Cell culture is an important tool in medical, odontological and biological research laboratories, supporting cell therapies and tissue bioengineering strategies. It is used as a means for in vitro testing of the biocompatibility of resin polymers used in dentistry. The majority of cells are cultured with Dulbecco's modified Eagle's medium (DMEM) or RPMI supplemented with fetal bovine serum. Several cell types are being studied including gingival fibroblasts. Gingival fibroblasts are the main cells of gingival connective tissue. These cells play an active and important role in almost all coating fabric processes, and its involvement in various pathophysiological conditions, including, healing, repair, aging, psoriasis, cancer among others, is only beginning to be understood. DMEM is the most widely used fibroblastic culture medium. This model describes a method for obtaining and cultivating human gingival fibroblasts, by explants derived from surgical discards. Fibroblasts were isolated mechanically and cultured in RPMI 1640 culture medium supplemented with fetal bovine serum 10%, Penicillin (10000 U/ml)/Streptomycin (10 mg/ml) 1% and L-Glutamine (200 mM) 1%. The culture medium is replaced every two days. Cells forming a fairly dense network were observed after a period of 4 days of culture. Human gingival fibroblasts can be cultured by direct explant technique with RPMI 1640 culture medium supplemented with fetal bovine serum and antibiotics.
| DOI | 10.11648/j.cb.20200801.12 |
| Published in | Cell Biology (Volume 8, Issue 1, June 2020) |
| Page(s) | 8-11 |
| Creative Commons |
This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited. |
| Copyright |
Copyright © The Author(s), 2024. Published by Science Publishing Group |
Cell Culture, Gingival Fibroblast, RPMI 1640 Culture Medium
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APA Style
Raoul Bationo, Ablassé Rouamba, Abdoulaziz Diarra, Monique Lydie Beugré-Kouassi, Fabienne Jordana, et al. (2020). Culture of Human Gingival Fibroblasts: An Experimental Model. Cell Biology, 8(1), 8-11. https://doi.org/10.11648/j.cb.20200801.12
ACS Style
Raoul Bationo; Ablassé Rouamba; Abdoulaziz Diarra; Monique Lydie Beugré-Kouassi; Fabienne Jordana, et al. Culture of Human Gingival Fibroblasts: An Experimental Model. Cell Biol. 2020, 8(1), 8-11. doi: 10.11648/j.cb.20200801.12
AMA Style
Raoul Bationo, Ablassé Rouamba, Abdoulaziz Diarra, Monique Lydie Beugré-Kouassi, Fabienne Jordana, et al. Culture of Human Gingival Fibroblasts: An Experimental Model. Cell Biol. 2020;8(1):8-11. doi: 10.11648/j.cb.20200801.12
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Download@article{10.11648/j.cb.20200801.12,
author = {Raoul Bationo and Ablassé Rouamba and Abdoulaziz Diarra and Monique Lydie Beugré-Kouassi and Fabienne Jordana and Jean-Bertin Beugré},
title = {Culture of Human Gingival Fibroblasts: An Experimental Model},
journal = {Cell Biology},
volume = {8},
number = {1},
pages = {8-11},
doi = {10.11648/j.cb.20200801.12},
url = {https://doi.org/10.11648/j.cb.20200801.12},
eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20200801.12},
abstract = {Cell culture is an important tool in medical, odontological and biological research laboratories, supporting cell therapies and tissue bioengineering strategies. It is used as a means for in vitro testing of the biocompatibility of resin polymers used in dentistry. The majority of cells are cultured with Dulbecco's modified Eagle's medium (DMEM) or RPMI supplemented with fetal bovine serum. Several cell types are being studied including gingival fibroblasts. Gingival fibroblasts are the main cells of gingival connective tissue. These cells play an active and important role in almost all coating fabric processes, and its involvement in various pathophysiological conditions, including, healing, repair, aging, psoriasis, cancer among others, is only beginning to be understood. DMEM is the most widely used fibroblastic culture medium. This model describes a method for obtaining and cultivating human gingival fibroblasts, by explants derived from surgical discards. Fibroblasts were isolated mechanically and cultured in RPMI 1640 culture medium supplemented with fetal bovine serum 10%, Penicillin (10000 U/ml)/Streptomycin (10 mg/ml) 1% and L-Glutamine (200 mM) 1%. The culture medium is replaced every two days. Cells forming a fairly dense network were observed after a period of 4 days of culture. Human gingival fibroblasts can be cultured by direct explant technique with RPMI 1640 culture medium supplemented with fetal bovine serum and antibiotics.},
year = {2020}
}
TY - JOUR T1 - Culture of Human Gingival Fibroblasts: An Experimental Model AU - Raoul Bationo AU - Ablassé Rouamba AU - Abdoulaziz Diarra AU - Monique Lydie Beugré-Kouassi AU - Fabienne Jordana AU - Jean-Bertin Beugré Y1 - 2020/06/20 PY - 2020 N1 - https://doi.org/10.11648/j.cb.20200801.12 DO - 10.11648/j.cb.20200801.12 T2 - Cell Biology JF - Cell Biology JO - Cell Biology SP - 8 EP - 11 PB - Science Publishing Group SN - 2330-0183 UR - https://doi.org/10.11648/j.cb.20200801.12 AB - Cell culture is an important tool in medical, odontological and biological research laboratories, supporting cell therapies and tissue bioengineering strategies. It is used as a means for in vitro testing of the biocompatibility of resin polymers used in dentistry. The majority of cells are cultured with Dulbecco's modified Eagle's medium (DMEM) or RPMI supplemented with fetal bovine serum. Several cell types are being studied including gingival fibroblasts. Gingival fibroblasts are the main cells of gingival connective tissue. These cells play an active and important role in almost all coating fabric processes, and its involvement in various pathophysiological conditions, including, healing, repair, aging, psoriasis, cancer among others, is only beginning to be understood. DMEM is the most widely used fibroblastic culture medium. This model describes a method for obtaining and cultivating human gingival fibroblasts, by explants derived from surgical discards. Fibroblasts were isolated mechanically and cultured in RPMI 1640 culture medium supplemented with fetal bovine serum 10%, Penicillin (10000 U/ml)/Streptomycin (10 mg/ml) 1% and L-Glutamine (200 mM) 1%. The culture medium is replaced every two days. Cells forming a fairly dense network were observed after a period of 4 days of culture. Human gingival fibroblasts can be cultured by direct explant technique with RPMI 1640 culture medium supplemented with fetal bovine serum and antibiotics. VL - 8 IS - 1 ER -
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