Effect of L-Methionine Feeding on Serum Homocysteine and Glutathione Levels in Male and Female Wistar Rats
Advances in Biochemistry
Volume 8, Issue 1, March 2020, Pages: 21-25
Received: Feb. 19, 2020;
Accepted: Mar. 10, 2020;
Published: Mar. 23, 2020
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Shatha Ahmad Demerchi, School of Science and Technology, University of New England, Armidale, Australia; Technical College/Kirkuk, Northern Technical University, Kirkuk, Iraq
James Robert McFarlane, School of Science and Technology, University of New England, Armidale, Australia
Pierre Dominique Jean Moens, School of Science and Technology, University of New England, Armidale, Australia
Nicola King, School of Biomedical Sciences, University of Plymouth, Plymouth, UK
Homocysteine (Hcy) is a critical indicator of cardiovascular disease. High levels of Hcy have now been recognised as a risk factor for the development of a wide range of diseases. Hyperhomocysteinemia (Hhcy) can be induced by methionine or Hcy supplementation. On the other hand, Glutathione (GSH) is a major antioxidant in the body and also an important compound for oxidative defence. It is composed of 3 amino acids: cysteine, glutamate, and glycine. Interestingly, methionine is also a crucial compound in GSH synthesis. This study aims to assess the impact of 1% L-methionine feeding (10 or 30 weeks) on the body weight and serum Hcy and GSH levels of young adult (16 weeks) and middle-aged (36 weeks) Wistar rats of both sexes. Serum was analysed for Hcy and reduced GSH levels by liquid chromatography mass spectrometry (LCMS) in response to 1% L-methionine feeding. One percent L-methionine feeding decreased body weight in all conditions investigated, although this only reached significance in males after 10 weeks supplementation and females after 30 weeks supplementation. It also induced a significant increase in the serum Hcy levels of male Wistar rats, whilst having no significant effect on Hcy serum levels in female rats. Finally, we also observed a small increase in serum GSH levels in female Wistar rats but no change in serum GSH levels in the males. These results suggest that methionine feeding affects body weight homeostasis and alters by products of methionine catabolism.
Shatha Ahmad Demerchi,
James Robert McFarlane,
Pierre Dominique Jean Moens,
Effect of L-Methionine Feeding on Serum Homocysteine and Glutathione Levels in Male and Female Wistar Rats, Advances in Biochemistry.
Vol. 8, No. 1,
2020, pp. 21-25.
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