Impact of Biofield Energy Treated Herbomineral Formulation (The Trivedi Effect®) on Mouse Dendritic and Splenocyte Cells for Modulation of Pro-inflammatory Cytokines
International Journal of Immunology
Volume 4, Issue 5, October 2016, Pages: 35-45
Received: Nov. 7, 2016; Accepted: Nov. 22, 2016; Published: Dec. 8, 2016
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Authors
Mahendra Kumar Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Alice Branton, Trivedi Global, Inc., Henderson, Nevada, USA
Dahryn Trivedi, Trivedi Global, Inc., Henderson, Nevada, USA
Gopal Nayak, Trivedi Global, Inc., Henderson, Nevada, USA
Aileen Carol Lee, Trivedi Global, Inc., Henderson, Nevada, USA
Aksana Hancharuk, Trivedi Global, Inc., Henderson, Nevada, USA
Carola Marina Sand, Trivedi Global, Inc., Henderson, Nevada, USA
Debra Jane Schnitzer, Trivedi Global, Inc., Henderson, Nevada, USA
Rudina Thanasi, Trivedi Global, Inc., Henderson, Nevada, USA
Eileen Mary Meagher, Trivedi Global, Inc., Henderson, Nevada, USA
Faith Ann Pyka, Trivedi Global, Inc., Henderson, Nevada, USA
Gary Richard Gerber, Trivedi Global, Inc., Henderson, Nevada, USA
Johanna Catharina Stromsnas, Trivedi Global, Inc., Henderson, Nevada, USA
Judith Marian Shapiro, Trivedi Global, Inc., Henderson, Nevada, USA
Laura Nelson Streicher, Trivedi Global, Inc., Henderson, Nevada, USA
Lorraine Marie Hachfeld, Trivedi Global, Inc., Henderson, Nevada, USA
Matthew Charles Hornung, Trivedi Global, Inc., Henderson, Nevada, USA
Patricia M. Rowe, Trivedi Global, Inc., Henderson, Nevada, USA
Sally Jean Henderson, Trivedi Global, Inc., Henderson, Nevada, USA
Sheila Maureen Benson, Trivedi Global, Inc., Henderson, Nevada, USA
Shirley Theresa Holmlund, Trivedi Global, Inc., Henderson, Nevada, USA
Stephen P. Salters, Trivedi Global, Inc., Henderson, Nevada, USA
Mayank Gangwar, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
Snehasis Jana, Trivedi Science Research Laboratory Pvt. Ltd., Bhopal, Madhya Pradesh, India
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Abstract
The use of herbomineral formulation in the healthcare sector for different chronic diseases is gaining popularity due to its fewer side effects, high safety profile, and cost effectiveness. A new proprietary herbomineral formulation was formulated, consisting of four essential ingredients viz. herbal root extract (ashwagandha), and minerals (zinc, magnesium, and selenium). The study aims to evaluate the in vitro effect of Biofield Energy Healing (The Trivedi Effect®) on the test formulation using murine dendritic (DCs) and splenocyte cells. The herbomineral formulation was divided into two parts; one was represented as control, while the other part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers (The Trivedi Effect®) and defined as the Biofield Treated formulation. The effect of the test formulation on these cells were monitored by an estimation of pro-inflammatory cytokines level such as tumor necrosis factor (TNF-α), macrophage inflammatory protein (MIP-1α), and interleukin (IL-1β) in cell culture supernatants at the non-cytotoxic concentrations of the test formulation using MTT assay. The DCs were treated with the Biofield Energy Treated test formulation at different concentrations (i.e. 1.05 to 1052.5 µg/mL) for 24 hours, and the results showed significant (p≤0.001) suppression of TNF-α levels at all the tested concentrations with a maximum percentage decrease by 43.64% at 5.2 µg/mL concentration in the Biofield Treated formulation as compared with the untreated test formulation. Further, the Biofield Treated formulation also demonstrated inhibition of MIP-1α and IL-1β at a concentration range of 0.0000105 to 10.5 µg/mL in LPS stimulated splenocyte cells. There was a significant (p≤0.001) inhibition of MIP-1α (26.52%) and IL-1β (35.28%) in the Biofield Treated test formulation at concentration 0.000105 µg/mL and 0.0000105 µg/mL, respectively in comparison to the untreated test formulation. Overall, these results suggest that the Biofield Energy Healing based herbomineral formulation (The Trivedi Effect®) significantly down-regulated the tested cytokines expression in DCs and splenocyte cells as compared to the untreated formulation. Therefore, the Biofield Healing based formulation might be useful as a better anti-inflammatory product for many chronic and acute inflammatory disease conditions and autoimmune disorders. The Biofield Energy Treatment based formulation can also be effectively applied in cases of organ transplants, stress management and anti-aging by improving overall health.
Keywords
Biofield Energy Healing Treatment, Biofield Energy Healers, The Trivedi Effect®, Herbomineral Formulation, Immune-Modulation, Pro-Inflammatory Cytokines, Dendritic Cells, Splenocytes
To cite this article
Mahendra Kumar Trivedi, Alice Branton, Dahryn Trivedi, Gopal Nayak, Aileen Carol Lee, Aksana Hancharuk, Carola Marina Sand, Debra Jane Schnitzer, Rudina Thanasi, Eileen Mary Meagher, Faith Ann Pyka, Gary Richard Gerber, Johanna Catharina Stromsnas, Judith Marian Shapiro, Laura Nelson Streicher, Lorraine Marie Hachfeld, Matthew Charles Hornung, Patricia M. Rowe, Sally Jean Henderson, Sheila Maureen Benson, Shirley Theresa Holmlund, Stephen P. Salters, Mayank Gangwar, Snehasis Jana, Impact of Biofield Energy Treated Herbomineral Formulation (The Trivedi Effect®) on Mouse Dendritic and Splenocyte Cells for Modulation of Pro-inflammatory Cytokines, International Journal of Immunology. Vol. 4, No. 5, 2016, pp. 35-45. doi: 10.11648/j.iji.20160405.12
Copyright
Copyright © 2016 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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