Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/- Mutant Zebrafish
International Journal of Immunology
Volume 5, Issue 2, April 2017, Pages: 20-36
Received: Jan. 22, 2017; Accepted: Feb. 21, 2017; Published: Mar. 25, 2017
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Authors
Aparna Krishnavajhala, Department of Pediatrics, National School of Tropical Medicine, Baylor College of Medicine, Houston, Texas, USA
Preeti J. Muire, Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, USA
Larry Hanson, Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, USA
Henry Wan, Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, USA
Fiona McCarthy, School of Animal and Comparative Biomedical Sciences, University of Arizona, Tucson, USA; BIO5 Institute, University of Arizona, Tucson, USA
Alan Zhou, Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, USA
Lora Petrie-Hanson, Department of Basic Sciences, College of Veterinary Medicine, Mississippi State University, Mississippi State, USA
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Abstract
To elucidate the basis of protective immunity in T and B cell deficient rag1-/- mutant zebrafish, we conducted microarray analysis of 15,617 genes from rag1-/- mutant zebrafish 48 hours after a primary response and 48 hours after a secondary response. Following primary exposure, the highest fold expression differences (3.8 to 4.95) were genes for serum amyloid A, chemokine CCL-C5a (CCL-19a), signal transducer and activator of transcription (STAT) 1b, interferon regulatory factor 11, and myxovirus resistance A. Strong induction of these genes demonstrated that primary immune responses and innate immune cells were not impaired in T and B cell deficient mutant zebrafish. Following bacterial re-exposure, the highest fold expression differences (2 to 3 fold) were in chemokine CCL-C5a (CCL-19a), myomegalin, bone morphogenetic protein 4, and relaxin 3a. These genes are involved in the immune response and cell proliferation. Genes for cell receptor activation and signal transduction, cell proliferation and cytotoxic functions were also up-regulated. These findings suggest receptor activation and expansion of a cell population. Increased ifnγ expression at 48 hpi was associated with both primary and secondary immune responses.
Keywords
rag1-/- Mutant Zebrafish, Edwardsiella ictaluri, Protective Immunity, Transcriptome, Cell-Mediated Immunity
To cite this article
Aparna Krishnavajhala, Preeti J. Muire, Larry Hanson, Henry Wan, Fiona McCarthy, Alan Zhou, Lora Petrie-Hanson, Transcriptome Changes Associated with Protective Immunity in T and B Cell-Deficient Rag1-/- Mutant Zebrafish, International Journal of Immunology. Vol. 5, No. 2, 2017, pp. 20-36. doi: 10.11648/j.iji.20170502.11
Copyright
Copyright © 2017 Authors retain the copyright of this article.
This article is an open access article distributed under the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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