Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells
Volume 5, Issue 1, January 2017, Pages: 1-7
Received: Jan. 31, 2017;
Accepted: Feb. 20, 2017;
Published: Mar. 4, 2017
Views 2113 Downloads 131
Jian Tu, Department of Pharmacology, University of South China, Hengyang, China
Ting Xiong, Department of Pharmacology, University of South China, Hengyang, China
Shujing Teng, Department of Pharmacology, University of South China, Hengyang, China
Zhigang Zhou, Department of Anesthesiology, University of South China, Hengyang, China
Xiaoyong Lei, Department of Pharmacology, University of South China, Hengyang, China
Shengsong Tang, Department of Pharmacology, University of South China, Hengyang, China
Macrophage colony-stimulating factor (M-CSF), also named colony-stimulating factor-1 (CSF-1), plays an important role in the process of proliferation and differentiation of the monocyte/macrophage lineage cells. Commonly, it is not easy to measure the expression of cellular M-CSF. However, recent studies have shown that M-CSF can be expressed at a high level in the cytoplasm and nuclei of some kinds of malignant tumors, which related to the poor prognosis. To explore the role and mechanism of nuclear M-CSF, in the present study we constructed the pCMV/nuc/M-CSF vector and transfected it into human HeLa nuclei. The results from our previous study indicated, M-CSF was stably expressed in HeLa nuclei, which were used as a model to determine the nuclear effects of M-CSF. There was a higher percentage of replicating nuclei in the transfected pCMV/nuc/M-CSF HeLa cells both in phase G1 and S. According to the data from the cell doubling time, antisense oligonucleotides and the experiments of the transplanted tumor in nude mice, nuclear M-CSF could promote the cell proliferation of HeLa cells both in vivo and in vitro. In conclusion, nuclear M-CSF could accelerate DNA replication and cell proliferation of cervical carcinoma.
Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells, Cell Biology.
Vol. 5, No. 1,
2017, pp. 1-7.
Kasamatsu E, Cubilla AL, Alemany L, et al. Type-specific human papillomavirus distribution in invasive cervical carcinomas in Paraguay. A study of 432 cases. J Med Virol, 2012, 84: 1628~1635.
Pandey S, Chandravati. Autophagy in cervical cancer: an emerging therapeutic target. Asian Pac J Cancer Prev, 2012, 13: 4867~4871.
Ławicki S, Będkowska GE, Gacuta-Szumarska E, et al. Pretreatment plasma levels and diagnostic utility of hematopoietic cytokines in cervical cancer or cervical intraepithelial neoplasia patients. Folia Histochem Cytobiol, 2012, 50: 213~219.
Beirão BC, Raposo T, Pang LY, et al. Canine mammary cancer cells direct macrophages toward an intermediate activation state between M1/M2. BMC Vet Res, 2015, 11: 151.
Bellora F, Castriconi R, Doni A, et al. M-CSF induces the expression of a membrane-bound form of IL-18 in a subset of human monocytes differentiating in vitro toward macrophages. Eur J Immunol, 2012, 42: 1618~1626.
Liu W, Xu GZ, Jiang CH, et al. Expression of macrophage colony-stimulating factor (M-CSF) and its receptor in streptozotocin-induced diabetic rats. Curr Eye Res, 2009, 34: 123~133.
Itoh K, Udagawa N, Matsuzaki K, et al. Importance of membrane- or matrix-associated forms of M-CSF and RANKL/ODF in osteoclastogenesis supported by SaOS-4/3 cells expressing recombinant PTH/PTHrP receptors. J Bone Miner Res, 2000, 15: 1766~1775.
Booker BE, Clark RS, Pellom ST, et al. Interleukin-34 induces monocytic-like differentiation in leukemia cell lines. Int J Biochem Mol Biol, 2015; 6 (1): 1~16.
Ławicki S, Mroczko B, Omyła J, et al. Macrophage colony-stimulating factor (M-CSF) as a candidate for the tumor marker of breast cancer. Pol Arch Med Wewn 2003, 109: 597~602.
Lawicki S, Bedkowska E, Gacuta-Szumarska E, et al. The plasma levels and diagnostic utility of stem cell factor (SCF) and macrophage-colony stimulating factor (M-CSF) in cervical cancer patients. Pol Merkur Lekarski, 2008, 25: 38~42.
Espinosa I, Catasus L, D' Angelo E, et al. Stromal signatures in endometrioid endometrial carcinomas. Mod Pathol, 2014, 27 (4): 631~639.
Kirma N, Hammes LS, Liu YG, et al. Elevated expression of the oncogene c-fms and its ligand, the macrophage colony-stimulating factor-1, in cervical cancer and the role of transforming growth factor-beta1 in inducing c-fms expression. Cancer Res, 2007, 67: 1918~1926.
Powell JA, Thomas D, Barry EF, et al. Expression profiling of a hemopoietic cell survival transcriptome implicates osteopontin as a functional prognostic factor in AML. Blood, 2009, 114 (23): 4859~4870.
Bourguignon LY, Singleton PA, Zhu H, et al. Hyaluronan-mediated CD44 interaction with RhoGEF and Rho kinase promotes Grb2-associated binder-1 phosphorylation and phosphatidylinositol 3-kinase signaling leading to cytokine (macrophage-colony stimulating factor) production and breast tumor progression. J Biol Chem, 2003, 278: 29420~29434.
Newa M, Bhandari KH, Tang L, et al. Antibody-mediated "universal" osteoclast targeting platform using calcitonin as a model drug. Pharm Res, 2011, 28: 1131~1143.
Bedkowska GE, Ławicki S, Szmitkowski M. Molecular markers of carcinogenesis in the diagnostics of cervical cancer. Postepy Hig Med Dosw (Online), 2009, 63: 99~105.
Mancino AT, Klimberg VS, Yamamoto M, et al. Breast cancer increases osteoclastogenesis by secreting M-CSF and upregulating RANKL in stromal cells. J Surg Res 2001, 100: 18-24.
Tu J, Teng SJ, Zhang XH, et al. Construction and identification of M-CSF stably expressed in the cellular nuclear. Chinese Journal of Pathophysiology, 2009, 25: 622~624.
Cao ZY, Zhang B, Rao Q, et al. Effects of nuclear-presenting -macrophage colony-stimulating factor on the process of malignancy. Int J Hematol, 2003, 78: 87~89.
Darzynkiewicz Z, Halicka HD, Zhao H, et al. Cell synchronization by inhibitors of DNA replication induces replication stress and DNA damage response: analysis by flow cytometry. Methods Mol Biol, 2011, 761: 85~96.
Smith AM, Gibbons HM, Oldfield RL, et al. M-CSF increases proliferation and phagocytosis while modulating receptor and transcription factor expression in adult human microglia. J Neuroinflammation, 2013, 10: 85.
Curry JM, Eubank TD, Roberts RD, et al. M-CSF signals through the MAPK/ERK pathway via Sp1 to induce VEGF production and induces angiogenesis in vivo. PLoS One, 2008, 3: e3405.
Johansson AS, Lidén J, Okret S, et al. Effects of ethanol on cytokine generation and NFkappaB activity in human lung epithelial cell. Biochem Pharmacol, 2005, 70: 545~551.
Lu SY, Wan HC, Li M, et al. Subcellular localization of Mitf in monocytic cells. Histochem Cell Biol, 2010, 133: 651~658.
Li X, Vradii D, Gutierrez S, et al. Subnuclear targeting of Runx1 is required for synergistic activation of the myeloid specific M-CSF receptor promoter by PU. 1. J Cell Biochem, 2005, 96: 795~809.
Tuteja N, Tran NQ, Dang HQ, et al. Plant MCM proteins: role in DNA replication and beyond. Plant Mol Biol, 2011, 77: 537~545.
Bauerschmidt C, Pollok S, Kremmer E, et al. Interactions of human Cdc45 with the Mcm2-7 complex, the GINS complex, and DNA polymerases delta and epsilon during S phase. Genes Cells, 2007, 12: 745~758.