Spermidine Enhances Activities of Detoxification Enzymes in Onion (Allium cepa L.) Seedlings Under Short Term Salinity
Cell Biology
Volume 4, Issue 3, May 2016, Pages: 18-23
Received: Sep. 26, 2016; Accepted: Oct. 10, 2016; Published: Nov. 3, 2016
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Tanjina Islam, Molecular Breeding Lab, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh; Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh
Md. Ismail Hossain, Department of Horticulture, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh
M. S. Rahaman, Department of Environmental Science, Stamford University, Dhaka, Bangladesh
Md. Motiar Rohman, Molecular Breeding Lab, Bangladesh Agricultural Research Institute, Gazipur, Bangladesh
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In plant, glyoxalases [glyoxalase I (Gly-I, EC: and glyoxalase II (Gly-II, EC:] and glutathione S-transferase (GST, EC: are major detoxification enzymes. On the other hand, spermidine (Spd) is important polyamine (PA) with significant role which interacts with stress protection mechanisms functioning in common against different types of stress. In this study, exogenous Spd was applied on onion seedlings to investigate its protective role through regulation of glyoxalase and GST activities. Continuous increase was observed in the content of methylglyoxal (MG) in onion leaves under salinity, and at 7 day of stress, MG contents increased by 260% over control. Application of Spd reduced the MG contents in saline treated seedlings through increasing glyoxalase mediated detoxification by 21 and 48% at 1 and 3 day of stress, respectively. Salinity increased Gly-I and Gly-II activities which was further increased by Spd upto 3 day of stress. On the other hand, salinity increased GST activity by 14, 55, 93 and 109% over control at 1, 3, 5 and 7 day, respectively. Application of Spd increased the activity in stressed seedlings at 3 day of stress while 21% higher activity was found. However, after 3 days, both glyoxalases and GST activities in Spd treated seedlings decreased and became almost similar to those in drought stressed seedlings without Spd. Considering the results, application of Spd in onion seedlings improved tolerance for short period of salinity.
Spermidine, Glyoxalases, GST, Onion Seedlings
To cite this article
Tanjina Islam, Md. Ismail Hossain, M. S. Rahaman, Md. Motiar Rohman, Spermidine Enhances Activities of Detoxification Enzymes in Onion (Allium cepa L.) Seedlings Under Short Term Salinity, Cell Biology. Vol. 4, No. 3, 2016, pp. 18-23. doi: 10.11648/j.cb.20160403.11
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Demiral T, Türkan I. 2005. Comparative lipid peroxidation, antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance. Environ. Exp. Botany 53 (3): 247-257.
Mandhania S, Madan S, Sawhney V. 2006. Antioxidant defense mechanism under salt stress in wheat seedlings. Biol. Plant 50 (2): 227-231.
Misra N, Gupta AK. 2006. Interactive effects of sodium and calcium on proline metabolism in salt tolerant green gram cultivar. Am. J. Plant Physiol 1 (1): 1-12.
Hasegawa PM, Bressan RA, Zhu JK, Bohnert, HJ. 2000. Plant cellular and molecular responses to high salinity. Annu. Rev. Plant Physiol. Plant Mol. Biol 51: 463-499.
Apel K, Hirt H. 2004. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant. Biol 55: 373-399.
Yadav SK, Singla-Pareek SL, Reddy MK, Sopory SK. 2005a. Transgenic tobacco plants overexpressing glyoxalase enzymes resist an increase in methylglyoxal and maintain higher reduced glutathione levels under salinity stress. FEBS Lett 579 (27): 6265-6271.
Yadav SK, Singla-Pareek SL, Ray M, Reddy MK, Sopory SK. 2005b. Methylglyoxal levels in plants under salinity stress are dependent on glyoxalase I and glutathione. Biochem. Biophys. Res. Commun 337 (1): 61-67.
Noctor G, Mhamdi A, Chaouch S, Han Y, Neukermans J, Marquez-Garcia B, Queval G, Foyer CH. 2012. Glutathione in plants: an integrated overview. Plant Cell Environ 35 (2): 454-484.
Noctor G, Foyer CH. 1998. Ascorbate and glutathione: keeping active oxygen under control. Annu. Rev. Plant Physiol. Plant Mol. Biol 49: 249-79.
Choudhury S, Panda P, Sahoo L, Panda SK. 2013. Reactive oxygen species signaling in plants under abiotic stress. Plant Signal. Behav 8 (4): e23681.
Edwards R, Dixon DP. 2005. Plant glutathione transferases. In glutathione transferases and gamma-glutamyltranspeptidases (Sies, H. and Packer, L., eds). San Diego, CA: Elsevier Academic Press Inc, pp. 81169-186.
Dixon DP, Steel PG, Edwards R. 2011. Roles for glutathione transferases in antioxidant recycling, Plant Signaling & Behavior 6 (8): 1223-1227.
Chi Y, Cheng Y, Vanitha J, Kumar N, Ramamoorthy R, Ramachandran S, Jiang SY. 2011. Expansion mechanisms and functional divergence of the glutathione S-transferase family in Sorghum and other higher plants. DNA Res 18 (1): 1-16.
Chen JH, Jiang HW, Hsieh EJ, Chen HY, Chien CT, Hsieh HL, Lin TP. 2012. Drought and salt stress tolerance of an Arabidopsis glutathione S-transferase U17 knockout mutant are attributed to the combined effect of glutathione and abscisic acid. Plant Physiol 158 (1): 340-351.
Zagorchev L, Seal CE, Kranner I, Odjakova M. 2013. A Central role for thiols in plant tolerance to abiotic stress. Int. J. Mol. Sci 14: 7405-7432.
Marrs KA, Alfenito MR, Lloyd AM, Walbot V. 1995. A glutathione S-transferase involved in vacuolar transfer encoded by the maize gene Bronze-2. Nature 375 (6530): 397-400.
Mueller LA, Goodman CD, Silady RA, Walbot V. 2000. AN9, a petunia glutathione S-transferaserequired for anthocyanin sequestration, is a flavonoid-binding protein. Plant Physiol 123 (4): 1561-1570.
Kitamura S, Shikazono N, Tanaka A. 2004. TRANSPARENT TESTA 19 is involved in the accumulation of both anthocyanins and proanthocyanidins in Arabidopsis. Plant J 37 (1): 104-114.
Alfenito MR, Souer E, Goodman CD, Buell R, Mol J, Koes R, Walbot V. 1998. Functional complementation of anthocyanin sequestration in the vacuole by widely divergent glutathione S-transferases. Plant Cell 10 (7): 1135-1149.
Edwards R, Dixon DP, Walbot V. 2000. Plant glutathione S-transferases: enzymes with multiple functions in sickness and in health. Trends Plant Sci 5: 193-198.
Mustafiz A, Sahoo KK, Singla-Pareek SL, Sopory SK. 2010. Metabolic engineering of glyoxalase pathway for enhancing stress tolerance in plants. Methods Mol. Biol 639: 95-118.
Singla-Pareek SL, Yadav SK, Pareek A, Reddy MK, Sopory SK. 2008. Enhancing salt tolerance in a crop plant by overexpression of glyoxalase II. Transgenic Res 17 (2): 171-180.
Saxena M, Bisht R, Roy SD, Sopory SK, Bhalla-Sarin N. 2005. Cloning and characterization of a mitochondrial glyoxalase II from Brassica juncea that is upregulated by NaCl, Zn and ABA. Biochem. Biophys. Res. Commun 336 (3): 813-819.
Saxena M, Deb Roy S, Singla-Pareek S-L, Sopory SK, Bhalla-Sarin N. 2011. Overexpression of the glyoxalase II gene leads to enhanced salinity tolerance in Brassica juncea. The Open Plant Sci J 5: 23-28.
Martin-Tanguy J. 2001. Metabolism and function of polyamines in plants: recent development (new approaches). Plant Growth Regul 34: 135-148.
Zhao, H. and Yang, H. 2008. Exogenous polyamines alleviate the lipid peroxidation induced by cadmium chloride stress in Malushupehensis. Rehd. SciHort 116: 442-7.
Bouchereau A, Aziz A, Larher F, Martin-Tanguy J. 1999. Polyamines and environmental challenges: recent development. Plant Sci 140: 103-125.
Alca´zar R, Marco F, Cuevas JC, Patron M, Ferrando A, Carrasco P, Tiburcio AF, Altabella T. 2006. Involvement of polyamines in plant response to abiotic stress. Biotech. Lett 28: 1867-1876.
Krishnamurthy R, Bhagwat KA. 1989. Polyamines as modulators of salt tolerance in rice cultivars. Plant Physiol 91: 500-504.
Langebartels C, Kerner K, Leonardi S, Schraudner M, Trost M, Heller W, Andermann HJ. 1991. Biochemical plant responses to ozone. I. Differential induction of polyamine and ethylene biosynthesis in tobacco. Plant Physiol 95: 882-889.
Kurepa J, Smalle J, Montagu MV, Inzé D. 1998. Polyamines and paraquat toxicity in Arabidopsis thaliana. Plant Cell Physiol 39: 987-992.
Roy M, Ghosh B. 1996. Polyamines, both common and uncommon, under heat stress in rice (Oryza sativa) callus. Plant Physiol 98: 196-200.
Shen W, Nada K, Tachibana S. 2000 Involvement of polyamines in the chilling tolerance of cucumber cultivars. Plant Physiol 124: 431-439.
He L, Nada K, Kasukabe Y, Tachibana S. 2002. Enhanced susceptibility of photosynthesis to low-temperature photoinhibition due to interruption of chill-induced increase of S-adenosylmethionine decarboxylase activity in leaves of spinach (Spinacia oleracea L.). Plant Cell Physiol 43: 196-206.
Besford RT, Richardson CM, Campos JL, Tiburcio AF. 1993. Effect of polyamines on stabilization of molecular complexes in thylakoid membranes of osmotically stressed oat leaves. Planta 189: 201-206.
Nada K, Iwatani E, Doi T, Tachibana S. 2004. Effect of putrescine pretreatment to roots on growth and lactate metabolism in the root of tomato (Lycopersicum esculentum Mill.) under root-zone hypoxia. J. Jpn. Soc. Hortic. Sci 73: 337-339
Roychoudhury A, Basu S, Sengupta DN. 2011. Amelioration of salinity stress by exogenously applied spermidine or spermine in three varieties of indica rice differing in their level of salt tolerance. J. Plant Physiol 168: 317-328.
Kasukabe Y, He L, Nada K, Misawa S, Ihara I, Tachibana S. 2004. Overexpression of spermidinesynthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress- regulated genes in transgenic Arabidopsis thaliana. Plant Cell Physiol 45 (6): 712-722.
Hossain MD, Rohman MM, Fujita M. 2007. A Comparative investigation of glutathione S-transferases, glyoxalase-I and alliinase activities in different vegetable crops. J. Crop. Sci. Biotechnol 10: 21-28.
Bradford MM. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem 72: 248-254.
Rohman MM, Uddin S, Fujita M. 2010. Up-regulation of onion bulb glutathione S-transferases (GSTs) by abiotic stresses: A comparative study between two differently sensitive GSTs to their physiological inhibitors. Plant Omics J 3: 28-34.
Principato GB, Rosi G, Talesa V, Govannini E, Uolila L. 1987. Purification and characterization of two forms of glyoxalase II from rat liver and brain of Wistar rats. Biochem. Biophys. Acta 911: 349-355.
Rohman MM, Begum S, Talukder MZA, Akhi AH, Amiruzzaman M, Ahsan AFMS and Hossain Z. 2016. Drought sensitive maize inbred shows more oxidative damage and higher ROS scavenging enzymes, but not glyoxalases than a tolerant one at seedling stage. Plant Omics J 9 (4): 220-232.
Noctor G, Gomez LA, Vanacker H, Foyer CH. 2002. Interactions between biosynthesis, comparmentation and transport in the control of glutathione homeostasis and signaling. J. Exp. Bot 53: 1283-1304.
Hoque MA, Okuma E, Banu MNA, Nakamura Y, Shimoishi Y, Murata Y. 2007. Exogenous proline mitigates the detrimental effects of salt stress more than exogenous betaine by increasing antioxidant enzyme activities. J. Plant Physiol 164 (5): 553-61.
Hoque MA, Banu MNA, Nakamura Y, Shimoishi Y, Murata Y. 2008. Proline and glycinebetaine enhance antioxidant defense and methylglyoxal detoxification systems and reduce NaCl-induced damage in cultured tobacco cells. J. Plant Physiol 165 (8): 813-824.
Shalata A, Neumann PM. 2001. Exogenous ascorbic acid (vitamin C) increases resistance to salt stress and reduces lipid peroxidation. J. Exp. Bot 52: 2207-2211.
Mittova V, Theodoulou FL, Kiddle G, Gomez L, Volokita M, Tal M. 2003. Co-ordinate induction of glutathione biosynthesis and glutathione metabolizing enzymes is correlated with salt tolerance. FEBS Lett 554: 417-421.
Dixon DP, Skipsey M, Edwards R. 2010. Roles for glutathione transferases in plant secondary metabolism. Phytochem 71: 338-350.
Fini A, Brunetti C, Ferdinando MD, Ferrini F, Tattini. 2011. Stress-induced flavonoid biosynthesis and the antioxidant machinery of plants. Plant Signal. Behav 6 (5): 709-711.
Rohman MM, Hossain MD, Suzuki T, Takada G, Fujita M. 2009. Quercetin-4'-glucoside: a physiological inhibitor of the activities of dominant glutathione S-transferases in onion (Allium cepa L.) bulb. Acta. Plant Physiol 31 (2): 301-309.
Seppanen MM, Cardi T, Hyokki MB, Pehu E. 2000. Characterisation and expression of cold-induced glutathione S-transferase in freezing tolerant Solanumcommersonii, sensitive S. tuberosum and their interspecific somatic hybrids. Plant Sci 153 (2): 125-133.
Moons A. 2003. Osgstu 3 and osgtu 4, encoding tau class glutathione S-transferases, are heavy metal- and hypoxic stress-induced and differentially salt stress-responsive in rice roots. FEBS Lett 553 (3): 427-432.
Kiyosue T, Yamaguchi-Shinozaki K, Shinozaki K. 1993. Characterization of two cDNAs (ERD11 and ERD13) for hydration-inducible genes that encode putative glutathione S-transferases in Arabidopsis thaliana L. FEBS Lett 335 (2): 189-192.
Bianchi MW, Roux C, Vartanian N. 2002. Drought regulation of GST8, encoding the Arabidopsis homologue of ParC/Nt107 glutathione transferase/peroxidase. Plant Physiol 116 (1): 96-105.
Vollenweider S, Weber H, Stolz S, Chetelat A, Farmer EE. 2000. Fatty acid ketodienes and fatty acid ketotrienes: Michael addition acceptors that accumulate in wounded and diseased Arabidopsis leaves. Plant J 24 (4): 467-476.
Mouch F, Dudler R. 1993. Differential induction of distinct glutathione-S-transferase of wheat by xenobiotics and by pathogen attack. Plant Physiol 102: 1193-1201.
Zhou J, Goldsbrough PB. 1993. An Arabidopsis gene with homology to glutathione S-transferases is regulated by ethylene. Plant Mol. Biol 22: 517-523.
Chen W, Chao G, Singh KB. 1996. The promoter of a H2O2 inducible, Arabidopsis glutathione S-transferase gene contains closely linked OBF- and OBP1-binding sites. Plant J 10 (6): 955-966.
Gronwald JW, Plaisance KL. 1998. Isolation and characterization of glutathione S-transferase isozymes from sorghum. Plant Physiol 117 (3): 877-892.
Marrs KA. 1996. The functions and regulation of glutathione S-transferases in plants. Annu. Rev. Plant Physiol. Plant Mol. Biol 47: 127-158.
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