Advances in Biochemistry

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Antihyperglycemic Activity and Phytochemical Screening of Khaya Senegalensis (Meliaceae) in Wistar Rats

Received: 19 August 2016    Accepted: 09 September 2016    Published: 14 October 2016
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Abstract

The aim of this study was to identify the chemical group, evaluate an acute toxicity study and analyze the effect of aqueous extract Khaya senegalensis (Meliaceae), on experimentally induced hyperglycemic condition in rats. The phytochemical study of the aqueous extract of Khaya senegalensis revealed the presence of active secondary metabolic compounds such as flavonoids, polyphenols, catechol tannins, quinones, saponins, sterols and terpenes. The stem bark of Khaya senegalensis was used for acute toxicity study by gavage in Swiss mice. The aqueous extract of Khaya senegalensis (AEKS) at the dose of 35±5 mg/kg administrated by gavage showed that all the mice treated were living after 24 hours. The lethal dose obtained by intraperitoneal administration with graded doses to the mice was 1778 mg/kg. This extract could be non-toxic. The study of AEKS activity on blood glucose in rats showed a significant (p <0.01) the concentration of glucose in the serum of the treated rats with the AEKS (96.2 ± 30 mg/dl) and Glibenclamide (92.4±10 mg/dL), compared to glucose concentrations in the serum of hyperglycemic rats (197.1 ± 50 mg/dl). Thus, the study showed hypoglycemic activity of aqueous extract of Khaya senegalensis. In conclusion, the administration of AEKS causes hypoglycemic activity in rats given oral glucose load. Hypoglycemia this is due to the presence of active chemical groups in the extract of this plant, such as flavonoids, catechin tannins, polyphenols, quinones, sterols and terpenes. These results confirm the therapeutic indication in traditional medicine Khaya senegalensis in the treatment of diabetic disease.

DOI 10.11648/j.ab.20160405.11
Published in Advances in Biochemistry (Volume 4, Issue 5, October 2016)
Page(s) 53-57
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This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Khaya senegalensis, Phytochemical Screening, Toxicity, Hypoglycemic, Rat

References
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[2] Amiot MJ, Riollet C, Landrier JF. Polyphénols et syndrome métabolique. Médecine des maladies métaboliques, 2009 ; 3,5: 476-482.
[3] Awah PK. Diabète et médecine traditionnelle en Afrique. Soins de santé, Diabetes Voice, 2006; 51, 3: 24-26
[4] Breneton J. Pharmacolgnosie. Phytochimie. Plante médicinales. Paris Lavoisier, 2002; 3: 346-358.
[5] Choudhary SK, Chabra G, Sharma D. Comprehensive evaluation of antihyperglycemic activity off ractionated Momordica charantia seed extractin alloxan-induced diabetic rats. Complem Alternat Med, 2012; 29: 36-50.
[6] Eltayeb GM. Phytochemical Study of the flavonoids of the roots of Khaya senegalensis. Secheresse. URI. hdl. handle.net/. 2007; 123456789/3269.
[7] Goodarzi MT, ZalF, Malakooti M, Safari MR, Sadeghian S. Inhibitory activity off l on the lens aldose reductase of healthy and diabetic rats. Acta Med. Iran. 2006; 44,1: 41-5.
[8] Grover JK, Yadav S, Vats V. Medicinal plants of India with antidiabetic potential. J Ethnopharmacol, 2002; 81: 81-100.
[9] Idu M, Erhabor JO, Oshomoh EO, Ovuakpor Uvo PO. Phytochemical composition and antimicrobial properties of the seeds of Khaya senegalensis (Desc.) A. Juss. J Advanced Botanic and Zoology.2014; V1I4. DOI:10.15297/JABZ. V1I4.03.
[10] Jourdain B. Probabilités et statistiques. Ellipses, 2013; 2 vol. 76-85.
[11] Kerharo J, Adam JC. La pharmacopée sénégalaise traditionnelle: plantes médicinales et tropicales. Paris, Ed Vigot et frère. 1974; 433-435.
[12] Landrault N, Poucheret P, Azay J, Krosniak M, GascF and al. Effect of a polyphenols-enriched Chardonnay white vine in diabetic rats. J. Agric. Food Chem.,2003,51 (1): 311–318.
[13] N’doua LAR, Aoussi S et al. Effets hypoglycemique et anti-hyperglycemique de l’extrait ethanolique à 70% de racines de Rauvolfia vomitoria afzel (apocynaceae). Eur Sci J, 2015; 11, 657–711.
[14] Oghenesuvwe EE, Nwoke EE and Lotanna AD. 2014. Guidelines on dosage calculation and stock solution preparation in experimental animals’ studies. 2014; 18,4: 100- 106.
[15] Oyedemi S, Bradley G, Afolayan A. Antidiabetic activities of aqueous stem bark extract of Strychno shenningsii in streptozotocin nicotinamide type 2 diabetic rats. J of Pharmacological research. 2012; 11, 1: 221-238.
[16] Pousset JL. Plantes médicinales d’Afrique, comment les reconnaître et les utiliser? Edi-Sud, 2004; 95-98pp.
[17] Rheney CC, Kirk KK. Performance of three blood glucose meters. Ann pharmacother, 2000; 34 (3) 317-21.
[18] Yangni AA. Valorisation de la médicine traditionnelle africaine en Côte d’Ivoire. Edit: CEDA. 2054; 162-186.
Author Information
  • Medical Biochemistry Laboratory, University Teaching Hospital of Yopougon, Abidjan, C?te d’Ivoire

  • Medical Biochemistry Laboratory, University Teaching Hospital of Yopougon, Abidjan, C?te d’Ivoire

  • Medical Biochemistry Laboratory, Medical Sciences of University Felix Houphouēt-Boigny, Abidjan, C?te d’Ivoire

  • Medical and Basic Biochemistry Laboratory of Pasteur Institute, Abidjan, C?te d’Ivoire

  • Pasteur Institute, Abidjan, C?te d’Ivoire

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    Adeoti Mansour Franck, Gogahy Konan, Monteomo Gnate François, Camara-Cisse Massara, Djaman Allico Joseph, et al. (2016). Antihyperglycemic Activity and Phytochemical Screening of Khaya Senegalensis (Meliaceae) in Wistar Rats. Advances in Biochemistry, 4(5), 53-57. https://doi.org/10.11648/j.ab.20160405.11

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    Adeoti Mansour Franck; Gogahy Konan; Monteomo Gnate François; Camara-Cisse Massara; Djaman Allico Joseph, et al. Antihyperglycemic Activity and Phytochemical Screening of Khaya Senegalensis (Meliaceae) in Wistar Rats. Adv. Biochem. 2016, 4(5), 53-57. doi: 10.11648/j.ab.20160405.11

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    AMA Style

    Adeoti Mansour Franck, Gogahy Konan, Monteomo Gnate François, Camara-Cisse Massara, Djaman Allico Joseph, et al. Antihyperglycemic Activity and Phytochemical Screening of Khaya Senegalensis (Meliaceae) in Wistar Rats. Adv Biochem. 2016;4(5):53-57. doi: 10.11648/j.ab.20160405.11

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  • @article{10.11648/j.ab.20160405.11,
      author = {Adeoti Mansour Franck and Gogahy Konan and Monteomo Gnate François and Camara-Cisse Massara and Djaman Allico Joseph and Dosso Mireille},
      title = {Antihyperglycemic Activity and Phytochemical Screening of Khaya Senegalensis (Meliaceae) in Wistar Rats},
      journal = {Advances in Biochemistry},
      volume = {4},
      number = {5},
      pages = {53-57},
      doi = {10.11648/j.ab.20160405.11},
      url = {https://doi.org/10.11648/j.ab.20160405.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ab.20160405.11},
      abstract = {The aim of this study was to identify the chemical group, evaluate an acute toxicity study and analyze the effect of aqueous extract Khaya senegalensis (Meliaceae), on experimentally induced hyperglycemic condition in rats. The phytochemical study of the aqueous extract of Khaya senegalensis revealed the presence of active secondary metabolic compounds such as flavonoids, polyphenols, catechol tannins, quinones, saponins, sterols and terpenes. The stem bark of Khaya senegalensis was used for acute toxicity study by gavage in Swiss mice. The aqueous extract of Khaya senegalensis (AEKS) at the dose of 35±5 mg/kg administrated by gavage showed that all the mice treated were living after 24 hours. The lethal dose obtained by intraperitoneal administration with graded doses to the mice was 1778 mg/kg. This extract could be non-toxic. The study of AEKS activity on blood glucose in rats showed a significant (p <0.01) the concentration of glucose in the serum of the treated rats with the AEKS (96.2 ± 30 mg/dl) and Glibenclamide (92.4±10 mg/dL), compared to glucose concentrations in the serum of hyperglycemic rats (197.1 ± 50 mg/dl). Thus, the study showed hypoglycemic activity of aqueous extract of Khaya senegalensis. In conclusion, the administration of AEKS causes hypoglycemic activity in rats given oral glucose load. Hypoglycemia this is due to the presence of active chemical groups in the extract of this plant, such as flavonoids, catechin tannins, polyphenols, quinones, sterols and terpenes. These results confirm the therapeutic indication in traditional medicine Khaya senegalensis in the treatment of diabetic disease.},
     year = {2016}
    }
    

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    AU  - Adeoti Mansour Franck
    AU  - Gogahy Konan
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    AU  - Dosso Mireille
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    JF  - Advances in Biochemistry
    JO  - Advances in Biochemistry
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    PB  - Science Publishing Group
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    UR  - https://doi.org/10.11648/j.ab.20160405.11
    AB  - The aim of this study was to identify the chemical group, evaluate an acute toxicity study and analyze the effect of aqueous extract Khaya senegalensis (Meliaceae), on experimentally induced hyperglycemic condition in rats. The phytochemical study of the aqueous extract of Khaya senegalensis revealed the presence of active secondary metabolic compounds such as flavonoids, polyphenols, catechol tannins, quinones, saponins, sterols and terpenes. The stem bark of Khaya senegalensis was used for acute toxicity study by gavage in Swiss mice. The aqueous extract of Khaya senegalensis (AEKS) at the dose of 35±5 mg/kg administrated by gavage showed that all the mice treated were living after 24 hours. The lethal dose obtained by intraperitoneal administration with graded doses to the mice was 1778 mg/kg. This extract could be non-toxic. The study of AEKS activity on blood glucose in rats showed a significant (p <0.01) the concentration of glucose in the serum of the treated rats with the AEKS (96.2 ± 30 mg/dl) and Glibenclamide (92.4±10 mg/dL), compared to glucose concentrations in the serum of hyperglycemic rats (197.1 ± 50 mg/dl). Thus, the study showed hypoglycemic activity of aqueous extract of Khaya senegalensis. In conclusion, the administration of AEKS causes hypoglycemic activity in rats given oral glucose load. Hypoglycemia this is due to the presence of active chemical groups in the extract of this plant, such as flavonoids, catechin tannins, polyphenols, quinones, sterols and terpenes. These results confirm the therapeutic indication in traditional medicine Khaya senegalensis in the treatment of diabetic disease.
    VL  - 4
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