Advances in Biochemistry

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Heterogeneous Phyto-Antibiotics and Other Future Therapeutics Against Multi-Drug Resistant Bacteria

Received: 27 June 2019    Accepted: 30 July 2019    Published: 23 August 2019
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Abstract

Phage therapy, enzybiotics, gene medicine and nanodrug-carriers are most vital components of modern research against multidrug-resistant bacteria. Genome evolutionoccurs due to environmental toxicities of various types where soil bacteria are in symbiosis with plant world who secret anti-metabolites against soil bacteria. Gut microbiome secrete vitamins required for our body where as intestinal cells synthesis interleukins and cytokines for symbiotic control of intestinal bacteria. Before the discovery of antibiotic in 1928, peoples trusted plant derived remedies against variety of illness described in Indian Sanskrit books like Charaka Samhita, Sasruta Samhita and Atharva Veda. AMR disease occurred due to repeated but uncontrolled use of antibiotics affecting gut microbiome who acquired many mdr genes in plasmids. We estimated that are 40% of all river and sea water borne bacteria were ampicillin and to lesser extent tetracycline, azithromycin, ciprofloxacin and streptomycin resistant. Where as <1% were multidrug-resistant and ~0.002% Enterobacteriaceae were meropenem, linezolid or amikacin resistant. MDR genes like amp, neo, tet, aac, cat, aph, aad, mcr-1, blaNDM-1, blaKPC-1, arr3, sul1, dhfr, inh, acrAB, mexAB, macAB and mtrCD were amplified with millions mutated isomers increasing all drugs MIC. We studied Indian medicinal plants and spices to get valuable cheap drugs where modified agriculture and/or tissue culture increased the drug concentration. We showed that MDR bacteria were sensitive to organic extract of Suregada multiflora roots, Cassia fistula bark, Jatropha gossypifolia roots as well as Indian spices Labanga and Derchini (MDR-Cure). TLC and HPLC purification as well as UV-VIS, MASS, NMR, FT-IR and XRD-Powder gave many distinct signatures of pure chemicals that also inhibited multidrug-resistant bacteria. Biological targets of 12 chemicals are under investigation targeting DNA Topoisomerase I, DNA Polymerase and RNA Polymerase of Escherichia coli. Indian Government has started Herbal Mission, Ganga Mission and various Plantation Programmes to curve MDR pathogenesis.

DOI 10.11648/j.ab.20190702.11
Published in Advances in Biochemistry (Volume 7, Issue 2, June 2019)
Page(s) 34-50
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Multidrug-Resistance, Superbug Spread, Antibiotic Void, Drug Design, Heterogeneous Phyto-Antibiotics, Gut Biofilm, Vitamin Synthesis

References
[1] Tortora, G. J., B. R. Funke, and C. L. Case. Microbiology: An Introduction. 12thedi. 2016. ISBN: 9780321929150.
[2] Sebstian P. Ayurvedic Medicine. The principles of traditional practice. 1st Edition 2006. Elsevier Press, ISBN: 9780443100901.
[3] Ackerknecht, E. H. A Short History of Medicine. Baltimore, MD. The Johns Hopkins University Press, 1982.
[4] Chakraborty, A. K (2019) Conceptual Drug Discovery and Societal Status may not be Sufficient to Combat Multidrug-Resistant Infections. Pharmaceutical Regulatory Affairs 7, 213. doi: 10. 4172/2167-7689. 1000213.
[5] Chakraborty, A. K., K. Poira., D. Saha., C. Halder., S. Das et al. (2018) Multidrug- Resistant Bacteria with activated and diversified MDR Genes in Kolkata Water: Ganga Action Plan and Heterogeneous Phyto-Antibiotics tackling superbug spread in India. American Journal of Drug Delivery and Therapeutics 5 (1), 1-9.
[6] Maniatis, T., E. F. Fritsch and Sambrook, J (1982) Molecular Cloning-A Laboratory Manual. Cold Spring Harbour Laboratory, 1982. ISBN: 978-0879691363.
[7] Carey, F. A. and R. A. Sundberg. Advanced Organic Chemistry, 1st edition, Springer, 2005. ISBN: 978-0-387-68350-8.
[8] Chakraborty, A. K (2017) Ganga action plan, heterogeneous phyto-antibiotics and phage therapy are the best hope for India tackling superbug spread and control. Indian Journal of Biological Sciences 23, 34-51.
[9] Sigerst, H. E. A History of Medicine: Early Greek, Hindu and Persian Medicine, vol. II, New York, Oxford University Press, 1987. ISBN: 9780195050790.
[10] Chakraborty, A. K (2019) Current status and unusual mechanism of multi-resistance in Mycobacterium tuberculosis. Journal of Health and Medical Informatics 10, 328. DOI: 10. 4172/2157-7420. 1000328.
[11] Chakraborty, A. K (2018) Worldwide spread of MDR bacteria signals to be resident of gut microbiota for vitamins synthesis and heterogeneous phyto-antibiotics may cure MDR infections globally. Biomedical Research 29, 50. doi: 10. 4066/biomedicalresearch-C1-003.
[12] Chakraborty, A. K (2017) Multi-drug resistant bacteria from Kolkata Ganga River with heterogeneous MDR genes have four hallmarks of cancer cells but could be controlled by organic phyto-extracts. Bioc hemistry and Biotechnology Research 5 (1), 11-23.
[13] Sylvia, D. M., P. G. Hartel., J. J. Fuhrmann and D. A. Zuberer. Principle and Application of Soil Microbiology. 2nd ed. Pearson Prentice Hall, 2005.
[14] Chakraborty, A. K (2015) High mode contamination of multi-drug resistant bacteria in Kolkata: mechanism of gene activation and remedy by heterogeneous phyto-antibiotics. Indian Journal of Biotechnology 14, 149-159.
[15] Chakraborty, A. K., G. E. Muneim., S. Pradhan and A. Adhikari (2018) Superbug horror and its relations to antibiotics, probiotics and vitamins. Journal of Pharmaceutical Toxicology1 (1), 8-13.
[16] Thakar, V. J (2010) Historical development of basic concepts of Ayurveda from Veda up to Samhita. Ayu3 (4). 400-402. doi: 10. 4103/0974-8520. 82024.
[17] Singh, R., S. Hussian., R. Vermaan and P. Sarma (2013) Anti-mycobacterial screening of five Indian medicinal plants and partial purification of active principle from Cassia sophera and Urtica dioica. Asian Pacific Journal of Tropical Medicine 6 (5), 366-371.
[18] Clayton, J. C., B. A. Hems., F. A. Robinson., R. D. Andrews and R. F. Hunwicke (1944) Preparation of penicillin. Improved method of isolation. Biochemical Journal 38 (5), 452-458.
[19] Abraham, E. P and E. Chain (1940) An enzyme from bacteria able to destroy penicillin. Rev Infectious Diseases 1988; 10, 677-678.
[20] Chakraborty, A. K (2017) MDR genes are created and transmitted in plasmids and chromosomes to keep normal intestinal microbiota alive against high dose antibiotics- A hypothesis. Journal of Molecular Medicine and Clinical Application 2 (1), 109. doi: 10. 16966/2575-0305. 109.
[21] Le Chatelier, E., T. Nielsen., J. Qin., E. Prifti., F. Hildebrand., et al. (2013) Richness of human gut microbiome correlates with metabolic markers. Nature 500, 541-546.
[22] Ram, J. L., A. S. Karim., E. D. Sendler and I. Kato (2011) Strategy for microbiome analysis using 16S gene sequence analysis on the Illumina sequencing platform. System Biology and Reproduction Medicine 57 (3), 162-170. doi: 10. 3109/19396368. 2011. 555598.
[23] Sanger, F., S. Nicklen and A. R. Coulson (1977) DNA sequencing with chainterminating inhibitors. Proceeding of the National Academy of Sciences USA 74, 5463-5467.
[24] Pérez-Cobas, A. E., A. Artacho., H. Knecht., M. L. Ferrús., A. Friedrichs, et al. (2013) Differential effects of antibiotic therapy on the structure and function of human gut microbiota. PLoS One. 8 (11). e80201. doi: 10. 1371/journal. pone. 0080201.
[25] Salyers, A. A., A. Gupta and Y. Wang (2004) Human intestinal bacteria as reservoirs for antibiotic resistance genes. Trends Microbiology 12 (9), 412-416.
[26] Wei, S., M. Morrison and Z. Yu (2013) Bacterial census of poultry intestinal microbiome. Poultry Science92 (3), 671-683. doi: 10. 3382/ps. 2012-02822.
[27] Chakraborty, A. K (2018) Superbugs spread and problems of multidrug-resistant infections during surgery. International Journal of Surgery and Invasive Procedures 1 (1), 6-13.
[28] Chakraborty, A. K (2016) Multi-drug resistant genes in bacteria and 21st Century problems associated with antibiotic therapy. Biotechnology: An Indian Journal 12 (12), 113.
[29] Wani, M. C., H. L. Taylor., M. E. Wall., P. Coggon and A. T. McPhail (1971) Plant antitumor agents. VI. The isolation and structure of taxol, a novel anti-leukemic and anti-tumor agent from Taxus brevifolia. Journal of American Chemical Society 93 (9), 2325-2327.
[30] Paddon, C. J., P. J. Westfall., D. J. Pitera., K. Benjamin., K. Fisher., et al. (2013) High-level semi-synthetic production of the potent antimalarial artemisinin. Nature 49,: 528-532.
[31] Heinig, U., S. Scholz and S. Jennewein (2013) Getting to the bottom of taxol biosynthesis by fungi. Fungal Diversity60, 161–70. doi: 10. 1007/s13225-013-0228-7.
[32] Stierle, A., G. Strobel and D. Stierle (1993) Taxol and taxane production by Taxomyces andreanae, an endophytic fungus of Pacific yew. Science 260 (5105), 214–216. doi: 10. 1126/science. 8097061.
[33] Hsiang, Y. H., R. Hertzberg., S. Hecht and L. F. Liu (1985) Camptothecin induces protein-linked DNA breaks via mammalian DNA topoisomerase I. Journal of Biological Chemistry 260, 14873-14878..
[34] Staines, H. M and S. Krishna. Treatment and prevention of malaria: Antimalarial drug chemistry, action and use. Springer Verlag. 2011; p. 45. ISBN: 9783034604796.
[35] Ro, D. K., E. M. Paradise., M. Ouellet., K. J. Fisher., K. L. Newman., et al. (2006) Production of the antimalarial drug precursor artemisinic acid in engineered yeast. Nature440 (7086), 940–943. doi: 10. 1038/nature04640.
[36] Woodward, Rand W. Doering (1944) The Total Synthesis of Quinine. Journal of American Chemical Soc iety66, 849.
[37] Lévesque, F and P. H. Seeberger (2012) Continuous-Flow synthesis of the anti-malaria drug artemisinin. Angewandte Chemie (International Edition) 51 (7), 1706–1709. doi: 10. 1002/anie. 201107446.
[38] Handa, S. S., S. P. S. Khanuja., G. Longo and D. D. Rakesh (2008) Extraction Technologies for medicinal and aromatic plants, (1st edn), no. 66. 2008. Italy: United Nations Industrial Development Organization and the International Centre for Science and High Technology.
[39] Guo, N., Y. W. Jiang., P. Kou., Z. M. Liu., T. Efferth., et al (2019) Application of integrative cloud point extraction and concentration for the analysis of polyphenols and alkaloids in mulberry leaves. Journal of Pharmaceutical and Biomedical Analysis. 167, 132-139. doi: 10. 1016/j. jpba. 2019. 02. 002.
[40] Vongsak, B., P. Sithisarn., S. S. Mangmool., S. Thongpraditchote., Y. Wongkrajang., et al. (2013) Maximizing total phenolics, total flavonoids contents and antioxidant activity of Moringaoleifera leaf extract by the appropriate extraction method. Industrial Crops and Products 44, 566-571.
[41] Dhooghe, L., K. Mesia., E. Kohtala., L. Tona., L. Pieters., et al. (2008) Development and validation of an HPLC-method for the determination of alkaloids in the stem bark extract of Nauclea pobeguinii. Talanta76 (2), 462-468. doi: 10. 1016/j. talanta. 2008. 03. 036.
[42] Kandiah, M and P. L. Urban (2013) Advances in ultrasensitive mass spectrometry of organic molecules. Chemical Society Reviews 42 (12), 5299–5322. doi: 10. 1039/c3cs35389c.
[43] Sobott, F. Biological Mass Spectrometry. Boc a Raton: CRC Press-I LLC, 2014. ISBN: 978-1439895276.
[44] Pavia, D. L., G. M. Lampman., G. S. Kriz and J. R. Introduction to Spectroscopy. 5thedi. Chapter 6, Pp. 290-347, 2013. Cengage Learning. ISBN: 978-1-285-46012-3.
[45] Dyer, J. R. Applications of absorption spectroscopy of organic compounds. Eastern Economy Edition, Chapter 3, Pp. 22-57, 2015. PHI Learning Private Limited. ISBN: 978-81-203-0252-5.
[46] Sastri, B. N. Ed. The Wealth of India, Raw Materials, Vol. IV, 1956, CSIR, New Delhi, India.
[47] Cowan, M. M (1999) Plant products as antimicrobial agents. Clinical Microbiology Review 12, 564–582.
[48] Laxminarayana, R (2014) Antibiotic effectiveness: Balancing conservation against innovation. Science 345, 1299-3101.
[49] Noumedem, J. A., M. Mihasan., S. T. Lacmata., M. Stefan., J. R. Kuiate., et al. (2013) Antibacterial activities of the methanol extracts of ten Cameroonian vegetables against Gram-negative multidrug-resistant bacteria. BMC Complementary and Alternative Medicine 13, 26-34.
[50] Daglia, M (2011) Polyphenols as antimicrobial agents. Current Opinion on Biotechnology23: 174-181.
[51] Chaieb, K., H. Hajlaoui, T. Zmantar., A. B. Kahla-Nakbi. M. Rouabhia., et al. (2007) The chemical composition and biological activity of clove essential oil, Eugenia caryophyllata (Syzygiumaromaticum L. Myrtaceae): a short review. Phytotherapy Research 21 (6), 501-506.
[52] Dubey, R. C., A. Rana and R. K. Shukla (2005) Antibacterial activity of essential oils of some medicinal plants against certain human pathogens. Indian Drugs 42, 443–446.
[53] Elumalai, S., R. Kesavan., S. Ramganesh., V. Prakasamand., R. Murugasen (2010) Comparative study on anti-microbial activities of bark oil extract from Cinnamomum cassia and Cinnamomum zeylanicum. Bioscience and Biotechnology Research Asia 7, 251–258.
[54] Lee, J. H., S. Cho., H. D. Paik., C. W. Choi., K. T. Nam., et al. (2014) Investigation on antibacterial and antioxidant activities, phenolic and flavonoid contents of some Thai edible plants as an alternative for antibiotics. Asian-Australasian Journal of Animal Sciences. 27 (10), 1461-1468.
[55] Chakraborty, A. K. Heterogeneous Phyto-Antibiotics will be Future Drugs against Superbugs. Proceeding of the 106th Indian Science Congress, Medicinal Sciences Section, Jan. 2-7, 2019. Lovely Professional University, India.
[56] Chakraborty, A. K., A. Khatoon., M. Maity., S. K. Nandi and U. Maity. Screening of some clinically important anti-bacterial phyto-extracts as described in Chiranjib Bonoushadhi targeting Kolkata superbugs. In: “Health, Nutrition and Hygiene: The Dynamics of Social Ecology in India” (edi. Sinha, Patsa, Das, Samanta), Chapter 30, pp. 260-278, 2016. ISBN: 978-81-930138-1-6.
[57] Kutter, E. M., S. J. Kuhland and S. T. Abedon (2015) Re-establishing a place for phage therapy in western medicine. Future Microbiology 10, 685–588.
[58] Jensen, K. C., B. B. Hair., T. M. Wienclaw., M. H. Murd k., J. B. Hatch., et al. (2015) Isolation and host range of bacteriophage with lytic activity against methicillin-resistant Staphyl cus aureus and potential use as a fomite decontaminant. PLoS One10 (7), e0131714.
[59] Dalmasso, M., R. Strain., H. Neve., C. M. Franz., F. J. Cousin., et al. (2016) Three new Escherichia coli phages from the human gut show promising potential for Phage Therapy. PLoS One 11 (6), e0156773. doi: 10. 1371/journal. pone. 0156773.
[60] Yu, Y. -P., T. Gong., G. Jost., W. -H. Liu., D. -Z. Ye and Z. -H. Luo (2013) Isolation and characterization of five lytic bacteriophages infecting a Vibrio strain closely related to Vibrio owensii. FEMS Microbioogyl Letters348 (2), 112–119.
[61] Yang, H., L. Liang., S. Lin andS. Jia (2010) Isolation and characterization of a virulent bacteriophage AB1 of Acinetobacterbaumannii. BMC Microbiology10 (1), 131. doi: 10. 1186/1471-2180-10-131.
[62] Carey-Smith, G. V., C. Billington., A. J. Cornelius., J. A. Hudson andJ. A. Heinemann (2006) Isolation and characterization of bacteriophages infecting Salmonella spp. FEMS Microbiology Letters258 (2), 182–186.
[63] Schooley, R. T., B. Biswas., J. J. Gill., A. Hernandez-Morales., J. Lancaster., L. Lessor., et al. (2017) Development and use of personalized bacteriophage-based therapeutic coc ktails to treat a patient with a disseminated resistant Acinetobacterbaumannii infection. Antimicrobial Agents and Chemotherapy 61 (10). pii: e00954-17. doi: 10. 1128/AAC. 00954-17.
[64] Chakraborty, A. K (2017) Enzybiotics, A new class of enzyme antimicrobials targeted against multidrug-resistant superbugs. Novel Approach of Drug Design and Development 2 (4), 555592.
[65] Chakraborty, A. K (2017) Diversified mdr genes in bacterial plasmids and chromosomes inactivate hundred drugs with huge superbug spread in sea, river and rain water. Journal of Pharmacovigilance 5 (Suppl 4), 29. doi: 10. 417/2329-6887-C1-029.
[66] Rodríguez-Cerrato, V., P. García., L. Huelves., E. García., G. Del Prado., M. Gracia., et al. (2007) Pneumococcal LytA autolysin, a potent therapeutic agent in peritonitis-sepsis caused by highly beta-lactamase resistant Streptococcus pneumoniae. Antimicrobial Agents and Chemotherapy 51 (9), 3371-3373.
[67] Yang, H., Y. Zhang., J. Yu., Y. Huang., X. E. Zhang., et al. (2014) A novel chimeric lysine with high antimicrobial activity against methicillin-resistant Staphylococ cus aureus in vitro and in vivo. Antimicrobial Agents Chemotherapy 58 (1), 536-542.
[68] Fernandes, S., D. Proença., C. Cantante., F. A. Silva., C. Leandro., et al. (2012) Novel chimerical endolysins with broad antimicrobial activity against methicillin-resistant Staphylococcus aureus. Microbial drug resistance 18, 333–343. doi: 10. 1089/mdr. 2012. 0025.
[69] Wu, H., J. Huang., H. Lu., G. Li and Q. Huang (2014) GMEnzy: A genetically modified enzymatic database. PLoS One 9 (8), e103687.
[70] Rodríguez-Rubio, L., D. Gutiérrez., D. M. Donovan., B. Martínez., A. Rodríguez., et al. (2016) Phage lytic proteins: biotechnological applications beyond clinical antimicrobials. Critical Review in Biotechnology 36 (3), 542-552. doi: 10. 3109/07388551. 2014. 993587.
[71] Borysowaski, J and A. Gorski. Enzybiotics and their potential application in medicine. In: Villa TG, Veiga-Crespo P., editors. Enzybiotics: antibiotic enzymes as drugs and therapeutics, First edi. Pp. 199-218, 2009. New Jersy: John Wiley & Sons Inc. Publication. ISBN: 9780470376553.
[72] Hojckova, K., M. Stano and L. Klucar (2013) phiBIOTICS: catalogue of therapeutic enzybiotics, relevant research studies and practical applications. BMC Microbiology 13, 53. doi: 10.1186/1471-2180-13-53.
[73] Zhao, X., H. Wu., H. Lu., G. Li and Q. Huang (2013) LAMP: A Database Linking Antimicrobial Peptides. PLoS One; 8 (6), e66557. doi: 10. 1371/journal. pone. 0066557.
[74] Rashel, M., J. Uchiyama., T. Ujihara., Y. Uehara., S. Kuramoto., et al. (2007) Efficent elimination of multidrug-resistant S. aureus by cloned Lysin derived from bacteriophage Phi MR11. Journal of Infecious Diseases19 (8), 1237-1247.
[75] Becker, S. C., F. J. Foster andD. M. Donovan (2008) ThephageK lytic enzyme LysK and lysostaphin act synergistically to kill MRSA. FEMS Microbiology Letters 287 (2), 185-191.
[76] Baroni, D and P. Arrigo (2014) MicroRNA target and gene validation in viruses and bacteria. Methods in Molecular Biology 1107, 223–231. doi: 10. 1007/978-1-62703-748-8_13.
[77] Ren, N., G. Gao., Y. Sun., L. Zhang., H. Wang., et al. (2015) MicroRNA signatures from multidrug resistant Mycobacterium tuberculosis. Molecular Medicine Reports 12 (5), 6561-6567. doi: 10. 3892/mmr. 2015. 4262.
[78] Cui, J. Y., H. W. Liang., X. L. Pan., D. Li., N. Jiao., et al. (2017) Characterization of a novel panel of plasma microRNAs that discriminates between Mycobacterium tuberculosis infection and healthy individuals. PLoS One12 (9), e0184113. doi: 10. 1371/journal. pone. 0184113.
[79] Meng, J., F. Da., X. Ma., N. Wang., Y. Wang., et al. (2015) Antisense growth inhibition of methicillin-resistant Staphylococcus aureus by loc ked nucleic acid conjugated with cell penetrating peptide as a novel FtsZ inhibitor. Antimicrobial Agents Chemotherapy59 (2), 914-922.
[80] Das, A. R., N. Dattagupta., C. N. Sridhar., W. K. Wu (2003) A novel thiocationic liposomal formulation of antisense oligonucleotides with activity against Mycobacterium tuberculosis. Scandinavian Journal of Infectious Diseases 35 (3), 168-174.
[81] Yu, K. R., H. Natanson and C. E. Dunbar (2016) Gene Editing of human hematopoietic stem and progenitor cells: promise and potential hurdles. Human Gene Therapy 27 (10), 729-740. doi: 10. 1089/hum. 2016. 107.
[82] Chakraborty, A. K., M. A. Zink and C. P. Hodgson (1995) Expression of VL30 vectors in human cells that are targets for gene therapy. Biohemical Biophysical Research Communication 209 (2), 677-683.
[83] Baker, C., A. Pradhan., L. Pakstis., D. J. Pochan and S. I. Shah (2005) Synthesis and antibacterial properties of silver nanoparticles. Journal of Nanoscience and Nanotechnology5 (2), 244-249.
[84] Chakraborty, A. K. Nucleic-Acids Based Nanocarriers, in “Nanocarriers for Drug Delivery”. eds. Mahapatra et al. 2018; Chapter-5; Pp. 155-172, Elsevier Press, Amsterdam. ISBN: 978012814.
[85] Chakraborty, A. K., S. Pradhan., S. Das., M. Maity., S. Sahoo., et al. (2019) Complexity of OXA beta-lactamases involved in multi-resistance. British Journal of Bio-Medical Research 3 (1): 772-798.
[86] Pelgrift, R. Y and A. J. Friedman (2013) Nanotechnology as a therapeutic tool to combat microbial resistance. Advanced Drug Delivery Review 65 (13), 1803-1815.
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    Asit Kumar Chakraborty. (2019). Heterogeneous Phyto-Antibiotics and Other Future Therapeutics Against Multi-Drug Resistant Bacteria. Advances in Biochemistry, 7(2), 34-50. https://doi.org/10.11648/j.ab.20190702.11

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    Asit Kumar Chakraborty. Heterogeneous Phyto-Antibiotics and Other Future Therapeutics Against Multi-Drug Resistant Bacteria. Adv. Biochem. 2019, 7(2), 34-50. doi: 10.11648/j.ab.20190702.11

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    Asit Kumar Chakraborty. Heterogeneous Phyto-Antibiotics and Other Future Therapeutics Against Multi-Drug Resistant Bacteria. Adv Biochem. 2019;7(2):34-50. doi: 10.11648/j.ab.20190702.11

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  • @article{10.11648/j.ab.20190702.11,
      author = {Asit Kumar Chakraborty},
      title = {Heterogeneous Phyto-Antibiotics and Other Future Therapeutics Against Multi-Drug Resistant Bacteria},
      journal = {Advances in Biochemistry},
      volume = {7},
      number = {2},
      pages = {34-50},
      doi = {10.11648/j.ab.20190702.11},
      url = {https://doi.org/10.11648/j.ab.20190702.11},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ab.20190702.11},
      abstract = {Phage therapy, enzybiotics, gene medicine and nanodrug-carriers are most vital components of modern research against multidrug-resistant bacteria. Genome evolutionoccurs due to environmental toxicities of various types where soil bacteria are in symbiosis with plant world who secret anti-metabolites against soil bacteria. Gut microbiome secrete vitamins required for our body where as intestinal cells synthesis interleukins and cytokines for symbiotic control of intestinal bacteria. Before the discovery of antibiotic in 1928, peoples trusted plant derived remedies against variety of illness described in Indian Sanskrit books like Charaka Samhita, Sasruta Samhita and Atharva Veda. AMR disease occurred due to repeated but uncontrolled use of antibiotics affecting gut microbiome who acquired many mdr genes in plasmids. We estimated that are 40% of all river and sea water borne bacteria were ampicillin and to lesser extent tetracycline, azithromycin, ciprofloxacin and streptomycin resistant. Where as amp, neo, tet, aac, cat, aph, aad, mcr-1, blaNDM-1, blaKPC-1, arr3, sul1, dhfr, inh, acrAB, mexAB, macAB and mtrCD were amplified with millions mutated isomers increasing all drugs MIC. We studied Indian medicinal plants and spices to get valuable cheap drugs where modified agriculture and/or tissue culture increased the drug concentration. We showed that MDR bacteria were sensitive to organic extract of Suregada multiflora roots, Cassia fistula bark, Jatropha gossypifolia roots as well as Indian spices Labanga and Derchini (MDR-Cure). TLC and HPLC purification as well as UV-VIS, MASS, NMR, FT-IR and XRD-Powder gave many distinct signatures of pure chemicals that also inhibited multidrug-resistant bacteria. Biological targets of 12 chemicals are under investigation targeting DNA Topoisomerase I, DNA Polymerase and RNA Polymerase of Escherichia coli. Indian Government has started Herbal Mission, Ganga Mission and various Plantation Programmes to curve MDR pathogenesis.},
     year = {2019}
    }
    

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  • TY  - JOUR
    T1  - Heterogeneous Phyto-Antibiotics and Other Future Therapeutics Against Multi-Drug Resistant Bacteria
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    AB  - Phage therapy, enzybiotics, gene medicine and nanodrug-carriers are most vital components of modern research against multidrug-resistant bacteria. Genome evolutionoccurs due to environmental toxicities of various types where soil bacteria are in symbiosis with plant world who secret anti-metabolites against soil bacteria. Gut microbiome secrete vitamins required for our body where as intestinal cells synthesis interleukins and cytokines for symbiotic control of intestinal bacteria. Before the discovery of antibiotic in 1928, peoples trusted plant derived remedies against variety of illness described in Indian Sanskrit books like Charaka Samhita, Sasruta Samhita and Atharva Veda. AMR disease occurred due to repeated but uncontrolled use of antibiotics affecting gut microbiome who acquired many mdr genes in plasmids. We estimated that are 40% of all river and sea water borne bacteria were ampicillin and to lesser extent tetracycline, azithromycin, ciprofloxacin and streptomycin resistant. Where as amp, neo, tet, aac, cat, aph, aad, mcr-1, blaNDM-1, blaKPC-1, arr3, sul1, dhfr, inh, acrAB, mexAB, macAB and mtrCD were amplified with millions mutated isomers increasing all drugs MIC. We studied Indian medicinal plants and spices to get valuable cheap drugs where modified agriculture and/or tissue culture increased the drug concentration. We showed that MDR bacteria were sensitive to organic extract of Suregada multiflora roots, Cassia fistula bark, Jatropha gossypifolia roots as well as Indian spices Labanga and Derchini (MDR-Cure). TLC and HPLC purification as well as UV-VIS, MASS, NMR, FT-IR and XRD-Powder gave many distinct signatures of pure chemicals that also inhibited multidrug-resistant bacteria. Biological targets of 12 chemicals are under investigation targeting DNA Topoisomerase I, DNA Polymerase and RNA Polymerase of Escherichia coli. Indian Government has started Herbal Mission, Ganga Mission and various Plantation Programmes to curve MDR pathogenesis.
    VL  - 7
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