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Cellular Interactions, Metabolism, Assessment and Control of Aflatoxins: An Update

Received: 22 June 2020    Accepted: 2 November 2020    Published: 19 November 2020
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Abstract

Aspergillus spp., the fungus containing aflatoxin, is commonly spread in nature and contains severely polluted food sources from humans and wildlife, resulting in risks to health and even mortality of species. In plants, such as maize and peanuts, the spores of Aspergillus paraciticus and Aspergillus flavus can grow on the surface of stigma. The germ tube goes into the developing embryo and mimics pollen germ tubes. Aflatoxins are naturally occurring substances, so it is difficult to remove them completely from products. However, they should be lessened to minimum possible level. There is also a strong need for research into aflatoxins to establish effective methods for their correct identification , quantification and monitoring to ensure public health safety. The chemistry and biosynthesis process of aflatoxins is addressed in a succinct fashion along with their occurrence and the toxic health threats to humans and livestock. This analysis focuses primarily on aflatoxin tools, development, identification and control techniques to ensure food and feed safety. The study is very useful to health-conscious customers and academic authorities in the related fields. In addition , the availability of information on toxicity of aflatoxins would help ensure food safety and solve potential problems for the rising population by reducing the occurrence of outbreaks related to aflatoxins.

Published in Computational Biology and Bioinformatics (Volume 8, Issue 2)
DOI 10.11648/j.cbb.20200802.15
Page(s) 62-71
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Aflatoxins, Aspergillus Sp., Secondary Metabolites, Food Contamination, Detoxification

References
[1] Abyaneh, M. R., Ghahfarokhi M. S, Yoshinari T., Rezaee M. B, Jaimand K., Nagasawa H., Sakuda, S., (2008). Inhibitory effects of Satureja hortensis L. essential oil on growth and aflatoxin production by Aspergillus parasiticus. International Journal of Food Microbiology. 123 (3), 228-233.
[2] Adebayo-Tayo, B. C., Onilude A. A., Patrick, U. G., (2008). Mycofloral of smoke-dried fishes sold in Uyo, Eastern Nigeria. World Journal of Agricultural Sciences. 4 (3), 346-350.
[3] Albores, A. M., Garcia J. C. D. R., Martinez, E. M., (2007). Decontamination of aflatoxin duckling feed with aqueous citric acid treatment. Animal Feed Science and Technology. 135, 249-262.
[4] Ambrose, E. O., Yao H., Hruska, Z., Kincaid, R., Brown, R. L., Cleveland, T. E., (2009). Automatic detection of aflatoxin contaminated corn kernels using dual-band imagery. Proceedings of SPIE. 7315, 1-11.
[5] Anokwuru, C. P., Ezekiel, C. N. Alabi, O. A., Shallie P. D., Haruna, M. T., (2011). Ameliorative effects of ethanolic neem extract on dietary aflatoxin induced hematological damage and hepatotoxicity in mice. Asian Journal of Medical Sciences. 3 (6), 254-260.
[6] Bbosa, G. S., Kitya, D., Odda, J., Okeng, J. O., (2013). Aflatoxins metabolism, effects on epigenetic mechanisms and their role in carcinogenesis. Health. 5 (10), 14-34.
[7] Reid, C. X. Sparks, D. L., Williams, W. P., Brown A. E. (2016). Single Corn Kernel Aflatoxin B1 Extraction and Analysis Method. Natural Resources. 7, 405-410.
[8] Castell, J. V., Donato M. T., Lechon, M. J. G., (2005). Metabolism and bioactivation of toxicants in the lung. The in vitro cellular approach. Experimental and Toxicologic Pathology. 57, 189-204.
[9] Cavin, C., Mace, K., Offord, E. A., Schilter, B., (2001). Protective effects of coffee dipertenes against aflatoxin B1- induced genotoxicity: mechanism in rats and human cells. Food and Chemical Toxicology. 39, 549-556.
[10] Chen, C. Y., Li W. J., Peng, K. Y., (2005). Determination of Aflatoxin M1 in milk and milk powder using high-flow solid-phase extraction and liquid chromatography tandem mass spectrometry. Journal of Agricultural and Food Chemistry. 53, 8474-8480.
[11] Choi, K. C., Lee, B. S., Chung, W. T., Choi, M. S., Lee, J. C., (2010). Protective effects of apigenin and quercitin on aflatoxin B1-induced immunotoxicity in mice. Food Science and Biotechnology. 19 (4), 987-992.
[12] Colak, H., Bingol, E. B., Hampikyan, H., Nazli, B., (2006). Determination of aflatoxin contamination in red-scaled, red and black pepper by ELISA and HPLC. Journal of Food and Drug Analysis. 14 (3), 292-296.
[13] Cusato S., Gameiro A. H., Corassin C. H., Sant’Ana A. S., Cruz A. G., Faria J. D. A. F., (2013). Food safety systems in a small dairy factory: implementation, major challenges, and assessment of systems’ performances. Foodborne Pathog. Dis. 10: 6–12.
[14] D. O. Okun, Khamis, F. M., Muluvi, G. M., Ngeranwa, J. J., Ombura, F. O., Yongo, M. O., Kenya, E. U., (2015). Distribution of indigenous strains of atoxigenic and toxigenic Aspergillus flavus and Aspergillus parasiticus in maize and peanuts agro-ecological zones of Kenya. Agriculture and Food Security. 4 (14), 1-10.
[15] Dashwood, R., Negishi, T. Hayatsu, H., Breinholt, V., Hendricks, J., Bailey, G., (1998). Chemopreventive properties of chlorophylls towards aflatoxin B1: a review of the antimutagenicity and anticarcinogenicity data in rainbow trout. Mutation Research. 399, 245-253.
[16] Edrington, T. S., Sarrb, A. B., Kubenaa, L. F., Harvey, R. B., Phillips, T. D., (1996). Hydrated sodium calcium aluminosilicate (HSCAS), acidic HSCAS, and activated charcoal reduce urinary excretion of aflatoxin M1, in turkey poults. Lack of effect by activated charcoal on aflatoxicosis. Toxicology Letters. 89, 115-122.
[17] Egal, S., Hounsa, A., Gong, Y. Y., Turner, P. C., Wild, C. P., Hall, A. J., Hell, K., Cardwell, K. F., (2005). Dietary exposure to aflatoxin from maize and groundnut in young children from Benin and Togo, West Africa. International Journal of Food Microbiology. 104, 215-224.
[18] Forouharmehr, A., Harkinezhad, T., Panahi, B. Q., (2013). Effect of aflatoxin B1 on growth of bovine mammary epithelial cells in 3D and monolayer culture system. Advanced Pharmaceutical Bulletin. 3 (1), 143-146.
[19] Galtier, P., Meissonnier, G., Laffitte, J., Oswald, I. P., Loiseau, N., (2008). Molecular interactions between mycotoxins and liver enzymes involved in drug metabolism in rodents and farm animals. Krmiva. 4, 205-213.
[20] Ghosh, M. K., Chhabra, A., Atreja, P. P., Chopra, R. C., (1996). Effect of treating with propionic acid, sodium bisulfite and sodium hydroxide on the biosynthesis of aflatoxin on groundnut cake. Animal Feed Science Technology. 60, 43-49.
[21] Gouas, D., Shi, H., Hainaut, P., (2009). The aflatoxin-induced TP53 mutation at codon 249 (R249S): Biomarker of exposure, early detection and target for therapy. Cancer Letters. 286, 29-37.
[22] Gowda, N. K. S., Malathi, V. Suganthi, R. U., (2004). Effect of some chemical and herbal compounds on growth of Aspergillus parasiticus and aflatoxin production. Animal Feed Science and Technology. 116 (3), 281-291.
[23] Guengerich, F. P., Johnson, W. W., Ueng, Y. F., Yamazaki, H., Shimada, T., (1996). Involvement of cytochrome P450, glutathione S-transferase, and epoxide hydrolase in the metabolism of aflatoxin B1 and relevance to risk of human liver cancer. Enviromental Health Perspectives. 104 (3), 557-562.
[24] Hassan, S M, Sultana B, Iqbal M, Naz S, Abbas M (2017). Anti-aflatoxigenic activity of Punica granatum and Ziziphus jujuba leaves against Aspergillus parasiticus inoculated poultry feed: Effect of storage conditions. Biocatalysis and Agricultural Biotechnology. 10: 104-112.
[25] Hiruma, S., Kimura, M., Lehmanna, K., Gopalan-Kriczkya, P., Qin, G., Shinozukab, H., Sate, K., Lotlikara, P. D., (1997). Potentiation of aflatoxin B1-induced hepatocarcinogenesis in the rat by pretreatment with buthionine sulfoximine. Cancer Letters. 113, 103-109.
[26] Holcomb, H., Wilson, D. M., Trucksess, M. W., Thompson, H. C., (1992). Determination of aflatoxins in food products by chromatography. Journal of Chromatography. 624, 341-352.
[27] Hussain I., Anwar J., (2008). A study on contamination of aflatoxin M1 in raw milk in the Punjab province of Pakistan. Food Control. 19, 393–395.
[28] Hussain, I., Anwar, J., Munawar, M. A., Asi, M. R. (2008). Variation of levels of aflatoxin M1 in raw milk from different localities in the central areas of Punjab, Pakistan. Food Control. 19 (12): 1126-1129.
[29] Yu. J., (2012). Current Understanding on Aflatoxin Biosynthesis and FuturePerspective in Reducing Aflatoxin Contamination. Toxins. 4, 1024-1057
[30] Khatun, S., Chakraborty, M., Islam, A., Cakilcioglu, U., Chatterjee, N. C., (2012). Mycotoxins as health hazard. Biological Diversity and Conservation. 5 (3), 123-133.
[31] Kumar, A., P. Ponnan, H. G. Raj, V. S. Parmar and L. Saso. (2013). Comparative specificities of calreticulin transacetylase to O-acetyl, N-acetyl and S-acetyl derivative of 4-methylcoumarins and their inhibitory effect on AFB1-induced genotoxicity in vitro and in vivo. Food and Chemical Toxicology. 52: 216-224.
[32] Langeswaran, K., R. Revathy, S. G. Kumar, S. Vijayaprakash and M. P. Balasubramanian. (2012). Kaempferol ameliorates aflatoxin B1 (AFB1) induced hepatocellular carcinoma through modifying metabolizing enzymes, membrane bound ATPases and mitochondrial TCA cycle enzymes. Asian Pacific Journal of Tropical Biomedicine. 1653-1659.
[33] Mahanti N., Bhatnagar D., Cary J. W., Joubran J., Linz J. E. (1996). Structure and function of fas-1A, a gene encoding a putative fatty acid synthetase directly involved in aflatoxin biosynthesis in Aspergillus parasiticus. Appl. Environ. Microbiol. 62 191–195
[34] Martins, H. M., M. M. M. Guerra and F. M. A. Bernardo. (2007). Occurrence of aflatoxin B1 in dairy cow’s feed over 10 years in Portugal (1995-2004). Revista Iberoamericana De Micologia. 24: 69-71.
[35] Martins, M., A. M. Kluczkovski, T. P. D. Souza, C. Pacheco, G. D. Savi and V. M. Scussel. (2014). Inhibition of growth and aflatoxin production of Aspergillus parasiticus by guarana (Paullinia cupana) and juca (Libidibia ferrea) extracts. African Journal of Biotechnology. 13 (1): 131-137.
[36] Mathuria, N. and R. J. Y. Verma. (2007). Ameliorative effect of curcumin on aflatoxin-induced toxicity in DNA, RNA and protein in liver and kidney of mice. Acta Poloniae Pharmaceutica Drug Research. 64 (6): 497-502.
[37] Mclean, M. and M. F. Dutton. (1995). Cellular interactions and metabolism of aflatoxins: an update. Pharmacology and Therapeutics. 65: 163-192.
[38] Moss, M. O. (2002). Risk assessment for aflatoxins in foodstuffs. International Biodeterioration and Biodegradation. 50: 137-142.
[39] Muller, P. and T. Basedow. (2007). Aflatoxin contamination of pods of Indian Cassia senna L. (Caesalpinaceae) before harvest, during drying and in storage: Reasons and possible methods of reduction. Journal of Stored Products Research. 43: 323-329.
[40] Mushtaq, M., B. Sultana, F. Anwar, M. Z. Khan and M. Ashrafuzzaman (2012). Occurance of aflatoxins in selected processed foods from Pakistan. International Journal of Molecular Sciences. 13. 8324-8337.
[41] Natale, F. D., M. Gallo and R. Nigro (2009). Adsorbents selection for aflatoxins removal in bovine milks. Journal of Food Engineering. 95: 186-191.
[42] Ozaslan, M., D. Caliskan, I. H. Killic and I. D. Karagoz. (2011). Application of the ELISA and HPLC test for detection of aflatoxin in Pistachio. Scientific Research and Essays. 6 (14): 2913-2917.
[43] Ozdemir, M. (2007). Determination of aflatoxin M1 levels in goat milk consumed in Kilis province. Ankara Universitesi Veteriner Fakultesi Dergisi. 54: 99-103.
[44] P. Kumar, D. K. Mahato, M. Kamle, T. K. Mohanta and S. G. Kang. (2017). Aflatoxins: A Global Concern for Food Safety, Human Health and Their Management. Frontiers in microbiology. 7: doi: 10.3389/fmicb.2016.02170.
[45] P. Udomkun, A. N. Wiredub, J. Müllerc, B. Vanlauwed, R. Bandyopadhyay. (2017). Innovative technologies to manage aflatoxins in foods and feeds and the profitability of application – A review. Food control. 76: 127-138.
[46] Prandini, A., G. Tansini, S. Sigolo, L. Filippi, M. Laporta and G. Piva. (2009). On the occurrence of aflatoxin M1 in milk and dairy products. Food and Chemical Toxicology. 47: 984-991.
[47] Quist, C. F., D. I. Bounous, J. V. Kilburn, V. F. Nettles and R. D. Wyatt. (2000). The effect of dietary aflatoxin on wild turkey poults. Journal of Wildlife Diseases. 36 (3): 436-444.
[48] Rajani, P., V. Sridevi, M. V. V. C. Lakshmi and S. P. K. Kumari. (2012). Inhibitory effect of aqueous plant extracts on the growth of aflatoxin producing Aspergillus parasiticus (NCIM 898). International Journal of Engineering Science and Advanced Technology. 2 (2): 365-371.
[49] Rashid, N., M. A. Bajwa, M. Rafeeq, M. M. Tariq, F. Abbas, M. A. Awan, M. A. Khan, I. Shahzad, A. Rehman and Z. Ahmad. (2013). Prevalence of Aflatoxicosis in broiler chickens in Quetta, Pakistan. Pakistan Journal of Zoology. 45 (4): 1021-1026.
[50] Rawal, S., J. E. Kim and R. C. Jr. (2010). Aflatoxin B1 in poultry: Toxicology, metabolism and prevention. Research in Veterinary Science. 89: 325-331.
[51] Reiter E., Zentek J., Razzazi E. (2009). Review on sample preparation strategies and methods used for the analysis of aflatoxins in food and feed. Mol. Nutr. Food Res. 53 508–524.
[52] Roger, A. and J. Coulombe. (1993). Biological action of mycotoxins. Journal of Dairy Science. 76: 880-891.
[53] Romani L. (2004). Immunity to fungal infections. Nat. Rev. Immunol. 4 1–23.
[54] S. Ahmad, F. Ali and M. S. Hameed.(2017). Role of aflR Gene Expression from A. flavus to Cause Disease in Human. Journal of Bacteriology & Mycology. 5 (2): 1-3.
[55] Safara, M., F. Zaini. S. J. Hashemi, M. Mahmoudi, A. R. Khosravi, F. S. Aliabadi. (2010). Aflatoxin detoxification in rice using citric acid. Iranian Journal of Public Health. 39 (2): 24-29.
[56] Saini, S. S. and A. Kaur. (2012). Aflatoxin B1: Toxicity, characteristics and analysis: mini review. Global Advanced Research Journal of Chemistry and Material Science. 1 (4): 63-70.
[57] Shadbad, S., M. Reza, A. Masoud, T. Ali, G. Faranak and N. Mahboob. (2012). Determination of aflatoxins in nuts of Tabriz confectionaries by ELISA and HPLC methods. Advanced Pharmaceutical Bulletin. 2 (1): 123-126.
[58] Shi, Y. H., Z. R. Xu, J. L. Feng and C. Z. Wang. (2006). Efficacy of modified montmorillonite nanocomposite to reduce the toxicity of aflatoxin in broiler chicks. Animal Feed Science and Technology. 129: 138-148.
[59] Shuaib, F. M. B., J. Ehiri, A. Abdullahi, J. H. Williams and P. E. Jolly. (2010). Reproductive health effects of aflatoxins: A review of the literature. Reproductive Toxicology. 29: 262-270.
[60] Stewart, R. K., C. J. S. Singh and T. Massey. (1996). Glutathione S-transferase-catalyzed conjugation of bioactivated aflatoxin B1 in rabbit lung and liver. Toxicology and Applied Pharmacology. 140: 499-507.
[61] Sweeney, T. (2002). Is exposure to endocrine disrupting compounds during fetal/post-natal development affecting the reproductive potential of farm animals. Domestic Animal Endocrinology. 23: 203-209.
[62] Tripathi, S. and H. N. Mishra. (2009). Nutritional changes in powdered red pepper upon in-vitro infection of Aspergillus flavus. Brazilian Journal of Microbiology. 40 (1): 139-144.
[63] Velazhahan, R., S. Vijayanandraj, A. Vijayasamundeeswari, V. Paranidharan, R. Samiyappan, T. Iwamoto, B. Friebe and S. Muthukrishnan. (2010 (Detoxification of aflatoxins by seed extracts of the medicinal plant, Trachyspermum ammi (L.) Sprague ex Turrill – Structural analysis and biological toxicity of degradation product of aflatoxin G1. Food Control. 21: 719–725.
[64] Verma, R. J. (2004). Aflatoxin cause DNA damage. International Journal of Human Genetics. 4 (4): 231-236.
[65] Villa, P. and P. Markaki. (2009). Aflatoxin B1 and ochratoxin A in breakfast cereals from Athens market: Occurrence and risk assessment. Food Control. 20 (5): 455-461.
[66] Yabe K., Nakajima H. (2004). Enzyme reactions and genes in aflatoxin biosynthesis. Appl. Microbiol. Biotechnol. 64 745–755.
[67] Younis, Y. M. H. and K. M. Malik. (2003). TLC and HPLC assaya of aflatoxin contamination in Sudanese peanuts and peanut products. Kuwait Journal of Science and Engineering. 30 (1): 79-94.
[68] Z. Nemeth, A. P. Molnar, B. Fejes, L. Novak, L. Karaffa, N. P. Kelle and E. Fekete. (2016). Growth-Phase Sterigmatocystin Formation on LactoseIs Mediated via Low Specific Growth Rates inAspergillus nidulans. Toxins. 8: 1-14.
[69] Zeng, R. S., G. Niu, Z. Wen, M. A. Schuler, M. R. Berenbaum. (2006). Toxicity of aflatoxin B1 to Helicoverpa zea and bioactivation by cytochrome P450 monooxygenases. Journal of Chemical Ecology. 32: 1459-1471.
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  • APA Style

    Syeda Mona Hassan, Shahzad Sharif Mughal, Syed Khurram Hassan, Asif Ibrahim, Huma Hassan. (2020). Cellular Interactions, Metabolism, Assessment and Control of Aflatoxins: An Update. Computational Biology and Bioinformatics, 8(2), 62-71. https://doi.org/10.11648/j.cbb.20200802.15

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    ACS Style

    Syeda Mona Hassan; Shahzad Sharif Mughal; Syed Khurram Hassan; Asif Ibrahim; Huma Hassan. Cellular Interactions, Metabolism, Assessment and Control of Aflatoxins: An Update. Comput. Biol. Bioinform. 2020, 8(2), 62-71. doi: 10.11648/j.cbb.20200802.15

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    AMA Style

    Syeda Mona Hassan, Shahzad Sharif Mughal, Syed Khurram Hassan, Asif Ibrahim, Huma Hassan. Cellular Interactions, Metabolism, Assessment and Control of Aflatoxins: An Update. Comput Biol Bioinform. 2020;8(2):62-71. doi: 10.11648/j.cbb.20200802.15

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  • @article{10.11648/j.cbb.20200802.15,
      author = {Syeda Mona Hassan and Shahzad Sharif Mughal and Syed Khurram Hassan and Asif Ibrahim and Huma Hassan},
      title = {Cellular Interactions, Metabolism, Assessment and Control of Aflatoxins: An Update},
      journal = {Computational Biology and Bioinformatics},
      volume = {8},
      number = {2},
      pages = {62-71},
      doi = {10.11648/j.cbb.20200802.15},
      url = {https://doi.org/10.11648/j.cbb.20200802.15},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.cbb.20200802.15},
      abstract = {Aspergillus spp., the fungus containing aflatoxin, is commonly spread in nature and contains severely polluted food sources from humans and wildlife, resulting in risks to health and even mortality of species. In plants, such as maize and peanuts, the spores of Aspergillus paraciticus and Aspergillus flavus can grow on the surface of stigma. The germ tube goes into the developing embryo and mimics pollen germ tubes. Aflatoxins are naturally occurring substances, so it is difficult to remove them completely from products. However, they should be lessened to minimum possible level. There is also a strong need for research into aflatoxins to establish effective methods for their correct identification , quantification and monitoring to ensure public health safety. The chemistry and biosynthesis process of aflatoxins is addressed in a succinct fashion along with their occurrence and the toxic health threats to humans and livestock. This analysis focuses primarily on aflatoxin tools, development, identification and control techniques to ensure food and feed safety. The study is very useful to health-conscious customers and academic authorities in the related fields. In addition , the availability of information on toxicity of aflatoxins would help ensure food safety and solve potential problems for the rising population by reducing the occurrence of outbreaks related to aflatoxins.},
     year = {2020}
    }
    

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    T1  - Cellular Interactions, Metabolism, Assessment and Control of Aflatoxins: An Update
    AU  - Syeda Mona Hassan
    AU  - Shahzad Sharif Mughal
    AU  - Syed Khurram Hassan
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    AU  - Huma Hassan
    Y1  - 2020/11/19
    PY  - 2020
    N1  - https://doi.org/10.11648/j.cbb.20200802.15
    DO  - 10.11648/j.cbb.20200802.15
    T2  - Computational Biology and Bioinformatics
    JF  - Computational Biology and Bioinformatics
    JO  - Computational Biology and Bioinformatics
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    EP  - 71
    PB  - Science Publishing Group
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    UR  - https://doi.org/10.11648/j.cbb.20200802.15
    AB  - Aspergillus spp., the fungus containing aflatoxin, is commonly spread in nature and contains severely polluted food sources from humans and wildlife, resulting in risks to health and even mortality of species. In plants, such as maize and peanuts, the spores of Aspergillus paraciticus and Aspergillus flavus can grow on the surface of stigma. The germ tube goes into the developing embryo and mimics pollen germ tubes. Aflatoxins are naturally occurring substances, so it is difficult to remove them completely from products. However, they should be lessened to minimum possible level. There is also a strong need for research into aflatoxins to establish effective methods for their correct identification , quantification and monitoring to ensure public health safety. The chemistry and biosynthesis process of aflatoxins is addressed in a succinct fashion along with their occurrence and the toxic health threats to humans and livestock. This analysis focuses primarily on aflatoxin tools, development, identification and control techniques to ensure food and feed safety. The study is very useful to health-conscious customers and academic authorities in the related fields. In addition , the availability of information on toxicity of aflatoxins would help ensure food safety and solve potential problems for the rising population by reducing the occurrence of outbreaks related to aflatoxins.
    VL  - 8
    IS  - 2
    ER  - 

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Author Information
  • Department of Chemistry, Lahore Garrison University, Lahore, Pakistan

  • Department of Chemistry, Lahore Garrison University, Lahore, Pakistan

  • Institute of Quality and Technology Management, University of the Punjab, Lahore, Pakistan

  • Department of Mathematics, Lahore Garrison University, Lahore, Pakistan

  • Department of Chemical Engineering, NFC Institute of Engineering and Fertilizer Research, Faisalabad, Pakistan

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