Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells
Cell Biology
Volume 5, Issue 1, January 2017, Pages: 1-7
Received: Jan. 31, 2017; Accepted: Feb. 20, 2017; Published: Mar. 4, 2017
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Jian Tu, Department of Pharmacology, University of South China, Hengyang, China
Ting Xiong, Department of Pharmacology, University of South China, Hengyang, China
Shujing Teng, Department of Pharmacology, University of South China, Hengyang, China
Zhigang Zhou, Department of Anesthesiology, University of South China, Hengyang, China
Xiaoyong Lei, Department of Pharmacology, University of South China, Hengyang, China
Shengsong Tang, Department of Pharmacology, University of South China, Hengyang, China
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Macrophage colony-stimulating factor (M-CSF), also named colony-stimulating factor-1 (CSF-1), plays an important role in the process of proliferation and differentiation of the monocyte/macrophage lineage cells. Commonly, it is not easy to measure the expression of cellular M-CSF. However, recent studies have shown that M-CSF can be expressed at a high level in the cytoplasm and nuclei of some kinds of malignant tumors, which related to the poor prognosis. To explore the role and mechanism of nuclear M-CSF, in the present study we constructed the pCMV/nuc/M-CSF vector and transfected it into human HeLa nuclei. The results from our previous study indicated, M-CSF was stably expressed in HeLa nuclei, which were used as a model to determine the nuclear effects of M-CSF. There was a higher percentage of replicating nuclei in the transfected pCMV/nuc/M-CSF HeLa cells both in phase G1 and S. According to the data from the cell doubling time, antisense oligonucleotides and the experiments of the transplanted tumor in nude mice, nuclear M-CSF could promote the cell proliferation of HeLa cells both in vivo and in vitro. In conclusion, nuclear M-CSF could accelerate DNA replication and cell proliferation of cervical carcinoma.
Microphage Colony-Stimulating Factor (M-CSF), HeLa Nuclei, DNA Replication, Cell Proliferation
To cite this article
Jian Tu, Ting Xiong, Shujing Teng, Zhigang Zhou, Xiaoyong Lei, Shengsong Tang, Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells, Cell Biology. Vol. 5, No. 1, 2017, pp. 1-7. doi: 10.11648/j.cb.20170501.11
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This article is an open access article distributed under the Creative Commons Attribution License ( which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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