Phytochemical Screening, Tyrosinase Inhibitory Effects and Kinetics of Cam Wood Dye Extracts
Advances in Biochemistry
Volume 4, Issue 2, April 2016, Pages: 16-20
Received: Feb. 5, 2016;
Accepted: Feb. 18, 2016;
Published: Apr. 28, 2016
Views 4779 Downloads 179
David Morakinyo Sanni, Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria
Oluwasegun Victor Omotoyinbo, Department of Biochemistry, Federal University of Technology, Akure, Ondo State, Nigeria
Tyrosinase is a key enzyme in melanin biosynthesis, as it catalyzes the oxidation of o-diphenols to o-quinones. Cam wood dye was extracted using methanol, acetone and dichloromethane; while extracts obtained were screened for Phytochemicals as well as their tyrosinase inhibitory effects and kinetic studies. Phytochemical screening of the three extracts showed the presence of total phlobatanins in all extracts except methanolic which had minute presence. Cardiac glycosides and flavonoids were also observed in the dichloromethane extracts and methanolic extract respectively. The methanolic extracts had best enzyme inhibition (84.1%) at highest concentration considered of 400µg/ml as compared to the dichloromethane and acetone extracts with peak values of 58.5 and 51.5% respectively. The enzyme kinetics analysis of substrate showed same inhibition type for three extracts which was non-competitive and its mechanism irreversible. The Michealis-Mentens constants for the three extracts were determined to be 0.344, 0.355 and 0.214mM, for acetone, dichloromethane and methanolic extracts respectively while the values of Vmax/Km shows inhibiting extracts followed the order: methanol extract > acetone extract > dichloromethame extract. The result therefore showed that methanolic extracts of Cam wood dye was the most effective in tyrosinase inhibition.
David Morakinyo Sanni,
Oluwasegun Victor Omotoyinbo,
Phytochemical Screening, Tyrosinase Inhibitory Effects and Kinetics of Cam Wood Dye Extracts, Advances in Biochemistry.
Vol. 4, No. 2,
2016, pp. 16-20.
Lichterman B. L. (2004). "Aspirin: The Story of a Wonder Drug". British Medical Journal, 329 (74-79): 1408.
Tapsell L. C., Hemphill I., Cobiac L., Patch C. S., Sullivan D. R., Fenech M., Roodenrys S., Keogh J. B., Clifton P. M., Williams P. G., Fazio V. A., Inge K. E. (2006). “Health benefits of herbs and spices: the past the present, the future.” Med. J. Aust. 185(4 Supply): S4-24.
Zawistowski J., Biliaderis C. G., Eskin N. A. M. (1991). Polyphenol oxidase. In: Oxidative Enzymes in Foods, Substrate Elsevier, London, 217-273.
Whitaker J. R. (1995). Polyphenol oxidase. In: Food Enzymes, Structure and Mechanism. Chapman and Hall, New York, 271-307.
Barton D. E., Kwon B. S., Francke U. (1988). "Human tyrosinase gene, mapped to chromosome, defines second region of homology with mouse chromosome 7".Genomics 3 (1): 17–24.
Kumar C. M., Sathisha U. V., Dharmesh S., Rao A.G., Singh S.A. (2011). "Interaction of sesamol (3, 4-methylenedioxyphenol) with tyrosinase and its effect on melanin synthesis". Biochimie 93 (3): 562–9.
Seiberg M., Paine C., Sharlow E., Andrade-Gordon P., Costanzo M., Eisinger M., Shapiro S.S. (2000). Inhibition of Melanosome Transfer Results in Skin Lightening. J. Investigative Dermatology 115, 162-167.
Petit L., Piérard G. E. (2003). Skin-lightening products revisited. International Journal of Cosmetic Science, 25: 169–181.
Brain K. R., Turner T. D. (1975). The practical evaluation of phytopharmaceuticals, Wright-science technical, Bristol Britain, 56-64.
Sofowora A. O. (1993). Medicinal Plants and Traditional Medicine in Africa. University of Ife Press 2nd Edition, 320.
Edeoga H. O., Okwu D. E., Mbaebie B. O. (2005). Phytochemical constituents of some Nigerian medical plants. African J. Biotechnology, 4: 685-688.
Trease G. E., Evans W. C. (2000). Pharmacognosy.14th Edition Harcourt Publishers Limited London.
Harbone N. V. (1994). Phytochemical methods: A guide to modern techniques of plant analysis, 2nd Edition, Chapman and Hall London., 425.
Osuagwu G. G. E., Okwulehie I. C., Emenike J. O. (2007). Phytochemical and Mineral content of the leaves of four Nigerian Pterocarpus species. Int. J. Mol. Med. Adv. Sci, 3(1): 6–11.
Ushie O. A., Adamu H. M. (2010). Phytochemical Screening of Borreria verticillata leaves. Journal of Agriculture, Biotechnology and Ecology, 3(1): 108-117.
Chun O. K., Chun S. J., Song W. O. (2007). Estimated dietary flavonoid intake and major food sources of US adults. J Nutr; 137:1244-52.
Momtaz S. (2006). Anti-tyrosinase activity of eleven plants. Honours Thesis; Diseases: What’s new? European Academy of Dermatology and Venereology JEADV, 17: 663-669.
Baek S., Kim J., Kim D., Lee C., Kim J., Chung D. K., Lee C. (2008). Inhibitory effect of dalbergioidin isolated from the trunk of Lespedeza cyrtobotrya on melanin biosynthesis. J. Microbiol. Biotechnol, 18, 874-879.
Huang Q., Zhu Y., Li H., Zhuang J., Zhang C., Zhou J., Li W., Chen Q. (2009). Inhibitory Effects of Methyl trans-Cinnamate on Mushroom Tyrosinase and its Antimicrobial Activities. J. Agric. Food Chem., DOI: 10.1021/jf8036227.
Xie J. J., Song K. K., Qui L., He Q., Huang H., Chen Q. X. (2007). Inhibitory effect of substrate and analogues on enzyme activity and substrate specificities of mushroom tyrosinase. Food Chem., 103, 1075-1079.