiturate in albino rats. Rats of either sex weighing between 100-115g were grouped into five groups A,B,C,D and E of four animals each. Group A was the negative control and received only water, groups B the positive control received 10mg/kg b.w of diazepam .Groups C,D and E were administered 150,700 and 1500mg/kg b.w of extracts respectively, each of the rat was placed in individual cage and observed over a period of 24 hours at 6 hours interval .The aqueous extracts produced significant sedative effect at the dose of 150mg/kg b.w and was more effective than diazepam. The extracts at the doses of 150 and 700mg/kg b.w exhibited hypno-sedative activity comparable to the reference drug. The stem extract at 1500mg/kg produced no sedative effect. No toxicity signs were observed following acute toxicity evaluation. The extracts showed hypno-sedative activity at lower doses of 150mg/kg b.w in this increasing order, stem→leaf →flower. In conclusion, these results suggest that the aqueous extract of G. sylvestre is relatively non-toxic and possess potent sedative and hypnotic effects which could support its therapeutic use for insomnia in folk medicine.
Menegbe Nasara Awam,
Hypno-Sedative Activity of Gymnema sylvestre Extract in Sodium Barbiturate - Induced Hypnosis in Rattus novergicus, Advances in Biochemistry.
Vol. 2, No. 5,
2014, pp. 60-64.
Anis, M., Sharma, M.P., and lqbal M (2000) .Herbal ethno medicine of the Gwalior Forest division in Madhya Predesh India. Pharmaceutical Biology. 38.(4).241-253.
Benca, R. (2005) Diagnosis and treatment of chronic insomnia: A review. Psychiatric services. 56: 332-342.
Ch, B., Rao, K., Gandi, S., and Giri, A(2012). Abiotic elicitation of gymnemic acid in the suspension cultures of Gymnema sylvestre . World Journal of Microbiology and Biotechnology. 28(2):741-747.
Edinger, J., Means, M (2005) .Cognitive behavioral therapy for primary insomnia. Clinical Psychology Review. 256:539-558.
Gottesmann, C (2002) .GABA mechanisms and sleep. Neuroscience 111:321-239.
File, S.E and Wardill , A. G (1975). Validity of head dipping as a measure of exploration in modified hole board. Pschopharmacologia 44(1): 53- 59.
Foster, S (2002) .Gymnema sylvestre in alternative medicine review monograph. Thorne Research inc. 205-207.
Herrera-Ruiz, M., Gutierrez, C., Enrique Jimenez-ferrer, J., Tortoriello, J., Morion, G and Leon, I (2007) .Central nervous system depressant activity of an ethyl acetate extract from Ipomoea stans roots. Journal of ethno- pharmacology .112 (2) 243-247.
Harvey A. (2001). Insomnia: symptom or diagnosis? Clinical Psychology Review 21:1037-1059.
Huang, F., Xiong, Y., Xu, L., Ma, S,and Dou, C. (2007). Sedative and hypnotic activities of the ethanol fraction from Fructus schisandrae in mice and rats, Journal of ethnophamacology 110 (3): 471-475.
Kanetkar, P., Singhal R, and Kamat, M (2007) Gymnema sylvestre: a memoir’ Journal of Clinical Biochemistry and Nutrition 641(2): 77-81.
Kirtika, K.,R., Basu, B.D. (1975) Indian medicinal plants, vol.3 Periodical experts, Delhi india.
Litchfield, J.T and Wilcoxon, F (1949). A simplified method of evaluating dose effect experiments. Journal of Pharmacology and Experimental Therapeutics 96:99-113.
Nan, S.D., Mcrac, C.S., Cook, K.G, Lichstein, K.L. (2005). Treatment of insomnia in older adults. Clinical psychology review ,25:645-672.
Perez- Ortega, G., Guevara-Fefer, P., Chavez,M (2008). Sedative anxiolytic efficacy of Tilia americana var. mexicana inflorescences used traditionally by communities of state of Michoacan, mexizo. Journal of ethno pharmacology. 16 (3) 461-468.
Roy-Byrne , P.P( 2005). The GABA- benzodiazepine receptor complex : Structure, function , and role in anxiety . Journal of Clinical Psychiatry 66(2): 14 -20.
Wheatley, D. (2005) Medicinal plants for insomnia: A review of their pharmacology, efficacy and tolerability. Journal of Psychopharmacology. 19:414-421.
Brown EN, Lydic R, Schiff ND (2010). General anesthesia, sleep and coma. N Engl J Med. 363(27):2638–50.