Heat Shock Protein 90 Alpha Genes Are Up-regulated by Acute Exercise
Advances in Biochemistry
Volume 5, Issue 4, August 2017, Pages: 57-60
Received: Mar. 22, 2017; Accepted: Apr. 15, 2017; Published: Jul. 5, 2017
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Ehiaghe Friday Alfred, Department of Hematology and Immunohematology, College of Health Sciences, Igbinedion University, Okada, Nigeria; Department of Medical Laboratory Science, College of Health Sciences, Igbinedion University, Okada, Nigeria; Lahor Research and Medical Centre, Benin City, Nigeria; Department of Medical Laboratory Science, Nnamdi Azikiwe University, Awka, Nigeria
Digban Kester, Department of Medical Laboratory Science, College of Health Sciences, Igbinedion University, Okada, Nigeria
Ehiaghe Imuetiyan Joy, Lahor Research and Medical Centre, Benin City, Nigeria
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This study was designed to further determine the expression pattern and role of heat shock protein 90 alpha genes in pre and post acute exercise amongst young male undergraduates in Nnewi, Nigeria. Twenty five (25) healthy young male undergraduate students with an average age of 24.3± 3 years and body mass index of 22.7± 1.8(Kg/m2) participated fully in the study. The subjects took part in an endurance race using the Bruce treadmill protocol for sub-maximal exercise for a maximum of 21 minutes. Blood samples were collected from the participants before commencement of the study, at 1 hour, 4 hours and 24 hours post exercise. Heat shock protein 90 alpha genes expression patterns were detected using reverse transcriptase polymerase chain reaction method. The expression pattern of heat shock protein 90 alpha genes was up-regulated at 1 hours post exercise and sustained till 24 hours post exercise at 200bp in all the exercised subjects when compared with the pre-exercise stage. Post exercise stress activates the release of heat shock protein 90 alpha genes which are needed to restore cellular homeostasis.
Acute Exercise, Heat Shock Protein 90 Alpha, Stress, Up-regulation
To cite this article
Ehiaghe Friday Alfred, Digban Kester, Ehiaghe Imuetiyan Joy, Heat Shock Protein 90 Alpha Genes Are Up-regulated by Acute Exercise, Advances in Biochemistry. Vol. 5, No. 4, 2017, pp. 57-60. doi: 10.11648/j.ab.20170504.11
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Stampfer, MJ., Hu, FB., Manson, JE., Rimm, EB., Willet, WC. Primary prevention of Coronary Heart Disease in Women through Diet and Lifestyle. New England Journal of Medicine.2000.343(1): 16-22.
Hu, FB., Manson, J., Stampfer, M., Graham, C.Diet, Lifestyle and risk of type 2 diabetes mellitus in women. The New England journal of Medicine.2001.345 (11): 790 – 797.
Gosker, HR., Scholes, AM. Fatigued muscles in COPD but no finishing line in sight. European Respiratory Journal. 2008.31:693-694.
Koolhaas, J. Stress revisited: A critical evaluation of the stress concept. Neuroscience andBehavioralReviews. 2011.35:1291–1301.
Linquist, S. The heat-shock response. Annual Review of Biochemistry. 1986.55: 1151-1191.
Abdalla, A O., Kiaii, S., Hansson, L., Rossmann, E.D., Jeddi-Tehrani, M., Shokri, F., Osterborg, A., Mellstedt, H., Rabbani, H. Kinetics of cytokine gene expression in human CD4 + and CD8+ lymphocyte subsets using quantitative real-time PCR. Scandinavian Journal of Immunology. 2003.58: 601- 606.
Katia, C., Michela, F., Stefano, C., Giacomo, S., Maurizio, S.A.V. Exercise induced stress in horses: Selection of the most stable reference genes for quantitative RT-PCR normalization.BMC Molecular Biology. 2008.9: 49-51.
Bermudez, DM., Canning, DA., Liechty, KN. Age and pro-inflammatory cytokine production: Wound-healing implications for scar-formation and the timing of genital surgery in boys. Journal of Pediatric Urology. 2011.7: 324- 331.
Josef, F. Biomarkers of peripheral muscle fatigue during exercise. Musculoskeletal Disorders. 2012.13: 213- 218.
Lars, MN, Ric, L, Leigh, M. Heat shock proteins in exercise: A review. Journal of Exercise Science and Physiotherapy. 2006.2: 13-26.
Vanessa, N. and Elizabeth, D. Submaximal exercise testing: Clinical application and interpretation. Journal of the American Physical Therapy Association.2000.80: 782- 807.
Ehiaghe F A, Onyenekwe C C, Akosile C O, Ehiaghe IJ, Oyeyemi A W, Ifeanyichukwu M O. Some lymphocytic gene expression patterns associated with acute exercise in young male undergraduates. American Journal of Biotechnology and Molecular Science. 2016, 5: 8-22.
De-Quevain, DJ., Roozendaal, B., Nitsch, RM., McGaugh, JL., Hock, C. Acute cortisone administration impairs retrival of long-term declaration memory in humans. Nature Neuroscience. 2005.4: 313- 314.
Febbrio, MA, Koukoulas, I. HSP72 gene expression progressively increases in human skeletal muscles during prolonged exhaustive exercise. Journal of Applied Physiology. 2000. 89: 1055-1060.
Fehrenbach, E., Passek, F., Niess, AM., Pohla, H., Weinstock, C., Dickhuth, H., Northoff, H. HSP expression in human leukocytes is modulated by endurance exercise. Medical Science. Sports and Exercise. 2000.32: 592-600.
Vamshi, KRG. Biological scavengers of free radical-antioxidant: International Journal of Pharmaceutical Biology and Chemical Science.2012.1: 13-19.
Giaccia, AJ., Auger, EA, Koong, A., Terris, DJ, Minchinton, AI, Hahn, GM, Brown, JM. Activation of heat shock transcription factors by hypoxia in normal and tumor cell lines in vivo and I vitro. International Journal of Radiation Oncology. Biol. Phys.1992. 23: 891-897.
Locke, M., Noble, EG. Stress proteins: The exercise response. Canadian Journal Applied Physiology. 1995.20: 155-167.
Kim, D., Ouyany, H., Li, GC. Heat shock protein HSP70 accelerates the recovery of heat shocked mammalian cells through its modulation of heat shock transcription factor HSFI. Proceedings of the National Academy Sciences.1995. 92: 2126-2130.
Ehiaghe FA, Onyenekwe CC, Akosile CO, Okoye C, Maruf FA, Agbonlahor DE, Uwabor CI, Ehiaghe IJ, Ogbu EC. The expression pattern and role of interferon gamma genes in exhaustive exercise. Journal of Advance in Biology. 2016. 9: 1903-1910.
Ehiaghe FA, Onyenekwe CC, Ehiaghe IJ, Akosile CO, Agbonlahor DE. The role of cortisol and interleukin-10 gene expression patterns in exhaustive exercise. International Journal of Biological and Chemical Sciences. Accepted.
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