Sirtuin Activator and Inhibitor Affect Early <i>Dictyostelium</i> Development upon Starvation

Shuhei Soeda; Hideo Taniura

doi:doi:10.11648/j.cb.20180601.13

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Sirtuin Activator and Inhibitor Affect Early Dictyostelium Development upon Starvation

Shuhei Soeda, Hideo Taniura

Published in Cell Biology (Volume 6, Issue 1)

Received: 3 June 2018 Accepted: 5 July 2018 Published: 2 August 2018

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Abstract

Sirtuin is an evolutionally conserved histone deacetylase. The effects of sirtuin activator, resveratrol and inhibitor, nicotinamide on early Dictyostelium development upon starvation were examined. The cell streaming was appeared at 7 h and tight aggregates was formed at 24 h in control cells under submerged culture. Resveratrol treatment accelerated the development. Cell streaming at 6 h and weak aggregates at 9 h were already observed. While, nicotinamide treatment delayed the development. The initiation of streaming was delayed at 9 h, and the development was still under a cell streaming even after 24 h. Dictyostelium development starts inducing the essential molecules adenylyl cyclase, aca and cAMP pulsing induces cell-surface cAMP receptor (carA). Resveratrol treatment significantly increased the expression level of aca at 4 and 6 h after starvation. Nicotinamide treatment decreased the aca expression level at 4 h and carA expression level at 6 h. The induction of aca upon starvation starts with the transcriptional activation by MybB, a. putative transcription factor. MybB expression recovered the initiation timing of the cell streaming against the effect of nicotinamide, but the aggregates formation was still impaired at 24 h. Resveratrol had no effects on MybB-null mutant cells. Neither streaming nor aggregates formation was observed even after 24 h with low levels of aca expression after starvation by resveratrol treatment. These findings suggest that sirtuins may participate in more than one point of the early developmental processes of Dictyostelium upon starvation.

Published in	Cell Biology (Volume 6, Issue 1)
DOI	10.11648/j.cb.20180601.13
Page(s)	13-19
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Sirtuin, Resveratrol, Nicotinamide, Cellular Development, Dictyostelium discoideum

References

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[6]	H. Otsuka, and P. J. M. Van Haastert. “A novel Myb homolog initiates Dictyostelium development by induction of adenylyl cyclase expression,” 1998, Genes Develop. 12, 1738-1748.
[7]	J. Rodriguez-Centeno, and L. Sastre. “Biological activity of the alternative promoters of the Dictyostelium discoideum adenylyl cyclase A gene,” 2016, PLOS ONE 0148533.
[8]	T. Katayama, and H. Yasukawa. “Analysis of Sir2E in the cellular slime mold Dictyostelium discoideum: Cellular localization, spatial expression and overexpression,” 2008, Develop. Growth. Differ. 50, 645-652.
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APA Style

Shuhei Soeda, Hideo Taniura. (2018). Sirtuin Activator and Inhibitor Affect Early Dictyostelium Development upon Starvation. Cell Biology, 6(1), 13-19. https://doi.org/10.11648/j.cb.20180601.13

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ACS Style

Shuhei Soeda; Hideo Taniura. Sirtuin Activator and Inhibitor Affect Early Dictyostelium Development upon Starvation. Cell Biol. 2018, 6(1), 13-19. doi: 10.11648/j.cb.20180601.13

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AMA Style

Shuhei Soeda, Hideo Taniura. Sirtuin Activator and Inhibitor Affect Early Dictyostelium Development upon Starvation. Cell Biol. 2018;6(1):13-19. doi: 10.11648/j.cb.20180601.13

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@article{10.11648/j.cb.20180601.13,
  author = {Shuhei Soeda and Hideo Taniura},
  title = {Sirtuin Activator and Inhibitor Affect Early Dictyostelium Development upon Starvation},
  journal = {Cell Biology},
  volume = {6},
  number = {1},
  pages = {13-19},
  doi = {10.11648/j.cb.20180601.13},
  url = {https://doi.org/10.11648/j.cb.20180601.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20180601.13},
  abstract = {Sirtuin is an evolutionally conserved histone deacetylase. The effects of sirtuin activator, resveratrol and inhibitor, nicotinamide on early Dictyostelium development upon starvation were examined. The cell streaming was appeared at 7 h and tight aggregates was formed at 24 h in control cells under submerged culture. Resveratrol treatment accelerated the development. Cell streaming at 6 h and weak aggregates at 9 h were already observed. While, nicotinamide treatment delayed the development. The initiation of streaming was delayed at 9 h, and the development was still under a cell streaming even after 24 h. Dictyostelium development starts inducing the essential molecules adenylyl cyclase, aca and cAMP pulsing induces cell-surface cAMP receptor (carA). Resveratrol treatment significantly increased the expression level of aca at 4 and 6 h after starvation. Nicotinamide treatment decreased the aca expression level at 4 h and carA expression level at 6 h. The induction of aca upon starvation starts with the transcriptional activation by MybB, a. putative transcription factor. MybB expression recovered the initiation timing of the cell streaming against the effect of nicotinamide, but the aggregates formation was still impaired at 24 h. Resveratrol had no effects on MybB-null mutant cells. Neither streaming nor aggregates formation was observed even after 24 h with low levels of aca expression after starvation by resveratrol treatment. These findings suggest that sirtuins may participate in more than one point of the early developmental processes of Dictyostelium upon starvation.},
 year = {2018}
}

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TY - JOUR
T1 - Sirtuin Activator and Inhibitor Affect Early Dictyostelium Development upon Starvation
AU - Shuhei Soeda
AU - Hideo Taniura
Y1 - 2018/08/02
PY - 2018
N1 - https://doi.org/10.11648/j.cb.20180601.13
DO - 10.11648/j.cb.20180601.13
T2 - Cell Biology
JF - Cell Biology
JO - Cell Biology
SP - 13
EP - 19
PB - Science Publishing Group
SN - 2330-0183
UR - https://doi.org/10.11648/j.cb.20180601.13
AB - Sirtuin is an evolutionally conserved histone deacetylase. The effects of sirtuin activator, resveratrol and inhibitor, nicotinamide on early Dictyostelium development upon starvation were examined. The cell streaming was appeared at 7 h and tight aggregates was formed at 24 h in control cells under submerged culture. Resveratrol treatment accelerated the development. Cell streaming at 6 h and weak aggregates at 9 h were already observed. While, nicotinamide treatment delayed the development. The initiation of streaming was delayed at 9 h, and the development was still under a cell streaming even after 24 h. Dictyostelium development starts inducing the essential molecules adenylyl cyclase, aca and cAMP pulsing induces cell-surface cAMP receptor (carA). Resveratrol treatment significantly increased the expression level of aca at 4 and 6 h after starvation. Nicotinamide treatment decreased the aca expression level at 4 h and carA expression level at 6 h. The induction of aca upon starvation starts with the transcriptional activation by MybB, a. putative transcription factor. MybB expression recovered the initiation timing of the cell streaming against the effect of nicotinamide, but the aggregates formation was still impaired at 24 h. Resveratrol had no effects on MybB-null mutant cells. Neither streaming nor aggregates formation was observed even after 24 h with low levels of aca expression after starvation by resveratrol treatment. These findings suggest that sirtuins may participate in more than one point of the early developmental processes of Dictyostelium upon starvation.
VL - 6
IS - 1
ER -

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Author Information

Shuhei Soeda

Laboratory of Neurochemistry, College of Pharmaceutical Sciences, Ritsumeikan University, Shiga, Japan
Hideo Taniura

Laboratory of Neurochemistry, College of Pharmaceutical Sciences, Ritsumeikan University, Shiga, Japan

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APA Style

Shuhei Soeda, Hideo Taniura. (2018). Sirtuin Activator and Inhibitor Affect Early Dictyostelium Development upon Starvation. Cell Biology, 6(1), 13-19. https://doi.org/10.11648/j.cb.20180601.13

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ACS Style

Shuhei Soeda; Hideo Taniura. Sirtuin Activator and Inhibitor Affect Early Dictyostelium Development upon Starvation. Cell Biol. 2018, 6(1), 13-19. doi: 10.11648/j.cb.20180601.13

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AMA Style

Shuhei Soeda, Hideo Taniura. Sirtuin Activator and Inhibitor Affect Early Dictyostelium Development upon Starvation. Cell Biol. 2018;6(1):13-19. doi: 10.11648/j.cb.20180601.13

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@article{10.11648/j.cb.20180601.13,
  author = {Shuhei Soeda and Hideo Taniura},
  title = {Sirtuin Activator and Inhibitor Affect Early Dictyostelium Development upon Starvation},
  journal = {Cell Biology},
  volume = {6},
  number = {1},
  pages = {13-19},
  doi = {10.11648/j.cb.20180601.13},
  url = {https://doi.org/10.11648/j.cb.20180601.13},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20180601.13},
  abstract = {Sirtuin is an evolutionally conserved histone deacetylase. The effects of sirtuin activator, resveratrol and inhibitor, nicotinamide on early Dictyostelium development upon starvation were examined. The cell streaming was appeared at 7 h and tight aggregates was formed at 24 h in control cells under submerged culture. Resveratrol treatment accelerated the development. Cell streaming at 6 h and weak aggregates at 9 h were already observed. While, nicotinamide treatment delayed the development. The initiation of streaming was delayed at 9 h, and the development was still under a cell streaming even after 24 h. Dictyostelium development starts inducing the essential molecules adenylyl cyclase, aca and cAMP pulsing induces cell-surface cAMP receptor (carA). Resveratrol treatment significantly increased the expression level of aca at 4 and 6 h after starvation. Nicotinamide treatment decreased the aca expression level at 4 h and carA expression level at 6 h. The induction of aca upon starvation starts with the transcriptional activation by MybB, a. putative transcription factor. MybB expression recovered the initiation timing of the cell streaming against the effect of nicotinamide, but the aggregates formation was still impaired at 24 h. Resveratrol had no effects on MybB-null mutant cells. Neither streaming nor aggregates formation was observed even after 24 h with low levels of aca expression after starvation by resveratrol treatment. These findings suggest that sirtuins may participate in more than one point of the early developmental processes of Dictyostelium upon starvation.},
 year = {2018}
}

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