Voluntary Running Enhances Hippocampal Proliferation by Increasing Hippocampal NGF, BDNF, and IGF-1

Bo Xu; Xianliang Zhang; Chenghui Song; Fei Liang; Li Zhang

doi:doi:10.11648/j.ab.20170501.11

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Voluntary Running Enhances Hippocampal Proliferation by Increasing Hippocampal NGF, BDNF, and IGF-1

Bo Xu, Xianliang Zhang, Chenghui Song, Fei Liang, Li Zhang

Published in Advances in Biochemistry (Volume 5, Issue 1)

Received: 4 January 2017 Accepted: 25 January 2017 Published: 4 March 2017

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Abstract

Neurotrophic factors and growth factors are known to have positive effects on hippocampal proliferation. However, previous findings on the effects of these factors on exercise-induced hippocampal proliferation are inconsistent. In this study, we investigated the effects of voluntary running on hippocampal proliferation and the expression levels of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and insulin-like growth factor 1 (IGF-1) in hippocampus. Twenty-four male C57BL/6 mice were randomly divided into four groups: control groups 1 and 2 (C1, n = 6; C2, n = 6) and running groups 1 and 2 (R1, n = 6; R2, n = 6). Mice from C1 and C2 were placed individually in standard cages for eight weeks; mice from R1 and R2 were housed individually in cages with a running wheel for eight weeks. At the fifth week, C1 and R1 mice were injected with BrdU for 7 consecutive days. On the first day of the ninth week, the C1 and R1 mice were anaesthetized and hippocampal neuronal proliferation was assessed using immunohistochemistry; the mice in C2 and R2 were sacrificed to test the expression levels of NGF, BDNF, and IGF-1 mRNA in hippocampus using RT-PCR. The results indicated that voluntary running significantly increased the number of BrdU-positive cells in the dentate gyrus (DG), and significantly upregulated the levels of NGF, BDNF, and IGF-1 mRNA in the hippocampus. In conclusion, our findings indicated that hippocampal proliferation was enhanced by voluntary running, which was associated with increased NGF, BDNF, and IGF-1 mRNA levels in the hippocampus.

Published in	Advances in Biochemistry (Volume 5, Issue 1)
DOI	10.11648/j.ab.20170501.11
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Voluntary Exercise, Hippocampal Proliferation, NGF, BDNF, IGF-1

References

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APA Style

Bo Xu, Xianliang Zhang, Chenghui Song, Fei Liang, Li Zhang. (2017). Voluntary Running Enhances Hippocampal Proliferation by Increasing Hippocampal NGF, BDNF, and IGF-1. Advances in Biochemistry, 5(1), 1-6. https://doi.org/10.11648/j.ab.20170501.11

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ACS Style

Bo Xu; Xianliang Zhang; Chenghui Song; Fei Liang; Li Zhang. Voluntary Running Enhances Hippocampal Proliferation by Increasing Hippocampal NGF, BDNF, and IGF-1. Adv. Biochem. 2017, 5(1), 1-6. doi: 10.11648/j.ab.20170501.11

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AMA Style

Bo Xu, Xianliang Zhang, Chenghui Song, Fei Liang, Li Zhang. Voluntary Running Enhances Hippocampal Proliferation by Increasing Hippocampal NGF, BDNF, and IGF-1. Adv Biochem. 2017;5(1):1-6. doi: 10.11648/j.ab.20170501.11

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@article{10.11648/j.ab.20170501.11,
  author = {Bo Xu and Xianliang Zhang and Chenghui Song and Fei Liang and Li Zhang},
  title = {Voluntary Running Enhances Hippocampal Proliferation by Increasing Hippocampal NGF, BDNF, and IGF-1},
  journal = {Advances in Biochemistry},
  volume = {5},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ab.20170501.11},
  url = {https://doi.org/10.11648/j.ab.20170501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20170501.11},
  abstract = {Neurotrophic factors and growth factors are known to have positive effects on hippocampal proliferation. However, previous findings on the effects of these factors on exercise-induced hippocampal proliferation are inconsistent. In this study, we investigated the effects of voluntary running on hippocampal proliferation and the expression levels of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and insulin-like growth factor 1 (IGF-1) in hippocampus. Twenty-four male C57BL/6 mice were randomly divided into four groups: control groups 1 and 2 (C1, n = 6; C2, n = 6) and running groups 1 and 2 (R1, n = 6; R2, n = 6). Mice from C1 and C2 were placed individually in standard cages for eight weeks; mice from R1 and R2 were housed individually in cages with a running wheel for eight weeks. At the fifth week, C1 and R1 mice were injected with BrdU for 7 consecutive days. On the first day of the ninth week, the C1 and R1 mice were anaesthetized and hippocampal neuronal proliferation was assessed using immunohistochemistry; the mice in C2 and R2 were sacrificed to test the expression levels of NGF, BDNF, and IGF-1 mRNA in hippocampus using RT-PCR. The results indicated that voluntary running significantly increased the number of BrdU-positive cells in the dentate gyrus (DG), and significantly upregulated the levels of NGF, BDNF, and IGF-1 mRNA in the hippocampus. In conclusion, our findings indicated that hippocampal proliferation was enhanced by voluntary running, which was associated with increased NGF, BDNF, and IGF-1 mRNA levels in the hippocampus.},
 year = {2017}
}

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TY  - JOUR
T1  - Voluntary Running Enhances Hippocampal Proliferation by Increasing Hippocampal NGF, BDNF, and IGF-1
AU  - Bo Xu
AU  - Xianliang Zhang
AU  - Chenghui Song
AU  - Fei Liang
AU  - Li Zhang
Y1  - 2017/03/04
PY  - 2017
N1  - https://doi.org/10.11648/j.ab.20170501.11
DO  - 10.11648/j.ab.20170501.11
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20170501.11
AB  - Neurotrophic factors and growth factors are known to have positive effects on hippocampal proliferation. However, previous findings on the effects of these factors on exercise-induced hippocampal proliferation are inconsistent. In this study, we investigated the effects of voluntary running on hippocampal proliferation and the expression levels of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and insulin-like growth factor 1 (IGF-1) in hippocampus. Twenty-four male C57BL/6 mice were randomly divided into four groups: control groups 1 and 2 (C1, n = 6; C2, n = 6) and running groups 1 and 2 (R1, n = 6; R2, n = 6). Mice from C1 and C2 were placed individually in standard cages for eight weeks; mice from R1 and R2 were housed individually in cages with a running wheel for eight weeks. At the fifth week, C1 and R1 mice were injected with BrdU for 7 consecutive days. On the first day of the ninth week, the C1 and R1 mice were anaesthetized and hippocampal neuronal proliferation was assessed using immunohistochemistry; the mice in C2 and R2 were sacrificed to test the expression levels of NGF, BDNF, and IGF-1 mRNA in hippocampus using RT-PCR. The results indicated that voluntary running significantly increased the number of BrdU-positive cells in the dentate gyrus (DG), and significantly upregulated the levels of NGF, BDNF, and IGF-1 mRNA in the hippocampus. In conclusion, our findings indicated that hippocampal proliferation was enhanced by voluntary running, which was associated with increased NGF, BDNF, and IGF-1 mRNA levels in the hippocampus.
VL  - 5
IS  - 1
ER  -

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Author Information

Bo Xu

Key Laboratory of Adolescent Health Assessment and Exercise Intervention of Ministry of Education, East China Normal University, Shanghai, China
Xianliang Zhang

College of Physical Education, Shandong University, Jinan, China
Chenghui Song

Department of Neuroscience, the Scripps Research Institute, Jupiter, Florida, USA
Fei Liang

School of Physical Education & Health Care, East China Normal University, Shanghai, China
Li Zhang

Division of Physical Education, China University of Mining & Technology, Beijing, China

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APA Style

Bo Xu, Xianliang Zhang, Chenghui Song, Fei Liang, Li Zhang. (2017). Voluntary Running Enhances Hippocampal Proliferation by Increasing Hippocampal NGF, BDNF, and IGF-1. Advances in Biochemistry, 5(1), 1-6. https://doi.org/10.11648/j.ab.20170501.11

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ACS Style

Bo Xu; Xianliang Zhang; Chenghui Song; Fei Liang; Li Zhang. Voluntary Running Enhances Hippocampal Proliferation by Increasing Hippocampal NGF, BDNF, and IGF-1. Adv. Biochem. 2017, 5(1), 1-6. doi: 10.11648/j.ab.20170501.11

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AMA Style

Bo Xu, Xianliang Zhang, Chenghui Song, Fei Liang, Li Zhang. Voluntary Running Enhances Hippocampal Proliferation by Increasing Hippocampal NGF, BDNF, and IGF-1. Adv Biochem. 2017;5(1):1-6. doi: 10.11648/j.ab.20170501.11

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@article{10.11648/j.ab.20170501.11,
  author = {Bo Xu and Xianliang Zhang and Chenghui Song and Fei Liang and Li Zhang},
  title = {Voluntary Running Enhances Hippocampal Proliferation by Increasing Hippocampal NGF, BDNF, and IGF-1},
  journal = {Advances in Biochemistry},
  volume = {5},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.ab.20170501.11},
  url = {https://doi.org/10.11648/j.ab.20170501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ab.20170501.11},
  abstract = {Neurotrophic factors and growth factors are known to have positive effects on hippocampal proliferation. However, previous findings on the effects of these factors on exercise-induced hippocampal proliferation are inconsistent. In this study, we investigated the effects of voluntary running on hippocampal proliferation and the expression levels of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and insulin-like growth factor 1 (IGF-1) in hippocampus. Twenty-four male C57BL/6 mice were randomly divided into four groups: control groups 1 and 2 (C1, n = 6; C2, n = 6) and running groups 1 and 2 (R1, n = 6; R2, n = 6). Mice from C1 and C2 were placed individually in standard cages for eight weeks; mice from R1 and R2 were housed individually in cages with a running wheel for eight weeks. At the fifth week, C1 and R1 mice were injected with BrdU for 7 consecutive days. On the first day of the ninth week, the C1 and R1 mice were anaesthetized and hippocampal neuronal proliferation was assessed using immunohistochemistry; the mice in C2 and R2 were sacrificed to test the expression levels of NGF, BDNF, and IGF-1 mRNA in hippocampus using RT-PCR. The results indicated that voluntary running significantly increased the number of BrdU-positive cells in the dentate gyrus (DG), and significantly upregulated the levels of NGF, BDNF, and IGF-1 mRNA in the hippocampus. In conclusion, our findings indicated that hippocampal proliferation was enhanced by voluntary running, which was associated with increased NGF, BDNF, and IGF-1 mRNA levels in the hippocampus.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Voluntary Running Enhances Hippocampal Proliferation by Increasing Hippocampal NGF, BDNF, and IGF-1
AU  - Bo Xu
AU  - Xianliang Zhang
AU  - Chenghui Song
AU  - Fei Liang
AU  - Li Zhang
Y1  - 2017/03/04
PY  - 2017
N1  - https://doi.org/10.11648/j.ab.20170501.11
DO  - 10.11648/j.ab.20170501.11
T2  - Advances in Biochemistry
JF  - Advances in Biochemistry
JO  - Advances in Biochemistry
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2329-0862
UR  - https://doi.org/10.11648/j.ab.20170501.11
AB  - Neurotrophic factors and growth factors are known to have positive effects on hippocampal proliferation. However, previous findings on the effects of these factors on exercise-induced hippocampal proliferation are inconsistent. In this study, we investigated the effects of voluntary running on hippocampal proliferation and the expression levels of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), and insulin-like growth factor 1 (IGF-1) in hippocampus. Twenty-four male C57BL/6 mice were randomly divided into four groups: control groups 1 and 2 (C1, n = 6; C2, n = 6) and running groups 1 and 2 (R1, n = 6; R2, n = 6). Mice from C1 and C2 were placed individually in standard cages for eight weeks; mice from R1 and R2 were housed individually in cages with a running wheel for eight weeks. At the fifth week, C1 and R1 mice were injected with BrdU for 7 consecutive days. On the first day of the ninth week, the C1 and R1 mice were anaesthetized and hippocampal neuronal proliferation was assessed using immunohistochemistry; the mice in C2 and R2 were sacrificed to test the expression levels of NGF, BDNF, and IGF-1 mRNA in hippocampus using RT-PCR. The results indicated that voluntary running significantly increased the number of BrdU-positive cells in the dentate gyrus (DG), and significantly upregulated the levels of NGF, BDNF, and IGF-1 mRNA in the hippocampus. In conclusion, our findings indicated that hippocampal proliferation was enhanced by voluntary running, which was associated with increased NGF, BDNF, and IGF-1 mRNA levels in the hippocampus.
VL  - 5
IS  - 1
ER  -

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