Hydrolytic Enzyme Levels in Malted Cereals
Advances in Biochemistry
Volume 2, Issue 5, October 2014, Pages: 76-79
Received: Aug. 27, 2014; Accepted: Sep. 25, 2014; Published: Nov. 10, 2014
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Mohammed Adamu Milala, Biochemistry Department, Faculty of Science, University of Maiduguri, Borno State, Nigeria
Emmanuel Othumba Addy, Biochemistry Department, Faculty of Science, University of Maiduguri, Borno State, Nigeria
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Six grain samples namely barley, maize, millet, rice, sorghum and wheat were tested for enzyme levels (amylase, protease and lipase) in order to assess their malting characteristics, using barley as a standard. The levels of the enzymes were estimated in the whole dry grains, 24h steeped, 24h sprouted and 48h sprouted grains. A combined statistical analysis of linear regression and analysis of variance were used to test for enzyme interaction, similarities and interspecific relationship between the cereals. The estimated interspecific relationship between sorghum and barley was 84% based on the three enzymes, while millet had 75%. All the sprouted cereals with the exception of the standard barley had high lipase levels. This possibly suggests certain limitations as regards to production of off-flavours. Malt prepared from maize, sorghum, rice and millet had comparable protease levels with barley (P>0.05). The malts prepared from maize, millet, rice, sorghum and wheat had comparatively low alpha-amylase levels when compared with the standard barley. Multiple linear regressions showed amylase was dependent on lipase and protease. The relationship was not affected by either soaking (steeping) or sprouting of the grains. The implications of these findings suggest that a combination of these cereals might give good malt for food applications.
Amylase, Lipase, Protease, Malting, Cereals
To cite this article
Mohammed Adamu Milala, Emmanuel Othumba Addy, Hydrolytic Enzyme Levels in Malted Cereals, Advances in Biochemistry. Vol. 2, No. 5, 2014, pp. 76-79. doi: 10.11648/j.ab.20140205.14
Briggs, D.E. (1998). Malt and Malting. 1st Ed. Blackie Academic and Professional, London.
Ayenor, G.S and Ocloo, F.C.K. (2007). Physicochemical changes and diastase activity associated with germinating paddy rice (PSB. Rc 34). African Journal of food Science. 1; 037-041.S
Sripriya, G., Anthony, U. and Chandra, T.S. (1997). Changes in carbohydrate, free amino acids, organic acids, phytate and HCl extractability of minerals during germination and fermentation finger millet (Eleusine coracana)
Malleshi G and Desikachar M.S.R. (1986) Studies on the comparative malting characteristics of some tropical cereals and millets. Journal of the Institute of Brewing. 92; 174-176.
Novellie, L. (1977). Beverages from sorghum and millet for human food, May 11-12, 1976, Tropical Products Institute, Londin, pp: 73-77.
Mahesh G., Nissreen, A. and Eimear G. (2010). Barley for brewing: characteristic changes during malting, brewing and applications of its by-products. Comprehensive Rewiews in food Science and Food Safety. 9; 318-328.
Palmer, G.H.(1979). Adjuncts in brewing and distilling. Proceedings of Second Aviemore conference of the Institute of Brewing (Eds. Campbell, I. and Poist, F.G.) PP 24-45.
Mao, W.W. and Kinsella, J.E.(1981). Amylase activity in banana fruit, properties and changes in activity with ripening. Journal of Food Science 46: 1400.
Chaudhry, K. (1989). Biochemical Techniques. J.P. Brothers Medical Publishers New Delhi (India). Pp 145-149.
Plummer, D.T. (1978). An introduction to Practical Biochemistry (2nd edition) McGraw- Hill (UK) pp225-254.
Ladizinsky, G. and Hymowitz, T. (1979). Seed protein electrophoresis in Taxonomic and Evolution studies. Theoretical Applications of Genetics 54: 146-151.
Dewar, J., Taylor, J.R.N. and Berjak, p. (1997). Determination of improved steeping conditions for sorghum malting. Journal of Cereal Science. 26; 129-136.
Akoma, O., Onouha, S.A., Ajiboye, M.O., Akoma, A.O. and Alawoki, A.M. (2002). The nutritional and sensory quality characteristics of Kunun-zaki produced with the addition of hydrolytic enzymes from malted rice (Oryza sativa)
Palmer, G.H.(1989). Cereals in malting and brewing. Journal of Cereal Science Technology 17: 312-367
Palmer, G.H.(1985). Influence of Cell wall structure in enzymic breakdown of the endosperm of germinated barley. Journal of the Institute of Brewing. 47: 461-470.
Koleoso, A.O. and Olatunji, O.O. (1992). Sorghum malt/Adjunct replacement in clear (lager) beer: policy and practice in Nigeria: Utilization of sorghum and malt ( Gomez, M.J., House, L.R. Rooney, L.W. and Dendy, D.A.V edn) Patancheru, India pp 41-45
Palmer, G.H. and Shirakashi, Y.H. (1985). Influence of enzymic distribution on endosperm breakdown (modification) during malting. Journal of the American Society of Brewing Chemists, 43: 17-28.
Dendy, D.A.V.(1992). Composite flour, Past, Present and Future: A review with special emphasis on the place of composite flour in the Semi-arid zones: In utilization of sorghum and millet (Gomez, M.I., House, L.R. Rooney, L.W and D.A.V edn) ICRISAT India pp 67-73
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