Enzyme Profiles of <i>Pleurotus</i> HK-37 During Mycelia Vegetative Growth and Fruiting on Solid Sisal Waste Fractions Supplemented with Cow Manure

Prosper Raymond; Anthony Manoni Mshandete; Amelia Kajumulo Kivaisi

doi:doi:10.11648/j.ab.20150305.12

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Enzyme Profiles of Pleurotus HK-37 During Mycelia Vegetative Growth and Fruiting on Solid Sisal Waste Fractions Supplemented with Cow Manure

Prosper Raymond, Anthony Manoni Mshandete, Amelia Kajumulo Kivaisi

Published in Advances in Biochemistry (Volume 3, Issue 5)

Received: 3 September 2015 Accepted: 16 September 2015 Published: 26 September 2015

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Abstract

The utilization of agro-industrial residues to cultivate edible mushrooms is of great environmental importance. However, the use of lignocellulose as carbon source depends on the capacity of the mushroom to produce lignocellulolytic enzymes and to secrete them to the extracellular substrate. Thus, the profile of lignocellulolytic enzymes produced during different phases of cultivation of Pleurotus HK-37 on sisal waste fractions supplemented with cow dung manure was determined. Mushroom cultivation was performed in plastic bags using substrates formulated by mixing various proportions of sisal leaf residues and sisal boles and supplementing with cow dung manure on dry weight basis. A total of three hydrolytic (carboxymethyl cellulase, pectinase and xylanase) and two oxidative (laccase and lignin peroxidase) enzymes produced by Pleurotus HK-37 were analyzed. Among these enzymes assayed, laccase was found to be predominant and highly expressed. After 30 days of incubation, its activity was 158.75 ± 7.66 Ug^-1wet spent mushroom substrate (SMS) on 30% supplemented sisal leaf decortication residues: sisal boles (25:75) substrate formulation. The highest lignin peroxidase activity observed was 4.01 ± 1.12 Ug^-1wet SMS during full mycelia colonization on unsupplemented sisal leaf decortication residues: sisal boles (25:75) substrate formulation. Meanwhile, for the hydrolytic enzymes; the highest carboxymethyl cellulase activity (5.45 Ug-1wet SMS) was observed on unsupplemeted sisal leaf decortication residues: sisal boles (75:25) substrate formulation 50 days after of substrate inoculation, that of xylanase (3.73 ± 0.98 Ug^-1wet SMS) was found on 10% supplemented sisal leaf decortication residues: sisal boles (0:100) substrate formulation after 20 days and that of pectinase (8.28 ± 2.14 Ug^-1wet SMS) was observed 20 days after substrate inoculation on 30% supplemented sisal leaf decortication residues: sisal boles (100:0) substrate formulation. The present investigation indicates the utilization of solid sisal wastes as support-substrate for production of both edible mushrooms and extracellular enzymes during solid state fermentation; it also provides an alternative approach and value-addition to these agrowaste residues.

Published in	Advances in Biochemistry (Volume 3, Issue 5)
DOI	10.11648/j.ab.20150305.12
Page(s)	57-65
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Oxidative Enzymes, Hydrolytic Enzymes, Pleurotus HK-37, Sisal Wastes

References

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Prosper Raymond, Anthony Manoni Mshandete, Amelia Kajumulo Kivaisi. (2015). Enzyme Profiles of Pleurotus HK-37 During Mycelia Vegetative Growth and Fruiting on Solid Sisal Waste Fractions Supplemented with Cow Manure. Advances in Biochemistry, 3(5), 57-65. https://doi.org/10.11648/j.ab.20150305.12

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Prosper Raymond; Anthony Manoni Mshandete; Amelia Kajumulo Kivaisi. Enzyme Profiles of Pleurotus HK-37 During Mycelia Vegetative Growth and Fruiting on Solid Sisal Waste Fractions Supplemented with Cow Manure. Adv. Biochem. 2015, 3(5), 57-65. doi: 10.11648/j.ab.20150305.12

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Prosper Raymond, Anthony Manoni Mshandete, Amelia Kajumulo Kivaisi. Enzyme Profiles of Pleurotus HK-37 During Mycelia Vegetative Growth and Fruiting on Solid Sisal Waste Fractions Supplemented with Cow Manure. Adv Biochem. 2015;3(5):57-65. doi: 10.11648/j.ab.20150305.12

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@article{10.11648/j.ab.20150305.12,
  author = {Prosper Raymond and Anthony Manoni Mshandete and Amelia Kajumulo Kivaisi},
  title = {Enzyme Profiles of Pleurotus HK-37 During Mycelia Vegetative Growth and Fruiting on Solid Sisal Waste Fractions Supplemented with Cow Manure},
  journal = {Advances in Biochemistry},
  volume = {3},
  number = {5},
  pages = {57-65},
  doi = {10.11648/j.ab.20150305.12},
  url = {https://doi.org/10.11648/j.ab.20150305.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20150305.12},
  abstract = {The utilization of agro-industrial residues to cultivate edible mushrooms is of great environmental importance. However, the use of lignocellulose as carbon source depends on the capacity of the mushroom to produce lignocellulolytic enzymes and to secrete them to the extracellular substrate. Thus, the profile of lignocellulolytic enzymes produced during different phases of cultivation of Pleurotus HK-37 on sisal waste fractions supplemented with cow dung manure was determined. Mushroom cultivation was performed in plastic bags using substrates formulated by mixing various proportions of sisal leaf residues and sisal boles and supplementing with cow dung manure on dry weight basis. A total of three hydrolytic (carboxymethyl cellulase, pectinase and xylanase) and two oxidative (laccase and lignin peroxidase) enzymes produced by Pleurotus HK-37 were analyzed. Among these enzymes assayed, laccase was found to be predominant and highly expressed. After 30 days of incubation, its activity was 158.75 ± 7.66 Ug-1wet spent mushroom substrate (SMS) on 30% supplemented sisal leaf decortication residues: sisal boles (25:75) substrate formulation. The highest lignin peroxidase activity observed was 4.01 ± 1.12 Ug-1wet SMS during full mycelia colonization on unsupplemented sisal leaf decortication residues: sisal boles (25:75) substrate formulation. Meanwhile, for the hydrolytic enzymes; the highest carboxymethyl cellulase activity (5.45 Ug-1wet SMS) was observed on unsupplemeted sisal leaf decortication residues: sisal boles (75:25) substrate formulation 50 days after of substrate inoculation, that of xylanase (3.73 ± 0.98 Ug-1wet SMS) was found on 10% supplemented sisal leaf decortication residues: sisal boles (0:100) substrate formulation after 20 days and that of pectinase (8.28 ± 2.14 Ug-1wet SMS) was observed 20 days after substrate inoculation on 30% supplemented sisal leaf decortication residues: sisal boles (100:0) substrate formulation. The present investigation indicates the utilization of solid sisal wastes as support-substrate for production of both edible mushrooms and extracellular enzymes during solid state fermentation; it also provides an alternative approach and value-addition to these agrowaste residues.},
 year = {2015}
}

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TY  - JOUR
T1  - Enzyme Profiles of Pleurotus HK-37 During Mycelia Vegetative Growth and Fruiting on Solid Sisal Waste Fractions Supplemented with Cow Manure
AU  - Prosper Raymond
AU  - Anthony Manoni Mshandete
AU  - Amelia Kajumulo Kivaisi
Y1  - 2015/09/26
PY  - 2015
N1  - https://doi.org/10.11648/j.ab.20150305.12
DO  - 10.11648/j.ab.20150305.12
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 57
EP  - 65
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20150305.12
AB  - The utilization of agro-industrial residues to cultivate edible mushrooms is of great environmental importance. However, the use of lignocellulose as carbon source depends on the capacity of the mushroom to produce lignocellulolytic enzymes and to secrete them to the extracellular substrate. Thus, the profile of lignocellulolytic enzymes produced during different phases of cultivation of Pleurotus HK-37 on sisal waste fractions supplemented with cow dung manure was determined. Mushroom cultivation was performed in plastic bags using substrates formulated by mixing various proportions of sisal leaf residues and sisal boles and supplementing with cow dung manure on dry weight basis. A total of three hydrolytic (carboxymethyl cellulase, pectinase and xylanase) and two oxidative (laccase and lignin peroxidase) enzymes produced by Pleurotus HK-37 were analyzed. Among these enzymes assayed, laccase was found to be predominant and highly expressed. After 30 days of incubation, its activity was 158.75 ± 7.66 Ug-1wet spent mushroom substrate (SMS) on 30% supplemented sisal leaf decortication residues: sisal boles (25:75) substrate formulation. The highest lignin peroxidase activity observed was 4.01 ± 1.12 Ug-1wet SMS during full mycelia colonization on unsupplemented sisal leaf decortication residues: sisal boles (25:75) substrate formulation. Meanwhile, for the hydrolytic enzymes; the highest carboxymethyl cellulase activity (5.45 Ug-1wet SMS) was observed on unsupplemeted sisal leaf decortication residues: sisal boles (75:25) substrate formulation 50 days after of substrate inoculation, that of xylanase (3.73 ± 0.98 Ug-1wet SMS) was found on 10% supplemented sisal leaf decortication residues: sisal boles (0:100) substrate formulation after 20 days and that of pectinase (8.28 ± 2.14 Ug-1wet SMS) was observed 20 days after substrate inoculation on 30% supplemented sisal leaf decortication residues: sisal boles (100:0) substrate formulation. The present investigation indicates the utilization of solid sisal wastes as support-substrate for production of both edible mushrooms and extracellular enzymes during solid state fermentation; it also provides an alternative approach and value-addition to these agrowaste residues.
VL  - 3
IS  - 5
ER  -

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Author Information

Prosper Raymond

Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, University of Dar es Salaam, Dar es Salaam, Tanzania
Anthony Manoni Mshandete

Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, University of Dar es Salaam, Dar es Salaam, Tanzania
Amelia Kajumulo Kivaisi

Department of Molecular Biology and Biotechnology, College of Natural and Applied Sciences, University of Dar es Salaam, Dar es Salaam, Tanzania

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Prosper Raymond, Anthony Manoni Mshandete, Amelia Kajumulo Kivaisi. (2015). Enzyme Profiles of Pleurotus HK-37 During Mycelia Vegetative Growth and Fruiting on Solid Sisal Waste Fractions Supplemented with Cow Manure. Advances in Biochemistry, 3(5), 57-65. https://doi.org/10.11648/j.ab.20150305.12

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Prosper Raymond; Anthony Manoni Mshandete; Amelia Kajumulo Kivaisi. Enzyme Profiles of Pleurotus HK-37 During Mycelia Vegetative Growth and Fruiting on Solid Sisal Waste Fractions Supplemented with Cow Manure. Adv. Biochem. 2015, 3(5), 57-65. doi: 10.11648/j.ab.20150305.12

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AMA Style

Prosper Raymond, Anthony Manoni Mshandete, Amelia Kajumulo Kivaisi. Enzyme Profiles of Pleurotus HK-37 During Mycelia Vegetative Growth and Fruiting on Solid Sisal Waste Fractions Supplemented with Cow Manure. Adv Biochem. 2015;3(5):57-65. doi: 10.11648/j.ab.20150305.12

Copy | Download

@article{10.11648/j.ab.20150305.12,
  author = {Prosper Raymond and Anthony Manoni Mshandete and Amelia Kajumulo Kivaisi},
  title = {Enzyme Profiles of Pleurotus HK-37 During Mycelia Vegetative Growth and Fruiting on Solid Sisal Waste Fractions Supplemented with Cow Manure},
  journal = {Advances in Biochemistry},
  volume = {3},
  number = {5},
  pages = {57-65},
  doi = {10.11648/j.ab.20150305.12},
  url = {https://doi.org/10.11648/j.ab.20150305.12},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20150305.12},
  abstract = {The utilization of agro-industrial residues to cultivate edible mushrooms is of great environmental importance. However, the use of lignocellulose as carbon source depends on the capacity of the mushroom to produce lignocellulolytic enzymes and to secrete them to the extracellular substrate. Thus, the profile of lignocellulolytic enzymes produced during different phases of cultivation of Pleurotus HK-37 on sisal waste fractions supplemented with cow dung manure was determined. Mushroom cultivation was performed in plastic bags using substrates formulated by mixing various proportions of sisal leaf residues and sisal boles and supplementing with cow dung manure on dry weight basis. A total of three hydrolytic (carboxymethyl cellulase, pectinase and xylanase) and two oxidative (laccase and lignin peroxidase) enzymes produced by Pleurotus HK-37 were analyzed. Among these enzymes assayed, laccase was found to be predominant and highly expressed. After 30 days of incubation, its activity was 158.75 ± 7.66 Ug-1wet spent mushroom substrate (SMS) on 30% supplemented sisal leaf decortication residues: sisal boles (25:75) substrate formulation. The highest lignin peroxidase activity observed was 4.01 ± 1.12 Ug-1wet SMS during full mycelia colonization on unsupplemented sisal leaf decortication residues: sisal boles (25:75) substrate formulation. Meanwhile, for the hydrolytic enzymes; the highest carboxymethyl cellulase activity (5.45 Ug-1wet SMS) was observed on unsupplemeted sisal leaf decortication residues: sisal boles (75:25) substrate formulation 50 days after of substrate inoculation, that of xylanase (3.73 ± 0.98 Ug-1wet SMS) was found on 10% supplemented sisal leaf decortication residues: sisal boles (0:100) substrate formulation after 20 days and that of pectinase (8.28 ± 2.14 Ug-1wet SMS) was observed 20 days after substrate inoculation on 30% supplemented sisal leaf decortication residues: sisal boles (100:0) substrate formulation. The present investigation indicates the utilization of solid sisal wastes as support-substrate for production of both edible mushrooms and extracellular enzymes during solid state fermentation; it also provides an alternative approach and value-addition to these agrowaste residues.},
 year = {2015}
}

Copy | Download

TY  - JOUR
T1  - Enzyme Profiles of Pleurotus HK-37 During Mycelia Vegetative Growth and Fruiting on Solid Sisal Waste Fractions Supplemented with Cow Manure
AU  - Prosper Raymond
AU  - Anthony Manoni Mshandete
AU  - Amelia Kajumulo Kivaisi
Y1  - 2015/09/26
PY  - 2015
N1  - https://doi.org/10.11648/j.ab.20150305.12
DO  - 10.11648/j.ab.20150305.12
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 57
EP  - 65
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20150305.12
AB  - The utilization of agro-industrial residues to cultivate edible mushrooms is of great environmental importance. However, the use of lignocellulose as carbon source depends on the capacity of the mushroom to produce lignocellulolytic enzymes and to secrete them to the extracellular substrate. Thus, the profile of lignocellulolytic enzymes produced during different phases of cultivation of Pleurotus HK-37 on sisal waste fractions supplemented with cow dung manure was determined. Mushroom cultivation was performed in plastic bags using substrates formulated by mixing various proportions of sisal leaf residues and sisal boles and supplementing with cow dung manure on dry weight basis. A total of three hydrolytic (carboxymethyl cellulase, pectinase and xylanase) and two oxidative (laccase and lignin peroxidase) enzymes produced by Pleurotus HK-37 were analyzed. Among these enzymes assayed, laccase was found to be predominant and highly expressed. After 30 days of incubation, its activity was 158.75 ± 7.66 Ug-1wet spent mushroom substrate (SMS) on 30% supplemented sisal leaf decortication residues: sisal boles (25:75) substrate formulation. The highest lignin peroxidase activity observed was 4.01 ± 1.12 Ug-1wet SMS during full mycelia colonization on unsupplemented sisal leaf decortication residues: sisal boles (25:75) substrate formulation. Meanwhile, for the hydrolytic enzymes; the highest carboxymethyl cellulase activity (5.45 Ug-1wet SMS) was observed on unsupplemeted sisal leaf decortication residues: sisal boles (75:25) substrate formulation 50 days after of substrate inoculation, that of xylanase (3.73 ± 0.98 Ug-1wet SMS) was found on 10% supplemented sisal leaf decortication residues: sisal boles (0:100) substrate formulation after 20 days and that of pectinase (8.28 ± 2.14 Ug-1wet SMS) was observed 20 days after substrate inoculation on 30% supplemented sisal leaf decortication residues: sisal boles (100:0) substrate formulation. The present investigation indicates the utilization of solid sisal wastes as support-substrate for production of both edible mushrooms and extracellular enzymes during solid state fermentation; it also provides an alternative approach and value-addition to these agrowaste residues.
VL  - 3
IS  - 5
ER  -

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