Advances in Biochemistry

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Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats

Received: 18 May 2017    Accepted: 31 May 2017    Published: 13 July 2017
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Abstract

Widely used in Ivory Coast and sub-Saharan Africa in traditional medicine as anti-inflammatory, antioxidant, laxative and purgative, Erythrococca anomala (Euphorbiaceae), is an annual plant. In this work, the aim was to evaluate the Hepatoprotective activity of the aqueous and hydroethanolic extracts of the leaves. Carbon Tetrachloride (CCl4) was used to induce hepatotoxicity in rats. This hepatotoxic effect causes a significant increase in the levels of liver enzymes and serum proteins. Hepatoprotective activity was assessed by assaying liver enzymes such as serum transaminases (alanine aminotransferase and aspartate aminotransferase) and proteins by spectrophotometric and electrophoretic techniques. When administered orally as a preventive and curative dose of 100 and 200 mg / kg body weight, the extracts significantly reduce significantly as silymarin, the reference hepatoprotective substance at 100 mg / kg bw, hepatotoxicity induced by Carbon tetrachloride (CCL4) at a dose of 5 mg / kg bw after a significant reduction in liver enzyme levels, globulins and a significant increase in albumin. The possible mechanism of this hepatoprotective activity of the extracts may be due to the action of the antioxidants such as the flavonoids, the phenols present in the extracts.

DOI 10.11648/j.ab.20170504.12
Published in Advances in Biochemistry (Volume 5, Issue 4, August 2017)
Page(s) 61-66
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Erythrococca anomala, Silymarin, Carbon Tetrachloride, Hepatotoxicity, Hepatoprotective

References
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[4] Miezan BAP, Okpekon AT, Yapi HF, Bony FN, Gbassi G, Assi YJ. Chemical component and acute toxicity study of Erythrococca anomala (Euphorbiaceae). Asian Journal of Biomedical and Pharmaceutical Sciences. 2016; 6(57): 4-8. 5.
[5] Oladele GM, Abatan MO, Olukynle JO, Okediran BS. Anti-inflammatory and analgesic effect of aqueous leafextracts of Gomphrenacelosioides and Momordicacharinda. J Nat. Sat. Sci. Engr. Tech. 2009; 8(2): 1-8.6.
[6] Patrice M BA, Yapi HF, Opkekon AT, Bony FN, Kouakou-Siransy G, Kouakou YébouéKF. Evaluation of anti-inflammatory activities of Erythrococca anomalaaqueous and ethanolic extracts from leaves in rat. 14(2): 1-7, 2016; IJBCRR. 28385.
[7] Miezan BAP, Okpekon AT, Koukou SL, Droucoula GC, Yapi HF. Evaluation of Antioxidant Activities of Erythrococca anomala Aqueous and Hydroethanolic Extracts. Journal ofBiotechnology and Biochemistry. Volume3, (2017), 113-118.
[8] Adjanohoun EJ, Ake-Assi L. Contribution au recensement des plantes médicinales de Côte d’Ivoire. Centre National de Floristique de l’Université Nationale de Côte d’Ivoire, Tome 1, 23-30. Lavoisier, Paris. 1979; 895.
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[11] Hegde K., A. B. Joshia. (2009). Hepatoprotective effect of Carissa carandas root extract against CCl4 and paracetamol induced hepatic oxidative stress. India J. Exp. Biol., 47: 660-667.
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[13] Krishna K. L., Mruthunjaya K., Patel J. A. (2010). Antioxidant and hepatoprotective potential of stem methanolic extract of JusticiagendarussaBurm. Int. J. Pharma. 67: 2-80.
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Author Information
  • Laboratory of Biochemical Pharmacodynamics, UFR Biosciences, Felix Houphouet Boigny University, Abidjan, Ivory Coast

  • Department of Pharmacology, Clinical and Therapeutic Pharmacy UFR Pharmaceutical and Biological Sciences, Felix Houphouet Boigny University, Abidjan, Ivory Coast

  • Laboratory of Biochemical Pharmacodynamics, UFR Biosciences, Felix Houphouet Boigny University, Abidjan, Ivory Coast

  • Department of Pharmacology, Clinical and Therapeutic Pharmacy UFR Pharmaceutical and Biological Sciences, Felix Houphouet Boigny University, Abidjan, Ivory Coast

  • Laboratory of Biochemical Pharmacodynamics, UFR Biosciences, Felix Houphouet Boigny University, Abidjan, Ivory Coast

  • Laboratory of Biochemical Pharmacodynamics, UFR Biosciences, Felix Houphouet Boigny University, Abidjan, Ivory Coast

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    Miezan Bile Aka Patrice, Kouakou Sylvain Landry, Droucoula Guillaume Cyril, Kouakou-Syransy Giselle, Koffi Akissi Jeanne, et al. (2017). Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats. Advances in Biochemistry, 5(4), 61-66. https://doi.org/10.11648/j.ab.20170504.12

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    ACS Style

    Miezan Bile Aka Patrice; Kouakou Sylvain Landry; Droucoula Guillaume Cyril; Kouakou-Syransy Giselle; Koffi Akissi Jeanne, et al. Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats. Adv. Biochem. 2017, 5(4), 61-66. doi: 10.11648/j.ab.20170504.12

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    AMA Style

    Miezan Bile Aka Patrice, Kouakou Sylvain Landry, Droucoula Guillaume Cyril, Kouakou-Syransy Giselle, Koffi Akissi Jeanne, et al. Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats. Adv Biochem. 2017;5(4):61-66. doi: 10.11648/j.ab.20170504.12

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  • @article{10.11648/j.ab.20170504.12,
      author = {Miezan Bile Aka Patrice and Kouakou Sylvain Landry and Droucoula Guillaume Cyril and Kouakou-Syransy Giselle and Koffi Akissi Jeanne and Yapi Houphouet Felix},
      title = {Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats},
      journal = {Advances in Biochemistry},
      volume = {5},
      number = {4},
      pages = {61-66},
      doi = {10.11648/j.ab.20170504.12},
      url = {https://doi.org/10.11648/j.ab.20170504.12},
      eprint = {https://download.sciencepg.com/pdf/10.11648.j.ab.20170504.12},
      abstract = {Widely used in Ivory Coast and sub-Saharan Africa in traditional medicine as anti-inflammatory, antioxidant, laxative and purgative, Erythrococca anomala (Euphorbiaceae), is an annual plant. In this work, the aim was to evaluate the Hepatoprotective activity of the aqueous and hydroethanolic extracts of the leaves. Carbon Tetrachloride (CCl4) was used to induce hepatotoxicity in rats. This hepatotoxic effect causes a significant increase in the levels of liver enzymes and serum proteins. Hepatoprotective activity was assessed by assaying liver enzymes such as serum transaminases (alanine aminotransferase and aspartate aminotransferase) and proteins by spectrophotometric and electrophoretic techniques. When administered orally as a preventive and curative dose of 100 and 200 mg / kg body weight, the extracts significantly reduce significantly as silymarin, the reference hepatoprotective substance at 100 mg / kg bw, hepatotoxicity induced by Carbon tetrachloride (CCL4) at a dose of 5 mg / kg bw after a significant reduction in liver enzyme levels, globulins and a significant increase in albumin. The possible mechanism of this hepatoprotective activity of the extracts may be due to the action of the antioxidants such as the flavonoids, the phenols present in the extracts.},
     year = {2017}
    }
    

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    T1  - Evaluation of Hepatoprotective Activities Aqueous and Hydroethanolic Extracts from the Leaves of Erythrococca anomala in Rats
    AU  - Miezan Bile Aka Patrice
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    AU  - Droucoula Guillaume Cyril
    AU  - Kouakou-Syransy Giselle
    AU  - Koffi Akissi Jeanne
    AU  - Yapi Houphouet Felix
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    DO  - 10.11648/j.ab.20170504.12
    T2  - Advances in Biochemistry
    JF  - Advances in Biochemistry
    JO  - Advances in Biochemistry
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    PB  - Science Publishing Group
    SN  - 2329-0862
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    AB  - Widely used in Ivory Coast and sub-Saharan Africa in traditional medicine as anti-inflammatory, antioxidant, laxative and purgative, Erythrococca anomala (Euphorbiaceae), is an annual plant. In this work, the aim was to evaluate the Hepatoprotective activity of the aqueous and hydroethanolic extracts of the leaves. Carbon Tetrachloride (CCl4) was used to induce hepatotoxicity in rats. This hepatotoxic effect causes a significant increase in the levels of liver enzymes and serum proteins. Hepatoprotective activity was assessed by assaying liver enzymes such as serum transaminases (alanine aminotransferase and aspartate aminotransferase) and proteins by spectrophotometric and electrophoretic techniques. When administered orally as a preventive and curative dose of 100 and 200 mg / kg body weight, the extracts significantly reduce significantly as silymarin, the reference hepatoprotective substance at 100 mg / kg bw, hepatotoxicity induced by Carbon tetrachloride (CCL4) at a dose of 5 mg / kg bw after a significant reduction in liver enzyme levels, globulins and a significant increase in albumin. The possible mechanism of this hepatoprotective activity of the extracts may be due to the action of the antioxidants such as the flavonoids, the phenols present in the extracts.
    VL  - 5
    IS  - 4
    ER  - 

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