Background: Preeclampsia is a serious disorder affecting the mother. It may be responsible for more than 50,000 maternal deaths worldwide each year. It is a major killer of both mother and fetus. Aim: To demonstrate the morphological changes of human placental tissue in pre-eclamptic pregnancies compared with the normal by using histological and immunohistochemical techniques. Materials & Methods: Twenty pregnant women were included in this study. 10 of them were diagnosed as having moderate pre-eclampsia and considered as the pre-eclamptic (PE) group, while 10 women were clinically normal and considered as the control group. Placental specimens were obtained after either vaginal delivery or caesarian section and processed for H&E and Masson's trichrome stains, as well as for immunohistochemical stains for eNOS and HIF-1α. Morphometric measurement of number of syncytial knots, area % of collagen fibers, eNOS and HIF-1α.were done followed by statistical analysis. Results: PE group revealed thickened wall of fetal blood capillaries, crowded degenerating villi with decreased intervillous spaces, intravillous and perivillous fibrinoid deposition. Numerous syncytial knots and exfoliated trophoblast cells were also observed. There was thickened layer of subchorionic fibrinoid. Increased area% of collagen fibers, HIF-1α. immunoreactivity and decreased area% of eNOS immunoexpression were found. Conclusions: As evidenced by the previous results, this study concluded various placental changes detected by histological and histochemical techniques. The findings have clarified a significant correlation between eNOS and HIF-1α immunoexpression and pre-eclampsia. Whether this relation is causal or effect relationship, this may need further investigations for better management and avoidance of consequences of pre-eclampsia.
| Published in | Cell Biology (Volume 2, Issue 6) |
| DOI | 10.11648/j.cb.20140206.13 |
| Page(s) | 72-80 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Pre-Eclampsia, eNOS, HIF-1α, Histological, Immunohistochemical
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APA Style
Noha Abdellatif Ibrahim, Doaa Mabrouk Khaled. (2014). Histological and Immunohistochemical Study on Human Placental Tissue in Normal Pregnancy and Preeclampsia. Cell Biology, 2(6), 72-80. https://doi.org/10.11648/j.cb.20140206.13
ACS Style
Noha Abdellatif Ibrahim; Doaa Mabrouk Khaled. Histological and Immunohistochemical Study on Human Placental Tissue in Normal Pregnancy and Preeclampsia. Cell Biol. 2014, 2(6), 72-80. doi: 10.11648/j.cb.20140206.13
AMA Style
Noha Abdellatif Ibrahim, Doaa Mabrouk Khaled. Histological and Immunohistochemical Study on Human Placental Tissue in Normal Pregnancy and Preeclampsia. Cell Biol. 2014;2(6):72-80. doi: 10.11648/j.cb.20140206.13
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Download@article{10.11648/j.cb.20140206.13,
author = {Noha Abdellatif Ibrahim and Doaa Mabrouk Khaled},
title = {Histological and Immunohistochemical Study on Human Placental Tissue in Normal Pregnancy and Preeclampsia},
journal = {Cell Biology},
volume = {2},
number = {6},
pages = {72-80},
doi = {10.11648/j.cb.20140206.13},
url = {https://doi.org/10.11648/j.cb.20140206.13},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20140206.13},
abstract = {Background: Preeclampsia is a serious disorder affecting the mother. It may be responsible for more than 50,000 maternal deaths worldwide each year. It is a major killer of both mother and fetus. Aim: To demonstrate the morphological changes of human placental tissue in pre-eclamptic pregnancies compared with the normal by using histological and immunohistochemical techniques. Materials & Methods: Twenty pregnant women were included in this study. 10 of them were diagnosed as having moderate pre-eclampsia and considered as the pre-eclamptic (PE) group, while 10 women were clinically normal and considered as the control group. Placental specimens were obtained after either vaginal delivery or caesarian section and processed for H&E and Masson's trichrome stains, as well as for immunohistochemical stains for eNOS and HIF-1α. Morphometric measurement of number of syncytial knots, area % of collagen fibers, eNOS and HIF-1α.were done followed by statistical analysis. Results: PE group revealed thickened wall of fetal blood capillaries, crowded degenerating villi with decreased intervillous spaces, intravillous and perivillous fibrinoid deposition. Numerous syncytial knots and exfoliated trophoblast cells were also observed. There was thickened layer of subchorionic fibrinoid. Increased area% of collagen fibers, HIF-1α. immunoreactivity and decreased area% of eNOS immunoexpression were found. Conclusions: As evidenced by the previous results, this study concluded various placental changes detected by histological and histochemical techniques. The findings have clarified a significant correlation between eNOS and HIF-1α immunoexpression and pre-eclampsia. Whether this relation is causal or effect relationship, this may need further investigations for better management and avoidance of consequences of pre-eclampsia.},
year = {2014}
}
TY - JOUR T1 - Histological and Immunohistochemical Study on Human Placental Tissue in Normal Pregnancy and Preeclampsia AU - Noha Abdellatif Ibrahim AU - Doaa Mabrouk Khaled Y1 - 2014/12/31 PY - 2014 N1 - https://doi.org/10.11648/j.cb.20140206.13 DO - 10.11648/j.cb.20140206.13 T2 - Cell Biology JF - Cell Biology JO - Cell Biology SP - 72 EP - 80 PB - Science Publishing Group SN - 2330-0183 UR - https://doi.org/10.11648/j.cb.20140206.13 AB - Background: Preeclampsia is a serious disorder affecting the mother. It may be responsible for more than 50,000 maternal deaths worldwide each year. It is a major killer of both mother and fetus. Aim: To demonstrate the morphological changes of human placental tissue in pre-eclamptic pregnancies compared with the normal by using histological and immunohistochemical techniques. Materials & Methods: Twenty pregnant women were included in this study. 10 of them were diagnosed as having moderate pre-eclampsia and considered as the pre-eclamptic (PE) group, while 10 women were clinically normal and considered as the control group. Placental specimens were obtained after either vaginal delivery or caesarian section and processed for H&E and Masson's trichrome stains, as well as for immunohistochemical stains for eNOS and HIF-1α. Morphometric measurement of number of syncytial knots, area % of collagen fibers, eNOS and HIF-1α.were done followed by statistical analysis. Results: PE group revealed thickened wall of fetal blood capillaries, crowded degenerating villi with decreased intervillous spaces, intravillous and perivillous fibrinoid deposition. Numerous syncytial knots and exfoliated trophoblast cells were also observed. There was thickened layer of subchorionic fibrinoid. Increased area% of collagen fibers, HIF-1α. immunoreactivity and decreased area% of eNOS immunoexpression were found. Conclusions: As evidenced by the previous results, this study concluded various placental changes detected by histological and histochemical techniques. The findings have clarified a significant correlation between eNOS and HIF-1α immunoexpression and pre-eclampsia. Whether this relation is causal or effect relationship, this may need further investigations for better management and avoidance of consequences of pre-eclampsia. VL - 2 IS - 6 ER -
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