Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells

Jian Tu; Ting Xiong; Shujing Teng; Zhigang Zhou; Xiaoyong Lei; Shengsong Tang

doi:doi:10.11648/j.cb.20170501.11

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Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells

Jian Tu, Ting Xiong, Shujing Teng, Zhigang Zhou, Xiaoyong Lei, Shengsong Tang

Published in Cell Biology (Volume 5, Issue 1)

Received: 31 January 2017 Accepted: 20 February 2017 Published: 4 March 2017

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Abstract

Macrophage colony-stimulating factor (M-CSF), also named colony-stimulating factor-1 (CSF-1), plays an important role in the process of proliferation and differentiation of the monocyte/macrophage lineage cells. Commonly, it is not easy to measure the expression of cellular M-CSF. However, recent studies have shown that M-CSF can be expressed at a high level in the cytoplasm and nuclei of some kinds of malignant tumors, which related to the poor prognosis. To explore the role and mechanism of nuclear M-CSF, in the present study we constructed the pCMV/nuc/M-CSF vector and transfected it into human HeLa nuclei. The results from our previous study indicated, M-CSF was stably expressed in HeLa nuclei, which were used as a model to determine the nuclear effects of M-CSF. There was a higher percentage of replicating nuclei in the transfected pCMV/nuc/M-CSF HeLa cells both in phase G1 and S. According to the data from the cell doubling time, antisense oligonucleotides and the experiments of the transplanted tumor in nude mice, nuclear M-CSF could promote the cell proliferation of HeLa cells both in vivo and in vitro. In conclusion, nuclear M-CSF could accelerate DNA replication and cell proliferation of cervical carcinoma.

Published in	Cell Biology (Volume 5, Issue 1)
DOI	10.11648/j.cb.20170501.11
Page(s)	1-7
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Microphage Colony-Stimulating Factor (M-CSF), HeLa Nuclei, DNA Replication, Cell Proliferation

References

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APA Style

Jian Tu, Ting Xiong, Shujing Teng, Zhigang Zhou, Xiaoyong Lei, et al. (2017). Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells. Cell Biology, 5(1), 1-7. https://doi.org/10.11648/j.cb.20170501.11

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ACS Style

Jian Tu; Ting Xiong; Shujing Teng; Zhigang Zhou; Xiaoyong Lei, et al. Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells. Cell Biol. 2017, 5(1), 1-7. doi: 10.11648/j.cb.20170501.11

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AMA Style

Jian Tu, Ting Xiong, Shujing Teng, Zhigang Zhou, Xiaoyong Lei, et al. Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells. Cell Biol. 2017;5(1):1-7. doi: 10.11648/j.cb.20170501.11

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@article{10.11648/j.cb.20170501.11,
  author = {Jian Tu and Ting Xiong and Shujing Teng and Zhigang Zhou and Xiaoyong Lei and Shengsong Tang},
  title = {Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells},
  journal = {Cell Biology},
  volume = {5},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.cb.20170501.11},
  url = {https://doi.org/10.11648/j.cb.20170501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20170501.11},
  abstract = {Macrophage colony-stimulating factor (M-CSF), also named colony-stimulating factor-1 (CSF-1), plays an important role in the process of proliferation and differentiation of the monocyte/macrophage lineage cells. Commonly, it is not easy to measure the expression of cellular M-CSF. However, recent studies have shown that M-CSF can be expressed at a high level in the cytoplasm and nuclei of some kinds of malignant tumors, which related to the poor prognosis. To explore the role and mechanism of nuclear M-CSF, in the present study we constructed the pCMV/nuc/M-CSF vector and transfected it into human HeLa nuclei. The results from our previous study indicated, M-CSF was stably expressed in HeLa nuclei, which were used as a model to determine the nuclear effects of M-CSF. There was a higher percentage of replicating nuclei in the transfected pCMV/nuc/M-CSF HeLa cells both in phase G1 and S. According to the data from the cell doubling time, antisense oligonucleotides and the experiments of the transplanted tumor in nude mice, nuclear M-CSF could promote the cell proliferation of HeLa cells both in vivo and in vitro. In conclusion, nuclear M-CSF could accelerate DNA replication and cell proliferation of cervical carcinoma.},
 year = {2017}
}

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TY  - JOUR
T1  - Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells
AU  - Jian Tu
AU  - Ting Xiong
AU  - Shujing Teng
AU  - Zhigang Zhou
AU  - Xiaoyong Lei
AU  - Shengsong Tang
Y1  - 2017/03/04
PY  - 2017
N1  - https://doi.org/10.11648/j.cb.20170501.11
DO  - 10.11648/j.cb.20170501.11
T2  - Cell Biology
JF  - Cell Biology
JO  - Cell Biology
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2330-0183
UR  - https://doi.org/10.11648/j.cb.20170501.11
AB  - Macrophage colony-stimulating factor (M-CSF), also named colony-stimulating factor-1 (CSF-1), plays an important role in the process of proliferation and differentiation of the monocyte/macrophage lineage cells. Commonly, it is not easy to measure the expression of cellular M-CSF. However, recent studies have shown that M-CSF can be expressed at a high level in the cytoplasm and nuclei of some kinds of malignant tumors, which related to the poor prognosis. To explore the role and mechanism of nuclear M-CSF, in the present study we constructed the pCMV/nuc/M-CSF vector and transfected it into human HeLa nuclei. The results from our previous study indicated, M-CSF was stably expressed in HeLa nuclei, which were used as a model to determine the nuclear effects of M-CSF. There was a higher percentage of replicating nuclei in the transfected pCMV/nuc/M-CSF HeLa cells both in phase G1 and S. According to the data from the cell doubling time, antisense oligonucleotides and the experiments of the transplanted tumor in nude mice, nuclear M-CSF could promote the cell proliferation of HeLa cells both in vivo and in vitro. In conclusion, nuclear M-CSF could accelerate DNA replication and cell proliferation of cervical carcinoma.
VL  - 5
IS  - 1
ER  -

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Author Information

Jian Tu

Department of Pharmacology, University of South China, Hengyang, China
Ting Xiong

Department of Pharmacology, University of South China, Hengyang, China
Shujing Teng

Department of Pharmacology, University of South China, Hengyang, China
Zhigang Zhou

Department of Anesthesiology, University of South China, Hengyang, China
Xiaoyong Lei

Department of Pharmacology, University of South China, Hengyang, China
Shengsong Tang

Department of Pharmacology, University of South China, Hengyang, China

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Plain Text BibTeX RIS

APA Style

Jian Tu, Ting Xiong, Shujing Teng, Zhigang Zhou, Xiaoyong Lei, et al. (2017). Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells. Cell Biology, 5(1), 1-7. https://doi.org/10.11648/j.cb.20170501.11

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ACS Style

Jian Tu; Ting Xiong; Shujing Teng; Zhigang Zhou; Xiaoyong Lei, et al. Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells. Cell Biol. 2017, 5(1), 1-7. doi: 10.11648/j.cb.20170501.11

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AMA Style

Jian Tu, Ting Xiong, Shujing Teng, Zhigang Zhou, Xiaoyong Lei, et al. Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells. Cell Biol. 2017;5(1):1-7. doi: 10.11648/j.cb.20170501.11

Copy | Download

@article{10.11648/j.cb.20170501.11,
  author = {Jian Tu and Ting Xiong and Shujing Teng and Zhigang Zhou and Xiaoyong Lei and Shengsong Tang},
  title = {Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells},
  journal = {Cell Biology},
  volume = {5},
  number = {1},
  pages = {1-7},
  doi = {10.11648/j.cb.20170501.11},
  url = {https://doi.org/10.11648/j.cb.20170501.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cb.20170501.11},
  abstract = {Macrophage colony-stimulating factor (M-CSF), also named colony-stimulating factor-1 (CSF-1), plays an important role in the process of proliferation and differentiation of the monocyte/macrophage lineage cells. Commonly, it is not easy to measure the expression of cellular M-CSF. However, recent studies have shown that M-CSF can be expressed at a high level in the cytoplasm and nuclei of some kinds of malignant tumors, which related to the poor prognosis. To explore the role and mechanism of nuclear M-CSF, in the present study we constructed the pCMV/nuc/M-CSF vector and transfected it into human HeLa nuclei. The results from our previous study indicated, M-CSF was stably expressed in HeLa nuclei, which were used as a model to determine the nuclear effects of M-CSF. There was a higher percentage of replicating nuclei in the transfected pCMV/nuc/M-CSF HeLa cells both in phase G1 and S. According to the data from the cell doubling time, antisense oligonucleotides and the experiments of the transplanted tumor in nude mice, nuclear M-CSF could promote the cell proliferation of HeLa cells both in vivo and in vitro. In conclusion, nuclear M-CSF could accelerate DNA replication and cell proliferation of cervical carcinoma.},
 year = {2017}
}

Copy | Download

TY  - JOUR
T1  - Nuclear M-CSF Accelerates DNA Replication and Cell Proliferation in HeLa Human Cervical Cancer Cells
AU  - Jian Tu
AU  - Ting Xiong
AU  - Shujing Teng
AU  - Zhigang Zhou
AU  - Xiaoyong Lei
AU  - Shengsong Tang
Y1  - 2017/03/04
PY  - 2017
N1  - https://doi.org/10.11648/j.cb.20170501.11
DO  - 10.11648/j.cb.20170501.11
T2  - Cell Biology
JF  - Cell Biology
JO  - Cell Biology
SP  - 1
EP  - 7
PB  - Science Publishing Group
SN  - 2330-0183
UR  - https://doi.org/10.11648/j.cb.20170501.11
AB  - Macrophage colony-stimulating factor (M-CSF), also named colony-stimulating factor-1 (CSF-1), plays an important role in the process of proliferation and differentiation of the monocyte/macrophage lineage cells. Commonly, it is not easy to measure the expression of cellular M-CSF. However, recent studies have shown that M-CSF can be expressed at a high level in the cytoplasm and nuclei of some kinds of malignant tumors, which related to the poor prognosis. To explore the role and mechanism of nuclear M-CSF, in the present study we constructed the pCMV/nuc/M-CSF vector and transfected it into human HeLa nuclei. The results from our previous study indicated, M-CSF was stably expressed in HeLa nuclei, which were used as a model to determine the nuclear effects of M-CSF. There was a higher percentage of replicating nuclei in the transfected pCMV/nuc/M-CSF HeLa cells both in phase G1 and S. According to the data from the cell doubling time, antisense oligonucleotides and the experiments of the transplanted tumor in nude mice, nuclear M-CSF could promote the cell proliferation of HeLa cells both in vivo and in vitro. In conclusion, nuclear M-CSF could accelerate DNA replication and cell proliferation of cervical carcinoma.
VL  - 5
IS  - 1
ER  -

Copy | Download