Investigation of Immune Biomarkers Regulation by Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Male Sprague Dawley Rats
Cell Biology
Volume 5, Issue 6, November 2017, Pages: 66-75
Received: Oct. 30, 2017; Accepted: Nov. 10, 2017; Published: Dec. 11, 2017
Views 1564      Downloads 89
Authors
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton, Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, Nevada, USA
William Dean Plikerd, Trivedi Global, Inc., Henderson, Nevada, USA
Peter L. Surguy, Trivedi Global, Inc., Henderson, Nevada, USA
Robert John Kock, Trivedi Global, Inc., Henderson, Nevada, USA
Rolando Baptista Piedad, Trivedi Global, Inc., Henderson, Nevada, USA
Russell Phillip Callas, Trivedi Global, Inc., Henderson, Nevada, USA
Sakina A. Ansari, Trivedi Global, Inc., Henderson, Nevada, USA
Sandra Lee Barrett, Trivedi Global, Inc., Henderson, Nevada, USA
Sara Friedman, Trivedi Global, Inc., Henderson, Nevada, USA
Steven Lee Christie, Trivedi Global, Inc., Henderson, Nevada, USA
Su-Mei Chen Liu, Trivedi Global, Inc., Henderson, Nevada, USA
Susan Elizabeth Starling, Trivedi Global, Inc., Henderson, Nevada, USA
Susan Jones, Trivedi Global, Inc., Henderson, Nevada, USA
Susan Mardis Allen, Trivedi Global, Inc., Henderson, Nevada, USA
Susanne Kathrin Wasmus, Trivedi Global, Inc., Henderson, Nevada, USA
Terry Ann Benczik, Trivedi Global, Inc., Henderson, Nevada, USA
Thomas Charles Slade, Trivedi Global, Inc., Henderson, Nevada, USA
Thomas Orban, Trivedi Global, Inc., Henderson, Nevada, USA
Victoria L. Vannes, Trivedi Global, Inc., Henderson, Nevada, USA
Victoria Margot Schlosser, Trivedi Global, Inc., Henderson, Nevada, USA
Yusif Sarkis Yamin Albino, Trivedi Global, Inc., Henderson, Nevada, USA
Mayank Gangwar, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Article Tools
Follow on us
Abstract
Herbomineral formulations have been used worldwide against various chronic and degenerative diseases, due to its fewer side effects. A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract of ashwagandha and minerals (zinc, magnesium, and selenium). The present study aimed to evaluate the impact of Biofield Energy Treated herbomineral formulation in male Sprague Dawley (SD) rats for immune biomarkers modulation. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Treated sample, which received the Biofield Energy Healing Treatment remotely from twenty renowned Biofield Energy Healers. The immunomodulatory biomarkers like alteration in immune biomarkers such as IgG and IgM, CD4+ and CD8+, hematology, lipid profile, hepatic enzymes, sex hormone, and antioxidant profile. The humoral immune response of IgM and IgG were altered by 4.54% and 9.49%, respectively in the Biofield Energy Treated test formulation (G4) group compared with the disease control (G2) group. On the other hand, the cellular immune response i.e. CD4+ and CD8+ counts were significantly increased by 56.21% and 73.6%, respectively in the G4 group compared with the G2. Hematology analysis showed an increased level of TLC and lymphocytes by 14.85% and 21.42%, respectively in the G4 group compared to the G2. Lipid profile analysis showed a significant decrease in the level of total cholesterol, triglycerides, LDL, and VLDL in the G4 group compared with the G2. Further, results suggests a significant decrease in the hepatic biomarkers such as SGOT, SGPT, and cardiac enzyme, CK-MB by 18.54%, 19.12%, and 26.23%, respectively in G4 group compared to the G2 group. However, the testosterone level was significantly decreased in G4 group and untreated test formulation (G5) groups by 53.87% and 26.25%, respectively compared with the G2 group. Additionally, the antioxidant assay data showed an altered level of tested enzymes such as LPO, SOD and CAT, in the G4 group compared with the G2 group. Therefore, it can be concluded that the Biofield Energy Treated test formulation showed a significant improved cellular and humoral immunity, hematological and biochemical profile as compared with the untreated test formulation. As a result, it can be established that The Trivedi Effect®-Biofield Energy Healing has the significant capacity for immunomodulatory effect, which may also be useful in organ transplants, anti-aging, and stress management by improving overall health and quality of life.
Keywords
Biofield Energy Healers, The Trivedi Effect®, Herbomineral Formulation, Autoimmune Diseases, Anti-aging, Testosterone, Anti-oxidation
To cite this article
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, William Dean Plikerd, Peter L. Surguy, Robert John Kock, Rolando Baptista Piedad, Russell Phillip Callas, Sakina A. Ansari, Sandra Lee Barrett, Sara Friedman, Steven Lee Christie, Su-Mei Chen Liu, Susan Elizabeth Starling, Susan Jones, Susan Mardis Allen, Susanne Kathrin Wasmus, Terry Ann Benczik, Thomas Charles Slade, Thomas Orban, Victoria L. Vannes, Victoria Margot Schlosser, Yusif Sarkis Yamin Albino, Mayank Gangwar, Snehasis Jana, Investigation of Immune Biomarkers Regulation by Biofield Energy Healing (The Trivedi Effect®) Based Herbomineral Formulation in Male Sprague Dawley Rats, Cell Biology. Vol. 5, No. 6, 2017, pp. 66-75. doi: 10.11648/j.cb.20170506.12
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
References
[1]
Janeway CA Jr (2001) How the immune system protects the host from infection. Microbes Infect 3: 1167-1171.
[2]
William JE (2001) Review of antiviral and immunomodulatory properties of plants of the Peruvian rainforest. Alter Med Rev 6: 567-579.
[3]
Sharma ML, Rao CS, Duda PL (1994) Immunostimulatory activity of Picrorhiza kurroa leaf extract. J Ethnopharmacol 41: 185-192.
[4]
Davis L, Kuttan G (2000) Immunomodulatory activity of Withania somnifera. J Ethnopharmacol 71: 193-200.
[5]
Lukác N, Massanyi P (2007) Effects of trace elements on the immune system. Epidemiol Mikrobiol Imunol 56: 3-9.
[6]
Wintergerst ES, Maggini S, Hornig DH (2007) Contribution of selected vitamins and trace elements to immune function. Ann Nutr Metab 51: 301‐323.
[7]
Rajagopala S, Ashok BK, Ravishankar B (2011) Immunomodulatory activity of Vachadhatryadi Avaleha in albino rats. Ayu 32: 275-278.
[8]
Meera S, Gupta Atyam VSSS, Kumar NS (2008) Immunomodulatory and antioxidant activity of a polyherbal formulation. Int J Pharm 4: 287-291.
[9]
Farhath S, Vijaya PP, Vima M (2013) Immunomodulatory activity of geranial, geranial acetate, gingerol, and eugenol essential oils: Evidence for humoral and cell-mediated responses. Avicenna J Phytomed 3: 224-230.
[10]
Nelson DS, Mildenhall P (1967) Studies on cytophilic antibodies. The production by mice of macropage cytophilic antibodies to sheep erythrocytes: Relationship to the production of other antibodies and the development of delayed type hypersensitivity. Aust J Exp Biol Med Sci 45: 113-130.
[11]
Mukesh KB, Jitender KJ, Satyanarayana Y, Reddy AD (2012) Animal and plant originate immunostimulants used in aquaculture. Scholars Research Library. J Nat Prod Plant Res 2: 397-400.
[12]
Glotter E, Abraham A, Guenzberg G, Kirson I (1977) Naturally occurring steroidal lactones with 17 α-oriented side chain structure of withanolide E & related compounds. J Chem Soc Perkins Trans 1: 341-346.
[13]
Sohat B, Gitter S, Abraham A, Lavie D (1967) Antitumor activity of withaferin A. Can Chemother Rep 51: 271-276.
[14]
Engle TE, Nockels DF, Kimberling CV, Weaber DL, Johnson AB (1997) Zinc repletion with organic and inorganic forms of zinc and protein turnover in marginally zinc-deficient calves. J Anim Sci 75: 3074-3081.
[15]
Salimian J, Arefpour MA, Riazipour M, Poursasan N (2004) Immunomodulatory effects of selenium and vitamin E on alterations in T lymphocyte subsets induced by T-2 toxin. Immunopharmacol Immunotoxicol 36: 275-281.
[16]
Sangle V, Darp M, Nadkarni S (2004) Evaluation of immunomodulatory activity of Suvarnamalini vasant, a generic Ayurvedic herbomineral formulation. Indian J Exp Biol 42: 115-116.
[17]
Rubik B (1994) Manual healing methods. Alternative medicine: expanding medical horizons, Washington, DC, US Government Printing Office, NIH Publication No. 94-66.
[18]
Cooper EL (2007) The immune system and complementary and alternative medicine. Evid Based Complement Alternat Med 4: 5-8.
[19]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) In vitro evaluation of biofield treatment on viral load against human immunodeficiency-1 and cytomegalo viruses. American Journal of Health Research 3: 338-343.
[20]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) In vitro Evaluation of biofield treatment on Enterobacter cloacae: Impact on antimicrobial susceptibility and biotype. J Bacteriol Parasitol 6: 241.
[21]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) Evaluation of Biofield Modality on Viral Load of Hepatitis B and C Viruses. J Antivir Antiretrovir 7: 83-88.
[22]
Trivedi MK, Patil S, Shettigar H, Mondal SC, Jana S (2015) An impact of biofield treatment: Antimycobacterial susceptibility potential using BACTEC 460/MGIT-TB system. Mycobact Dis 5: 189.
[23]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of antibiogram, genotype and phylogenetic analysis of biofield treated Nocardia otitidis. Biol Syst Open Access 4: 143.
[24]
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2015) Antibiogram, biochemical reactions, and genotypic pattern of biofield treated Pseudomonas aeruginosa. J Trop Dis 4: 181.
[25]
Trivedi MK, Patil S, Tallapragada RM (2013) Effect of bio field treatment on the physical and thermal characteristics of vanadium pentoxide powders. J Material Sci Eng S 11: 001.
[26]
Trivedi MK, Branton A, Trivedi D, Shettigar H, Bairwa K, Jana S (2015) Fourier transform infrared and ultraviolet-visible spectroscopic characterization of biofield treated salicylic acid and sparfloxacin. Nat Prod Chem Res 3: 186.
[27]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Potential impact of biofield treatment on atomic and physical characteristics of magnesium. Vitam Miner 3: 129.
[28]
Trivedi MK, Branton A, Trivedi D, Nayak G, Sethi KK, Jana S (2016) Gas chromatography-mass spectrometry based isotopic abundance ratio analysis of biofield energy treated methyl-2-napthylether (Nerolin). American Journal of Physical Chemistry 5: 80-86.
[29]
Trivedi MK, Branton A, Trivedi D, Nayak G, Panda P, Jana S (2016) Gas chromatography-mass spectrometric analysis of isotopic abundance of 13C, 2H, and 18O in biofield energy treated p-tertiary butylphenol (PTBP). American Journal of Chemical Engineering 4: 78-86.
[30]
Trivedi MK, Branton A, Trivedi D, Nayak G, Mondal SC, Jana S (2015) Evaluation of biochemical marker - glutathione and DNA fingerprinting of biofield energy treated Oryza sativa. American Journal of Bio Science 3: 243-248.
[31]
Trivedi MK, Branton A, Trivedi D, Nayak G, Gangwar M, Jana S (2016) Molecular analysis of biofield treated eggplant and watermelon crops. Adv Crop Sci Tech 4: 208.
[32]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Physical, atomic and thermal properties of biofield treated lithium powder. J Adv Chem Eng 5: 136.
[33]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Latiyal O, Jana S (2015) Evaluation of biofield energy treatment on physical and thermal characteristics of selenium powder. Journal of Food and Nutrition Sciences 3: 223-228.
[34]
Trivedi MK, Tallapragada RM, Branton A, Trivedi D, Nayak G, Mishra RK, Latiyal O, Jana S (2015) Physicochemical characterization of biofield energy treated calcium carbonate powder. American Journal of Health Research 3: 368-375.
[35]
Ladics GS (2007) Primary immune response to sheep red blood cells (SRBC) as the conventional T-cell dependent antibody response (TDAR) test. J Immunotoxicol 4: 149-152.
[36]
King EJ, Armstrong AR (1934) Estimation of alkaline phosphatase. Canad Med Assoc J 311: 152-156.
[37]
Folch J, Lees M, Sloane Stanley GH (1957) A simple method for the isolation and purification of total lipids from animal tissue. J Biol Chem 226: 497-509.
[38]
Devasagayam TPA, Tarachand U (1987) Decreased lipid peroxidation in the rat kidney during gestation. Biochem Biophys Res Commun 145: 134-138.
[39]
Marklund S, Marklund G (1974) Involvement of superoxide anion radical in the autooxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 47: 469-474.
[40]
Sinha AK (1972) Colorimetric assay of catalase. Anal Biochem 47: 389-394.
[41]
Malik F, Singh J, Khajuria A, Suri KA, Satti NK, Singh S, Kaul MK, Kumar A, Bhatia A, Qazi GN (2007) A standardized root extract of Withania somnifera and its major constituent withanolide-A elicit humoral and cell-mediated immune responses by up regulation of Th1-dominant polarization in BALB/c mice. Life Sci 80: 1525-1538.
[42]
Spallholz JE, Stewart JR (1989) Advances in the role of minerals in immunobiology. Biol Trace Elem Res 19: 129-151.
[43]
Uppal SS, Verma S, Dhot PS (2003) Normal values of CD4 and CD8 lymphocyte subsets in healthy indian adults and the effects of sex, age, ethnicity, and smoking. Cytometry B Clin Cytom 52: 32-36.
[44]
Miceli MC, Parnes JR (1991) The roles of CD4 and CD8 in T cell activation. Semin Immunol 3: 133-141.
[45]
Balakrishnan K, Adams LE (1995) The role of the lymphocyte in an immune response. Immunol Invest 24: 233-244.
[46]
Andallu B, Radhika B (2000) Hypoglycemic, diuretic and hypocholesterolemic effect of winter cherry (Withania somnifera, Dunal) root. Indian J Exp Biol 38: 607-609.
[47]
Bunglavan SJ, Garg AK, Dass RS, Shrivastava S (2014) Effect of supplementation of different levels of selenium as nanoparticles/sodium selenite on blood biochemical profile and humoral immunity in male wistar rats. Vet World 7: 1075-1081.
[48]
Fox C, Ramsoomair D, Carter C (2001) Magnesium: its proven and potential clinical significance. South Med J 94: 1195-1201.
[49]
Payahoo L, Ostadrahimi A, Mobasseri M, Bishak YK, Farrin N, Jafarabadi MA, Mahluji S (2013) Effects of zinc supplementation on the anthropometric measurements, lipid profiles and fasting blood glucose in the healthy obese adults. Adv Pharm Bull 3: 161-165.
[50]
Giannini EG, Testa R, Savarino V (2005) Liver enzyme alteration: A guide for clinicians. CMAJ 172: 367-379.
[51]
Sidhu P, Garg ML, Dhawan DK (2005) Protective effects of zinc on oxidative stress enzymes in liver of protein-deficient rats. Drug Chem Toxicol 28: 211-230.
[52]
El-Boshy ME, Risha EF, Abdelhamid FM, Mubarak MS, Hadda TB (2015) Protective effects of selenium against cadmium induced hematological disturbances, immunosuppressive, oxidative stress and hepatorenal damage in rats. J Trace Elem Med Biol 29: 104-110.
[53]
Karandish M, Tamimi M, Shayesteh AA, Haghighizadeh MH, Jalali MT (2013) The effect of magnesium supplementation and weight loss on liver enzymes in patients with nonalcoholic fatty liver disease. J Res Med Sci 18: 573-579.
[54]
Sabiba EP, Rasool M, Vedi M, Navaneethan D, Ravichander M, Parthasarathy P, Thella SR (2013) Hepatoprotective and antioxidant potential of Withania somnifera against paracetamol-induced liver damage in rats. Int J Pharm Pharm Sci 5: 648-651.
[55]
Ambiye VR, Langade D, Dongre S, Aptikar P, Kulkarni M, Dongre A (2013) Clinical Evaluation of the spermatogenic activity of the root extract of ashwagandha (Withania somnifera) in oligospermic males: A pilot study. Evid Based Complement Alternat Med 2013: 571420.
[56]
Shafiei Neek L, Gaeini AA, Choobineh S (2011) Effect of zinc and selenium supplementation on serum testosterone and plasma lactate in cyclist after an exhaustive exercise bout. Biol Trace Elem Res 144: 454-462.
[57]
Cinar V, Polat Y, Baltaci AK, Mogulkoc R (2011) Effects of magnesium supplementation on testosterone levels of athletes and sedentary subjects at rest and after exhaustion. Biol Trace Elem Res 140: 18-23.
[58]
Jana K, Samanta PK, Manna I, Ghosh P, Singh N, Khetan RP, Ray BR (2008) Protective effect of sodium selenite and zinc sulfate on intensive swimming-induced testicular gamatogenic and steroidogenic disorders in mature male rats. Appl Physiol Nutr Metab 33: 903-914.
[59]
Karp SM, Koch TR (2006) Oxidative stress and antioxidants in inflammatory bowel disease. Dis Mon 52: 199-207.
ADDRESS
Science Publishing Group
548 FASHION AVENUE
NEW YORK, NY 10018
U.S.A.
Tel: (001)347-688-8931