Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire

Goran-Kouacou Amah Patricia Victorine; Siransy Kouabla Liliane; Adou Adjoumanvoulé Honoré; Yéboah Oppong Richard; Guina Denise Affoué Blassonny; Sékongo Yassongui Mamadou; N’Guessan Koffi; Dassé Séry Romuald

doi:doi:10.11648/j.iji.20160406.14

Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire

Goran-Kouacou Amah Patricia Victorine, Siransy Kouabla Liliane, Adou Adjoumanvoulé Honoré, Yéboah Oppong Richard, Guina Denise Affoué Blassonny, Sékongo Yassongui Mamadou, N’Guessan Koffi, Dassé Séry Romuald

Published in International Journal of Immunology (Volume 4, Issue 6)

Received: 11 December 2016 Accepted: 26 December 2016 Published: 18 January 2017

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Abstract

ABO blood-group system is characterized by the constant presence in the plasma of natural anti-A and anti-B antibodies (regular, agglutinating, class IgM) corresponding to the antigens absent on the membrane of the red blood cell. In addition to these natural antibodies, there may be immune antibodies (called haemolysins) in response to different types of immunological stimuli. These anti-A and anti-B haemolysins are capable of triggering the complete cascade of complement leading to haemolytic accidents in the case of non-isogroup ABO transfusion. Our objective was to determine the frequency of IgG anti-A, anti-B and anti-A + B haemolysins in group O blood donors at the national blood transfusion center of Abidjan. Our retrospective cross-sectional study was conducted at the national blood transfusion center of Abidjan and that investigated anti-A, anti-B and anti-A + B haemolysins in the sera of 191 group O blood donnors, aged 18-65 years, using the heat technique with direct agglutination of the serum to be tested by red blood cells A1 and B papained. The prevalence of IgG haemolysins was 35.08% (10.47% anti-A, 15.71% anti-B and 8.9% anti-A + B). The average age of our population was 32,9 years [18-63 years] with a male / female sex ratio of 4.97. Haemolysin levels were higher in men (27.75%) than in women (7.33%) (not a significant difference, p= 0.44). Age distribution showed a high haemolysin level in the 25-29 age group (significant difference, p = 0.001), with variable rate ranging from 2.1% to 6.3%. Ideally, group O blood should only be transfused to group O subjects, except in emergency situations where iso-group blood is not available. Since the technique used in our study to investigated haemolysins was a qualitative method, we could not have the title of the various haemolysins in order to assess the real risk of an immunological accident after such a blood transfusion.

Published in	International Journal of Immunology (Volume 4, Issue 6)
DOI	10.11648/j.iji.20160406.14
Page(s)	68-72
Creative Commons	This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.
Copyright	Copyright © The Author(s), 2024. Published by Science Publishing Group

Keywords

Haemolysins, Blood Donors, Group O, Côte d'Ivoire

References

[1]	Lefrère JJ, Rouger Ph. Transfusion sanguine, 4ème édition, Masson, 2011.
[2]	Louati N, Cherif J, Ben Amor I, Rekik H, Gargouri J. Recherche des hémolysines chez les donneurs de sang. Journal de l’Information Médicale de Sfax Juin-Déc 2008; 15-16: 17-9.
[3]	Uko EK, Erhabor O, Ahmed HM, Isaac IZ, Abdulrahaman Y, Wase A, Ezimah A, Aghedo F Ikuenbor DB, Udomah FP, Iweke IP, Adias TC. Prevalence of high titre alpha and beta haemolysins among blood donors in Sokoto, North Western Nigeria. International Journal of Medical Sciences and Health Care Dec 2013; 1 (11): 1-7.
[4]	Giraud Ch. Korach JM, Andreu G, Lacaze C, Vaicle M, Schooneman F, Guillevin L. Les bases immunologiques de la transfusion. Transfusion Clinique et Biologique Juillet 2002; 9 (3): 163-7.
[5]	Kulkarni AG, Ibazebe R, Fleming AF. High frequency of anti-A and anti-B haemolysins in certain ethnic groups of Nigeria. Vox Sanguinis 1985; 48 (1): 39-41.
[6]	Olawumi HO, Olatunji PO. Prevalence and titre of alpha and beta haemolysins in blood group ‘O’ donors in Ilorin,” African Journal of Medicine and Medical Sciences Dec 2001; 30 (4): 319-21.
[7]	Kagu MB, Sagir G. Ahmed, Aisha A. Mohammed, Waheed K. Moshood, Mohammed B. Malah, Jimoh M. Kehinde. Anti-A and Anti-B haemolysins amongst group “O” voluntary blood donors in Northeastern Nigeria. Journal of Transfusion 2010; Vol 2011, Article ID 302406, 3p.
[8]	Oyedeji OA, Adeyemo TA, Ogbenna AA, Akanmu AS. Prevalence of anti A and anti B hemolysis among blood group O donors in Lagos. Nigerian Journal of Clinical Practice, May-Jun 2015; 18 (3): 328-32.
[9]	Rouger Ph, Salmon Ch. Les allohémagglutinines du système ABO. Revue Française de Transfusion et d’Immuno-hématologie 1977; 20 (4): 623-5.
[10]	Fopa D, Tagny CT, Tebeu PM, Ndoumba A, Mbanya D. Recherche et titrage des hémolysines anti-A et anti-B chez les femmes en période du postpartum immédiat au Centre Hospitalier et Universitaire de Yaoundé. Africa Sanguine 2013; 16 (2): 4-8.
[11]	Ugah U, Ibekailo S, Mbamagu D. Rate of Haemolysins among Blood Donors in Abakaliki, Ebonyi State, Nigeria. Global Journal of Medicine Researches and Studies 2014; 1 (3): 61-5.

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Goran-Kouacou Amah Patricia Victorine, Siransy Kouabla Liliane, Adou Adjoumanvoulé Honoré, Yéboah Oppong Richard, Guina Denise Affoué Blassonny, et al. (2017). Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire. International Journal of Immunology, 4(6), 68-72. https://doi.org/10.11648/j.iji.20160406.14

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ACS Style

Goran-Kouacou Amah Patricia Victorine; Siransy Kouabla Liliane; Adou Adjoumanvoulé Honoré; Yéboah Oppong Richard; Guina Denise Affoué Blassonny, et al. Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire. Int. J. Immunol. 2017, 4(6), 68-72. doi: 10.11648/j.iji.20160406.14

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AMA Style

Goran-Kouacou Amah Patricia Victorine, Siransy Kouabla Liliane, Adou Adjoumanvoulé Honoré, Yéboah Oppong Richard, Guina Denise Affoué Blassonny, et al. Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire. Int J Immunol. 2017;4(6):68-72. doi: 10.11648/j.iji.20160406.14

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@article{10.11648/j.iji.20160406.14,
  author = {Goran-Kouacou Amah Patricia Victorine and Siransy Kouabla Liliane and Adou Adjoumanvoulé Honoré and Yéboah Oppong Richard and Guina Denise Affoué Blassonny and Sékongo Yassongui Mamadou and N’Guessan Koffi and Dassé Séry Romuald},
  title = {Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire},
  journal = {International Journal of Immunology},
  volume = {4},
  number = {6},
  pages = {68-72},
  doi = {10.11648/j.iji.20160406.14},
  url = {https://doi.org/10.11648/j.iji.20160406.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20160406.14},
  abstract = {ABO blood-group system is characterized by the constant presence in the plasma of natural anti-A and anti-B antibodies (regular, agglutinating, class IgM) corresponding to the antigens absent on the membrane of the red blood cell. In addition to these natural antibodies, there may be immune antibodies (called haemolysins) in response to different types of immunological stimuli. These anti-A and anti-B haemolysins are capable of triggering the complete cascade of complement leading to haemolytic accidents in the case of non-isogroup ABO transfusion. Our objective was to determine the frequency of IgG anti-A, anti-B and anti-A + B haemolysins in group O blood donors at the national blood transfusion center of Abidjan. Our retrospective cross-sectional study was conducted at the national blood transfusion center of Abidjan and that investigated anti-A, anti-B and anti-A + B haemolysins in the sera of 191 group O blood donnors, aged 18-65 years, using the heat technique with direct agglutination of the serum to be tested by red blood cells A1 and B papained. The prevalence of IgG haemolysins was 35.08% (10.47% anti-A, 15.71% anti-B and 8.9% anti-A + B). The average age of our population was 32,9 years [18-63 years] with a male / female sex ratio of 4.97. Haemolysin levels were higher in men (27.75%) than in women (7.33%) (not a significant difference, p= 0.44). Age distribution showed a high haemolysin level in the 25-29 age group (significant difference, p = 0.001), with variable rate ranging from 2.1% to 6.3%. Ideally, group O blood should only be transfused to group O subjects, except in emergency situations where iso-group blood is not available. Since the technique used in our study to investigated haemolysins was a qualitative method, we could not have the title of the various haemolysins in order to assess the real risk of an immunological accident after such a blood transfusion.},
 year = {2017}
}

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TY  - JOUR
T1  - Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire
AU  - Goran-Kouacou Amah Patricia Victorine
AU  - Siransy Kouabla Liliane
AU  - Adou Adjoumanvoulé Honoré
AU  - Yéboah Oppong Richard
AU  - Guina Denise Affoué Blassonny
AU  - Sékongo Yassongui Mamadou
AU  - N’Guessan Koffi
AU  - Dassé Séry Romuald
Y1  - 2017/01/18
PY  - 2017
N1  - https://doi.org/10.11648/j.iji.20160406.14
DO  - 10.11648/j.iji.20160406.14
T2  - International Journal of Immunology
JF  - International Journal of Immunology
JO  - International Journal of Immunology
SP  - 68
EP  - 72
PB  - Science Publishing Group
SN  - 2329-1753
UR  - https://doi.org/10.11648/j.iji.20160406.14
AB  - ABO blood-group system is characterized by the constant presence in the plasma of natural anti-A and anti-B antibodies (regular, agglutinating, class IgM) corresponding to the antigens absent on the membrane of the red blood cell. In addition to these natural antibodies, there may be immune antibodies (called haemolysins) in response to different types of immunological stimuli. These anti-A and anti-B haemolysins are capable of triggering the complete cascade of complement leading to haemolytic accidents in the case of non-isogroup ABO transfusion. Our objective was to determine the frequency of IgG anti-A, anti-B and anti-A + B haemolysins in group O blood donors at the national blood transfusion center of Abidjan. Our retrospective cross-sectional study was conducted at the national blood transfusion center of Abidjan and that investigated anti-A, anti-B and anti-A + B haemolysins in the sera of 191 group O blood donnors, aged 18-65 years, using the heat technique with direct agglutination of the serum to be tested by red blood cells A1 and B papained. The prevalence of IgG haemolysins was 35.08% (10.47% anti-A, 15.71% anti-B and 8.9% anti-A + B). The average age of our population was 32,9 years [18-63 years] with a male / female sex ratio of 4.97. Haemolysin levels were higher in men (27.75%) than in women (7.33%) (not a significant difference, p= 0.44). Age distribution showed a high haemolysin level in the 25-29 age group (significant difference, p = 0.001), with variable rate ranging from 2.1% to 6.3%. Ideally, group O blood should only be transfused to group O subjects, except in emergency situations where iso-group blood is not available. Since the technique used in our study to investigated haemolysins was a qualitative method, we could not have the title of the various haemolysins in order to assess the real risk of an immunological accident after such a blood transfusion.
VL  - 4
IS  - 6
ER  -

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Author Information

Goran-Kouacou Amah Patricia Victorine

Immunology and Allergology Department, Unit of Training and Research Medical Sciences in Abidjan, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire; Immunology and Hematology Service, University Hospital of Cocody, Abidjan, C?te d’Ivoire
Siransy Kouabla Liliane

Immunology and Allergology Department, Unit of Training and Research Medical Sciences in Abidjan, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire; National Blood Transfusion Center, Abidjan, C?te d’Ivoire
Adou Adjoumanvoulé Honoré

Immunology and Allergology Department, Unit of Training and Research Medical Sciences in Abidjan, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire; Immunology and Hematology Service, University Hospital of Cocody, Abidjan, C?te d’Ivoire
Yéboah Oppong Richard

Immunology and Allergology Department, Unit of Training and Research Medical Sciences in Abidjan, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire; Immunology and Hematology Service, University Hospital of Cocody, Abidjan, C?te d’Ivoire
Guina Denise Affoué Blassonny

Immunology and Hematology Service, University Hospital of Cocody, Abidjan, C?te d’Ivoire
Sékongo Yassongui Mamadou

National Blood Transfusion Center, Abidjan, C?te d’Ivoire; Hematology Clinic, University Hospital of Yopougon, Abidjan, C?te d’Ivoire
N’Guessan Koffi

Immunology and Allergology Department, Unit of Training and Research Medical Sciences in Abidjan, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire; Immunology and Hematology Service, University Hospital of Cocody, Abidjan, C?te d’Ivoire
Dassé Séry Romuald

Immunology and Allergology Department, Unit of Training and Research Medical Sciences in Abidjan, Félix Houphou?t-Boigny University, Abidjan, C?te d’Ivoire; Immunology and Hematology Service, University Hospital of Cocody, Abidjan, C?te d’Ivoire

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APA Style

Goran-Kouacou Amah Patricia Victorine, Siransy Kouabla Liliane, Adou Adjoumanvoulé Honoré, Yéboah Oppong Richard, Guina Denise Affoué Blassonny, et al. (2017). Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire. International Journal of Immunology, 4(6), 68-72. https://doi.org/10.11648/j.iji.20160406.14

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ACS Style

Goran-Kouacou Amah Patricia Victorine; Siransy Kouabla Liliane; Adou Adjoumanvoulé Honoré; Yéboah Oppong Richard; Guina Denise Affoué Blassonny, et al. Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire. Int. J. Immunol. 2017, 4(6), 68-72. doi: 10.11648/j.iji.20160406.14

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AMA Style

Goran-Kouacou Amah Patricia Victorine, Siransy Kouabla Liliane, Adou Adjoumanvoulé Honoré, Yéboah Oppong Richard, Guina Denise Affoué Blassonny, et al. Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire. Int J Immunol. 2017;4(6):68-72. doi: 10.11648/j.iji.20160406.14

Copy | Download

@article{10.11648/j.iji.20160406.14,
  author = {Goran-Kouacou Amah Patricia Victorine and Siransy Kouabla Liliane and Adou Adjoumanvoulé Honoré and Yéboah Oppong Richard and Guina Denise Affoué Blassonny and Sékongo Yassongui Mamadou and N’Guessan Koffi and Dassé Séry Romuald},
  title = {Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire},
  journal = {International Journal of Immunology},
  volume = {4},
  number = {6},
  pages = {68-72},
  doi = {10.11648/j.iji.20160406.14},
  url = {https://doi.org/10.11648/j.iji.20160406.14},
  eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.iji.20160406.14},
  abstract = {ABO blood-group system is characterized by the constant presence in the plasma of natural anti-A and anti-B antibodies (regular, agglutinating, class IgM) corresponding to the antigens absent on the membrane of the red blood cell. In addition to these natural antibodies, there may be immune antibodies (called haemolysins) in response to different types of immunological stimuli. These anti-A and anti-B haemolysins are capable of triggering the complete cascade of complement leading to haemolytic accidents in the case of non-isogroup ABO transfusion. Our objective was to determine the frequency of IgG anti-A, anti-B and anti-A + B haemolysins in group O blood donors at the national blood transfusion center of Abidjan. Our retrospective cross-sectional study was conducted at the national blood transfusion center of Abidjan and that investigated anti-A, anti-B and anti-A + B haemolysins in the sera of 191 group O blood donnors, aged 18-65 years, using the heat technique with direct agglutination of the serum to be tested by red blood cells A1 and B papained. The prevalence of IgG haemolysins was 35.08% (10.47% anti-A, 15.71% anti-B and 8.9% anti-A + B). The average age of our population was 32,9 years [18-63 years] with a male / female sex ratio of 4.97. Haemolysin levels were higher in men (27.75%) than in women (7.33%) (not a significant difference, p= 0.44). Age distribution showed a high haemolysin level in the 25-29 age group (significant difference, p = 0.001), with variable rate ranging from 2.1% to 6.3%. Ideally, group O blood should only be transfused to group O subjects, except in emergency situations where iso-group blood is not available. Since the technique used in our study to investigated haemolysins was a qualitative method, we could not have the title of the various haemolysins in order to assess the real risk of an immunological accident after such a blood transfusion.},
 year = {2017}
}

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TY  - JOUR
T1  - Prevalence of Anti-A and Anti-B Haemolysins in Group O Blood Donors at the National Blood Transfusion Center of Abidjan, Côte d'Ivoire
AU  - Goran-Kouacou Amah Patricia Victorine
AU  - Siransy Kouabla Liliane
AU  - Adou Adjoumanvoulé Honoré
AU  - Yéboah Oppong Richard
AU  - Guina Denise Affoué Blassonny
AU  - Sékongo Yassongui Mamadou
AU  - N’Guessan Koffi
AU  - Dassé Séry Romuald
Y1  - 2017/01/18
PY  - 2017
N1  - https://doi.org/10.11648/j.iji.20160406.14
DO  - 10.11648/j.iji.20160406.14
T2  - International Journal of Immunology
JF  - International Journal of Immunology
JO  - International Journal of Immunology
SP  - 68
EP  - 72
PB  - Science Publishing Group
SN  - 2329-1753
UR  - https://doi.org/10.11648/j.iji.20160406.14
AB  - ABO blood-group system is characterized by the constant presence in the plasma of natural anti-A and anti-B antibodies (regular, agglutinating, class IgM) corresponding to the antigens absent on the membrane of the red blood cell. In addition to these natural antibodies, there may be immune antibodies (called haemolysins) in response to different types of immunological stimuli. These anti-A and anti-B haemolysins are capable of triggering the complete cascade of complement leading to haemolytic accidents in the case of non-isogroup ABO transfusion. Our objective was to determine the frequency of IgG anti-A, anti-B and anti-A + B haemolysins in group O blood donors at the national blood transfusion center of Abidjan. Our retrospective cross-sectional study was conducted at the national blood transfusion center of Abidjan and that investigated anti-A, anti-B and anti-A + B haemolysins in the sera of 191 group O blood donnors, aged 18-65 years, using the heat technique with direct agglutination of the serum to be tested by red blood cells A1 and B papained. The prevalence of IgG haemolysins was 35.08% (10.47% anti-A, 15.71% anti-B and 8.9% anti-A + B). The average age of our population was 32,9 years [18-63 years] with a male / female sex ratio of 4.97. Haemolysin levels were higher in men (27.75%) than in women (7.33%) (not a significant difference, p= 0.44). Age distribution showed a high haemolysin level in the 25-29 age group (significant difference, p = 0.001), with variable rate ranging from 2.1% to 6.3%. Ideally, group O blood should only be transfused to group O subjects, except in emergency situations where iso-group blood is not available. Since the technique used in our study to investigated haemolysins was a qualitative method, we could not have the title of the various haemolysins in order to assess the real risk of an immunological accident after such a blood transfusion.
VL  - 4
IS  - 6
ER  -

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