Please enter verification code
Production of Bio-Hydrogen Gas and Other Metabolic Gases by Anaerobic Bacteria Grown on Molasses
Advances in Biochemistry
Volume 5, Issue 6, December 2017, Pages: 110-116
Received: Sep. 28, 2017; Accepted: Oct. 12, 2017; Published: Nov. 22, 2017
Views 3677      Downloads 335
Rasha Jame, Department of Chemistry, Faculty of Science, University of Tabuk, Tabuk, Saudi Arabia; Department of Chemistry, Faculty of Education, University of Dalanj, Dalanj, Sudan; Department of Chemistry, Faculty of Science, University of Slovak, Bratislava, Slovak Republic
Boris Lakatoš, Department of Chemistry, Faculty of Science, University of Slovak, Bratislava, Slovak Republic
Mawia Hassan, Department of Chemistry, Faculty of Science and Technology, University of Abdulatif, Alhamad- Merwe, Sudan
Mutasim Elhag, Department of Chemistry, Faculty of Education, University of Nile Valley, Atbra, Sudan
Ludovit Varečka, Department of Chemistry, Faculty of Science, University of Slovak, Bratislava, Slovak Republic
Article Tools
Follow on us
The study had aimed to characterize the production of hydrogen gases by anaerobic bacteria. One isolate was found in sheep ruminal fluid and four isolates were obtained from the activated sludge. These isolates were identified by microscopic methods and by rRNA sequences. One ruminal bacterium was identified as Escherichia coli, and it was found that these isolates from activated sludge were related to Clostridium botulinum, C. perfringens and C. difficile. One strain could not be assigned to any species but was similar to C. botulinum. Growth and production of the metabolic gases with molasses as sole carbon source were measured during the anaerobic cultivation by Micro-Oxymax (Columbus Instruments, Columbus, OH, U.S.A.) gas analyzer. One of the most available saccharidic waste products is molasses. The growth on molasses as carbon source was done to test the production of H2. It was found that all tested Clostridium isolates (AK 1-4, AK 1-5, AK 1-9 and AK 1-12) and E. coli isolate (No 2- 24) had utilized molasses as carbon source monitored by production of CO2 gas. All these strains produced H2 gas, and CO gas in concentration range 102 μmol L–1, and H2S gas in concentrations lower by one order of magnitude. Kinetics of evolution of these gases was different suggesting that they are produced by independent processes. Results show that metabolic gases are produced mainly in the exponential phase of growth.
Clostridium Spp., E. Coli, Molasses, Hydrogen, Metabolic Gases, Anaerobic Metabolism
To cite this article
Rasha Jame, Boris Lakatoš, Mawia Hassan, Mutasim Elhag, Ludovit Varečka, Production of Bio-Hydrogen Gas and Other Metabolic Gases by Anaerobic Bacteria Grown on Molasses, Advances in Biochemistry. Vol. 5, No. 6, 2017, pp. 110-116. doi: 10.11648/j.ab.20170506.11
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Levin DB, Pitt L, Love M., (2004), Biohydrogen production: prospects and limitations to practical application. Int J Hydrogen Energy, 29, 173–85.
Hawkes FR, Hussy I, Kyazze G, Dinsdale R, Hawkes DL. Continuous dark fermentative hydrogen production by mesophilic mivroflora: principles and progress. Int J Hydrogen Energy, 32, 172–184, 2007.
Thong S, Prasertsan P, Birkeland NK. (2009), Evaluation of methods for preparing hydrogen-producing seed inocula under thermophilic condition by process performance and microbial community analysis. Bioresour Technol, 100: 909-918.
Chang JS, Lee KS, Lin PJ. 2002, Biohydrogen production with fixed-bed bioreactors. Int J Hydrogen Energy, 27, 1167–74.
Shi XY, Jin DW, Sun QY, Li WW., 2010, Optimization of conditions for hydrogen production from brewery wastewater by anaerobic sludge using desirability function approach. Renew Energ, 35, 1493-1498.
Nan-Qi Ren, Dong-Yang Wang, Chuan-Ping Yang, Lu Wang, Jing-Li Xu, Yong-Feng Li., 2010, Selection and isolation of hydrogen-producing fermentative bacteria with high yield and rate and its bioaugmentation process. Int J Hydrogen Energy, 35, 2877-2882.
Hallenbeck PC, Benemann JR (2002) Int. J. Hydrogen Energy 27: 1185–1193.
Nath K, Das D (2004) Improvement of fermentative hydrogen production: various approaches. Appl. Microbiol. Biotechnol. 65: 520–529.
Hallenbeck PC, Ghosh D (2009) Trends in Biotechnology 27: 287-297.
Rasha Jame, Viera Vilímová, Boris Lakatoš, Ľudovít Varečka (2011). The hydrogen production by anaerobic bacteria grown on glucose and glycerol, Acta Chimica Slovaca, 4: 145–157.
Rasha Jame, V. Zelená, Boris Lakatoš, Ľudovít Varečka (2016). Carbon source utilization and hydrogen production by isolated anaerobic bacteria, Acta Chimica Slovaca, 9: 62-67.
Calusinska M, Happe T, Joris B, Wilmotte A (2010) Microbiology 156: 1575-1588.
Lin P-Y, Whang L-M, Wu Y-R, Ren W-J, Hsiao C-J, Li S-L, Chang J-S (2007) Int. J. Hydrogen Energy 32 1728-1735.
Guo XM, Trably E, Latrille E, Carrere H, Steyer J-P (2010) Int. J. hydrogen energy 35: 10660-10673.
Hiligsmann S, Masset J, Hamilton C, Beckers L, Philippe Thonart P (2011) Bioresource Technol. 102: 3810–3818.
Doi T., Matsumoto H, Abe J, Morita S (2010) Int. J. Hydrogen Energy 35: 7369-7376.
Pattra S, Sangyoka S, Boonmee M, Reungsanga A (2008) Int. J. Hydrogen Energy 33: 5256–5265.
Nissila ME, Tähti HP, Rintala JA, Puhakka JA (2011) Int. J. Hydrogen Production (2011) 36: 1482-1490.
Geng A, He Y, Qian C, Yan X, Zhou Z (2010) Bioresource Technol. 101: 4029–4033.
Sunil S. Adav, Duu-Jong Lee, Aijie Wang, Nanqi Ren Bioresource Technology 100 (2009) 2546–2550.
Fang HHP, Zhu H, Zhang T (2006) Int. J. Hydrogen Energy 31: 2223–2230.
Beckers L, Hiligsmann S, Hamilton C, Masset J, Thonart P (2010) Biotechnol. Agron. Soc. Environ. 14: 541-548.
Subudhi S, Lal B (2011) Int. J. Hydrogen Energy, doi:10.1016/j.ij hydene.2011.04.027.
Klein M, Marion B. Ansorge-Schumacher MB, Fritsch M, Hartmeier W (2010) Enzyme Microb. Technol. 46: 384–390.
Han Wei, Wang Bing, Liu Xiaoye, Liu Chunyu, Yue Liran, Li Yongfeng (2012), Characteristics of H2-producing fermentation with the immobilized mixed consortium were investigated using molasses as the sole carbon substrate, IJEE ], 2: 28-31.
Wan-Qian Guo, Nan-Qi Ren, Xiang-Jing Wang, Wen-Sheng Xiang, Zhao-Hui Meng, Jie Ding, Yuan-Yuan Qu, Lu-Si Zhang (2008), Biohydrogen production from ethanol-type fermentation of molasses in an expanded granular sludge bed (EGSB) reactor, International Journal of Hydrogen Energy, 33: 4981-4988.
Kalil MS, Alshiyab HSS, Yusoff WMW (2009) Am. J. Appl. Sci. 6: 1158-1168.
Piela P., Tokarz W., Kazmierczak W., Detman A., Sikora A., PiotrowskiI J. (2016), The use of hydrogen-rich gas obtained from dark fermentation of molasses from sugar industry for fueling a fuel cell. Przemysl Chemiczny 95 (5), 1000.
Anna Detman, Aleksandra Chojnacka, Mieczysław Błaszczyk, Wiktor Kaźmierczak, Jan Piotrowski, Anna Sikora (2017), Biohydrogen and biomethane (Biogas) production in the consecutive stages of anaerobic digestion of molasses, Pol. J. Environ. Stud. 26: 1023-1029.
Chojnacka A., Szczesny P., Blaszcz M. K., Zielenkiewicz U., Detman A., Salanon A., Sikora A. (2015), Noteworthy acts about a methane-producing microbial community processing acidic effluent from sugar beet molasses fermentation. PLoS One 10 (5), e0128008.
Science Publishing Group
1 Rockefeller Plaza,
10th and 11th Floors,
New York, NY 10020
Tel: (001)347-983-5186