Peripheral Blood Lymphocyte Subsets Counts in Children on Regular Hemodialysis

Manal Abd El-Salam; Shaimaa Abdelmalik Pessar

doi:doi:10.11648/j.iji.20150301.11

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Peripheral Blood Lymphocyte Subsets Counts in Children on Regular Hemodialysis

Manal Abd El-Salam, Shaimaa Abdelmalik Pessar

Received: 17 October 2014 Accepted: 31 October 2014 Published: 10 November 2014

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Abstract

Background: Hemodialysis (HD) procedure per se as well as disturbances in both innate and adaptive immunity makes HD patients susceptible to infections. Infections are the major cause of morbidity and the second cause of death following cardiovascular events in HD patients. Aim: Was to study lymphocyte subset counts in children with chronic kidney disease on regular hemodialysis in comparison with normal subjects to clarify the abnormalities in cellular immune profile. Patients and methods: The study included 40children with chronic kidney disease on regular hemodialysis and 20 healthy control (HC) children age and sex matched as a control group, they were selected from the pediatric hemodialysis unit and out patients clinic of AL-zahraa hospital ,Al-Azher university during the period from September 2013to June 2014. We examined the number of the peripheral lymphocytes. Also quantification of (T CD3+ & B CD19+) lymphocytes and T cell subsets including T helper CD3+CD4+, T cytotoxic CD3+ CD8+, CD4/CD8 ratio, and NK cells CD3-CD56+ using flow cytometric analysis and correlates their number with clinical and laboratory characteristics. Results: The total peripheral blood lymphocyte count was lower in HD children (2.3 x10³/uL) than HC children (3 x10³/uL), also T lymphocytes CD3+ counts were reduced in HD children (1609.25 ±545.77 / uL) than HC (2114.20± 868.39 /uL), (p=0.008), numbers of CD4+ T cells were not different, but numbers of CD8+ T cells were lower in HD children (600.67±284.92) compared with HC (896.80±573.00) (p < 0.05). Decrease in NK cell counts CD3-CD56+ in HD children (175.35±107.44) in comparison to HC (271.30±169.94). The B lymphocyte count CD19+ was not different in HD children (260±201.6) compared with healthy controls (250.20±122.84). A positive correlation was found between B lymphocytes CD19+ with hemodialysis duration. A negative correlation between NK cell counts and BUN, age and also with patients weight and height were found. Conclusion: The reduced numbers of lymphocyte count, T lymphocytes CD3+counts, CD8+ T cells as well as NK cell in CKD children on regular hemodialysis may favor the frequent occurrence of infections in spit the normal number of the other studied t cell subsets.

DOI	10.11648/j.iji.20150301.11
Published in	International Journal of Immunology (Volume 3, Issue 1, February 2015)
Page(s)	1-6
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Hemodialysis, Children, CD4, CD8, CD56, NK Cell

References

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APA Style

Manal Abd El-Salam, Shaimaa Abdelmalik Pessar. (2014). Peripheral Blood Lymphocyte Subsets Counts in Children on Regular Hemodialysis. International Journal of Immunology, 3(1), 1-6. https://doi.org/10.11648/j.iji.20150301.11

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Manal Abd El-Salam; Shaimaa Abdelmalik Pessar. Peripheral Blood Lymphocyte Subsets Counts in Children on Regular Hemodialysis. Int. J. Immunol. 2014, 3(1), 1-6. doi: 10.11648/j.iji.20150301.11

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AMA Style

Manal Abd El-Salam, Shaimaa Abdelmalik Pessar. Peripheral Blood Lymphocyte Subsets Counts in Children on Regular Hemodialysis. Int J Immunol. 2014;3(1):1-6. doi: 10.11648/j.iji.20150301.11

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@article{10.11648/j.iji.20150301.11,
  author = {Manal Abd El-Salam and Shaimaa Abdelmalik Pessar},
  title = {Peripheral Blood Lymphocyte Subsets Counts in Children on Regular Hemodialysis},
  journal = {International Journal of Immunology},
  volume = {3},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.iji.20150301.11},
  url = {https://doi.org/10.11648/j.iji.20150301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20150301.11},
  abstract = {Background: Hemodialysis (HD) procedure per se as well as disturbances in both innate and adaptive immunity makes HD patients susceptible to infections. Infections are the major cause of morbidity and the second cause of death following cardiovascular events in HD patients. Aim: Was to study lymphocyte subset counts in children with chronic kidney disease on regular hemodialysis in comparison with normal subjects to clarify the abnormalities in cellular immune profile. Patients and methods: The study included 40children with chronic kidney disease on regular hemodialysis and 20 healthy control (HC) children age and sex matched as a control group, they were selected from the pediatric hemodialysis unit and out patients clinic of AL-zahraa hospital ,Al-Azher university during the period from September 2013to June 2014. We examined the number of the peripheral lymphocytes. Also quantification of (T CD3+ & B CD19+) lymphocytes and T cell subsets including T helper CD3+CD4+, T cytotoxic CD3+ CD8+, CD4/CD8 ratio, and NK cells CD3-CD56+ using flow cytometric analysis and correlates their number with clinical and laboratory characteristics. Results: The total peripheral blood lymphocyte count was lower in HD children (2.3 x10³/uL) than HC children (3 x10³/uL), also T lymphocytes CD3+ counts were reduced in HD children (1609.25 ±545.77 / uL) than HC (2114.20± 868.39 /uL), (p=0.008), numbers of CD4+ T cells were not different, but numbers of CD8+ T cells were lower in HD children (600.67±284.92) compared with HC  (896.80±573.00) (p < 0.05). Decrease in NK cell counts CD3-CD56+ in HD children (175.35±107.44) in comparison to HC (271.30±169.94). The B lymphocyte count CD19+ was not different in HD children (260±201.6) compared with healthy controls (250.20±122.84). A positive correlation was found between B lymphocytes CD19+ with hemodialysis duration. A negative correlation between NK cell counts and BUN, age and also with patients weight and height were found. Conclusion: The reduced numbers of lymphocyte count, T lymphocytes CD3+counts, CD8+ T cells as well as NK cell in CKD children on regular hemodialysis may favor the frequent occurrence of infections in spit the normal number of the other studied t cell subsets.},
 year = {2014}
}

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TY  - JOUR
T1  - Peripheral Blood Lymphocyte Subsets Counts in Children on Regular Hemodialysis
AU  - Manal Abd El-Salam
AU  - Shaimaa Abdelmalik Pessar
Y1  - 2014/11/10
PY  - 2014
N1  - https://doi.org/10.11648/j.iji.20150301.11
DO  - 10.11648/j.iji.20150301.11
T2  - International Journal of Immunology
JF  - International Journal of Immunology
JO  - International Journal of Immunology
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2329-1753
UR  - https://doi.org/10.11648/j.iji.20150301.11
AB  - Background: Hemodialysis (HD) procedure per se as well as disturbances in both innate and adaptive immunity makes HD patients susceptible to infections. Infections are the major cause of morbidity and the second cause of death following cardiovascular events in HD patients. Aim: Was to study lymphocyte subset counts in children with chronic kidney disease on regular hemodialysis in comparison with normal subjects to clarify the abnormalities in cellular immune profile. Patients and methods: The study included 40children with chronic kidney disease on regular hemodialysis and 20 healthy control (HC) children age and sex matched as a control group, they were selected from the pediatric hemodialysis unit and out patients clinic of AL-zahraa hospital ,Al-Azher university during the period from September 2013to June 2014. We examined the number of the peripheral lymphocytes. Also quantification of (T CD3+ & B CD19+) lymphocytes and T cell subsets including T helper CD3+CD4+, T cytotoxic CD3+ CD8+, CD4/CD8 ratio, and NK cells CD3-CD56+ using flow cytometric analysis and correlates their number with clinical and laboratory characteristics. Results: The total peripheral blood lymphocyte count was lower in HD children (2.3 x10³/uL) than HC children (3 x10³/uL), also T lymphocytes CD3+ counts were reduced in HD children (1609.25 ±545.77 / uL) than HC (2114.20± 868.39 /uL), (p=0.008), numbers of CD4+ T cells were not different, but numbers of CD8+ T cells were lower in HD children (600.67±284.92) compared with HC  (896.80±573.00) (p < 0.05). Decrease in NK cell counts CD3-CD56+ in HD children (175.35±107.44) in comparison to HC (271.30±169.94). The B lymphocyte count CD19+ was not different in HD children (260±201.6) compared with healthy controls (250.20±122.84). A positive correlation was found between B lymphocytes CD19+ with hemodialysis duration. A negative correlation between NK cell counts and BUN, age and also with patients weight and height were found. Conclusion: The reduced numbers of lymphocyte count, T lymphocytes CD3+counts, CD8+ T cells as well as NK cell in CKD children on regular hemodialysis may favor the frequent occurrence of infections in spit the normal number of the other studied t cell subsets.
VL  - 3
IS  - 1
ER  -

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Author Information

Manal Abd El-Salam

Department of Pediatrics, Faculty of Medicine (for girls), Al-Azhar University, Cairo, Egypt
Shaimaa Abdelmalik Pessar

Department of Clinical and chemical pathology, Faculty of Medicine Ain Shams University, Cairo, Egypt

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APA Style

Manal Abd El-Salam, Shaimaa Abdelmalik Pessar. (2014). Peripheral Blood Lymphocyte Subsets Counts in Children on Regular Hemodialysis. International Journal of Immunology, 3(1), 1-6. https://doi.org/10.11648/j.iji.20150301.11

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ACS Style

Manal Abd El-Salam; Shaimaa Abdelmalik Pessar. Peripheral Blood Lymphocyte Subsets Counts in Children on Regular Hemodialysis. Int. J. Immunol. 2014, 3(1), 1-6. doi: 10.11648/j.iji.20150301.11

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AMA Style

Manal Abd El-Salam, Shaimaa Abdelmalik Pessar. Peripheral Blood Lymphocyte Subsets Counts in Children on Regular Hemodialysis. Int J Immunol. 2014;3(1):1-6. doi: 10.11648/j.iji.20150301.11

Copy | Download

@article{10.11648/j.iji.20150301.11,
  author = {Manal Abd El-Salam and Shaimaa Abdelmalik Pessar},
  title = {Peripheral Blood Lymphocyte Subsets Counts in Children on Regular Hemodialysis},
  journal = {International Journal of Immunology},
  volume = {3},
  number = {1},
  pages = {1-6},
  doi = {10.11648/j.iji.20150301.11},
  url = {https://doi.org/10.11648/j.iji.20150301.11},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20150301.11},
  abstract = {Background: Hemodialysis (HD) procedure per se as well as disturbances in both innate and adaptive immunity makes HD patients susceptible to infections. Infections are the major cause of morbidity and the second cause of death following cardiovascular events in HD patients. Aim: Was to study lymphocyte subset counts in children with chronic kidney disease on regular hemodialysis in comparison with normal subjects to clarify the abnormalities in cellular immune profile. Patients and methods: The study included 40children with chronic kidney disease on regular hemodialysis and 20 healthy control (HC) children age and sex matched as a control group, they were selected from the pediatric hemodialysis unit and out patients clinic of AL-zahraa hospital ,Al-Azher university during the period from September 2013to June 2014. We examined the number of the peripheral lymphocytes. Also quantification of (T CD3+ & B CD19+) lymphocytes and T cell subsets including T helper CD3+CD4+, T cytotoxic CD3+ CD8+, CD4/CD8 ratio, and NK cells CD3-CD56+ using flow cytometric analysis and correlates their number with clinical and laboratory characteristics. Results: The total peripheral blood lymphocyte count was lower in HD children (2.3 x10³/uL) than HC children (3 x10³/uL), also T lymphocytes CD3+ counts were reduced in HD children (1609.25 ±545.77 / uL) than HC (2114.20± 868.39 /uL), (p=0.008), numbers of CD4+ T cells were not different, but numbers of CD8+ T cells were lower in HD children (600.67±284.92) compared with HC  (896.80±573.00) (p < 0.05). Decrease in NK cell counts CD3-CD56+ in HD children (175.35±107.44) in comparison to HC (271.30±169.94). The B lymphocyte count CD19+ was not different in HD children (260±201.6) compared with healthy controls (250.20±122.84). A positive correlation was found between B lymphocytes CD19+ with hemodialysis duration. A negative correlation between NK cell counts and BUN, age and also with patients weight and height were found. Conclusion: The reduced numbers of lymphocyte count, T lymphocytes CD3+counts, CD8+ T cells as well as NK cell in CKD children on regular hemodialysis may favor the frequent occurrence of infections in spit the normal number of the other studied t cell subsets.},
 year = {2014}
}

Copy | Download

TY  - JOUR
T1  - Peripheral Blood Lymphocyte Subsets Counts in Children on Regular Hemodialysis
AU  - Manal Abd El-Salam
AU  - Shaimaa Abdelmalik Pessar
Y1  - 2014/11/10
PY  - 2014
N1  - https://doi.org/10.11648/j.iji.20150301.11
DO  - 10.11648/j.iji.20150301.11
T2  - International Journal of Immunology
JF  - International Journal of Immunology
JO  - International Journal of Immunology
SP  - 1
EP  - 6
PB  - Science Publishing Group
SN  - 2329-1753
UR  - https://doi.org/10.11648/j.iji.20150301.11
AB  - Background: Hemodialysis (HD) procedure per se as well as disturbances in both innate and adaptive immunity makes HD patients susceptible to infections. Infections are the major cause of morbidity and the second cause of death following cardiovascular events in HD patients. Aim: Was to study lymphocyte subset counts in children with chronic kidney disease on regular hemodialysis in comparison with normal subjects to clarify the abnormalities in cellular immune profile. Patients and methods: The study included 40children with chronic kidney disease on regular hemodialysis and 20 healthy control (HC) children age and sex matched as a control group, they were selected from the pediatric hemodialysis unit and out patients clinic of AL-zahraa hospital ,Al-Azher university during the period from September 2013to June 2014. We examined the number of the peripheral lymphocytes. Also quantification of (T CD3+ & B CD19+) lymphocytes and T cell subsets including T helper CD3+CD4+, T cytotoxic CD3+ CD8+, CD4/CD8 ratio, and NK cells CD3-CD56+ using flow cytometric analysis and correlates their number with clinical and laboratory characteristics. Results: The total peripheral blood lymphocyte count was lower in HD children (2.3 x10³/uL) than HC children (3 x10³/uL), also T lymphocytes CD3+ counts were reduced in HD children (1609.25 ±545.77 / uL) than HC (2114.20± 868.39 /uL), (p=0.008), numbers of CD4+ T cells were not different, but numbers of CD8+ T cells were lower in HD children (600.67±284.92) compared with HC  (896.80±573.00) (p < 0.05). Decrease in NK cell counts CD3-CD56+ in HD children (175.35±107.44) in comparison to HC (271.30±169.94). The B lymphocyte count CD19+ was not different in HD children (260±201.6) compared with healthy controls (250.20±122.84). A positive correlation was found between B lymphocytes CD19+ with hemodialysis duration. A negative correlation between NK cell counts and BUN, age and also with patients weight and height were found. Conclusion: The reduced numbers of lymphocyte count, T lymphocytes CD3+counts, CD8+ T cells as well as NK cell in CKD children on regular hemodialysis may favor the frequent occurrence of infections in spit the normal number of the other studied t cell subsets.
VL  - 3
IS  - 1
ER  -

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