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The Effect of Supporting Electrolyte on Ion Permeability Through Red Blood Cell Membrane

Received: 23 February 2017    Accepted: 14 March 2017    Published: 21 March 2017
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Abstract

This study was aimed at providing information on how Na sulphate, K sulphate, Ca sulphate and Mg sulphate supporting electrolytes affect the permeation of hypotonic Na chloride, K chloride, Ca chloride and Mg chloride ions through Red Blood Cell (RBC) membrane while the RBCs haemolyse using a UV/Vis spectrophotometer. The effects of the supporting electrolytes were deduced from the changes in their respective rates of permeation through RBC membranes during haemolysis. Na2SO4 added to NaCl and MgSO4 to MgCl2 resulted in a decrease in permeation rate of the resultant solutions while the addition of K2SO4 to KCl and the addition of CaSO4 to CaCl2 resulted in an increase in permeation rate. In this research there was no regular pattern of permeation and since NaSO4 and MgSO4 decreased NaCl and MgCl2 permeability respectively, then the increase in the permeability of KCl and CaCl2 may not be as a result of the increase of K+ and Ca2+ concentration in K2SO4+KCl solution and CaCl2+CaSO4 solution respectively.

Published in Cell Biology (Volume 5, Issue 2)
DOI 10.11648/j.cb.20170502.11
Page(s) 12-16
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Supporting Electrolyte, Permeability, Red Blood Cell, Membrane, Chlorides, Sulphates

References
[1] Okolue, B. N., Ekeocha, C. I. & Ikeaka, B. I. (2014). Study of the effect of pH and supporting electrolytes on the permeation of ions, through a polymeric membrane. Chemical. Society of Nigeria, 39 (2): 40-43.
[2] IUPAC (1997). IUPAC Compendium of Chemical Terminology 2nd edition.
[3] Wang, J. (2006). Analytical Electrochemistry, 3rd edition, Wiley VCH. p. 118.
[4] Yoshida, H., Kawane, K., Koike, M., Mori, Y., Uchiyama, Y. & Nagata, S. (2005). Phosphatidylserine-dependent engulfment by macrophages of nuclei from erythroid precursor cells. Nature, 437 (7059): 754-758
[5] Kundu, M. (2008). Ulk1 plays a critical role in the autophagic clearance of mitochondria and ribosomes during reticulocyte maturation. Blood, 112 (4): 1493-1502.
[6] Mortensen, M., Ferguson, D. J. & Simon, A. K. (2010). Mitochondrial clearance by autophagy in developing erythrocytes: clearly important, but just how much so? Cell Cycle, 9 (10): 1901-1906.
[7] Yawata, Y. (2003). Cell Membrane: The RBC as a Model, Wiley-VCH
[8] Sowemimo-Coker, S. (2002). RBC haemolysis. Transfusion Medicine Review, 16: 146-60
[9] Nepal, O. & Rao, J. P. (2011). Haemolytic effects of hypo-osmotic salt solutions on human erythrocytes. Kathmandu University Medical Journey, 34 (2): 35-39.
[10] Bakko, E. L. (1985). Cell membrane physiology. In Physiology laboratory manual (unpublished). St. Olaf College, Northfield, Minnesota.
[11] Sherwood, L., Klandorf, H. & Yancey, P. B. (2013). Animal Physiology from Genes to Organisms, 2nd edition. Thomson Brooks/Cole, California.
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[13] Ramirez, P., Alcaraz, A., Mafe, S. & Pellicer, J. (1999). pH and supporting electrolyte concentration effects on the passive transport of cationic and anionic drugs through fixed charge membranes. Journal of Membrane Biology, 161: 143-155.
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    Benevolent Orighomisan Atolaiye, Titilayo Oluwayemisi Bamidele, Douglas Uwagbale Edward-Ekpu. (2017). The Effect of Supporting Electrolyte on Ion Permeability Through Red Blood Cell Membrane. Cell Biology, 5(2), 12-16. https://doi.org/10.11648/j.cb.20170502.11

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    ACS Style

    Benevolent Orighomisan Atolaiye; Titilayo Oluwayemisi Bamidele; Douglas Uwagbale Edward-Ekpu. The Effect of Supporting Electrolyte on Ion Permeability Through Red Blood Cell Membrane. Cell Biol. 2017, 5(2), 12-16. doi: 10.11648/j.cb.20170502.11

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    AMA Style

    Benevolent Orighomisan Atolaiye, Titilayo Oluwayemisi Bamidele, Douglas Uwagbale Edward-Ekpu. The Effect of Supporting Electrolyte on Ion Permeability Through Red Blood Cell Membrane. Cell Biol. 2017;5(2):12-16. doi: 10.11648/j.cb.20170502.11

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  • @article{10.11648/j.cb.20170502.11,
      author = {Benevolent Orighomisan Atolaiye and Titilayo Oluwayemisi Bamidele and Douglas Uwagbale Edward-Ekpu},
      title = {The Effect of Supporting Electrolyte on Ion Permeability Through Red Blood Cell Membrane},
      journal = {Cell Biology},
      volume = {5},
      number = {2},
      pages = {12-16},
      doi = {10.11648/j.cb.20170502.11},
      url = {https://doi.org/10.11648/j.cb.20170502.11},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20170502.11},
      abstract = {This study was aimed at providing information on how Na sulphate, K sulphate, Ca sulphate and Mg sulphate supporting electrolytes affect the permeation of hypotonic Na chloride, K chloride, Ca chloride and Mg chloride ions through Red Blood Cell (RBC) membrane while the RBCs haemolyse using a UV/Vis spectrophotometer. The effects of the supporting electrolytes were deduced from the changes in their respective rates of permeation through RBC membranes during haemolysis. Na2SO4 added to NaCl and MgSO4 to MgCl2 resulted in a decrease in permeation rate of the resultant solutions while the addition of K2SO4 to KCl and the addition of CaSO4 to CaCl2 resulted in an increase in permeation rate. In this research there was no regular pattern of permeation and since NaSO4 and MgSO4 decreased NaCl and MgCl2 permeability respectively, then the increase in the permeability of KCl and CaCl2  may not be as a result of the increase of K+ and Ca2+ concentration in K2SO4+KCl solution and CaCl2+CaSO4 solution respectively.},
     year = {2017}
    }
    

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    T1  - The Effect of Supporting Electrolyte on Ion Permeability Through Red Blood Cell Membrane
    AU  - Benevolent Orighomisan Atolaiye
    AU  - Titilayo Oluwayemisi Bamidele
    AU  - Douglas Uwagbale Edward-Ekpu
    Y1  - 2017/03/21
    PY  - 2017
    N1  - https://doi.org/10.11648/j.cb.20170502.11
    DO  - 10.11648/j.cb.20170502.11
    T2  - Cell Biology
    JF  - Cell Biology
    JO  - Cell Biology
    SP  - 12
    EP  - 16
    PB  - Science Publishing Group
    SN  - 2330-0183
    UR  - https://doi.org/10.11648/j.cb.20170502.11
    AB  - This study was aimed at providing information on how Na sulphate, K sulphate, Ca sulphate and Mg sulphate supporting electrolytes affect the permeation of hypotonic Na chloride, K chloride, Ca chloride and Mg chloride ions through Red Blood Cell (RBC) membrane while the RBCs haemolyse using a UV/Vis spectrophotometer. The effects of the supporting electrolytes were deduced from the changes in their respective rates of permeation through RBC membranes during haemolysis. Na2SO4 added to NaCl and MgSO4 to MgCl2 resulted in a decrease in permeation rate of the resultant solutions while the addition of K2SO4 to KCl and the addition of CaSO4 to CaCl2 resulted in an increase in permeation rate. In this research there was no regular pattern of permeation and since NaSO4 and MgSO4 decreased NaCl and MgCl2 permeability respectively, then the increase in the permeability of KCl and CaCl2  may not be as a result of the increase of K+ and Ca2+ concentration in K2SO4+KCl solution and CaCl2+CaSO4 solution respectively.
    VL  - 5
    IS  - 2
    ER  - 

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Author Information
  • Department of Chemistry, Nasarawa State University, Keffi, Nigeria

  • Department of Biochemistry and Molecular Biology, Nasarawa State University, Keffi, Nigeria

  • Department of Chemistry, Nasarawa State University, Keffi, Nigeria

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