Effects of Shea Butter Based Diet on Hepatic and Renal Enzymes and Plasma Lipid Profile in Albino Rats
Advances in Biochemistry
Volume 2, Issue 5, October 2014, Pages: 80-84
Received: Oct. 24, 2014; Accepted: Nov. 11, 2014; Published: Nov. 20, 2014
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Malachi Oluwaseyi Israel, Department of Biochemistry, Faculty of Science, Ekiti State University, Ado-Ekiti, Nigeria
Ajayi Olubunmi Bolanle, Department of Biochemistry, Faculty of Science, Ekiti State University, Ado-Ekiti, Nigeria
Akomolafe Seun Funmiola, Department of Biochemistry, Faculty of Science, Ekiti State University, Ado-Ekiti, Nigeria
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The effect of feeding Shea butter based diet on plasma, liver and kidney enzymes as well as the plasma lipid profile was studied. Twenty one weaned male rats weighing 35g to 45g were divided into three groups: control, test one and test two, each containing seven rats. Control group was given feed containing soya bean oil as lipid source ad libitum. In test groups one and two, Shea butter in 5% and 15% (w/w) respectively, replaced soya bean oil. The feeding lasted for 28 days after which the rats were sacrificed and the plasma as well as tissue samples from liver and kidney were collected. From the plasma, lipid profile; aspartate and alanine aminotransferases, alkaline phosphatase and total protein were assayed. From the tissue samples, aspartate and alanine aminotransferases, alkaline phosphatase and total protein were assayed. Significant decrease (P < 0.05) was observed in the total cholesterol (TC), high density lipoprotein (HDL), low density lipoprotein (LDL) and Triglyceride (TG) upon feeding with Shea butter based diet. Feeding with Shea butter did not pose any threat to hepatic and renal tissues.
Shea Butter, Renal, Hepatic, Lipid Profile
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Malachi Oluwaseyi Israel, Ajayi Olubunmi Bolanle, Akomolafe Seun Funmiola, Effects of Shea Butter Based Diet on Hepatic and Renal Enzymes and Plasma Lipid Profile in Albino Rats, Advances in Biochemistry. Vol. 2, No. 5, 2014, pp. 80-84. doi: 10.11648/j.ab.20140205.15
Alfred T. "Fats and Fatty Oils". Ullmann's Encyclopedia of Industrial Chemistry. Weinheim: Wiley-VCH. 2002
Samuel H. American Shea Butter Institute hand book, 11.28 http://www.American_Shea institute.com/ 2005.
Ndukwe I. G., Amupitan J.O. Isah Y. and Adegoke K. S. Phytochemical screening of the crude extract of the roots, stem bark and leaves of Vitallaria paradoxa (GAERTN. F). Afr J Biotechnol 2007; 6(16) pp 1905-1909.
Pretaorius C. J. and Watt E. Purification and Identification of Active components of Carpobrotus edullis L. J. Enthnopharma 2001; 76: 87-91.
Agbahungba G. and Depommier D. World Oil Seeds Chemistry, Technology and Utilization. Van Nostraud Rein Hold, New York. p.554, 1989.
Soladoye M. O., Orhiere S. S. and Ibimode B. M. Ethanobotanical Study of two Indigenous Multipurpose Plants in the Guinea Savanna of Kwara State –Vitellaria paradoxa and Parkia biglobosa. Biennial Conference of Ecological Society of Nigeria. Forestry Research Institute, Ibadan. p.13, 1989.
National Research Council. Lost Crops of Africa: Volume II: Vegetables. 2006 ISBN 978-0-309-10333-6.
Akihisa T., Kojima N., Katoh N., Ichimura Y., Suzuki H., Fukatsu M., Maranz S. and Masters E. T. Triterpene alcohol and fatty acid composition of Shea nut from seven African countries. J Oleo Sci. 2010; 59(7): 351-360.
Ogbonnaya C. and AdgidiziP. P. Evaluation of somePhysico-chemical properties of Shea butter (Butyrospermum paradoxum) related to its value for food and industrial utilization. Intl J Post Harv Technol & Innov. 2008; 1(3) pp 320-326.
Fold N. "A matter of good taste? Quality and the construction of standards for chocolate in the European Union. Cahiers d’Economieet Sociologie Rurales. 2000; 55/56: 92–110"
Alander J. Shea Butter- a Multi Functional Ingredient for Food and Cosmetics. Lipid Technol. 2004; 16:202-205.
Esuoso K.O., Lutz H., Bayer E. and Kutubuddin M. Unsaponifiable Lipid Constituents of Some Underutilized Tropical Seed Oils. J. Agric. Food Chem. 2000; 48: 231-234.
Essengue S. B., Stechschulte D. and Olson N. The use of Shea butter as an Emollient for Eczema. J Allergy & Clin Immunol. 2009; 123(2). Pp 145-148.
Kanwaljit K. C., Ramon B., Rosalia A., Galina G. and Anna N. W. Shea butter contains no IgE-binding soluble proteins. Letter to the editor. J Allergy Clin Immunol. 2010; 127( 3) pp 680–682. doi:10.1016/j.jaci.2010.10.022.
Masters E. T., Yidana J. A. and Lovett P. N. Reinforcing Sound Management through Trade: Shea Tree Products in Africa. Unasylva. 2004; 210: 46-52.
Akinwale A., Modu S., Maisartu M.A., Zainab M. A. and Bilkisu U. M. A. Effect of Feeding Various Concentrations of Shea oil on Some Biochemical Parameters in Normal Albino Rat. Bull Environ Pharmacol & Life Sc. 2012; 1(2), pp14 -17. URL: www.bepls.com Online ISSN 2277-1808.
Steins E. A. and Meyers G. L. National cholesterol education programme: Recommendations for triglyceride measurements, Clin Chem. 1995; 41: 1421-1426.
Friedewald W., Levy R. and Fredrickson D. ().Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultra-centrifuge, Clin. Chem. 1972; 18: 499-502.
Elizalde G. and Scalfani A. Fat appetite in rats: flavor preferences conditioned by nutritive and non-nutritive oil emulsions. Appetite. 1990; 15(3):189-197. PMID 2281949.
Ackroff K., Vigorito M. and Sclafani A. Fat appetite in rats: the response of infant and adult rats to nutritive and non-nutritive oil emulsions. Appetite. 1990; 15(3):171-188.
Jain K. S., Kathiravan M. K., Somani R. S., and Shishoo C. J. The biology and chemistry of hyperlipidemia. Bio. Med. Chem., 2007; 15: 4674-4699.
Kinosian B., Glick H., Preiss L., and Puder K. L. Cholesterol and coronary heart disease: predicting risks in men by changes in levels and ratios. J of Inves Med., 1995; 43:443–450.
Panagiotakos D. B., Pitsavos C., Skoumas J., Chrysohoou C., Toutouza M., Stefanadis C. L. and Toutouza P. K. Importance of LDL/HDL cholesterol ratio as a predictor for coronary heart disease events in patients with heterozygous familial hypercholesterolemia: a 15-year follow-up (1987-2002). Curr Med Res Opin; 2003; 19(2): 89 –94.
Natarajan S., Glick H., Criqui M., Horowitz D., Lipsitz S. R., and Kinosian B. Cholesterol measures to identify and treat individuals at risk for coronary heart disease. Am J Prev Med., 2003; 25: 50–57.
El-Mahmood A. M., Doughari J. H. and Ladan N. Antimicrobial screening of stem bark extracts of Vitellaria paradoxa against some enteric pathogenic microorganisms. Afr J Pharm Pharmacol 2008; 2(5). pp. 089-094.
Das T. K., Banerjee D., Charkraborty D., Pakhira M. C., Shrivastava B. and Kuhad R. C. Saponin: Role in animal system. Vet. World. 2012; 5(4):248-254.
Sidhu G. S. and Oakenfull D. G. A mechanism for the hypercholesterolemic effect of saponins. Br. J. Nutr. 2005; 55(3):643-649.
Oakenfull D. G. and Sidhu G. S. Could saponins be useful treatment for hypercholesterolemia? Eur J Clin Nutri. 1990; 44: 79-88
Yuldasheva L. N., Carvalho E. B., Catanho T. J. and Krasilnikov O. V. Cholesterol-dependent haemolytic activity of Passiflora quadrangularis leaves. Braz. J. Med. Biol. Res. 2005; 38(7) 1061-1070.
Matsuura M. Saponins in garlic as modifiers of the risk of cardiovascular disease. J Nutri. 2001; 131:1000S-1005S.
Han L.K., Xu B.J., Kimura Y., Zheng Y.N. and Okuda H. Platycodiradix affects lipid metabolism in mice with high fat diet induced obesity. J Nutr. 2000; 130:2760-2764.
Davrieux F., Allal F., Plombo G., Kelly B., Okulo J. B., Thiam M., Diallo O. B. and Bouvet J. M. Near infrared spectroscopy for High-Throughput characterization of Shea tree (Vitellaria paradoxa) nut fat profiles. J agric food chem. 2010; 58, 7811-7819.
Pronczuk A., Khosla P. and Hayes K. C. Dietary myristic, palmitic, and linoleic acids modulate cholesterolemia in gerbils. Foster Biomedical Research Laboratory, Brandeis University, Waltham, Massachusetts 02254. FASEB J. 1994; 8(14):1191-200.
Labat J. B., Martini M. C., Carr T. P., Elhard B. M., Olson B. A., Bergmann S. D., Slavin J. L., Hayes K. C. and Hassel C. A. Cholesterol-lowering effects of modified animal fats in postmenopausal women. University of Minnesota, Department of Food Science and Nutrition, St. Paul 55108-6099, USA. J Am Coll Nutr. 1997; 16(6):570-7.
Akanji M. A., Olagoke O. A. and Oloyede O. B. Effect of chronic consumption of metabisulphite on the integrity of the kidney cellular system. Toxicol 1993; 81:173-179.
Dufour D.R., Lott J. A., Nottle F. S. and Seff L.B. Diagnosis and monitoring of hepatic injury in performance characteristics of laboratory tests. Clin. Chem. 2000; 46: 2027-2049.
Shahjahan M., Sabitha K. E., Mallika J. and Shyamala-Devi C. S. Effect of Solanumtrilobatum against carbon tetrachloride induced hepatic damage in albino rats. Indian J. Med. Res. 2004; 120: 194-198.
Zikic R.V., Stajn A.S., Paulovic S.Z., Ogajanovic B.J. and Saicic Z.S. Activity of superoxide dismutase and catalase in Erythrocytes and plasma aminotransferases of goldfish exposed to Cadmium. Physio. Rev. 2001; 50:105-111.
Paula H., Pedrosa L. M., Júnior V. J. R., Haraguchi F. K., Santos R. C. and Silva M. E. Effect of an Aqueous Extract of Annatto (Bixa orellana) Seeds on Lipid Profile and Biochemical Markers of Renal and Hepatic Function in Hypercholesterolemic Rats. Braz. Arch. Biol. Technol. 2009; 52(6): pp. 1373-1378.
He G. and Aoyama Y. ().Effects of Adding Some Dietary Fibers to a Cystine Diet on the Activities of Liver Antioxidant Enzymes and Serum Enzymes in Rats. Biosci Biotechnol Biochem 2003; 67:617-621.
Martin P. and Friedman L.S. ().Assessment of Liver Function and Diagnostic Studies. In: Hand Book of Liver Disease. Friedmann, L .S. and E.B. Keeffe (Eds.), Churchill Livingstone, Philadelphia, 1-14. 1992.
Isaacs C. E., Litov R, Marie P. Thomas H. Addition of lipids to infant formula produces antiviral and antibacterial activity. J. Nutr. Biochem. 1992; 3: 304-308.
Potter S. M., Jimenez-Flores R., Pollack J., Lone T. A. and Berber-Jimenez M.D. Protein saponin interaction and its influence on blood lipids. J Agri Food Chem. 1993; 41: 1287-1291.
George F., Zohar K., Harinder P. S. and Klaus B. The biological action of saponins in animals systems: a review. Brit J Nutr. 2002; 88: 587-605.
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