Advances in Biochemistry
Volume 8, Issue 1, March 2020, Pages: 16-20
Received: Feb. 9, 2020;
Accepted: Feb. 26, 2020;
Published: Mar. 10, 2020
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Frank Uchenna Eneh, Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
Uchechukwu Chibuzo Ogbodo, Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
Chibuike Johnson Ogbu, Department of Applied Biochemistry, Nnamdi Azikiwe University, Awka, Anambra State, Nigeria
Hyperlipidemia has remained a major risk factor in the pathophysiology of atherosclerosis and other cardiovascular diseases and research efforts have been geared toward proffering remedies through diet and nutrition inherent in potent herbaceous plants. This study therefore investigated the effect of ethanol extract of the leaves of Colocasia esculenta on the serum lipid profile of male Albino rats as well as phytochemicals of ethnomedicinal importance. A total of 18 rats divided into three groups of 6 rats each were engaged in the investigation. The first group (baseline) was sacrificed after purchase; second group (control) was fed rat chow, and the third group (test) was fed a composite feed containing rat chow and 5% extract of C. esculenta leaves. The lipid profile total cholesterol (TC), triacylglycerols (TAG), Low Density Lipoprotein (LDL), High Density Lipoprotein (HDL) and Very Low Density Lipoprotein (VLDL) of the rats were assayed after 21 days of feeding and the result showed a significant difference (p<0.05) between the test and control groups for the lipids assayed. The extracts of C. esculenta reduced TC and LDL levels significantly (p<0.05) from 4.01±2.28 in control to 0.31±0.25mmol/L in the test for TC and then from 1.53±1.19 in the control to 0.07±0.04mmol/L in the test for LDL. This indicates that the leaves of this plant have a very high tendency to be protective against cardiovascular diseases. However, there was a marked increase in the TAG level of the test group (2.37±0.84mmol/L) compared to the control group (0.22±0.18mmol/L). This was nevertheless found to be not significant (p>0.05) but indicates the possibility for enhancement of TAG synthesis by the leaves of C. esculenta. Phytochemical screening showed a rich array of plant metabolites including flavonoids, saponins, tannins and alkaloids which may contribute to the hypolipidemic effects of ethanol extract of the plant. Further investigations may require identifying and characterizing the phytochemicals responsible for the overall role of the plant in folklore medicine.
Frank Uchenna Eneh,
Uchechukwu Chibuzo Ogbodo,
Chibuike Johnson Ogbu,
Lipid Lowering Potentials and Phytochemical Properties of Colocasia esculenta Leaf Extract in Rats, Advances in Biochemistry.
Vol. 8, No. 1,
2020, pp. 16-20.
WHO (2017). Fact sheet on Cardiovascular disease.
Xenouis, P. G. and Steiner, J. M. (2010). Lipid metabolism and hyperlipidemia in dogs. Vet. J. 183: 12-21.
Adams, K. F., Schatzkin, A., Harris, T. B., Kipnis, V., Mouw, T., Ballard-Barbash, R. et al. (2006). Overweight, obesity, and mortality in a large prospective cohort of persons 50 to 71 years old. N. Engl. J. Med. 355 (8): 763–78.
Pischon, T., Nöthlings, U. and Boeing, H. Obesity and cancer (2008). Proc. Nutr. Soc. 67 (2): 128–45.
Eneh, F. U., Ugochukwu, C. G. and Okoye, C. M. (2018). Effect of Ethanol extract of Curcubita pepo leaves on the Lipid Profile of Wistar Albino Rats. Asian J. Res. Biochem. 2 (4): 1-7.
Abuajah, C. I., Ogbonna, A. C. and Osuji, C. M. (2015). Functional components and medicinal properties of food: A review. J. Food Sci. Tech. 52: 2522-2529.
Krishnapriya, T. V. and Suganthi, A. (2017). Biochemical and phytochemical analysis of Colocasia esculenta (L.) Schott tubers. Int. J. Res. Pharma. & Pharmaceu. Sci. 2 (3): 21-25.
Yadav, M. Kushawaha, D. K. Chatterji, S. and Watal, G. (2017). Assessment of Antioxidant Activity and Phytochemical Screening of Colocasia esculenta Corm. Int. J. Pharma Sci. Res. 32: 1758-64.
Grindley, B. A., Omoruyi, F., Asemota, H. N. and Morrisona, A. (2002). Carbohydrate digestion and intestinal ATPases in streptozotocin-induced diabetic rats fed extract of yam (Dioscorea cayenesis) or dasheen (Colocasia esculenta). Nutr. Res. 22: 333-341.
Brown, A. C., Reitzenstein, J. E., Liu, J. and Jadus, M. R. (2005). The anti-Cancer effects of poi (Colocasia esculenta) on colonic adenocarcinoma cells in vitro. Physio. Res. 19 (9): 767-71.
Sakano, Y., Mutsuga, M., Tanaka, R., Suganuma, H., Inakuma, T., Tovoda, M. et al. (2005). Inhibition of Human Lanosterol synthase by the constituents of Colocasia esculenta (Taro). Biol. & Pharma. Bull. 28: 299-304.
Boban, P. T., Nambisan, B. and Sudhakaran, P. R. (2006). Hypolipidaemic effect of chemically different mucilages in rats: A comparative study. Brit. J. Nutr. 96: 1021-1029.
Shah, B. N., Nayak, B. S., Bhatt, S. P., Jalalpure, S. S. and Sheth, A. K. (2007). The anti-inflammatory activity of the leaves of Colocasia esculenta. Saudi Pharma. J. 15: 3-4.
Ufelle, S. A., Onyekwelu, K. C., Ghasi, S., Ezeh, C. O., Ezeh, R. C. and Esom, E. A. (2018). Effects of Colocasia esculenta leaf extract in anemic and normal Wistar rats. J. Med. Sci. 38: 102-106.
Azubuike, N. C., Okwuosa, C. N., Nwachukwu, D. C., Onyemelukwe, A. O., Onukwe, O. S., Chukwu, I. J. P., Orji, O., Orjiakor, N. P. and Achukwu, P. U. (2017). In vivo hepatoprotective studies on saponin and alkaloid rich fractions isolated from Colocasia esculenta (L. schott) leaves Pharmacol. Online 2: 66-74.
Keshav, A., Sharma, A. and Mazumdar, B. (2019). Phytochemical Analysis and Antioxidant Activity of Colocasia esculenta (L.) leaves. Int. J. Chem. & Mol. Eng. 13 (1): 20-23.
Kalariya, M., Parmar, S. and Sheth, N. (2010). Neuropharmacological activity of hydroalcoholic extract of leaves of Colocasia esculenta. Pharma. Biol. 201: 48: 1207-1212.
Sidhu, D. and Naugler, C. (2012). Fasting time and lipid levels in a community-based population: a cross-sectional study. Arch. Intern. Med. 172: 1707-1710.
Harborne, J. P. (1973). Phytochemical methods. London. Chapman and Hall Limited, pp. 49-88.
Das, U. N. (2006). Essential fatty acids: biochemistry, physiology and pathology. Biotech. J. 1 (4): 420-439.
Enkhmaa, B., Surampudi, P., Anuurad, E. and Berglund, L. (2000). Lifestyle Changes: Effect of Diet, Exercise, Functional Food, and Obesity Treatment on Lipids and Lipoproteins. [Updated 2018 Sep 11]. In: Feingold KR, Anawalt B, Boyce A, et al., editors. Endotext [Internet]. South Dartmouth (MA): MDText.com, Inc.
Bagri, P., Ali, M., Aeri, V., Bhowmik, M. and Sultana, S. (2009). Antidiabetic effect of Punica granatum flowers: effect on hyperlipidemia, pancreatic cells lipid peroxidation and antioxidant enzymes in experimental diabetes. Food & Chem. Toxic. 47: 50-54.
Subramani, S. and Casmir, C. A. (2002). Flavonoids and antioxidant activity of Georgia grown Vidalia onions. J. Agric. Food Chem. 50 (19): 5338-5342.
Okwu, D. E. (2001). Evaluation of the chemical composition of indigenous spices and flavoring agents. Glob. J. Pure Appl. Sci. 7 (3): 455-459.
Oakenfull, D. and Sidhu, G. (1990). Could saponins be a useful treatment for hypercholesterolemia. Euro. J. Clin. Nutr. 44: 79-88.
Walker, A. F., Marakis, G., Morris, A. P. and Robinson, P. A. (2002). Promising hypotensive effect of hawthorn extract: a randomized double-blind pilot study of mid, essential hypertension. Phytoth. Res. 16 (1): 48-54.
Wagner, K. H. and Elmadfa, I. (2003). Biological relevance of terpenoids. Overview focusing on mono-, di- and tertraterpenes. Anna. Nutr. Met. 47 (3): 95-106.
Kwiterovich, P. O. (2000). The metabolic pathways of high-density lipoprotein, low-density lipoprotein and triglycerides: a current review. Am. J. Cardiol. 12: 510.